Objective To develop a HPLC-MS/MS method for the determination of aconitine and study thein vitro metabolic stability of aconitine in dog tissue homogenates.Methods The chromatographic separation was performed on a C18 column.The mobile phase consisted of acetonitrile and water with 0.2％ formic acid and 5 mmol/L ammonium acetate.A triple quadrupole tandem mass spectrometer equipped with an electrospray ionization interface source was used for the quantitative determination in the positive selective reaction monitor mode.Aconitine was incubated with dog tissue homogenates and samples were withdrawn at different time points and precipitated by acetonitrile with internal standards citalopram.Results Aconitine showed good linear relationship over the range from 5 to 500 ng/ml.The recoveries of aconitine were between 85.73％ and 92.12％ at three QC concentration levels.The intra- and inter-day precisions were 5.32％ - 8.95％ and 5.45％ - 8.86％,respectively.After incubation,about 20％ of aconitine were cleared in the liver and small intestine,and t1/2 were 460.6 and 521.3 min,respectively.But none was metabolized in the stomach and kidney.Conclusion These results demonstrated that aconitine was mainly metabolized in the liver and small intestine at a slow rate.

Objective: To pre-assess the occupational hazards in a waste ecological landfill project using comprehensive exposure index with analogy method,and to provide reference for improvement of protective measures and prevention of occupational diseases. Methods: Some enterprises which were comparable with the project in landfill storage capacity,disposal scale,techniques,occupational hazards and occupational health management were selected to carry out on-site occupational health survey,and were evaluated by occupational exposure limits（OEL）. The risk of occupational hazards of the project was assessed by analogy and comprehensive index method. Results: The occupational hazards of analogous enterprises were hydrogen sulfide,ammonia,other dust and noise in ash solidification workshop,paving post in landfill area,spraying post and inspection post in sewage disposal area;manganese and its inorganic compounds,welding fume and arc light in welding post. Except for the noise intensity of spraying post exceeding the national OEL,the other items were qualified. The results of the pre-assessment of the project showed that in the posts with vacant OEL,the hazards of paving compaction post exposed to high temperature in summer,spraying post exposed to high temperature in summer,cypermethrin and machine repairing post exposed to heat,ozone and noise were at moderate risk;in the posts qualified by OEL,the hazards of paving compaction and sewage disposal posts exposed to noise were neglectable,paving compaction and transportation post exposed to other dust were at low risk,others were at moderate risk;unqualified spraying post exposed to noise was at moderate risk. Conclusion Medium-risk occupational hazards exist in paving compaction,spraying,sewage disposal and machine repairing posts. Comprehensive index method combined with analogy method can comprehensively achieve the occupational hazard pre-assessment of a proposed project.