1.Antihyperglycemic Activity of Herba Taraxaci Polysaccharides
China Pharmacy 2007;0(27):-
OBJECTIVE:To study the antihyperglycemic activity of Herba Taraxaci polysaccharides.METHODS:1,1-diphenyl-2-picryl-2-picrylhydrazyl(DPPH) radical scavenging and FRAP assays were used to analyze the antioxidant activity of Herba Taraxaci polysaccharides;the inhibitory activity of ?-glucosidase in vitro was tested by micromethod; the diabetic mice model was established using alloxan. After intragastric administration of Herba Taraxaci polysaccharide for 14 days,the fasting serum glucose and antioxidant parameters (SOD,MDA and GSH-Px) in dissected liver tissue were assayed. RESULTS:The TEAC values assayed by DPPH and FRAP methods were 564.98 ?molTE?g-1 and 1 399.55 ?molTE?g-1,respectively; and the IC50 value of Herba Taraxaci polysaccharides against ?-glucosidase was 49.27 ?g?mL-1.Herba Taraxaci polysaccharides had no effect on serum polysaccharides and antioxidant parameters in normal mice,but which significantly down-regulated the fasting serum glucose of diabetic mice especially at a high dosage(P
2.Study on immunosensor of immobilized HRP-HBV antibody on Au colloid
Liu QIN ; Zhongming LIU ; Xiaoyong ZOU
Chinese Medical Equipment Journal 1993;0(06):-
Objective To form Hepatitis B electrochemical immunosensor for the clinic detection. Methods Using the gold electrode as the fundus,the thionine and the HBV horseradish peroxidase(HRP) antibody were electrostaticly bounded to the self-assemble monolayer competitively to form the Hepatitis B electrochemical immunosensor. Cyclic voltammetry and linear scan voltammetry were used to investigate the chemical characteristics of the immunosensor. Results The linearity of Hepatitis B in the range of 1:30~1:200 with the correlation coefficient of 0. 994 was obtained. Conclusion The Hepatitis B electrochemical immunosensor formed by this way can be used to the clinic detection.
3.Study on clinical effect of Novonorm on treating type 2 diabetic patients with atherosclerosis
Hanbei CHEN ; Yi LIU ; Xiaoyong LI
Clinical Medicine of China 2010;26(11):1187-1189
Objective To assess the clinical effect of Novonorm on treating type 2 diabetic patients with atherosclerosis. Methods Sixty-five patients of type 2 diabetes with atherosclerosis were randomly divided into 2 groups :36 patients took Novonorm,and the other 29 individuals were treated with Glipizide. The blood glucose and CIMT were compared before and after treatment for 12 months. Results The postprandial blood glucose decreased by(1.99 ± 1.06)mmol/L after the treatment of Novonorm,which was significantly higher than that of(0. 99 ±0. 54)mmol/L after the treatment of Glipizide(P < 0. 05). CIMT significantly decreased from (1.11 ± 0. 07)mm before treatment to(1.02 ± 0. 08)mm after treatment in the Novonorm group(P < 0. 05),whereas CIMT were similar between after and before treatment in the Glipizide group(1.07 ± 0. 06)mm vs.(1.10 ± 0. 08)mm(P > 0. 05).Conclusions Novonorm is a kind of effective and safe regent,which can be used to treat type 2 diabetic patients with atherosclerosis.
4.The clinical significance of locating percutaneous puncturing sites and track according to the spinous process
Xiaoyong LIU ; Huilin YANG ; Tiansi TANG
Chinese Journal of Orthopaedics 2001;0(08):-
0.05). Conclusion The study of the characteristic of track of percutaneous transpedicular puncture is good for the safe procdure under image guidance. The standard track is safe for transpedicular or parapedicular puncture. The percutaneous transpedicular puncturing sites can be located according to the spinous process.
5.Intravascular stent implantation in treating patients with symptomatic vertebral artery kinking
Xiaoyong LIU ; Zhenhua WANG ; Pengfei HOU
Chinese Journal of Postgraduates of Medicine 2016;39(8):734-737
Objective To evaluate the feasibility and safety of stent implantation in treating patients with symptomatic vertebral artery kinking. Methods Forty-two patients with vertebral artery kinking confirmed by digital subtraction angiography and accorded with the stent implantation indications were chosen in our study; these patients were performed stent implantation. The stenosis and haemodynamics changes, and recent and postoperative complications were observed; Malek scale was adopted to evaluate the clinical efficacy during the 1-year follow-up. Results The success rate of stent implantation for patients with symptomatic vertebral artery kinking was 100%(42/42). The percentage of stenosis was significantly reduced from (75.6 ± 13.5)%to (8.3 ± 3.1)%after stenting, and the mean flow velocity of kinking lesion was obviously decreased from (49.5 ± 12.4) cm/s to (31.7 ± 15.0) cm/s, and there were statistical differences (P<0.01). The cerebellum hematoma was noted in 1 patient , subcutaneous hematoma of puncture point appeared in 2 and femoral artery pseudoaneurysm after the stenting in 1;all these complications did not cause serious consequences. Malek scale indicated that 36 cases got 1 point,4 cases got 2 points, 1 case got 3 points, 1 case got 4 points and no one got 5 points;36 patients (85.7%, 36/42) were asymptomatic during the 1-year follow-up;no significant in-stent restenosis was found. Conclusions Symptomatic vertebral artery kinking can be treated safely and effectively with stent implantation.
