Objective To investigate the effect of systematic training on the enhancement of knowledge and behavior on tumor-free technology among newly-employed nurses in operation room.Methods Fifteen nurses newly-employed for the operating room of the Affiliated Hospital of Luzhou Medical College were trained with theory teaching,techniques training and nursing rounds. The test on non-tumor knowledge and questionnaire on beliefs and behaviors were used to assess the post-training effects.Result The scores on tumor-free knowledge,beliefs and tumor-free behaviors were all significantly improved compared with pre-training (P<0.01). Conclusion Systematic training of tumor-free technology may improve the mastery of tumor-free knowledge and skills and thus form a good tumor-free concept so that they can keep tumor-free techonology in practice.

Objective To assess the influence of changes in alveolar ventilation on gastro-intestinal perfusion measured via a nasogastric tonometer.Methods Sixteen ASA Ⅰ - Ⅱ patients ( 7 male, 9 female) aged 15-67 yr, undergoing elective intracranial operation were included in this study. The patients were premedicated with oral ranitidine 150 mg and intramuscular phenobarbital 0.1 g and atropine 0.5 mg. Anesthesia was induced with midazolam 1-2 mg, fentanyl 2 ?g? kg-1 , propofol 1.5-2.0 mg ? kg-1 and vecuronium 0.1 mg ? kg-1 , and maintained with 0.5%-2.0% isoflurane inhalation and intermittent iv boluses of fentanyl and vecuronium. The patients were intubated and mechanically ventilated after induction of anesthesia. The ventilatory settings were : RR 10 bpm; FGF 1 L? min-1; I: E = 1:2; the initial VT was 7 ml? kg-1 (T1 ) which was gradually increased to 8 ml ?kg-1(T2), 9ml?kg-1(T3) and 10 ml?kg-1 (T4) and then returned to 7 ml ? kg-1 again. Each VT was maintained for 1 h. A nasogastric tonometer (Tonocap, Datex-Ohmeda, Finland) was inserted into stomach and automatically measured gastric intramucosal CO2 tension (PgCO2) every 10 min. Radial artery was cannulated for direct MAP monitoring and blood sampling. Blood gas analysis was performed every hour at various VT levels. Gastric-to-arterial pH gap and gastric-to-arterial PCO2 gap [P(g-a)CO2] were calculated.Results PgCO2 decreased during T2_4 (hyperventilation phase) (P

Objective To observe and analysis the features of images of fundus fluorescein angriography (FFA) in low-perfused retinopathy caused by cephalo-cervical peripheral vascular stenosis or occlusion. Methods The results of FFA of 27 patients diagnosed with carotid artery stenosis or occlusion by digital subtraction angiography (DSA) and examination of Doppler and vascular-pulsation were retrospectively analyzed. Result All of the patients had a delayed arm-retinal circulation duration from 20.0 to 81.08 seconds with the mean of 32.1 seconds; a delayed retinal arteriovenous filling duration from 6 to 64.0 seconds with the mean of 24.2 seconds. Delayed arm-retinal circulation duration and retinal arteriovenous filling duration in 10 cases (37.0%); microangioma, vascular wall staining, nonperfused capillary area in 11 (40.7%); and anterior ischemic syndrome in 6 (22.2%) were found. In the 6 patients with anterior ischemic syndrome, 4 cases had narrow retinal artery, segmental changes of blood stream, vascular atresia, and abnormal arterio-venous anastomosis, and 2 cases had bold vascular loops. Conclusions The main manifestations of FFA in patients with low-perfused retinopathy are malperfusion and retinal ischemia, whose degrees relate to the extend of carotid artery stenosis or atresia, and the process of the disease.Serious retinal ischemia may combined with anterior ischemic syndrome.

Objective The present study was designed to compare the effects of different intra-abdominal pressure and the duration of pneumoperitoneum on splanchnic perfusion during laparoscopic cholecystectomy. Methods Fifty ASA Ⅰ or Ⅱ patients aged

Objective To explore the anti-convulsion action of histamine(HA) in the central system and treatment of histamine H3 receptor(H3R) antagonists to rat model with intractable epilepsy.Methods 88 Wistar rats(12-day-old) were randomly divided into three groups:normal control group,N-methyl-D-aspartate(NMDA) group and betahistine(BH) groups(including high and low dose BH groups).Wistar rats received an intraperitoneal NMDA administration to make animal model of intractable epilepsy at infant period and toddler age.After that,the rats were observed daily for latencies and incidences to two NMDA-dependent stereotypical behaviors.The HA content of each brain region was determined with fluorimetry,and H3R were evaluated with immunohistochemical method.Results The automatisms including tail twisting and emprosthotonus seizures of(12-17)-day-old rats were observed in NMDA and BH groups.The rats,aged 18-25 d,became quiet following automatisms rather than emprothotonic.Compared with NMDA group,BH groups had longer latencies and lower incidences of tail twisting and emprosthotonus(P0.05).Conclusion The NMDA-induced model is similar to the clinical manifest of human West syndrome.It is up to animal model of intractable epilepsy at infant period and toddler age.The HA content of brain region is negatively related with seizure incidence.H3R antagonists have certain therapeutic function to intractable epilepsy in rats at infant period and toddler age.

