Objective:To investigate the etiologic characteristics,as well as the sensitivity of bacteria to antibiotics,in ventila-tor-associated pneumonia(VAP)in Department of Critical Care Medicine,so as to provide evidence for empirical antimicrobial treatment.Methods:The bacterial infection,as well as the sensitivity of bacteria to antibiotics,in 32 VAP cases in Department of Critical Care Medicine in The Third Hospital,Soochow University from Jan 2012 to Dec 2013 were retrospectively analyzed. Results:A total of 110 strains of bacteria were found from 32 VAP cases,including 48 gram negative bacilli(43.6%),43 gram positive cocci(39.1%)and 19 fungi(17.3%).Gram negative bacilli mainly consisted of Baumanii and Klebsiella,Pseudomonas aeruginosa and Escherichia coli.Gram positive cocci mainly consisted of Enterococci and Staphylococcus aureus,Staphylococcus epidermidis and Staphylococcus haemolyticus.Fungi mainly consisted of Candida albicans.Drug sensitive test results showed that Baumanii,Klebsiella and Escherichia coli were more sensitive to carbapenems than to other antibiotics and no Enterococci and Staphylococci was found resistant to vancomycin and linezolid.Conclusions:More VAP patients in Department of Critical Care Medicine were infected by gram negative bacilli.In clinical,empirical antimicrobial treatment should be selected reasonably according to the bacterial etiology and the sensitivity of bacteria to antibiotics,so as to control the infection in Department of Critical Care Medicine and reduce the emergence of drug resistant bacteria.