Currently,the medical technology and service capability in grass-root medical institutions are still at low level.This article emphasizes the need for pilot bases of appropriate medical emergency technologies,introducing our experiences in this respect and the effects.We hope to offer reference for the promotion of appropriate medical emergency technologies.

[Summary] Epigenetics defines heritable changes in gene expression that are not coded in the DNA sequence itself.Three systems,including DNA methylation,RNA-associated silencing and histone modification,are used to initiate and sustain epigenetic silencing.Type 2 diabetes mellitus(T2DM) is a polygenic disorder,which is caused by both genetic and environmental factors.However,pathogenesis of T2DM has not been elucidated.Recent studies have indicated that epigenetics may play an important role in the pathogenesis of T2DM,which is involved in the development and differentiation of β cell,secretion of β cell,insulin sensitivity and so on.Researches related to epigenetics may provide a new measure and target to clarify the pathogenesis of T2DM as well as prevent and treat the disease.

90% in 20 min and more higher than the control tablets or capsule. CONCLUSION: The optimum formulation suits to slightly soluble drugs with different o/w distribution coefficient.It can provide reference for application of the SMEDDS the practical cases.

Objective To observe the effect of breviscapine on the open probability(Po)and the average open time(To) of L-type calcium channel in the hippocampal neurons of the whole brain ischemia-reperfusion rat model.Methods The whole brain ischemia rat model was established.At the reperfusion periods of 1.5 h,3.0 h,4.5 h and 6.0 h,the hippoeampal CA1 neurons were dissociated abruptly and the Ca~(2+)electricity of single channel on the neuron membrane were recorded with cell-attach model of patch clamp techniques.The Po and To of the L-type calcium channel were an- alyzed and the effect of the breviscapine was observed.Results Different concentrations of breviscapine can decrease the Po and To of the L-type calcium channel in hippoeampal neurons,particularly within 3 hours after ischemia-reperfu- sion.Conclusions Breviscapine has protective effect on the cerebral ischemia-reperfusion injury.

ObjectiveTo compare the yield rate of rats islets between different collagenase digestion groups.MethodsThe SD rats were randomly divided into two groups as following by using random digits table:collagenase P group (pancreas digested by 1 mg/ml collagenase P) and type Ⅴ collagenase group (pancreas digested by 1 mg/ml type Ⅴ collagenase).After pancreas digestion,rat islet cells in two groups were culture,purified and stained with DTZ.The mean islet number and islet equivalent (IEQ) before and after purification were measured under an inverted microscope.The viability of purified islets was assessed by fluorescence staining of aridine orange (AO) and propidium iodide (PI) under the fluorescence microscopy.After purification and culture for two days,islets function was evaluated by insulin releasing tests in the two groups.ResultsBefore purification,there was no significant difference in the islets number obtained from the pancreas between two groups (P＞0.05),but there was significant difference in the IEQ (P＜0.05).After purification,the islets number in type Ⅴcollagenase group and collagenase P group was (485 ± 113)/pancrease and (643 ± 82)/pancrease,and IEQ was (674 ± 157)/pancreas and (989 ± 126)/pancreas,respectively (P＜0.05).Islet viability in type Ⅴcollagenase group and collagenase P group was (96.13 ±1.13) ％ and (96.38 ± 0.92) ％ respectively (P＞0.05).The results of insulin releasing tests revealed there was no significant difference in islet function stimulated by hypoglycemia and hyperglycemia between two groups (P＞0.05).ConclusionTwo types of collagenase are suitable for the islets digestion in rats.The stability of digestion and yield rate of purified islets in collagenase P group are higher than in type Ⅴ collagenase group.

Objective To investigate the correlation of dynamic contrast-enhanced (DCE) MRI features with microvessel density (MVD) and vascular endothelial growth factor (VEGF) in non-small cell lung cancer(NSCLC).Methods Conventional MR imaging and dynamic contrast-enhanced scan in thirty-three patients with NSCLC confirmed by pathologyn were performed. MVD and VEGF were stained with immuno-histochemical technique in all cases. Some parameters of DCE MRI, including maximum slope(Smax) and time to peak(TTP) were put more analysis. The relationship between the results of DCE MRI (Smax and TTP) and that of immuno-histochemistry (MVD and VEGF) was analysed.Results The Smax of adeno carcinoma was higher than that of squamous cell carcinoma,but TTP was lower. The difference was obvious difference(t=3.22,P

40 years, old group) without prostate diseases, underwent MRI and MRS examinations. The peripheral zone (PZ) and central gland (CG) of the prostate were distinctly demonstrated on MRI, the right and left side of PZ and CG were divided into bottom, middle and tip regions,totally 12 samples were obtained from each case. The levels of (Cho+Cre)/Cit ratios were calculated on the basis of the MRS metabolic map. Results ①The mean level of (Cho+Cre)/Cit ratios in PZs and CGs of different regions in young group and old group was not significantly different. Statistically significant variation of the (Cho+Cre)/Cit ratio in PZs and CGs in the two groups was detected. ②The mean level of (Cho+Cre)/Cit ratios of PZs between young group and old group was not significantly different. Only Statistically significant variation of (Cho+Cre)/Cit ratios in CGs between two groups was detected. Conclusion The normal levels of prostatic metabolites fluctuate dramatically among individuals. There is metabolic difference between the peripheral and central zone and that of young and old group.

Objective To investigate the MRS characteristic of low T_2 signal in peripheral zone of prostatitis and prostatic carcinoma(Pca) and to evaluate the role of MRS in the discrimination of prostatitis and Pca.Methods After biopsy,24 patients with Hypointense T_2 signal in prostatic peripheral zone were divided into two groups:prostatitis and Pca.3D MRS was performed on the low T_2 area of peripheral zone to measure the ratio of(Cho+Cre)/Cit.Results The mean ratio of(Cho+Cre)/Cit in low T_2 area of prostatitis was 0.98?0.17,whereas that of Pca was 1.41?0.31,statistically significant difference was detected(t=8.89,?

SUMMARY Left ventricular noncompaction(LVNC)is a rare congenital disorder of endomyocardial morphogenesis.Since the knowledge of aetiology and pathology is accumulating,the 2006 AHA cardiomyopathy classification sorts LVNC as one of the primary genetic cardiomyopathies.The clinical features of LVNC,however,is not as clear as its aetiology.We summarized the manifestation,hemodynamics,natural course,diagnosis,therapy and prognosis of LVNC by analyzing its clinical features of 2 cases and reviewing the latest related articles.

Objective To quantitatively evaluate the metabolic changes of prostate adenocarcinoma using magnetic resonance spectroscopy (MRS).Methods Eighteen patients of prostate cancer proved by ultrasound guided systemic biopsy were enrolled in this study.All the puncture locations were marked and the corresponding (Choline+Creatine)/Citrate ratios were calculated on the basis of the MRS metabolic map.Results 204 samples were obtained and 106 of them were diagnosed as cancer.The average (Cho+Cre)/Cit ratio in the 106 positions was 2.53?1.02.The (Cho+Cre)/Cit ratio of cancer area was statistically higher than that of noncancerous area (F=8.64, P