Objective To determine the usefulness of MR diffusion-weighted imaging(DWI) in evaluating obstructive jaundice.Methods 25 patients with obstructed dilating bile ducts but without other basic hepatic diseases and 11 normal controls were studied byusing DWI.25 patients were divided into two groups(14 patients with jaundice and 11 patients without jaundice) depending on the serumlevels of total bilirubin.ADCs of liver were measured at workstation,and the mean ADCs were compared among the groups of patients and controlsusing one-way ANOVA.Results When b=500 s/mm~2,the mean ADCs of liver in three groups were significantly different(F=25.29,P0.05) between the ADCs in patients without jaundice and in controls.When b=300 s/mm~2,the mean ADCs of liver in three groups were significantly different(F=12.22,P

Objective To investigate the role of activated cylic AMP(cAMP) signaling in chemical-induced podocyte injury.Methods Eight-weeks-old male BalB/C mice were randomly divided into three groups:control group,Adriamycin (ADR) group and Forskolin+ADR group.ADR nephropathy models were established by tail intravenous injection,and part of them were injected Forskolin,an agonist of adenylate cyclase,intraperitoneally.Phosphorylation of cAMP response element binding protein (CREB) was detected by laser confocal microscopy,morphology of foot processes were determined with transmission electron microscope,and WT-1 expression in glomeruli were detected by immunohistochemistry.Conditionally immortalized podocytes were treated with puromycin aminonucleoside (PAN),Exchange protein directly activated by cAMP (Epac) agonist 8-pCPT-2-O-Me-cAMP (2Me),protein kinase A (PKA) antagonist H89 and its agonist pCPT-cAMP(pCPT).Western blot was used to detect the expression levels of Epac,caspase3 and cleaved caspase3.PKA activity was assayed using cAMP-dependent protein kinase detection system.Cell viability was determined by a cell count kit and podocyte apoptosis was estimated by TUNEL staining.Mitochondrial membrane potential was evaluated by JC-1 staining.Results (1)Compared with ADR group,the urine albumin decreased significantly (P ＜ 0.05) among Forskolin + ADR group and the WT-1 positive cells per glomerulus increased obviously (P ＜ 0.05).(2)PAN decreased podocyte number in a time-dependent manner (P ＜ 0.05),pre-treatment with pCPT obviously inhibited PAN induced podocyte decrease (P ＜0.05),but H89 prevented the effect of pCPT in a dose-dependent manner (P ＜ 0.05).(3)JC-1 staining showed that the percentage of podocyte with green fluorescence for control,PAN and pCPT+PAN group were (12.67±2.15)％,(31.35±4.60)％ and (16.96 ± 2.51)％ respectively (P ＜ 0.05),and pretreatment with H89 inhibited the effect of pCPT (P ＜ 0.05).(4) PAN promoted podocyte apoptosis and cleaved caspase3 expression (P ＜ 0.05),and pretreatment with pCPT significantly prevented PAN-induced podocyte apoptosis and cleaved caspase3 expression (P ＜ 0.05).Conclusions cAMP signaling activation ameliorated podocyte injury in ADR nice and PAN-induced podocyte apoptosis,and cAMP/ PKA pathway may mediate these processes.

Objective:To clone human anti digoxin ScFv from a human semi synthetic phage antibody library.Methods:①Digoxin BSA conjugate(Dig BSA) was preparaed by a modified periodate oxidation method.②A semi synthetic phage antibody library was panned against immobilized Dig BSA.Collected clones were analysed by ELISA,inhibition ELISA and DNA Sequencing.Results:①During the four rounds panning against Dig BSA,the enrichment of the eluted phage particles was observed;②Analysis of the eluted clones identified one clone that could bind Dig as well as other digitalis.③Sequencing analysis showed that the variable genes of the positive clone belonged to VH5 and V?1 subgroup respectively.Conclusion:Human Dig specific ScFv that could bind Dig and other digitalis has been cloned from a semi synthetic phage antibody library which may provide a potential reagent for the diagnosis and therapy of Dig toxication.

