In order to study the disease occurrence , developments and changes in a hospital and the sur -rounding area, statistical analysis of outpatient and inpatient diseases and medical expense from 2008 ~2013 in the hospital was made.According to the analysis results, the principal diseases of outpatient are infectious disease and chronic disease.Dry -eye incidence has been increasing rapidly in the past 6 years, and the medical expenses in-creased significantly, and attention should be paid on it.Among the inpatient diseases , pregnancy, childbirth and puerperium complications consistently rank the first .This is the reason why the highest proportion is around the age of 20 ~39 years old and among the woman sufferers, but in the inpatient disease spectrum , the constituent ratio appears a decrease trend year by year, meanwhile, genitourinary system disease, respiratory system disease, circulation sys-tem disease and tumor show the upward trend year by year .Therefore, the proportion of self -pay medical expenses of patients is still high, and the financial burden is heavy .Hence the construction of medical security system needs to be continually strengthened.

Objective:To investigate whether TRAIL can synergize 5-Fu and DDP to kill HEP-2 cell line.Methods:MTT was used to detecet the cytotoxicity of HEP-2 cell line intervented in 5-FU,DDP,TRAIL,5-FU+TRAIL and DDP+TRAIL.Fluorescence microscope and FCM were used to detecet the apoptosis rate of cell.Results:HEP-2 cell line was insensitive in TRAIL,but the apoptosis rate obviously was upgraded after intervented in 5-FU+TRAIL and DDP+TRAIL.Conclusion:TRAIL in combination with subtoxic dose of 5-FU and DDP can effectively kill HEP-2 cell line.The combination of TRAIL,5-FU and DDP induced apoptosis of HEP-2 cell line.

Objective:To observe the effects of different concentrations of TRAIL and chemotherapeutic drugs(5-FU,DDP) on proliferation and apoptosis of HNE-1 cell lines ,both singlely and jointly.Methods:MTT was used to detect the inhibition rate.FCM was used to detect the apoptosis rate of cell.Results:The inhibition rate and apoptosis rate of combination use of TRAIL and 5-FU,DDP were significantly increased,compared with those of single use of TRAIL.Conclusion:The inhibition of proliferation and apoptosis of HNE-1 cell line can be induced by TRAIL,and the effects were higher by TRAIL combined with 5-FU and DDP.

Objective To make clear the resources of pteridophyte in Zhejiang-Dongqian lake area.Methods Field investigation, data reference and consultation to local peasants were adopted. Results There are 20 famines, 23 genera, and 26 species of medicinal ferns in Dongqian Lake area. Conclusion Medicinal ferns are abundant in Zhejiang-Dongqian lake area.

Objective To analyzed the use of oral hypoglycemic drugs in our hospital for reference of clinical rational use of this kind of drugs. Methods The sorting methods of defined daily dose (DDDs) and the frequency of oral hypoglycenic drugs were used for analysis. Results The list of leading DDDs was glucosidase inhibitor, which accounted for 31.13 % of the total, followed by biguanides which accounted for 21.49%. Conclusions The glueosidase inhibitor, a new type oral hypoglycemic drugs used for lowering postprandial blood sugar level showed remarkable efficacy and safety, so it is currently the first choice for patients with diabetes.

Objective To study the effect of rosuvastatin(RSVT) on the vascular endothelial function(VEF) ,inflammatory fac‐tors(IF) and prognosis in patients with acute coronary syndrome (ACS) after percutaneous coronary intervention(PCI) .Methods Eighty cases of ACS patients with PCI in our hospital selected from July 2010 to July 2013 were randomly divided into observed group and control group ,40 cases in each group .The observation group were given interference treatment with RSVT while the control group received conventional treatment .The VEF ,IF and prognosis were compared between groups .Results The VWF at 4 weeks after PCI in observation group was lower than that in control group [(92 .6 ± 12 .3)% vs .(105 .4 ± 13 .6)% ,P<0 .05];The ET‐1 at 4 weeks after PCI in observation group was lower than that in control group[(55 .6 ± 5 .6)ng/L vs .(67 .8 ± 7 .4)ng/L ,P<0 .05] .The NO at 4 weeks after PCI in observation group was higher than that of control group[(78 .6 ± 9 .4)μmol/L vs .(63 .2 ± 9 .5)μmol/L ,P<0 .05] .The CRP at 4 weeks after PCI in observation group was lower than that in control group[(5 .4 ± 2 .2) mg/L vs .(10 .5 ± 4 .1)mg/L ,P<0 .05] .The incidence of CVE was 5 .00% (2/40) ,restenosis rate was 2 .50% (1/40) in observa‐tion group ,which was significantly lower than those of the control group [30 .00% (12/40) ,10 .00% (4/40)] ,the differences were statistically significant(P<0 .05) .Conclusion RSVT could effectively improve the VEF and reduce inflammation ,CVE and rest‐enosis rate in patients with ACS after PCI .

