Objective To investigate the rehabilitation of disability in Guangzhou.Methods In 2004, Guangzhou government had investigated 85397 disabled persons who have the disable card in 13 district of Guangzhou City. The data about the rehabilitation from this investigation were analyzed, including the rehabilitation accepted, effects, place, operation, etc. Results and Conclusion 66614 cases were investigated about rehabilitative accepted. 54650 cases were investigated about rehabilitative effect. 46808 cases were investigated about rehabilitative place. 44794 cases were investigated about rehabilitative operation. The number of poor effect of rehabilitation was large, because a lot of disabled persons had not been treated with rehabilitation. A lot of rehabilitation were played in family, with few equipment and by no-professional.

Objective To investigate the rehabilitation staffing in community health care center in Guangzhou City. MethodsThe basic personal information, work condition, work load, problems in special staff of rehabilitation for disabled persons were investigated with self-report questionnaires. Results143 staff (92.86%) had accepted higher education, 38 (24.68%) practiced beyond 3 years. The main work was the rehabilitation services for local inhabitants, and the other services have not been carried out widely. The main problems reported were lack of finance, staff and skills training. 83.77% promised that they would provide the rehabilitation services meeting the governmental standard. ConclusionStaff who work for the community health care center have more professional education and experience, compared with years ago. The services need more normalization, and the financial support is also needed.

Objective To reseach the effects of diazepam under different cerebral temperature on the concentration of glutamate(Glu), ?-aminobutyric acid (GABA), superoxide dismutase (SOD), glutathione peroxidase(GSH-PX) and malonaldehyde(MDA) in cerebral ischemia tissue of rat. Methods The modle of cerebral middle artery occlusion reperfusion of rats was established to induce the target cerebral temperatue.The concentration of Glu, GABA, SOD, GSH-PX and MDA were detected in mild hyperthermia, ordinary temperature and subhypothermia groups respectively. Results (1)Compared with the sham-operation group under normal temperature,the concentration of Glu, MDA had increased obviously in the control group of focal cerebral ischemia and the group using diazepam under normal temperature (all P0.05),but the concentration of GABA had increased markedly in the groups of normal temperature using diazepam (P0.05).(3)Compared with the control group using diazepam under normal temperature,the concentration of Glu and MDA increased obviuosly(all P0.05).The concentration of Glu and MDA in the groups of hypothermia using diazepam was lower markedly (P

In recent years,health economics developed quickly,which attracted both international academic and public interested.Compared with the developed countries,although the study of health economics in China started relatively slow,it has already attracted more and more attention by the academic along with the rapidly social development.With the support from international organizations and academic institutions,China had gradually established its health economics research net from 1990s,the research system had been improved and a serious of research achievements had already playedmore important role in the procedure of government decision making.It summarized the research progress in the field of international and China's health economics so as to provide references for research and policies.

BACKGROUND: In the researches of Ginkgo Biloba Extract(GBE) in the treatment of cerebra ischemia, because of the application of generally anaesthesia medication that may induce the alteration of cerebral temperature, the accuracy of the results may be affected.OBJECTIVE: To investigate the impact of domestic GBE on antioxidase and lipid peroxide of cerebral ischemic reperfusion tissue as well as the water content of ischemic brain tissue under normothermia.DESIGN: A randomised controlled trial.SETTING and MATERIALS: Study was conducted in the Tongji Medical University of Huazhong Science and Technology University. A total of 24 Wistar rats with a mass from 250 g to 300 g were randomly allocated into sham-operation group ( n = 8 ), cerebral ischemia control group ( n = 8) and cerebral ischemia GBE treatment group( n = 8) . The animal model of normothermia rat with left middle cerebral artery ischemia for 2 hours and reperfusion for 2 hours was prepared with the reference of Nagasawa method in the animals of control group and treatment group for contrast study.INTERVENTIONS: The cerebral temperature of the rats was reflected by the temperature of the temporal muscle. The temperature-measuring probe was embedded into the deep part of the left temporal muscle closed to osseous ectoblast. The temperature was continuously monitored by semiconductor oxide temperature sensor. The temperature of the head was heated with 60 W filament lamp and adjusted by automatic double-controlling craniocerebral cooling instrument to maintain the cerebral temperature at normothermia level of 36.5 ℃ - 37 ℃. The normothermia cerebral ischemic reperfusion injury rat model was established according to the design. GBE injection was injected respectively into abdominal cavity in the rats of cerebral ischemia GBE treatment group at the following time point: 12 hours, 8 hours and 4 hours before operation, immediately after cerebral ischemia and immediately after reperfusion, with 3 mL each time and 5 times in total. Same times and dose of normal saline was injected into the abdominal cavity in the rats of both control group and sham-operation groups.MAIN OUTCOME MEASURES: Superoxide dismutase (SOD), glutathione peroxidase(GSH-Px), reduced glutathione(GSH), malondialdehyde(MDA)and water contents in the cerebral ischemic tissue.RESULTS: The levels of SOD, GSH-Px and GSH in cerebral ischemia control group were(73.35 ± 12. 86) NU/mg, (167.37 ±54.34) μkat/g and (196. 84 ± 22.75) μg/g respectively, which significantly lower than that (96. 02± 16. 83) NU/mg, (338.57±84.02) μkat/g and(337.51± 34. 89) μg/g of sham-operation group( P ＜ 0. 01 or P ＜ 0.05) . The SOD, GSH-Px and GSH levels of cerebral isehemia GBE treatment group were (87.24± 15.03) NU/mg, (316. 56 ±93.52) μkat/g and(263.16±28.54) μg/g, which significantly higher than that of cerebral ischemia control group(P ＜ 0.01 or P ＜ 0.05) .The MDA level of cerebra ischemia control group was (308.34 ± 26.81 ) nmol/g, which significantly higher than that(101.46 ± 10.97) nmol/g of sham-operation group( P ＜ 0.01 ) .The MDA level of cerebral ischemia GBE treatment group was(125.86± 13.90) nmol/g, which was significantly lower than that of sham-operation group ( P ＜ 0.01 ) . The water content of cerebral ischemia control group was(80. 45 ± 0.44)%, which was significantly higher than that (78.20 ± 0. 25 ) % of sham-operation group ( P ＜ 0.01 ) . The water content of cerebral ischemia GBE treatment group was(79.63 ± 0.46) %, which was significantly lower than that of cerebral ischemia control group( P ＜ 0.05).CONCLUSION: Domestic GBE can inhibit the excessive production of free radicals and the lipid peroxidation during cerebral ischemia and reduce cerebral oedema and the destruction of blood-brain barrier to protect cerebral ischemic tissues under cerebral normothermia.

