Objective To survey the incidence of HIV/HCV co-infection and its epidemiological characteristics among drug addicts in Urumqi City.Methods From June to November,2007,1000 blood samples were collected sequentially from the drug addicts,who were admitted to Urumqi City Detoxification Center,for HIV and HCV detection by ELISA.The tests for screening,reexamination and confirmation were carried out under the guidance of the instruction for the use reagent rigorously,and the results were assessed according to corresponding technical regulation issued by authorities.The surveillance registration tables of drug addicts were filled for statistical study and analysis.Results The infection rates of HIV and HCV among 1000 drug addicts were 26.3% and 72.3% respectively.The co-infection rate of HIV/HCV was 25.5%.96.9% of HIV positive addicts were co-infected with HCV.There were significant differences in the infection rates of HIV,HCV and HIV/HCV co-infection between intravenous drug users and non-intravenous drug users.There were significant differences in the infection rates of HIV,HCV and HIV/HCV co-infection between Uygur and drug addicts of other ethnics.Conclusions A high co-infection rate of HIV/HCV exists in the drug addicts of Urumqi City,and even worse is that most HIV positives have co-infected with HCV.The infection rates of HIV,HCV and HIV/HCV co-infection in intravenous drug users are obviously higher than that in non-intravenous drug users.The infection rates of HIV,HCV and HIV/HCV co-infection in Uygur drug addicts are higher than that in other ethnics.

Objective To investigate the clinical effects of preoperative eye position training on femtosecond laser-assisted laser in situ keratomileusis(FL-LASIK)? Methods One hundred and sixty-eight myopia patients(328 eyes)scheduled for selective FL-LASIK were randomly divided into the experiment group of 85 cases(166 eyes)and the control group of 83 cases(162 eyes)?The patients in the control group were given the routine preoperative education and the patients in the experiment group received the preoperative intervention of eye position besides routine preoperative education? The two groups were compared in terms of intraoperative changes of eye position,corneal topography and the postoperative visual acuity? Results The frequency of eye position adjustment and machine stopping in the experiment group was significantly smaller than that of the control group(P < 0?001)? But no significant differences were found between the two groups in corneal topography and the postoperative visual acuity(P > 0?05)? Conclusion Preoperative training of eye position may maintain ideal eye position and reduce the frequency of downtime due to eye position adjustment during FL-LASIK,which may ensure the successful completion of FL-LASIK?

Objective To understand cognition status about AIDS prevention knowledge of urban and rural residents in Xinjiang Yili state ,and to provide a scientific basis for formulating measures of AIDS publicity ,education ,prevention and control for the fu-ture .Methods For simple random sampling with Database of country infectious diseases special projectsthat Yili state has been successfully uploaded ,obtain the required sample ,then conducted questionnaire survey .Results In 1 101 respondents ,cognition rate about AIDS prevention knowledge of urban and rural residents was 79 .02% ,the higher level regions of AIDS epidemic classifi-cation ,respondent in the higher cognition rate ;the cognition status about blood transmission ,mother-to-child transmission was bet-ter ,the sexual transmission was poorer .Male ,16 to 30 years old ,Uygur ,the higher education level of respondents ,AIDS prevention knowledge in relatively good condition status .Conclusion The cognition status about AIDS prevention knowledge in Xinjiang Yili state basic meet the national standards ,but remains to be improved .We should enhance AIDS prevention knowledge education cope with high age group ,Kazakhs and other ethnic minorities ,low education level of the respondents ,it should also focus on strengthe-ning the publicity education and proper guidance with non-transmission .According to different popular level classification ,publicity and education should be carryed out combined with local actual formulation is more advantageous to measure .

Objective To investigate the clinical liver function protective effect of Shuganning injection for treatment of patients with drug poisoning.Methods One hundred and forty patients with drug poisoning consistent with thecriteria of enrollment into the study were admitted into the Department of Emergency Medicine in the First Hospital of Jilin University-the Eastern Division from January 2015 to August 2016, and they were randomly divided into observation group and control group by the computer generated random numbers, 70 cases in each group. After admission, all the cases were treated with routine treatment including detoxification, removal of toxin, organ protection, symptomatic and supporting treatment, etc. Based on the routine treatment, additionally, Shuganning injection 20 mL+ 10% glucose injection 250 mL, intravenous drip slowly, once a day was given in the observation group; the therapeutic course was 14 days in both groups. The changes of serum glutamic transaminase (ALT), aspartate aminotransferase (AST) and total bilirubin (TBiL) levels were determined before and 7 and 14 days after treatment respectively to evaluate the situation of liver function in the two groups.Results Before treatment, there were no statistical significant differences in the levels of serum ALT, AST and TBil in the two groups (allP > 0.05); with the prolongation of treatment, the above-mentioned indexes of the two groups were gradually reduced, on the 14th day after treatment, they reached the lowest levels, and the degree of decrease in level on the 14th day in the observation group was more significant than that in the control group [ALT (U/L): 32.6±10.8 vs. 98.3±34.5, AST (U/L): 39.4±14.3 vs. 138.5±25.6, TBil (μmol/L): 4.8±1.7 vs. 13.2±2.3, allP < 0.05].Conclusion Shuganning injection has a protective effect on liver function in patients with drug poisoning.

