Objective:To study the feasibility,safety and effectiveness of intrapericardial injection with a trans-diaphragmatic approach in rats and its value in the study of heart diseases. Methods:Blue-black ink,recombinant adeno-associated virus carrying enhanced green fluorescent protein((eGFP)) gene,0.9% sodium chloride solution were respectively injected into pericardium of rats in three different groups(Group ink,Group eGFP and Group sodium chloride) by intrapericardial injection with a trans-diaphragmatic approach.Autopsy was done and hemodynamic parameters were measured and cryosection was analyzed by fluorescence microscopy. Results:The injection was proved successful in all rats in Group ink.Green fluorescence was detected in cryosection of all hearts in Group eGFP and the expression of green fluorescent protein was ubiquitously,but not homongeneous.No rat died during and after the operation among the operated rats(rats in Group eGFP and Group sodium chloride).There was no difference between hemodynamic parameters of the operated rats and those of the controls.Conclusion:The intrapericardial injection with a trans-diaphragmatic approach is a simple,feasible,safe and valid operation and suitable for small experimental animals.And it suggests an easy, safe,efficient and cheap technique in the study of heart diseases.

2016.0 ng/L group (n=85) and followed up for average 371 days (90-540 d).Cardiac death or decompensated heart failure(HF) readmission were counted as advease events end-point for the purpose of this analysis.Results At follow up,76 patients had the cardiac events (26 patients died from cardiovascular causes and 50 patients being rehospitalization).No differences in admission NT-proBNP between patients with and without cardiovascular events.However,patients with high pre-discharge NT-proBNP(3872.0 vs 1306.0 ng/L,P

Objective To study the short-term therapeutic effects of adeno-associated viral gene transfer of sarcoplasmic reticulum Ca2+-ATPase (SRCA2a) on chronic heart failure (CHF). Methods The rats were divided into four groups: control group, CHF group, CHF+eGFP group and CHF+SRCA2a group. CHF rats were obtained by creating descending aortic constriction. 0.9% sodium chloride solution, recombinant adeno-associated virus carrying enhanced green fluorescent protein (eGFP) gene, and recombinant adeno-associated virus carrying SRCA2a gene, were respectively injected into pericardium of heart failure rats in different groups by intrapericardial injection via trans-diaphragmatic approach. 10 days after gene transfer, hemodynamic parameters, the protein expression of SRCA2a and the SRCA2a activity were measured and analyzed respectively. Results 10 days after gene transfer, the protein expression and activity of SRCA2a were increased by 80.7% and 89.9% respectively, but still lower than those in the control rats. The heart function was also improved along with the increase of SRCA2a expression and activity. The left ventricular systolic pressure and the maximal rate of decline of left-ventricular pressure in CHF+SRCA2a group were similar with those of the controls. The left ventricular end-diastolic pressure and the maximal rate of rise of left-ventricular pressure were much improved, but did not reach the level of the controls. Conclusions SRCA2a protein levels and activity are significantly decreased in CHF rats. Adeno-associated viral gene transfer of SRCA2a can improve the function of the heart in short term.

Breast cancer metastasis is the main cause of tumor death and poor long-term effect in clinical treatment. In the process of metastasis, chemokine may play the main role. In the breast cancer cell, some chemokine and receptor were expressed excessively. It has been shown that through the interaction between chemokine and correspondence receptor, phagocyte and lymphocyte infiltration stimulated, tumor cell invasion into normal tissue with angiogenesis, thus the metastasis was formed. This article reviews the mechanisms of chemokine receptor CXCR4 and chemokine RANTES ,IL-8 MCP-1 in breast cancer metastasis.

OBJECTIVE:To establish the quality standard of Compound niubangzi buccal tablets.METHODS:Lonicerae japonica and Paeonia suffruticosa in the formulation were identified by TLC,and the content of arctiin in the tablets was determined by HPLC.RESULTS:The TLC spots were clear,well-isolated and distinctive.The linear range of arctiin was 0.586～5.274 ?g(r=0.999 9)and its average recovery was 98.94%(RSD=1.68%,n=9).CONCLUSION:The established quality standard can be used for the quality control of Compound niubangzi buccal tablets.