Objective:Analysis of two ethnic Uygur and Han investigate ischemic stroke adiponectin (Adiponectin) gene single nucleotide rs182052 , rs6444175 , rs1501296 allele polymorphism point whether the differences.Methods:Gene sequencing methods were used to detect 210 was used cases of acute ischemic stroke patients ( case group ) and 104 healthy people ( control group ) Adiponectin gene ,using case-control association analysis of genotype and allele frequencies compared explore the two ethnic differences in different gene polymorphism loci.Results: When the case group and the control group were compared , it was found that the Adiponectin gene rs64441759G/A was significantly higher in the case group.The risk rate increased significantly to 1.481 times (OR=1.481;95%CI:1.219-1.910; P=0.000).Conclusion: Adiponectin gene rs6444175A allele may be susceptible to pathogenic gene.No significant differences show on the 3 SNP loci of Adiponectin genes between Uygur and Han patients.

Objective To explore the clinical significance of the coagulation and fibrolysis parameters changes for the knowledge of complicated thrombosis after chemotherapy in malignant lymphomas. Methods Morning fasting anti-coagulation blood samples were taken to detect plasma prothrombin time (PT), activated partial thromboplastin time (APTT), fibrinogen (FIB) and thrombin time (TT) with automatic coagulation analyzer in 71 hospitalized malignant lymphomas and 20 normal controls. The plasma D-dimer levels of the two groups were detected with immunoturbidimetry. Results The levels of plasma APTT, Fib and D-dimer in 71 malignant lynphomas were (30.44±1.43) s, (3.28±0.20) g/L, (297.05±56.59) μg/L respectively, which were significantly higher than those in normal controls at the levels of (23.72±0.76) s, (2.57±0.22) g/L, (94.50±26.07) μg/L respectively (P ＜0.05). The coagulation and fibrolysis parameters were of no statistic differences between the normal controls and lymphomas of stage Ⅰ and Ⅱ (P ＞0.05). The APTT and Fib levels in lymphomas of stage Ⅲ and Ⅳ were higher than those in normal controls and lymphomas of stage Ⅰ and Ⅱ (P ＜0.05). There are 7 malignant lymphomas complicated venous thrombosis . Of the 7 cases, the FIB and D-dimer levels were higher than those of stage Ⅰ and Ⅱ, furthermore the D-dimer levels were higher than those of stage Ⅲ and Ⅳ. Conclusion The abnormalities of coagulation and fibrolysis parameters occurred in malignant lymphomas with requirement of periodic monitoring. After chemotherapy, the lymphoma patients had a high incidence of venous thrombosis, which need early prevention for prolongation of the survival.

AIM: Research report that bone marrow stem cells mobilized by recombinan human granulocyte colony-stimulating factor (rhG-CSF) can migrate to lesion spot of infarction, thus decrease brain edema and brain injury after cerebral ischemia. But the report about the effect of drug on brain edema after cerebral hemorrhage is rare. This study investigated the effects of bone mar- row stem cells mobilized by rhG-CSF on reducing formation of brain edema and downregulation of matrix metalloproteinase (MMP)-9 in the peripheral area after intracerebral hemorrhage (ICH) in rats. METHODS:The experiments were performed at the Central Laboratory of Luzhou Medical College from March to November in 2006. ① 144 healthy male SD rats, (300?20)g, were provided by Animal Department of Luzhou Medical College. The experimental procedures of disposing animals were accorded with ethical standards. ②Experimental rats were assigned randomly into a sham operation group, a ICH group and a treatment group, equally. According to the method of Yang, rat models of ICH were made by the method cutting off tail of rat to obtain autoblood in the ICH and treatment groups. Rats in the sham operation group received saline instead of autoblood. Rats in the treatment group were administered with rhG-CSF (60 ?g/kg) by intrap- eritoneal injection after 1 hour. ③The water contents and MMP-9 were measured in each group by immunohistochemical method. RESULTS:Of 144 rats, 16 rats dropped out, among which 7 rats were estimated as 0 grade and 9 rats died, and all were supple- mented. ①The water contents were higher in the ICH group than in the sham operation group (P