Objective To investigate the effects of Danshen Duofensuanyan on the blood coagulation,inflammatory and cardiac function in acute myocardial infarction(AMI) patients who did not accept emergency percutaneous coronary intervention.Methods One hundred and twenty patients,from January 2012 to November 2014 in the First Hospital of Qinhuangdao admission due to acute myocardial infarction frequent episodes of angina(Killip grade Ⅱ,grade m),were randomly divided into two groups:control group (n =60) and observation group (n =60).All patients suffered from severe angina after AMI and heart failure but without PCI.All included patients were gave antiplatelet drug,anticoagulants and Statins,etc.And the patients in observation group were additionally given 7-day Danshen Duofensuanyan injection 200 mg/d once daily intravenously.Platelet aggregation rate (PAG),fibrinogen (FIB),c-reaction protein (CRP),erythrocyte sedimentation rate (ESR),troponin Ⅰ (TNI),plasma brain natriuretic peptide (BNP) and left ventricular end diastolic dimension(LVEDD),left ventricular ejection fraction (LVEF) and cardiac output (CO) of two group were monitored before and after treatment.Results (1)Changes in coagulation,inflammatory cytokines and enzymes between the treatment group and the control group after treatment (Treatment Group:PAG (22.32 ±17.61) ％,FIB(3.58±0.74) g/L,CRP(9.34±1.82) mg/L,ESR(10.1±4.6) mm/1 h,TNI(4.51± 1.89) ng/ml);control group:PAG(31.32±21.62)％,FIB(3.84±0.62) g/L,CRP (14.32±1.79) mg/L,ESR(13.2 ± ±5.4) mm/1 h,TNI(5.32±2.31) μg/L) and before treatment(Treatment group:PAG(88.87± 18.21) ％,FIB (4.78±0.97) g/L,CRP(32.13±11.59) mg/L,ESR(28.5±6.1) mm/1 h,TNI (56.43 ± 21.87) μg/L);control group:PAG (89.53± 19.35) ％,FIB (4.66 ± 0.78) g/L,CRP (29.06± 12.47) mg/L,ESR (29.3 ± 3.2) mm/1 h,TNI (53.69± 18.76) μg/L) were significandy improved,and the differences were statistically significant (P＜0.01),and observation group had more significant improvements than the control group(P＜0.05 or P ＜0.01) after treatment.(2) All aboved indexes were improved after treatment except LVEDD.And Danshen Duofensuanyan can significantly improve PAG,FIB,CRP,ESR,TNI,BNP LVEF and CO(P＜0.05 or P ＜0.01).Conclusion Danshen Duofensuanyan can effectively improve the blood rheology and the frequency of angina,and then improve heart functions.

Objective To explore the roles and functions of the community nurses in community health service centers and their main work content in Xi′an. Methods A total of 320 community nurses in Xi′an were investigated by General Information Questionnaire and revision of Community Nurses′Roles and Functions Questionnaire. Results The work often undertaken by community nurses were: health education, community prevention, providing direct care and chronic disease management, however, the hospice care, care for people with mental disorders, family planning guidance and rehabilitation nursing were seldom involved. The scores of each role from high to low were as follows:the organizers and managers, coordinators and partners, health educators, caregivers, observer and researcher and spokesman with(2.56±0.99),(2.43±0.97), (2.38±0.84),(2.34±0.87),(2.29±0.10),(2.07±0.99) points. Conclusions The job of community nurses in Xi′an includes the medical service, disease prevention, healthcare, rehabilitation and health education etc. However, the well done jobs only are limited to some of simple functions, such as health education and health guidance are done well. The work of hospice care and mental care which need more professional abilities and interpersonal cooperation need to be promoted.

