Objective To investigate the CT angiography (CTA) in the diagnosis of intracranial atherosclerosis.Methods Sixty-eight cases of suspected ischemic cerebrovascular disease patients were enrolled in this study,and all patients underwent CTA.Parallel image post-processing was done.CTA characteristics were observed and compared with digital subtraction angiography (DSA) results.Results A total of 376 lesions in 68 cases were examined,112 lesions were found in CTA; intracranial atherosclerotic plaques mainly distributed in the internal carotid artery (53 lesions),anterior cerebral,middle and posterior artery (39 lesions) and vertebral-basilar artery (20 lesions).In narrow artery segment,stenosis with calcified plaque was in 32 lesions,mostly in the middle cerebral artery,the cavernous segment of the internal carotid artery,clinoids segment and intracranial vertebral artery.For non-calcified plaque,CTA showed fuzzy.DSA showed 128 total lesions,and severe stenosis rate in CTA [9.31％ (35/376)] and DSA [9.31％ (35/376)] was consistent.Thirty-eight patients were correctly diagnosed.Conclusions CTA as a minimally invasive intracranial atherosclerosis diagnostic methods,consumes less time with low cost and high spatial resolution.It is suitable for clinical universal application.

Objective To investigate serum metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) in non-small cell lung cancer (NSCLC) and observe gefitinib drug sensitivity before and after transfecting MALAT1-siRNA in epidermal growth factor receptor ( EGFR ) wild-type cell line A549.Discussing the relationship between MALAT 1 and epidermal growth factor receptor-tyrosine kinase inhibitor ( EGFR-TKI) drug resistance.Methods Serum MALAT1 was quantified by qRT-PCR in 90 cases of NSCLC, 30 cases of lung benign tumor and 30 cases of healthy people in the First Affiliated Hospital of Dalian Medical University during April to September in 2015.MALAT1 expression in cancer cells were detected in the cell lines A549, H1299, 95D and HCC827.The half maximal inhibitory concentration ( IC50) of gefitinib in cells before and after transfecting MALAT 1-siRNA were analyzed by cell proliferation toxicity test ( CCK).Flow cytometry technology was used to evaluate the apoptosis rates.One-way analysis of variance was statisticed between groups.Results Serum MALAT1 expression was increased in NSCLC compared with healthy control group ( NSCLC =1.88 ±2.51, Healthy control group =1, t=3.347, P<0.01).MALAT1 was over expressed in the EGFR wild-type cell lines compared with the EGFR mutant cell lines(HCC827=1, A549=4.44 ±1.05, 95D=3.55 ±0.63,H1299 =1.44 ±0.05).The IC50 value of gefitinib was declined 32.1% by silencing MALAT1.The results of flow cytometry showed an increasing apoptosis rate after silencing MALAT1 in A549 cells[NC=(9.20 ±1.85 )%, M=(10.24 ±3.43)%, NC+G=( 14.57 ±0.41 )%, M +G =( 21.48 ±2.47 )%].Conclusions Serum MALAT1 reflected the progression of NSCLC.Silencing MALAT1 resulted in enhancement in sensitivity to gefitinib in A549 cell line.It prompted the partly reversal of gefitinib resistance by MALAT1.

Objective To evaluate the clinical value of percutaneous catheter drainage under the guidance of ultrasound in the treatment of hepatic abscess.Method Forty-eight cases who were clinical diagnosis of liver abscess by percutaneous catheter drainage was selected as the observation group.There were 12 cases of liver abscess diagnosed by parallel surgical drainage in the treatment as the control group.To observe the clinical effect,hospitalization time and complications of two groups of patients after treatment.Results The cure rate of observation group was significandy higher than that in control group:100％ (48/48) vs.The temperature recovery time and hospitalization time of the observation group were significantly shorter than those in the control group:(1.5 ±0.3) d vs.(3.2 ±0.8) d,(9.3 ±2.3) d vs.(17.5 ±3.2) d.No complications occurred in the observation group,and there were 2 cases in the control group.Conclusions Percutaneous catheter drainage in the treatment of liver abscess has advantages of simple economy,less trauma,less complications and high cure rate.It has become the first choice for treatment of liver abscess.

