Breast cancer is the most frequent malignant tumors in women,The p53 gene is found to be one of the most closely associated tumor-suppressor genes in human malignant tumors.Some studies have shown an association between polymorphisms at the forth exon (codon 72) and human malignancies recently.At present, the domestic investigations concerned the p53 polymorphisms mainly in lung cancer, uterocervical cancer,esophagus cancer,ovarian cancers,but the studies in breast cancer are rare in domestic.The correlation between the genotypes of p53 codon 72 polymorphism and breast cancer were reported.

Some published research reports on phthalate esters (PAEs) in the municipal sewage sludge were reviewed in the present paper, and aimed at the problems in the field, some preventive measures and the related research emphases were put forwarded also.

Objective To evaluate our results of external quality assessment (EQA) program of the newborn screening laboratory for 6 years, summarize the problems in the screening measurement and improve the quality of measurement.Methods The phenylalanine (Phe) and thyrotropin (TSH) values in dried blood spot specimens on filter paper distributed by national center were measured by fluorometric method and time -resolved fluorescence im-munoassay ( DELFIA ) respectively.The results sent by national center were analyzed .Results In the 90 specimens of 18 batches, the bias range of Phe and TSH measurement was wide (-62.50%~66.67%and-66.67%~54.31%respectively) and their average values were 8.62%and 10.18% respectively;85 quantitative results of Phe and 84 quantitative results of TSH measurement were in the quality control , accounting for 94.44%and 93.33%respective-ly.The qualitative analysis of 90 Phe and TSH specimens all conformed to the expected results , accounting for 100%.The result of every batch was up to 80%.Conclusion Our neonatal screening laboratory is in control and it is useful to evaluate the measurement competence , find out the problems in the Phe and TSH measurement and re-solve them in time through participated in the national EQA in neonatal screening laboratory , so as to improve the quality of measurement and ensure the accuracy and reliability of results .

As an important part in hospital quality management,medical metrology management is a technical support and necessary means for medical quality.Four aspects such as reinforcement of organization,rules,basic construction and exertion of function are discussed.It is impsrtant and esstntial to strengthen medical metrology.[Chinese Medical Equipment Journal,2008,29(2):107,109]

Objective To investigate chromosomal aneuploidy of chorionic villior tissue from embryo arrestor spontaneous miscarriage by the method of multiplex ligation-dependent probe amplification(MLPA), and to provide genetic etiology data for recurrent spontaneous abortion(RSA) and embryo arrest.Methods The clinical retrospective analysis was used during November 2013 to October 2016 in Shenzhen Maternity and Child Healthcare Hospital.The genomic DNA of 7 036 chorionic villi or tissue samples from embryo arrestor pregnancy loss were extracted by resin and Proteinase K, following detected by multiplex ligation-dependent probe amplification, and then analyzed by descriptive and frequency statistic using SPSS17.0 software.Results Totally 2 984 samples (42.41%) with chromosomal aneuploidy were detected, of which, the top five were trisomy 16(826)11.74%, monosomy X (Turner,401)5.70%, trisomy 22(247)3.51%, trisomy 13(149)2.12% and trisomy 21(144)2.05%.Aneuploidy 19 or 17 were rare,just 1 and 3 cases respectively.The aneuploidy of chromosome 1 was not discovered yet.There were 161 samples (2.28%) for segmental deletion and/or duplication cases.Besides of the monosomy X(Turner,401), monsomy 21 was also high frequency(17,0.24%).In addition, 56 samples(0.80%)were double trisomic,while 2 samples(0.03%)were tris-trisomy.Thirteen samples(0.18%)were heterosome trisomy,and five samples(0.07%)were Turner combined trisomy.Fifteen samples(0.21%) were unbalanced translocation of the arm,while 20 samples(0.28%) were nonhomologous chromosome translocation.Conclusions Trisome and monosome were the majority of chromosome abnormality (above 90%), as well as the main cause of spontaneous miscarriage.Meanwhile the chromosome aneuploidy (including segment deletion or duplication) could be screened by MLPA quickly, thoroughly and efficiently, further more discover the carrier of balanced translocation in couples and provide valuable genetic information for next pregnancy.

Objective To study the the application and influence to quality of life of Kangfuxin liquid combined with psychological intervention in the patients with recurrent oral ulcer. Methods In Wenzhou people's hospital from February 2016 to February 2017, 62 patients suffering from recurrent oral ulcer were divided into two groups. The control group were given Kangfu liquid, at this basis, the observation group were received Kangfuxin liquid combined with psychological nursing intervention. The effect and quality of life in the two groups were compared. Results The cure rate was 93.5％(29/31) in the observation group, while 90.3％(28/31) in the control group. The difference of the cure rate in the two groups was no statistically significant. The scores of physical condition in the observation group were significantly higher than those in the control group (P < 0.05). Conclusion Kangfuxin Liquid combined with psychological nursing intervention can effectively improve the quality of life in the patients with recurrent oral ulcer, which is suitable for clinical application.

