Objective:To study the efficacy comparison between the antibacterial catheter and common central venous catheter in ICU patients.Methods: The 110 ICU patients with central venous catheter were randomized into the control group (common central venous catheter)and the observation group (antibacterial catheter), 55 patients per group. The success rate of catheter, the averange catheter days, complications, and the incidence of catheter-related infection (CRI)and pathogen distribution in the two groups were observed and compared.Results: Compared with the control group, the success rate of catheter was significantly increased(100.0% vs 81.8%,x2=11.00,P<0.05), the averange catheter days were obviously reduced(12.5±3.0 vs 13.5±3.0,t=3.49,P<0.05)days, the incidences of complications (red and swelling in puncture site(3.6% vs 27.3%,x2=11.76,P<0.05), fluid in puncutre site (3.6% vs 21.8%,x2=8.18,P<0.05), catheter herniation(1.8% vs 20.0%,x2=9.35,P<0.05)were significantly decreased in the observation group with statistical difference (P<0.05). Conclusion: The antibacterial catheter can significantly reduce the incidence of CRI for ICU patients and improve the security of central venous catheter.

Objective To investigate the role of integrin CD11b in liver ischemia/reperfusion (I/R) injury and its possible mechanism.Method CD11b-/-and WT (C57BL/6) mice were used to establish a 70％ liver warm I/R by clamping the left and median liver lobes for 60 min with vascular micro clamp at 37℃,then the clamp was removed and the abdominal incision was sutured.The blood plasma and liver samples were obtained at different time points (1,3,6,12,24 and 48 h) postreperfusion to assess liver function and cellular injury.Serum ALT and AST levels were determined,and HE staining and TUNEL assay were performed to estimate the severity of liver damage.Tumor necrosis factor-α (TNF-α) and interleukin-10 (IL-10) were assayed by Reverse transcription polymerase chain reaction (RT-PCR).Kupffer cells were isolated from the live,and the reduced form of nicotinamide-adenine dinucleotide phosphate (NADPH) oxidase activity and active oxygen species (ROS) production were assayed.Result CD11b-/-mice displayed a significantly preserved liver function as represented by lower alanine aminotransferase (ALT) and aspartic transaminase (AST) levels,less histological damage and apoptosis compared to WT mice.Furthermore,TNF-α was decreased and IL-10 mRNA expression was increased in CD11b-/-mice compared to WT mice.Finally,CD11b-/-mice showed decreased activity of NADPH oxidase and less ROS production.Conclusion Integrin CD11 b may regulate the levels of inflammatory (TNF-α) and anti-inflammatory (IL-10) cytokines,enhance the activity of NADPH oxidase in Kupffer cells and enrich the production of ROS,which aggravate liver I/R injury.

Objective To observe the influence of bezafibrate on the apoptosis and expression of lectin-like oxidized low density lipoprotein receptor-1(LOX-1) mediated by oxidized low density lipoprotein(ox-LDL) in cultured human umbilical vein endothelial cells(HUVECs).Methodes The apoptosis of HUVECs mediated by ox-LDL were evaluated by flow cytometry and the expression of LOX-1 mRNA were detected by RT-PCR.Results Compared with the control group,ox-LDL could increase apoptosis and the expression of LOX-1(P<0.05),and Bezafibrate could decrease the apoptosis and the expression of LOX-1 in a concentration -dependent manner(P<0.05).Preincubation of HUVECs with polyinosinic acid for 2 hours,the apoptosis and the expression of LOX-1 decreased(P<0.05).Conclusions Bezafibrate inhibits the apoptosis of HUVECs mediated by ox-LDL by reducing the expression of LOX-1,which may be part of the reasons for bezafibrate to prevent and treat atherosclerosis.