1 000 nmol/L.We should search the cause of disease and perform tumor execution as soon as possible.The short-term prognosis is good.

Objective To investigate the way of gene therapy on congestive heart failure and other heart diseases. Methods Recombinant adeno associated viruses (AAV) encoding the human ? 2 adrenergic receptor(AR) and enhanced green fluorescent protein (EGFP) were constructed and the ? 2 AR and EGFP cDNA were transfected in vitro to the neonate rat ventricular myocytes. The expression of ? 2 AR and EGFP on cultured neonate rat ventricular myocytes and the ability of ? 2 AR to potentiate the beta adrenergic signaling were assessed. Results The presence of human ? 2 AR mRNA was demonstrated by Northern blot, the expression of the ? 2 AR gene was demonstrated by Westernimmuno blot. The density of ? AR in the myocytes was greater than that in the control 〔(204.0 ? 6.4) fmol/mg protein vs (76.0 ? 2.8) fmol/mg protein〕 according to a ligand binding assay. Human ? 2 AR gene significantly increased isoproterenol stimulated cAMP as compared with the control〔(116.2?5.8) pmol/10 6 cell vs (58.4 ? 4.6) pmol/10 6 cell〕. EGFP was also expressed in the transfected cardiomyocytes. Conclusions Human ? 2 AR gene could be transfected into cardiomyocytes by AAV vector and could potentiate the beta adrenergic signaling.

Objective To study the effect of hospital disposal manners after workplace violence in hos-pital on organizational commitment and turnover intention among the head nurses. Methods Self-made questionnaire about workplace violence in hospital and general condition, organizational commitment question-naire and turnover intention questionnaire were conducted in 122 head nurses who had suffered hospital vio-lence in recent years from all level hospitals of Fujian province. Results The scores of affective commitment and normative commitment of organizational commitment and turnover intention showed significant difference be-tween the groups who suffered positive disposal and who suffered negative interference by the hospital after the violence events (P<0.05), but aggregate score of organizational commitment and the scores of continuance commitment showed no significant difference between the two groups (P>0.05). Hospital disposal manners af-ter the hospital violence and hospital grade were influence factors on turnover intention among the head nurses.Hospital grade was also an influence factor on organizational commitment among the head nurses. Conclu-sions Hospital positive disposal means after the hospital violence on head nurse help to ameliorate organiza-tional commitment and reduce turnover intention among the head nurses.

OBJECTIVE:To establish the quality standard of Compound niubangzi buccal tablets.METHODS:Lonicerae japonica and Paeonia suffruticosa in the formulation were identified by TLC,and the content of arctiin in the tablets was determined by HPLC.RESULTS:The TLC spots were clear,well-isolated and distinctive.The linear range of arctiin was 0.586～5.274 ?g(r=0.999 9)and its average recovery was 98.94%(RSD=1.68%,n=9).CONCLUSION:The established quality standard can be used for the quality control of Compound niubangzi buccal tablets.

Objective To study MR findings in 20 children aged from 10 months to 14 years with mitochondrial encephalomyopathy.Methods MRI evaluations were done in 20 children with mitochondrial encephalomyopathy proved by muscle biopsy and biochemical laboratory examinations.Results Brain parenchymal lesions in all cases were low signal intensity on T 1-weighted and high signal intensity on T 2-weighted images.Brain atrophy was showed in different degrees in 8 children.18 patients had involvement of gray matter,10 had only the deep gray matter involved and 4 cases had both the deep gray matter and the cerebral cortex involved simultaneously.4 patients showed deep gray matter abnormalities and cerebral infarction with involvement of cortex and subcortical white matter.2 patients had exclusively involvement of white matter,which were nonspecific white matter changes of the trigonal area.Conclusion MRI findings in mitochondrial encephalomyopathy are varied when gray matter especially deep gray matter involved,brain atrophy,untypical infarction and involvement of peripheral white matter are showed on MR,and associated with a variety of neuromuscular symptoms in children mitochondrial encephalomyopathy should be consider.

Objective We retrospectively studied the MRI and MRA appearances of 10 patients,(6 children,4 adults) with Moyamoya disease.Methods MRI and MRA images were analyzed.For the MRA images,it was concerned that whether there were occlusive changes of the internal carotid artery and middle cerebral artery and whether there were collateral vessels.For the MRI images,the locations of the infarct lesions were studied.Results For the MRA images,the internal carotid arteries and the middle cerebral arteries were observed stenosis,and collateral vessels were formed in cerebral hemispheres.There were no remarkable differences between the children and adult groups with regard to the occlusive changes or to the flow void sign on MRA.However,Moyamoya disease in children exhibited a significant increase in the cortical and subcortical infarction(75%,9/12).In contrast,there were much more deep white matter infarctions in the centrum semiovale and basal ganglia in the adult group(87.5%,7/8)than in the children group(33.3%,4/12).Conclusion MRI and MRA are the method of choice in diagnosis of Moyamoya disease.The differences in the site of infarctions between the childhood and the adult Moyamoya disease observed on MRI may explain the differences in the clinical presentation.