Objectives To investigate the regularity of fluctuation and clinical senses of serum c reactive protein (CRP) level in patients with acute cerebral infarction (ACI) Methods 100 subjects (56 males and 44 females) were divided into five groups including 24 large artery arteriosclerosis (LAA), 27 small artery occlusion (SAO), 2 cardiac embolism(CE), 34 other Certain(OC) and 13 undetermined etiology according to Trial of ORG 10172 in Acute Stroke Treatment (TOAST) Through enzyme linked immunoabsorbent assay (ELISA), we detected and analyzed CRP levels of 100 inpatients Results The risk factors of stroke included coronary heart disease , hypertension, diabetes and intoxication of CO and so on Among the above mentioned, the level of CRP and rate of positive CRP of patients who poisoned by CO was the highest That of coronary heart disease group was lower However, there was no notable difference between hypertension and diabetes groups Conclusions CRP may play a certain role in the occurrence and development of ACI It represents different levels in different types of stroke

OBJECTIVE:To establish a method for content determination of bavachin and bakuchiol in Psoralea corylifolia.METHODS:HPLC method was adopted.The determination was performed on Shim-Pack VP-ODS column with mobile phase consisted of acetonitrile-water (gradient elution) at the flow rate of 1.0 mL/min.The detection wavelength was set at 246 nm,the column temperature was 30 ℃,and the sample size was 10 μL.RESULTS:The linear ranges of bavachin and bakuchiol were 48.9-489 ng(r=0.999 8),784-7 840 ng(r=0.999 9).RSDs of precision,stability and reproducibility tests were all lower than 2.0％.Average recoveries ranged 95.30％-99.63％ (RSD=1.45％,n=9),96.89％-100.82％ (RSD=1.36％,n=9).CONCLUSIONS:The method is simple,accurate,stable and reproducible,and can be used for the simultaneous determination of bavachin and bakuchiol in P corylifolia.

Objective To discuss the sedative and hypnotic effect of Jiaotai Pills on insomnia in PCPA rat model .Methods The PCPA ( PCPA acid) rats insomnia model was established, and the rats were divided into the model group, high, middle and low dosage of Jiaotai Pills group, and diazepam positive control group.The high performance liquid detective method was adopted to observe the effect of Jiaotai Pills on rat insomnia and hypothalamus neurotransmitters 5-HT and GABA.Results Compared with the model group, there existed significant difference in the insomnia state of rats and the central neurotransmitters 5 -HT in the high and middle dosages of Jiaotai Pill group and diazepam group.However, in the comparison of the high and middle dosages of Jiaotai Pill group and the model group, there was no significant difference in GABA in the central nervous system .Conclusion Jiaotai Pills has sedative and hypnotic efficacy.Its mechanism may be related to the effects of central neurotransmitter 5 -HT.Its relationship with GABA in the central nervous system is not obvious.The research has significant influence on the further development of Jiaotai Pills.

Objective To study the diagnostic value of TCD and MRI in transient ischemic attack (TIA) of internal carotid artery system.Methods The clinic data was analyzed to contrast the relation of the clinic manifestation and the TCD and MRI results of 66 patients suffered from TIA of the internal carotid artery system.then chi-square test was adopted.Results In TIA interval, extensive hemodynamics changes were occurred in the intracranial arteries among 60 patients(90 9%), and 22 patients(33 3) presented different degrees of stricture on the corresponding symptom side, MRI test showed lacunar infarct or small infarct foci among 50 patients(75 8%), and ischemic leukoenporosis among 35 patients(53 5%). But the foci took a two-side distribution and were relatively symmetrical. No significant correlation existed between the symptom and the foci,and the positive rate of TCD did not show remarkable difference between the symptoms and the foci, and the positive ratio of TCD did not show remarkable difference whether the MRI infarct foci existed or not. Meanwhile, there were also no notable distinction between the stricture change of arteries in the corresponding symptom side with the infarct foci and that of arteries in the corresponding symptom side without the infarct foci.Conclusions TCD can find the change of arteries related in TIA of the internal carotid artery system, and can be used as routine and primary screening. While MRI may bear no special clinic significance to TIA of the internal carotid artery system, but it has localization meaning to CWS, a clinical subtype of TIA.