6.Changes and clinical significance of serum and cerebrospinal fluid NSE and MBP in patients with craniocerebral injury
Yuyan LI ; Linlin LIANG ; Xiaoyong LIU
International Journal of Laboratory Medicine 2017;38(10):1338-1340
Objective To study the relationship between serum and cerebrospinal fluid neuron specific enolase (NSE) and myelin basic protein (MBP) concentrations with the disease progress and prognosis in the patients with craniocerebral injury.Methods Forty-five patients with craniocerebral injury treated in our hospital were selected and divided into the mild group,moderate group and severe group according to disease severity;which were divided into the subdural hematoma group,epidural hematoma group,cerebral contusion and laceration group and combined injury group according to the injury types;which were divided into the death and plant survival group,disability group and good recovery group.Other 15 individuals undergoing physical examination were selected as the control group.The concentrations of cerebrospinal fluid and serum NSE and MBP were detected at admission in the patients with craniocerebral injury and control group,on 1,7,14 d after injury in the patients with severe craniocerebral injury.Results The concentrations of cerebrospinal fluid and serum NSE and MBP in the patients with mild,moderate and severe craniocerebral injury were significantly higher than those in the control group,the severe group was significantly higher than the moderate group(P<0.05);the concentrations of cerebrospinal fluid and serum NSE and MBP in the patients with epidural hematoma were lowest,while which in the combine injury group were significantly higher than those in the subdural hematoma group,epidural hematoma group and cerebral contusion and laceration group(P<0.05);which in the death and plant survival group were significantly higher than those in the disability group and good recovery group(P<0.05);which on 1-14 d after injury in the patients with severe craniocerebral injury showed the decreasing trend,but which on 14 d were significantly higher than those in the control group(P<0.05).Conclusion The concentrations of cerebrospinal and fluid serum NSE and MBP are positively correlated with injury severity,which can serve as the basis for diagnosis and prognosis judgment.
7.Micro satellite instability in the development of gastric cancer
Xiaoyong ZHANG ; Ping LIU ; Yun SHAO ;
Chinese Journal of Digestive Endoscopy 2001;0(02):-
Objective To detect the micro satellite instability (MSI) from the precancerous and cancerous lesions (GC) and its effect on carcinogenesis.Methods Silver staining single strand conformation polymorphism polymerize chain reaction (PCR SSCP) was used to screen MSI markers at 5 loci in formalin fixed,paraffin embedded tissues of GC (n=30), dysplasia (n=30), intestinal metaplasia (IM) ( n =40) and corresponding normal gastric tissues.Results The abnormal shifting of the single strand DNA was identified in 7(23 3%)out of GC, in 9 (30%) out of dysplasia and in 8(20%) out of IM samples respectively. GC with MSI was associated with distal location of the tumors ( P =0 044). MSI was not detected in low grade IM tissues.Conclusion MSI may be of an early event during gastric carcinogenesis. It contributes probably to the acquisition of a transformed cell phenotype and to the development of gastric cancer.
8.Catheter ablation guided by three-dimensional electroanatomic mapping system in combination with pulmonary vein antrum potential in patients with atrial fibrillation
Xiaomei LI ; Fuli HU ; Xiaoyong QI ; Meixia LIU ; Bing LIU
Chinese Journal of Tissue Engineering Research 2009;13(52):10313-10317
BACKGROUND:Presently used pulmonary vein antrum location methods mainly performed by three-dimensional electroanatomy combined with X-ray image or CT image fusion.These methods conducted vein antrum location and ablation by anatomy instructions.It is still poorly understood whether the ablation hit the key part of atrial fibrillation.OBJECTIVE:To explore the feasibility of catheter ablation guided by three-dimensional electroanatomic mapping system in conjunct with pulmonary vein antrum potential in patients with atrial fibrillation.DESIGN,TIME AND SETTING:The verification clinical study was performed at the Department of Cardiology of Jinan Fourth People's Hospital and Hebei People's Hospital from March 2007 to June 2009.PARTICIPANTS:Fifty-one patients with drugs refractory,paroxysmal atrial fibrillation were included.METHODS:All patients underwent circumferential pulmonary vein antrum ablation guided by three-dimensional electroanatomic mapping system (CARTO) in conjunct with pulmonary vein antrum potential with the endpoint of electrical isolation.Relevant parameters and ablation success rate were observed.MAIN OUTCOME MEASURES:Procedure-related parameters,such as procedure duration,fluoroscopy duration,cumulative success rate and complication were observed.RESULTS:Pulmonary veins were isolated in all 51 patients.The mean procedure duration,fluoroscopy time and radiofrequency ablation duration are respectively (207±36.7) minutes,(38.2±14.3) minutes,(56.4±15.7) minutes.After (17.5±3.8)-months follow-up,forty (78.4%) patients did not have recurrence of atrial fibrillation,atrial flutter or atrial tachycardia.No severe procedure-related complication had happened.CONCLUSION:Pulmonary vein antrum potential can be used as a landmark to define pulmonary vein antrum,that combined with CARTO system to guide pulmonary vein antrum ablation is effective,safety and feasible for catheter ablation of atrial fibrillation.