Objective:To explore the relationship between non-dipper heart rate (HR)and severity of cerebral em- bolism from atrial fibrillation in aged patients.Methods:A total of 95 aged patients with persistent atrial fibrillation complicated cerebral embolism received 24h Holter monitoring within 24h after hospitalization.According to 24h Holter monitoring results,patients were divided into dipper HR group (n=21),non-dipper HR group (n=56) and reverse dipper HR group (n=18).United States national institutes of health stroke score (NIHSS)was used to evaluate neurological deficit severity of three groups,and the relationship between HR and NIHSS score was retro- spectively analyzed.Results:Compared with dipper HR group,there were significant rise in mean HR [(83.09± 8.06)beats/min vs.(89.09±7.83)beats/min vs.(95.50±8.06)beats/min]and NIHSS score [(5.14±2.65) scores vs.(7.95±5.30)scores vs.(11.11±7.41)scores]in non-dipper HR group and reverse dipper HR group, and those of reverse dipper HR group were significantly higher than those of non-dipper HR group,P<0.05 or<0.01.Conclusion:Abnormal circadian changes of heart rate can aggravate patient's condition in aged patients with atrial fibrillation complicated cerebral embolism.So based on routine treatment,regulation of circadian rhythm of heart rate should also be noticed.

Objective:To establish an HS-GC method for the determination of menthol in Xiao ’ er Fufang Mahuangjian Jiegeng syrups. Methods:An Agilent DB-WAX column(30 m × 0. 53 mm,1. 0 μm)with an FID detector was used;the inlet temperature was 150℃, the detector temperature was 250℃;the flow rate of the carrier gas ( N2 ) was 2. 0 ml·min-1 , the split ratio was 1∶1; the headspace equilibrium temperature was 80℃, the balance time was 30 min, and the sample volume was 1ml. Results:The calibration curve of menthol was within the range of 2. 09-20. 92 μg·ml-1, which showed a good linear relationship (r=0. 999 1). The average recovery of menthol was 98. 0%(RSD=1. 8%, n=6). Conclusion: The method is simple, accurate and reproducible, and can be used for the determination of menthol in Xiao’ er Fufang Mahuangjian Jiegeng syrups.

Objective To explore the nursing effect evaluation of vacuum sealing drainage based latissimus dorsi bridge free skin flap to repair refractory wound. Methods Thirty-seven cases of patients with intractable wounds were chosen as the observe group from January 2009 to January 2012, and 26 cases accepting the traditional way of wound care with intractable wounds were selected as control group from January 2006 to December 2008. Control group adopt conventional methods wound and the observation group accepted VSD accessories line wound negative pressure closed drainage before the wound phase 2 latissimus dorsi bridge free skin flap repairment. After treatment, the dressing time, interval and dressing change, the time of hospitalization were observed, and the nursing effect were compared after skin flap to repair for 8 days and 16 days between patients of two groups. Results The dressing time and hospitalization days in observation group after treatment were significantly less than that in control group ( <0.05), the number of dressing have significantly shortened compared with control group ( <0.01), and the dressing change interval in control group had significantly difference ( <0.01) . The effect of 2 patients in control group after skin flap to repair was poorer, but the observation group did not appear significant necrosis. Compared the good rate of two groups, the observation group patients was significantly higher than control group ( <0.01) . The therapy good rate of observation group was significantly better than that of control group (<0.01) . Conclusion The negative pressure closed drainage based ascending latissimus dorsi bridge free skin flap repairment has contributed to cure the refractory wound recovery significantly.

The concept of “disease prevention theory” contains a wealth of preventive medicine thought. Pre-ventive health care service in Traditional Chinese Medicine( TCM) is an important measure for enhancing health qual-ity and improving the quality of living. This contributes to the building of a harmonious society and suits the changing medical model. Despite a series of achievements, there are also some problems in preventative health care service. Based on an analysis of influencing factors on policy implementation, the problems are discussed in the paper. The study provides some suggestions on preventive health care services, including the perfection of operations and man-agement mechanisms, the improvement of awareness on preventive health care service, the strengthening of the talent team, an increase in funding, and an evaluation of policy implementation.