Objective To evaluate the application of DNA image cytometry in screening cervical intraepithe-lial neoplasia and cervical cancer.Methods 80 patients with cervical intraepithelial neoplasia admitted during No-vember 2012 and November 2014 were involved in this study.The samples from all the patients were taken by cervix brushes and prepared for 2 slides:one for liquid-based cytology staining, read by cytologist, and the other for Thi-onin-Feulgen staining, tested by DNA image cytometry.The biopsy was performed with direction of colposcopy if one test was positive to verify the sensitivity and specificity of the two methods of staining.Results 70 cases were positive by DNA image cytometry and 27 ones were positive by liquid-based cytology.There was statistically signifi-cant difference in the sensitivity between the DNA image cytometry and the liquid -based cytology ( 94.8% vs. 38.8%;p<0.05), but no difference in the specificity between them (99.6%vs.99.8%).Conclusion Besides its good specificity, DNA image cytometry is more sensitive in screening cervical intraepithelial neoplasia and cervical cancer.

Objective To explore the relationship between the pathological classification and clinical manifestations of lupus nephritis(LN)in children by renal biopsy and laboratory examination. Methods Renal biopsy and routine laboratory tests were performed in 35 cases of LN . The pathological classification of LN was made according to the criteria of WHO1982. Results Type Ⅳ of LN was most common(37.4%), and type Ⅴ(31.7%) and type Ⅲ(20.0%) were next. Type Ⅳ and type Ⅴ usually appeared as nephritic syndrome, while Type Ⅱ and Ⅲ mainly appeared as nephritis syndrome. The frequency of hypertension and renal dysfuction was the highest in type Ⅳ. Generally the renal interstitial injury of LN was mild, but that of type IV of LN was relatively obvious. There was a positive correlation between serum creatinine level and the degree of renal interstitial injury. Conclusion Lupus nephritis in childhood possessed some features in renal pathological change and clinical manifestations. Renal biopsy was important to diagnosis, treatment and prognosis evaluation of lupus nephritis.

[Objective] To evaluate the clinical efficiency of refratory edema based on daytime prelonged Online-HDF.[Methods] Six patients include four females and two males whose age ranging from 28 to 74 years old and average 55 years old.Online-HDF treatment for 3-9 times,vascular access was percutaneous femoral or internal jugular catheterization.One patient's temporary access was changed to permanent access because who needed continous hemodialysis.The machine version was Ganbro AK-100S.To filter by u2000 and replace fluid online for 6-10 hous,blood flow was 200-250ml/min,uf 1200-8600,the total fluid used for replacement was 20-42 liter,during the process,regular heparin or low molecular was used for anticoagulation.[Results]Three patients' clinical symptoms resulted from edema resolved,one patient accepted continous hemodialysis treatment for inpaired kidney function,one patient left hospital for expensive medical cost,one patient accepted ventilation treatment for respiratory failure in ICU.[Conclusion]Daytime prelonged Online-HDF is effective on the trentment of refratory edema,especially effective on dyspnea,tachypnea,chest distress that resulted from edema.

OBJECTIVE:To establish the Microbial limit test(MLT) for Danggui funing drop pills.METHODS:The recovery rates of 5 tested strains treated by Danggui funing drop pills were detected and the MLT method for the control bacteria was validated.RESULTS: Danggui funing drop pills exhibited strong inhibitory effect on staphylococcus aureus and bacillus subtilis,but showed no effect on escherichia coli,candida albicans and aspergillus niger.CONCLUSION: By membrane-filter procedure,the antibacterial effect of Danggui funing drop pills can be eliminated and the bacterial count can be conducted;however,by routine method,the test of control bacteria is feasible.