Objective:To research the effect of the media on women's body image.Methods:The subjects were all from 2 colleges.The subjects of experiment 1 were 63 high and low trait social comparison female students.Among them,high trait social comparison subjects were 31 in the model image group,30 in the product image group.Low trait social comparison subjects were 30 in the model image group,32 were in the product image group.The subjects of experiment 2 were the total of 61 high trait social comparison female students.Among them,30 subjects were analyzed by similarity comparison,and 31 subjects received difference comparison.The instruments included advertisement pictures,body image states scale,the physical apperance comparion scale,and the consumer response questionnaire.Results:Analysis of variance showed that,in the model image level,the score of body image scale in high social comparison group was significantly higher than that in low social comparison group [(5.54±1.05)vs.(4.73±0.92),P<0.001].Regression analysis further showed that social comparison was moderator(ΔR~2= 0.035,P<0.01).For social comparisons mode,score of body image scale in difference comparison group was higher than that in similarity comparison group [(5.55±0.82)vs.(4.56±0.86),P<0.05].Conclusion:These findings suggested that social comparison can be used to prevent female college students' negative body images.

Objective:To investigate the brain activation patten associated with visual sexual stimuli.Method:Thirteen healthy right-handed women were enrolled through advertisements on campus.They were scanned under functional magnetic resonance imaging(fMRI) when they watched emotional neutral films excepts and erotic films excepts.Statistic on theses data reveals the difference of brain activation.Result:The results showed that,compared to emotional neutral films,sexual visual stimuli were associated with the activation of a set of bilateral brain areas including lateral occipital cortex,postcentral gyrus,superior parietal lobule,occipital fusiform gyrus, hippocampus,and so on.Most activated brain areas are bilateral.Only in inferior frontal gyrus pars opercularis(Z=3.64,P<0.0001) and pars triangular(Z=3.42,P=0.003) there were significant right hemispheric activation.Conclusion:fMRI results indicate that inferior frontal gyrus is related to the visual sexual stimuli of women subjects,and is specified on the right hemisphere rather than left one.This sphere-special area may play an important role on sexual message processing.

Objective In this retrospective study, we analyzed the magnetic resonance imaging (MRI)and computed tomography(CT)scans of patients with prostate cancer to investigate the relationship between the apex of prostate and the anatomic structures visible in CT, and to provide evidence for localizing the prostatic apex in radiation treatment planning. Methods MRI and CT scans from 108 patients with prostate cancer were analyzed to measure the distance between the prostatic apex and the bottom of ischial tuberosities,the bottom of obturator foramen, the bottom of pubic symphysis and the bulb of the penis. The volume of prostate was calculated and the relationship between the size of the prostate and the localization of the prostatic apex was analyzed. Results The prostatic apex is located 13. 1 mm ±3. 3 mm superior to the bulb of the penis, 11.0 mm ± 5.4 mm superior to the bottom of obturator foramen, 31.3 mm ± 5.5 mm superior to the bottom of ischial tuberosities, and 7. 1 mm ± 4. 7 mm superior to the bottom of obturator foramen. There was no correlation between the size of prostate and the localization of the prostatic apex(R =0. 07、-0. 33, all P ＞ 0. 05). Conclusions Ninety-five percent of patients had a prostatic apex that is above the bulb of the penis 6 mm, and 100% of patients had a prostatic apex that is above the bottom of obturator foramen.

Background　Culture of retinal pigment epithelium(RPE) cells is very important for establishment of proliferative vitreoretinopathy (PVR) model,prevention and treatment of PVR as well as RPE cell transplantation.Isolation of animal RPE cells by trypsinization is a critical step.ObjectiveThe present study is to establish the methods of isolation and culture of retinal pigment epithelium (RPE) cells in rabbit and comparied with that of pig RPE culture.MethodsRPE cells were isolated by trypsinization in pigmented rabbit and pig and cultured in DMEM containing 20% fetal bovine serum.Cultured RPE cells were identified by immunochemistry.The fourth generations of cells were used in this experiment.Morphology and characteristics of cultured RPE cells from rabbit and pig were examined and compared under the light microscope.ResultsIsolated RPE cells from pig were obtained by once trypsin digestion,but two times of trypsinization were needed in rabbit RPE cells isolation.The differentiation in response to trypsinization was related to anatomic difference between the two types of cells .The adherence time of pig RPE cells was 24 hours ,however,the rabbit RPE needed 48-72 hours after culture.Proliferation and vitality of cultured cells were gradually attenuated and melanin decreased after several times subculture.The morphology of culture RPE cells was obviously different between rabbit and pig because species difference.Immunohistochemistry demonstrated the positive response of RPE cells for keratin.ConclusionRPE cells can be acquired from both rabbit and pig by trypsinization and culture.The culture process of RPE cells of pig is simpler than that of rabbit.Cells within the fourth generations are suitable for experimental application.