BACKGROUND:Administration of recombinant human granulocyte colony-stimulating factor(rhG-CSF) is known to diminish cerebral edema and to enhance the new vascularization by mobilizing endothelial progenitor cells in cerebral infarction, then to promote the neurofunctional recovery.OBJECTIVE:To investigate the effects of rhG-CSF on brain edema and new vessels after intracerebral hemorrhage(ICH) in rats.DESIGN, TIME AND SETTING:The randomized controlled animal study was performed at the Central Laboratory, Luzhou Medical College from March to November 2006.MATERIALS:A total of 144 healthy male Sprague Dawley rats were equally and randomly assigned into a sham operation group, a ICH group and a treatment group.rhG-CSF(Xinpeng, Shenzhen, China) was used in this study.METHODS:Rat models of ICH were made by the method of cutting off the tail of each rat to obtain autoblood in the ICH and treatment groups.Rats in the sham operation group were injected with saline.Rats in the treatment group were administered with rhG-CSF(60 ?g/kg) by intraperitoneal injection after 1 hour.Eight rats from each group were studied at 6, 12, 24, 48, 72 hours and 7 days.Brain water content of rats was measured by dry-wet method.CD34+ vessel expression was detected by SP and DAB coloration, immunohistochemical method.MAIN OUTCOME MEASURES:Dynamic changes in brain water content;immunohistochemical results of CD34+ vessels.RESULTS:The water contents were significantly higher in the ICH group than in the sham operation group(t=4.49, P

BACKGROUND: Recombinant human granulocyte colony-stimulating factor (rhG-CSF) can mobilize endothelial progenitor cells and enhance new vessels at cerebral ischemia region. OBJECTIVE: To investigate the effects of rhG-CSF on the new microvascular expressions in rat perihematoma after intracerebral hemorrhage. DESIGN, TIME AND SETTING: A randomized control animal experiment was performed at the Central Laboratory of Luzhou Medical College from March to November 2006. MATERIALS: A total of 72 healthy male Sprague Dawley rats and rhG-CSF were used for this study. METHODS: Seventy-two rats were equally and randomly assigned into the sham operation group, the hemorrhage group, the treatment group. According to rat brain stereotaxic atlas, models of intracerebral hemorrhage were made by infusing autoblood from rat tails. Rats in the sham operation group were infused with saline instead of autoblood. Rats in the treatment group were administered rhG-CSF (60 ?g/kg) by intraperitoneal injection at 1 hour after operation. Rats in the sham operation and hemorrhage groups were left intact. MAIN OUTCOME MEASURES: The microvascular expressions of CD34+ in perihematoma were detected at 6, 12, 24, 48 and 72 hours, 7 days; Four rats in each time point. Microvascular production was measured by changes in CD34. The more the CD34 antigens, the more the new vessels were. RESULTS: In the hemorrhage group, the microvascular expressions of CD34+ were significantly higher compared to the sham operation group (P 0.05). Significant differences were measured at 6, 12, 24 and 48 hours (P