Objective To determine the plasma and intracellular concentration of Ara-C by the RPHPLC method and analyse the influence factors and the relationship between the concentration and drug dose.Methods Mononuclear cells and serum of 75 patients with acute leukemia were extracted after the first intravenous infusion of different administration dosage of Ara-C (0.5, 1.0, 2.0 g/m2), and analysed with different chromatographic conditions by RP-HPLC. Results The linear range of Ara-CTP was 0.28-18.96 μg/ml (r =0.998), and the detection limit was 0.28 μg/ml. The detection limit of Ara-C and Ara-U in plasma was 0.0157 μg/ml and 1.034 μg/mnl respectively. In 27 samples preserved for more than 1.5 years, 11 (40.7 %)cases of the plasma concentration of Ara-C were below the detection limit. In 36 samples of mononuclear cell count below 1.5×106/ml, 15 cases (41.7 %) of intracellular concentration of Ara-CTP were below the detection limit. The plasma concentration of Ara-U and intracellular concentration of Ara-CTP were increased with administration dosage of Ara-C increased, and the plasma concentration of Ara-C was not increased. The intracellular concentration of Ara-CTP in old patients over 40 years was tend to in crease with age.Conclusion The RP-HPLC method is simple, rapid, stable, reproducible and applicable for the monitoring of the plasma concentration of Ara-C and intracellular concentration of Ara-CTP. In 0.5-2.0 g/m2 dose range of Ara-C, the plasma concentration of Ara-U and intracellular concentration of Ara-CTP was increased with administration dosage of Ara-C increased.

Objective:To observe the recent effects and toxicity of thermochemotherapy on malignant hydrothorax or hydroperitoneum,to evaluate the changes of the immunological functions,and to investigate the mechanism of thermochemotherapy.Methods:Fifty-two patients were treated with weekly intracavitary chemotherapy,and then combined with local endogenetic thermotherapy twice a week.As the control,another 50 patients received weekly intracavitary chemotherapy.The treatment lasted for two weeks and was followed by one-week rest,and then the recent effects and toxicity were observed.The T cell subset,NK cells and VEGF levels in serum,hydrothorax or hydroperitoneum were tested.Results:Overall response rates of the malignant hydrothorax were 86.9% vs 60.0%(P

Objective:Position emission tomography/computed tomography(PET/CT) is a new bio-imaging system which is combined metabolic with anatomic imaging.This study was to compare the accuracy of conventional staging methods(including computed tomography,ultrasound,magnetic resonance imaging,and detection of bone marrow) with that of PET/CT for lymphoma staging and re-staging. Methods:A total of 42 patients with lymphoma diagnosed by operation or biopsy,received conventional and PET/CT staging.The accuracy of these two methods and their impact on lymphoma staging were compared.Results:The accuracy of PET/CT scan was 95.2%(40/42),and that of conventional staging was 78.6%(33/42).The detection rates of internal lymph node were 97.1%(66/68) and 88.2%(60/68),respectively.The detection rates of outer lymph node were 91.7%(22/24) and 58.3%(14/24),respectively.Compared with conventional staging methods,7 cases were up-staged and 2 cases were down-staged by PET/CT,which led to the change of therapy in 8 cases.Conclusion:PET/CT scan is more sensitive and accurate than conventional staging methods in staging and restaging of lymphoma.

Objective:To evaluate the biological characters of C57-TgN(HBV adr2.0)SMMU transgenic mice. Methods: Integration,expression,replication and histology change of hepatitis B virus gene in F6 transgenic mice were estimated by ge-nomic DNA PCR,Western blotting,ELISA,immunohistochemistry,serum DNA PCR,transmission electron microscopy and H-E staining. Results: Hepatitis B virus gene was integrated into F6 C57-TgN(HBV adr2. 0)SMMU transgenic mice and expressed HBsAg,HBcAg and X protein in liver tissue. HBsAg and HBeAg were expressed in serum of 19. 54% and 3. 39% F6 transgenic mice. Hepatitis B virus were replicated in serum and liver tissue of transgenic mice. Long-term integration,expression and replication of hepatitis B virus gene induced pathological lesion of transgenic mice liver and lung. Conclusion: C57-TgNCHBV adr2. 0)SMMU transgenic mice line has the biological characters including integration of hepatitis B virus gene into genomic DNA,expression and replication of hepatitis B virus gene in serum and liver, and histological change in liver and lung. It is a valuable animal system to study pathogenesis, treatment and prevention of hepatitis B virus.