Objective Controlled hypotension induced by different drugs may have different effects on splanchnic perfusion. The purpose of this study was to assess the effects of nicardipine- induced hypotension on splanchnic perfusion. Methods Twenty-three ASA Ⅰ-Ⅱ patients (14 male, 9 female) aged 21-60 yr undergoing meningioma resection were studied. The patients were premedicated with ranitidine 150 mg per os and atropine 0.5 mg i.m. Anesthesia was induced with propofol 1.5-2.0 mg?kg-1 , fentany1 2.0?g?kg-1 and vecuronium 0.1 mg? kg-1 and maintained with isoflurane inhalation and intermittent i. v. boluses of fentanyl and vecuronium. The patients were mechanically ventilated after tracheal intubation and PETCO2 was maintained at 33-35 mm Hg. Nicardipine-induced hypotension started from opening of mininges to the resection of tumor. 0.01% nicardipine infusion was started at 30 ml?h-1 and adjusted to reduce systolic blood pressure by 30% of the baseline or MAP to≥60 mm Hg. The adequacy of splanchnic perfusion was defined by gastric intramucosal CO2 tension (PgCO2), the intramucosal pH (pHi) and the difference between intramucosal and arterial PCO2(PCO2 gap) and was assessed before induction of anesthesia, the 1st, 2nd and 3rd hour during controlled hypotension and 1, 2 hour after termination of controlled hypotension. Results The MAP was maintained at about 62 mm Hg during controlled hypotension. The heart rate was stable. Hct was significantly decreased at 1st, 2nd and 3rd hour of hypotension compared with baseline but the mean Hct was greater than 30% . The intramucosal pH decreased slightly with no statistical significance and pHa, PaCO2 , PgCO2 and PCO2 gap were almost unchanged during controlled hypotension compared with the baseline values. Conclusion Nicardipine-induced hypotension is safe when MAP is maintained at≥60 mm Hg and has no adverse effects on splanchnic perfusion.

Objective To investigate the effect of clinical nursing paths on function rehabilitation guiding of burn patients. Methods 90 burn patients were randomly divided into observation group (n=45) and control group (n=45). The observation group was guided rehabilitation with clinical nursing paths, while the control group with routine way. Results The scores of Barthel index, and physiological factors,psychological factors, social relations and environmental factors of World Health Organization Quality of Life-BREF improved more in the observation group than in the control group (P<0.001). Conclusion Application of clinical nursing paths to guiding function rehabilitation of burn patients can improve the activities of daily living and quality of life of burn patients.

Objective: To establish the method of fingerprint analysis on Flos Carthami by HPLC. Methods: The HPLC method was used, chromatography conditions were ODS column, CHOH 3 0.4%H 3PO 4 solution (50∶50) as mobile phase and 380nm at detective wavelength. Results: The result showed that 9 peaks (RSD≤3%, n=10 ) were common.Conclusion: This method is reliable, simple and provides a reference standard for the quality control of Flos Carthami.

Objective:Analysis of two ethnic Uygur and Han investigate ischemic stroke adiponectin (Adiponectin) gene single nucleotide rs182052 , rs6444175 , rs1501296 allele polymorphism point whether the differences.Methods:Gene sequencing methods were used to detect 210 was used cases of acute ischemic stroke patients ( case group ) and 104 healthy people ( control group ) Adiponectin gene ,using case-control association analysis of genotype and allele frequencies compared explore the two ethnic differences in different gene polymorphism loci.Results: When the case group and the control group were compared , it was found that the Adiponectin gene rs64441759G/A was significantly higher in the case group.The risk rate increased significantly to 1.481 times (OR=1.481;95%CI:1.219-1.910; P=0.000).Conclusion: Adiponectin gene rs6444175A allele may be susceptible to pathogenic gene.No significant differences show on the 3 SNP loci of Adiponectin genes between Uygur and Han patients.

Objective To investigate the effect of WeChat on nutrition instructions to women of advanced maternal age with gestational diabetes mellitus (GDM). Methods Eighty women of advanced maternal age with GDM in our hospital during January to December 2014 were divided according to their registration nubmer into two group in eqaul number: those with even number in the observation group and those with odd number in the control group. Those in the control group took part in the health educaiton lectures by the parturient school and those in the observation group were instructed on the WeChat besides education from the school. The two groups were compared in terms of blood glucose control and delivery outcomes. Result The level of blood glucose in the observation group was significantly lower than that of the control group (P<0.05) and the rates for cesarean delivery and vaginal birth were significantly lower and the rate of spontaneous labor was significantly higher than those of the control group (P>0.05). Conclusion Wechat is effective in providing the women of advanced maternal age with nutrition instructions and beneficial for the control of blood glucose level and the decrease of cesarean delivery and vaginal birth rates.