AIM:To study the effect of Taxol on the proliferation, apoptosis, and mRNA expressions of α2,6-sialic acid (SA) and α2,6-sialyltransferase (ST6Gal) in mouse cervical cancer cell line U14.METHODS:After the U14 cells were treated with Taxol, the IC50 value of Taxol to U14 cells was detected by MTT assay.The expression of α2,6-SA and apoptosis-related factors (Bcl-2, Bax, caspase 8 and caspase 3), the apoptosis rate and cell cycle were determined by flow cytometry.The mRNA expression of ST6Gal1 and ST6Gal2 was detected by qPCR.RESULTS:As compared with control group, Taxol induced obvious U14 cell growth inhibition, reduced α2,6-SA expression, up-regulated Bax, down-regulated Bcl-2, decreased the ratio of Bcl-2/Bax, enhanced caspase 8 and caspase 3 activity, increased the apoptotic rate and cell proportions of Sub-G1 and S phases, and induced G2/M phase arrest.Taxol also down-regulated the mRNA expression of ST6Gal1, and slightly up-regulated the mRNA expression of ST6Gal2.CONCLUSION:α2,6-SA and ST6Gal are involved in the multiple effects of Taxol on modulation of the cell cycle and apoptosis in U14 cells.

Influences of environment on medical equipment lie in many fields and exist independent of human consciousness.In order to lengthen the service life of medical equipment and produce the best possible results,human beings use scientific methods to utilize and change the natural situation.Bad influence from natural conditions to medical equipment is expatiated from such aspects as influence from thunder,rain,snow,electricity,warmth,humidity,mist,magnetism,etc.The management and the protection of the medical equipment under the natural situations are discussed.

Objective To investigate the relationship between self-esteem and social anxiety,and the role of putting into perspective played in this relationship in university students.Methods Sample consisted university students of 376 females and 164 males,data were obtained on cognitive emotion regulation questionnaire,social anxiety subscale of the self-consciousness scale,and self-esteem scale.Results Cognitive emotion regulation strategies of putting into perspective is significantly and positively related to self-esteem(r=0.12,P＜ 0.01),it was also significantly and negatively related to social anxiety(r =-0.01,P ＜ 0.05) ; self-esteem was significantly and negatively related to social anxiety(r =-0.35,P ＜ 0.001).Cognitive emotion regulation strategies of putting into perspective played both a moderating(AR2 =0.124,P＜0.05) and mediating role(F=30.717,P＜ 0.01) in the relationship between self-esteem and social anxiety.Conclusion Self-esteem is not only directly but also indirectly associated with social anxiety because of the moderation and mediation model of putting into perspective.

Objective To study the chemical constituents of the whole plant of Pholidota yunnanensis and to examine the inhibitory effects of the isolated compounds on nitric oxide(NO) production in activated rat macrophage-like cell line(RAW 264.7).Methods Various chromatographic techniques were used to separate and purify the constituents,and their structures were elucidated on the basis of spectroscopic analysis. The inhibitory effects of these compounds on NO production in RAW 264.7 cells of rats activated by lipopolysaccharide(LPS) and interferon-?(IFN-?) were tested in vitro by microplate ultraviolet-colorimetric method.Results Seven compounds were isolated and identified as trans-3,3′,5-trihydroxy-2′-methoxystilbene(Ⅰ),cis-3,3′-dihydroxy-5-methoxystilbene(Ⅱ),trans-3,3′, 5-trihydroxystilbene(Ⅲ),3,3′-dihydroxy-5-methoxybibenzyl(batatasin-Ⅲ,Ⅳ),3,4′-dihydroxy-3′,5-dimethoxybibenzyl(gigantol,Ⅴ),3,3′,5-trihydroxybibenzyl(Ⅵ),2,7-dihydroxy-4-methoxy-9,10-dihydrophenanthrene(coelonin,Ⅶ).Conclusion Compounds Ⅱ,Ⅳ,and Ⅵ show favorable inhibitory effects on NO production,compounds Ⅰ and Ⅱ are new compounds,and compounds Ⅲ-Ⅶ are isolated from this genus for the first time.