BACKGROUND:Astrocytes serve as a major component of central nervous system,which reacted actively to various damages.The astrocytes were strongly expressed with enhanced activity after intracerebral hemorrhage.The pathophysiological significance of this change is presently the research hotspot.OBJECTIVE:To investigate the effects of recombinant human granulocyte colony-stimulating factor(rhG-CSF) on the astrocytes expression after intracerebral hemorrhage.DESIGN,TIME AND SETTING:A randomized control experiment was performed at the Central Laboratory of Luzhou Medical College from March to November 2006.MATERIALS:Fifty healthy,male,SD rats were randomly divided into sham operation(n=10),model(n=20) and experimental(n=20) groups.The rhG-CSF was purchased from Xinpeng Bioengineering Co.,Ltd.METHODS:According to rat brain stereotaxic atlas,intracerebral hemorrhage model was prepared by the method of cutting off tail to obtain blood.The blood was replaced with physiological saline in the sham operation group.Totally 60 ?g/kg rhG-CSF was administered by intraperitoneal injection at 1 hour after model preparation.There was no injection in the other two groups.MAIN OUTCOME MEASURES:The expression of glial fibrillary acidic protein(GFAP) was detected with with ABC immunohistochemical method at hours 6,24,48,72 and day 7 after intervention.RESULTS:There was not GFAP-positive cell found in the sham operation group.The GFAP was little expressed at 6 hours,increased at 48 hours,and reached a peak at 72 hours(P

Objective To investigate the differences of miRNA-21 and miRNA-146a expression between colorectal neoplasms tissues and serum of the patients with colorectal neoplasm,and the clinical significance was analyzed.Methods The endoscopic biopsy tissues and serum samples of 100 colorectal cancer (CRC),80 colorectal adenoma (CRA)patients and 65 healthy controls were collected.The expressions of miRNA-21 and miRNA-146a were detected by quantitative real time polymerase chain reaction.The relationship between the expression and clinicopathological features were analyzed.And then,the diagnostic value of expression difference of serum miRNA in colorectal neoplasm were evaluated. The Mann-WhitneyU test and Kruskal-Wallis test were performed for comparisons between groups,and the correlation of miRNA expression between tissues and serum was analyzed by Spearman test.Results
The expressions of miRNA-21 in the tissues of CRC and CRA were 8.573 ±0.898 and 7.746 ±1 .183, respectively,which were significantly higher than that of healthy controls 6.160 ±0.835 (U =120.129 and 33.230,both P <0.01).The expressions of miRNA-21 in the serum of CRC and CRA were 1 .829± 0.303 and 1 .624 ±0.226,respeotively,which were higher than that of healthy controls 1 .391 ±0.221 (U =40.353 and 15 .512,both P < 0.01 ).The expression of miRNA-21 in the serum and tissues of patients with CRC were both higher than those of patients with CRA (U =11 .384 and 10.189,both P <0.01).The expression of miRNA-21 in patients with CRC was associated with TNM stage and lymph node metastasis.The expression level of miRNA-21 in CRA was correlated with histological types.The results of Spearman analysis indicated that the expression of miRNA-21 in the tissues and serum of patients with CRC was positively correlated (r=0.459,P <0.01).The expression of miRNA-146a in the tissues and serum of CRC patients were 2.556±0.351 and 0.249±0.038,respectively,which were lower than those of healthy controls (3.428 ±0.328 and 0.279 ±0.053)(U =102.134 and 30.111 ,both P <0.01).The expression in the tissues and serum of CRA patients were 3.255 ±0.332 and 0.290±0.036, respectively,which were also lower than those of healthy controls,however the difference was not statistically significant (U = 3.936 and 3.180,both P > 0.05 ).The expression of miRNA-146a in the tissues and serum of CRC patients were both lower than those of CRA patients (U =73.809 and 21 .123, both P <0.01).The degree of decreased expression of miRNA-146a in CRC patients was correlated with TNM staging and tumor differentiation degree,however there was no correlation between the expression in CRA and clinical features.According to receiver operating characteristic (ROC)curve analysis,the AUC of miRNA-21 ,miRNA-146a and a combination of them in CRC and health individuals was 0.889, 0.791 and 0.863,respectively;in CRA and health individuals was 0.784,0.692 and 0.761 ,respectively;in CRC and CRA was 0.705 ,0.820 and 0.713,respectively.Conclusion The different expressions of miRNA-21 and miRNA-146a had potential values in early detection of colorectal cancer.