9.Frozen heterogeneous periosteum can guide bone tissue regeneration
Xiyun LIU ; Dehui ZHANG ; Hui ZHANG ; Conghao ZHOU ; Xiaoyong LIU
Chinese Journal of Tissue Engineering Research 2007;0(10):-
AIM: To explore the feasibility of bone tissue regeneration guidance with the frozen heterogeneous periosteum. METHODS: The experiment was conducted in the animal laboratory of Beijing Shijitan Hospital from March to December 2002. ①Preparation of the frozen heterogeneous periosteum: The fresh pigs' cranial or rib bone periosteum was taken and washed with saline solution repeatedly, then defatted by immersion in 0.75 volume fraction alcohol, followed by frozen in the liquor of nitrogen for 10 minutes repeatedly for 3 times. After irradiated under ?-ray up to the amount of 3 kGy, the created membrane was placed in refrigerator for using. ②Surgical procedure: Two artificial mandibular bone defects were performed in 30 adult Japanese white big ear rabbits. One defect was covered with the created membrane as the experimental group, while another as the control group. ③Index observation: The rabbits were sacrificed after 2, 4, 8, 12, 16 weeks postoperatively for macroscopic, radiographic and histopathological observation. RESULTS: All 30 rabbits were involved in the result analysis. ①Macroscopic examination of the two groups: At the eighth week, guiding membrane was observed with irregular border. The experiment group was flat and stiff, while the control group was restored but with soft bone; at the twelfth week, the guiding membrane and surrounding border were unclear, but still could be observed. The newly bone of the experiment group was flat and stiff, while the control group was soft with hollow. ②Histopathological examination: At the eighth week, the new-formed bone in the margin of defect area of the experimental group grew intensively and the inflammatory cells in grafted membrane disappeared; intramembrane fiber was found oedema and broken; at the twelfth week, the trabecular bone of the experimental group was thick and arranged regularly. However, in the control group, the newly formed bone was little in the defect, and the trabecular bone was thinner compared with the experimental group. No evidence of inflammatory reactions was apparent, meanwhile the membrane degraded gradually. ③Radiograph examination: The bone density of the experimental group was greater than the control group at 4 and 8 weeks, and had no significant difference as to the vicinity bone at 12 and 16 weeks; on the other hand, the density of the control group was still lower. CONCLUSION: The frozen heterogeneous periosteum is a useful guiding membrane material for tissue regeneration, because it has no obvious rejection action in body, and could maintain its shape for 8-12 weeks, prevent the fiber tissue from growing into the wound surface, separate different cells and guide tissue regeneration.
10.Comparison of acute Mycobacterium tuberculosis infection mouse models established by different infection routes
Fang LIU ; Hua YANG ; Wenjiang ZHOU ; Xiaoyong FAN ; Decheng WANG
Chinese Journal of Infectious Diseases 2012;30(2):76-80
ObjectiveTo compare three types of acute Mycobacterium tuberculosis infection mouse models established through different infection routes and to set up the theoretical basis for further developing,selecting and applying these animal model in the tuberculosis-related research.MethodsStandard strain of Tubercle bacillus H37Rv was diluted to 1 × 106 colony forming unit (cfu)/mL.The mice were infected with the bacteria through different routes including intravenous injection,intranasal administration and inhalation of bacteria aerosol.Six weeks after the infection,the mice were euthaniz ed and necropsied. The lung tissues were collected and gross changes were observed.The colony counting was performed and the lung tissues were assessed by HE staining,acid fast staining.The e xpression level of tumor necrosis factor (TNF)-α per unit area in lung tissue was detected by immunohistochemistry. The data were analyzed by t test. Results The amounts of Mycobacterium tuberculosis in lung tissues of mice in inhalation group,intranasal administration group and intravenous injection group were (6.290±0.028),(6.150±0.021) and (6.120±0.008) lg cfu/mL,respectively; while no Mycobacterium tuberculosis was detected in control group. The difference between infection group and control group was statistically significant (t =3.762,P<0.01),while there were no significant differences among infection groups with different infection routes (P>0.05).According to the results of gross observations and histological assessment,the pathological changes were observed and red tubercle bacillus was detected by acid-fast staining in the lung tissues of all the mice in infection group.The results of immunohistochemistry showed that the expression levels of TNF-α per unit area were as follows:intravenous injection group (0.049 × 106 )<intranasal administration group(0.759×106) < inhalationgroup(1.042×106), whichwere statistically different (t =2.504,P< 0.05).ConclusionInhalation of bacteria aerosol may be the most efficient method to establish tuberculosis infection mouse model compared to intravenous injection and intranasal administration.