Objective To investigate the effect of combined prism lenses for the treatment of juvenile myopes. Methods 622 cases of juvenile myopes ranging from - 1. 0D to - 5. 0D, aging from 6 - 18ages, were divided randomly into two groups. The control group(320 cases) wear general glass and the research group(302 cases) wear combined prism lenses. The juvenile myopes of both groups were checked their vision every 6 months and data were collected at the end of the fourth 6 months and analyzed by SPSS software. Results Ther eyes of increasing diopter in research group were 38(6.3%of all eyes) ,33 eyes were less than 1. 0D and 5 eyes were more than 1. 0D among them;but 294(46.0% of all eyes) in the control,201 eyes were less than 1.0D and 93 eyes were more than 1.0D among them. There were significant differences between two groups(P＜0.01). Conclusion Combined prism lenses for juvenile myopes (lower and middle) .compared with the common glass, had good clinical effect and it was a reliable and practicable method,to wear combined prism lenses for the treatment of juvenile myopes.

Objective To study the effect of glucosides of cbaenomeles speciosa (GCS) on the expression of EP2 and EP4 in synovial tissues from rats with adjuvant arthritis. Methods Complete Freund's adjuvant (FCA) was used to induce adjuvant arthritis (AA) in rats. The synoviocytes from AA rats were collected and incubated. Synoviocytes proliferation was determined by 3-(4,5-dimethylthiazal-2yl) 2,5-dipbeny+hetrazoliumbromide (MTT) assay. Levels of prostaglandins E2(PGE2) and cAMP were determined by radioim-munoassay (RIA). The expression of EP2 and EP4 was detected by Western blot analysis. Results GCS significantly inhibited the proliferation of synoviocytes. GCS decreased the level of PGE2 and increased the level of cAMP. The results of correlation analysis demonstrated that GCS inhibited synoviocytes hyperplasia by increasing the level of cAMP in synoviocytes. The results of Western blot analysis demonstrated that GCS significantly improved the expression of EP2 and EP4. Condusion These results suggest that restoring normal transduction of the EP-G protein-cAMP signal pathway may be one of the most important mechanisms of GCS.

Objeetive To investigate the expression of claudin-4 in the eutopic and ectopic endometfium of women with endometriosis and evaluate the role of clandin-4 in the pathogenesis of endometriosis.Methods Thiny-five women with endometriosis and 35 controls were studied.Expression of elaudin-4 was investigated using immunohistochemistry,western blot and RT.PCR,respectively.Morphologic change of tight junction was also observed in different kinds of endometria.Results (1)G1andular epithelial cells of control endometrium and eutopic endometrium showed intact tight iunctions in electron micrographs,whereas the morphology of tight junctions in ovarian endometriotic tissue was disrupted and collagen bundles could be easily detected.(2)The immunohistochemical staining of claudin-4 was localized to the glandular epithelial cell membrane.Deftcient or weak staining Wag found in ovarian endometriotic tissues. In control endometrium.eutopic and ectopic endometrium of women with endometriosis,the expression of clandin-4 protein Was 89±24.84±22 and 27±14.respectively.Relative expression of claudin-4 mRNA Was 14.5±6.8,13.8±9.5 and 2.6±2.5.respectively.Expression of claudin-4 Was significantly lower in the ectopic endometriotic tissue than in the eutopic cndometrium and the control at both mRNA and protein levels(P<0.05). N0 significant difference Was foand between eutopic endometrium from women with endometriosis and control endometrium from women without endometriosis (P>0.05).Conclusion Down-regulated expression of claudin-4 might play a pathogenic role in the formation of endometriosis.