BACKGROUND:The patch materials, originaly applied in the reconstruction and repair of abdominal hernia, have been successfuly applied in the vagina surgery; thus, it has been gradualy generalized in gynecological pelvic floor reconstruction. OBJECTIVE:To analyze the clinical effect of polypropylene patch in older female patients with anterior pelvic organ reconstruction. METHODS:Totaly 24 older female patients with stage III,IV anterior pelvic floor dysfunction were enroled. The individual treatment was formulated according to the wishes of patients, of which 12 patients underwent anterior pelvic organ reconstruction using polypropylene patch (test group), and 12 patients underwent the simply modified vaginal repair treatment (control group). Patients were folowed up for 12 months after repair. The clinical symptom remission, wound healing, pelvic organ prolapse staging and subjective disability index scores were observed. RESULTS AND CONCLUSIONS: After repair, the clinical symptoms in these two groups were significantly aleviated, and there was no infection, poor wound healing and other adverse reactions. At 12 months of folow-up, the subjective disability index scores in these two groups were al significantly lower than those before repair (P < 0.05); moreover, these scores were significantly lower in the test group than the control group (P < 0.05). There were eight cases of pelvic organ prolapse staging I, four cases of pelvic organ prolapse staging II in the test group;seven cases of pelvic organ prolapse staging I, two cases of pelvic organ prolapse staging II, two cases of pelvic organ prolapse staging III and one case of pelvic organ prolapse staging IV in the control group. The degree of organ prolapse in the test group was lower than that in the control group (P < 0.05). These results demonstrate that polypropylene patch repair used in older female anterior pelvic organ reconstruction is safe and effective, and leads to a low recurrence rate.

Cancer stem cells (CSC) play a crucial role in the tumor angiogenesis.Angiogenic factors and hypoxia mediate the process.CSC car differentiate into vascular endothelial cells and participate in the formation of vasculogenic mimicry,which may be one of the key factors in the initiation and progression of tumors.lndepth studies of the roles of CSC in tumor angiogenesis might help provide the basis for developing more effective therapeutic drug target.

The innovation in each aspect of medical and public health territory in our state gradually becomes the social focus.We analyze the key issues in the new type medical innovation in this paper,including clarifying government-dominant leadership of the medical innovation,identifying public welfare property of station-owned hospitals as the direction of medical innovation,impelling public health agency to perform total amount of money budget,and setting everyone's enjoying elementary medical service as the ultimate target of the medical innovation.Meanwhile,some beneficial opinions are also introduced in the paper.

Objective To identify genes associated with prognosis or differentiated type in gastric cancer from fre quently mutated genes or highly-expressed genes,using large-scale genomic data from The Cancer Genome Atlas.Methods The somatic mutation data,RNAseqV2 data and clinical information were downloaded from the TCGA website.The frequency of deleterious somatic mutations for each gene was counted to select the frequently mutated genes.DESeq2 was used to analyze the gene expression data.Then survival analysis was performed on genes highlyexpressed in the tumor tissue.Kaplan-Meier curves were generated by R-survival package,and significance was evaluated by log-rank test.Results The frequency for pathogenic mutations in PIK3CA and APC was significantly discordant between different grades of gastric cancer.2 040 genes were up-regulated in tumor tissue,while 2 357 genes were down-regulated.Among the up-regulated genes,7 genes were associated with poor prognosis of gastric cancer and one was associated with better prognosis.Conclusions Genes associated with differentiation types or prognosis in gastric cancer are identified.The result may clue us future research on potential prognostic markers in clinical treatment.

Objective To understand the status and related influencing factors of critical thinking of medical students, so as to provide recommendations for the training methods of critical thinking of medical students. Methods A total of 380 medical students in third and fourth grade of a medical university were investigated by using the California Critical Thinking Tendency Questionnaire and stratified random sam-pling method. 356 valid questionnaires were recovered. SPSS 22.0 software was used for statistical analysis, including t-test, analysis of variance and multiple linear regression analysis and other methods. Results The Cronbach'sαcoefficient of the scale was 0.728, and the cumulative variance contribution rate was 68.43%. The average score of positive critical thinking ability of medical students was 196.02, showing that they had positive thinking ability. Multiple linear regression analysis showed that the degree of extracurricular read-ing, whether there was a part-time job, and the students' self-study time per week had positive correlation with the critical thinking of medical students (P<0.05). Conclusion The critical thinking ability of medical students needs to be improved and the factors of critical thinking need to be further studied, and the research and practice of cultivating medical students' critical thinking ability must be deepened.