To assess the impact of cytochrome P450 (CYP) 2C19*17 allelic variant on platelet aggregation and bleeding risk in Chinese patients with blood stasis syndrome undergoing percutaneous coronary intervention (PCI) and treated with clopidogrel.

Objective:To explore the impact of cytochrome P450 oxidase (CYP2C19) *2 genotype on major adverse cardiovascular events (MACE) after percutaneous coronary intervention (PCI) and impact of MACE on blood stasis indexes .Methods :From Jan 2010 to Jan 2011 ,a total of 355 patients with blood stasis syndrome undergoing PCI were enrolled .All patients took aspirin and clopidogrel conventionally before PCI ,then maintained for one year at least after PCI .According to MACE or not ,patients were divided into MACE group (n=64) and no MACE group (n=291) .Turbidimetry method was used to measure platelet aggregation rate before and one week after PCI ,and polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP ) was used to test CYP2C19 * 2 gene polymorphism .Results:(1) Compared with no MACE group ,there were significant rise in maximum platelet aggregation rate [MPA ,(36.38 ± 14.57)% vs .(44.87 ± 11.63)% ] and residue platelet aggregation rate [RPA , (25.76 ± 12.69)% vs .(34.45 ± 15.58)% ] , P< 0.01 both ;in percentage of CYP2C19*2 gene mutation (GA+AA) (36.43% vs .75.0% ,χ2 =27.832 ,P<0.01) in MACE group .(2) Multi-factor Logistic regression analysis in-dicated that >65 years ,CYP2C19*2 gene mutation (GA+AA) ,renal dysfunction (creatinine>1.5mg/dl) and re-duced left ventricular ejection fraction (LVEF< 50% ) were independent risk factors for MACE after PCI (OR=2.164~5.947 , P<0.05 or <0.01);(3) Binary linear correlation analysis indicated that CYP 2C19*2 gene muta-tion was positively correlated with RPA after PCI ( r=0.437 , P<0.001) .Conclusion:CYP2C19*2 gene mutation affects antiplatelet activity of clopidogrel ,so it′s helps to recognize MACE risk in patients with coronary heart dis-ease and blood stasis after PCI .

Objective To discuss the sedative and hypnotic effect of Jiaotai Pills on insomnia in PCPA rat model .Methods The PCPA ( PCPA acid) rats insomnia model was established, and the rats were divided into the model group, high, middle and low dosage of Jiaotai Pills group, and diazepam positive control group.The high performance liquid detective method was adopted to observe the effect of Jiaotai Pills on rat insomnia and hypothalamus neurotransmitters 5-HT and GABA.Results Compared with the model group, there existed significant difference in the insomnia state of rats and the central neurotransmitters 5 -HT in the high and middle dosages of Jiaotai Pill group and diazepam group.However, in the comparison of the high and middle dosages of Jiaotai Pill group and the model group, there was no significant difference in GABA in the central nervous system .Conclusion Jiaotai Pills has sedative and hypnotic efficacy.Its mechanism may be related to the effects of central neurotransmitter 5 -HT.Its relationship with GABA in the central nervous system is not obvious.The research has significant influence on the further development of Jiaotai Pills.

Objective To study the antitumor mechanism of DHA compound. Methods RT-PCR was used to investigate the effects of DHA compound on H-Ras and pl6 mRNA transcription in gastric adenocarcinoma. Results Compared with control, the content of H-Ras mRNA was decreased and the content of p16 mRNA was increased in gastric adenocarcinoma significantly after treated with DHA compound. Conclusion The transcription of H-Ras gene was decreased and the transcription of pl6 gene was increased in gastric adenocarcinoma after treated with DHA compound and thus the cancer cell growth was inhibited.

Objective To explore the clinical values of strain rate imaging(SRI) in detection of regional function of left ventricular in patients with hypertension.Methods Twenty-two healthy volunteers and 30 patients with hypertension were examined with SRI.Strain rates of left ventricular segments along axis were analyzed by strain curves,and the peak SR at systole,isovolumic relaxation phase,early diastole and atrial contraction were calculated.(Results Compared) with the healthy subjects,patients with hypertension had appreciably lower SR on all walls in systole((P