Objective:To establish bacterial endotoxin test for brozopine. Methods: Interference pre-test and interfering factors test were conduced on 3 batches of samples from 2 manufacturers to confirm the applicability of bacterial endotoxin test and the non-in-terfering concentration. The bacterial endotoxin test was carried out based on the method described in the second part of Chinese Phar-macopeia (2010 edition) and relevant standards and guidelines. Results: The three batches of brozopine showed no interference in bacterial endotoxin test at the concentration less than or equal to 2. 5 mg·ml-1 . The bacterial endotoxin test of the three bathes of samples all met the requirements. Conclusion:Bacterial endotoxin test can be used for the quality control of brozopine.

Objective To observe the intervention effect of Schisandrin B (Sch B) on cisplatin induced acute kidney injury (AKI) in mice and its possible mechanism.Methods Twenty-five BALB/c mice were randomly divided into blank control group, model group, low and high dose of Sch B intervention groups and Sch B control group. Olive oil with Sch B was administered by gavage at the dose of 20 mg/kg or 100 mg/kg for low and high dose of Sch B intervention groups respectively; olive oil with Sch B 100 mg/kg was applied by gavage to the Sch B control group; the same volume of olive oil was perfused into the gastric cavity in the blank control group and model group; the above measures in various groups were consecutively used for 5 days. On the 3rd day of the experiment, AKI mice model was established by intraperitoneal injection of cisplatin (20 mg/kg) once and the same measure was given to the low and high dose of Sch B intervention groups; 1 mL/kg normal saline was injected into the peritoneal cavity in the bland control group and Sch B control group. At the end of the experiment, the serum creatinine (SCr) level was determined; apoptosis of renal tubular epithelial cells were detected by using terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) assay; the morphological changes of renal tubular epithelial cells were observed by hematoxylin eosin (HE) staining, and renal tubular injury score was evaluated; p53 protein content in the kidney tissue was measured by immunohistochemical analysis; furthermore, expressional level of p53 protein in renal tissue was tested by Western Blot.Results Compared with the blank control group, the level of SCr (μmol/L: 86.77±10.97 vs. 14.37±0.81), renal tubular injury score (9.67±1.20 vs. 1.00±0.45), the count of apoptotic renal tubular epithelial cells (cells/200 power field: 20.00±2.13 vs. 2.30±0.40) in the model group were all increased (P < 0.05 orP < 0.01), and p53 protein content (cells/400 power field: 13.40±2.66 vs. 57.30±3.82), and the expression of p53 protein [absorbency (A value) ratio: 0.79±0.09 vs. 1.42±0.09] in model group were decreased (bothP < 0.01). Compared with the model group, in the low and high dose Sch B intervented groups, the level of SCr (μmol/L: 21.98±5.52 and 37.45±5.04), renal tubular injury score (5.67±0.76 and 6.17±0.65), the count of apoptotic renal tubular epithelial cells (cells/200 power field: 10.60±1.05 and 11.60±1.45) were all reduced (allP < 0.01), p53 protein content (cells/400 power field: 42.40±3.67 and 45.90±2.31) and the expression of p53 protein (A value ratio: 1.36±0.16 and 1.25±0.11) were increased (bothP < 0.01). HE staining showed the pathological changes of renal tubules, such as renal tubular epithelial cellular fusion, vacuolization, cast formation, and tubular lumen constriction/dilation in model group; the pathological changes in kidney tissues observed in low and high dose Sch B intervention groups were milder than those in model group.Conclusion Sch B plays a beneficial role in the cisplatin induced AKI in mice, and its protective effect might be mediated by decreasing SCr, regulating p53 protein expression level and inhibiting the apoptosis of renal tubular epithelial cells.

Objective: To study optimum inclusion process conditions for Bencaosuansu. Methods: The study was carried out with orthogonal design. The process conditions were studied by determining the utilization ratio of Bencaosuansu, the oil-bearing rate and extract ratio of inclusion compound. Results: The optimum preparation conditions for inclusion were established as: Bencaosuansu: ?-CD was 1∶12, pH6, the inclusion time was for 5h. The utilization ratio of Bencaosuansu is 94.61 percent. Conclusion: The method can be used for production of ?-CD inclusion compound.

Objective To explore the relationship between body mass index (BMI) and frailty among community aged people. Methods A total of 1571 aged people in two communities in Zhengzhou, Henan, China were recruited by convenience sampling method. They were divided into normal group, overweight group and obesity group according to BMI, and were assessed with Fried Frailty Criteria. Results The rates of overweight and obesity were 38.6%and 10.9%, respectively. There was significant difference in the level of frailty among dif-ferent groups (χ2=86.659, P<0.001). Multivariate Logistic regression analysis showed that overweight and obesity were independent risk fac-tors of pre-frailty (P<0.05), and obesity was the independent risk factor of frailty (P<0.05). Conclusion The prevalence of overweight and obesity was higher in community aged people in Zhengzhou, and normal BMI was important to postpone the process of frailty.