Objective:To evaluate the synergism ofrecombinant M.smegmatis(MS)encoding human granulysin and murine IL-12 to anti-tuberculosis chemotherapy in murine M.tuberculosis M.tuberculosis infection.Methods:Balb/c mice were infected with normal saline, recombinant MS,INH(Isoniazid)+PZA(pyrazinamide),recombinant MS+INH+PZA respectively.The numbers of viable bacteria in the lung and the spleen were counted.The levels of IL12 and IFN-?in serum and IFN-?and TNF-?released from spleen lymphocytes stimulated with PPD were detected with ELISA.Lungs and spleens were prepared for pathological analysis.Results:The recombinant MS group,INH+PZA group,and recombinant MS+INH+PZA group showed significantly reduced number of colony forming units(log CFU/g) compared with saline group.The increase of IL-12 and IFN-?in serum were found in recombinant MS group.IFN-?and TNF-?released from spleen lymphocytes stimulated with PPD in recombinant MS group was higher than that in other groups(P

Objective To explore the clinical significance of the adenosine deaminase ( ADA) and the pro-calcitonin(PCT) in adult patients with active pulmonary tuberculosis .Methods 81 patients with active pulmonary tuberculosis were enrolled into A group ,43 patients with non-tuberculous lung infection were enrolled into B group and 27 patients with lung cancer were enrolled into C group .Serum ADA and PCT were detected .Tuberculosis patients were treated for 6 months of anti-tuberculosis treatment .Results There was a significant difference between group A and B or C group about ADA (t=12.983,10.630,all P<0.01),B and C groups showed no significant difference (t=0.144,P=0.885).Group A PCT compared with group B were significantly different (t=16.775,P<0.01), the A group and C group showed no significant difference (t=1.571,P=0.119),group B compared with group C were significantly different (t=11.235,P<0.01).Tuberculosis treatment remission in 67 cases,no relief in 14 ca-ses.The levels of serum ADA and PCT in remission were significantly lower than in patients with no remission group (t=3.087,3.404,all P<0.05).Conclusion The serum ADA can be used as diagnostic indicators of active tuber-culosis,ADA and PCT joint determination can determine the prognosis of active tuberculosis .

The application of semiconductor laser in medicine has extended rapidly with the development of its series techniques. Semiconductor laser has its own advantages, such as small body, light weight, low price and optional wavelengths. The application of semiconductor laser is limited within two fields, which covers other lasers' application fields. One is the utilization of its optic property for tissue detection and disease diagnosis, and the other is the utilization of its biological effect for disease therapy. The two kinds of application are both specified in this paper.

By reviewing experiment teaching,the primal problems and solutions of practical training in preventive medicine specialty are explored in order to provide some beneficial reference and help for experimental teaching.

Objective:To develop a domain-specific perfectionism scale for college students.Methods:Exploratory and confirmatory factor analytic approaches were used to develop and evaluate the intra-structure of the scale.Results:Exploratory principle factor analysis of the items indicated that the scale had five factors,explaining 57.538% of the total variance.Internal consistency analysis showed that the Cronbach's ? coefficient,split-half and test-retest correlation coefficients were 0.648～0.781,0.664～0.790,0.720～0.779 respectively for the factors.Confirmatory fator analysis showed that the fit indexes for ?2/df,GFI,AGFI,NFI,NNFI,RMSEA,CFI,IFI were 2.32,0.91,0.88,0.84,0.88,0.06,0.90,0.90.Conclusion:Psychometric properties analysis shows that the scale achieves from good to exellent levels of internal consistency,test-retest rliability and content,construct and criteria-related validity.

Objective To analysis the effect of high filtration rate continuous renal replacement therapy in severe acute pancreatitis pa-tients. Methods According to the admission time,56 severe acute pancreatitis patients were divided into the observation group ( from Jan. 2012 to Mar. 2013,26 cases) and the control group (from Apr. 2010 to Dec. 2011,30 cases). The control group was given CRRT for hemopu-rification while the observation group was given high filtration rate CRRT. The vital signs of rescue period,treatment effect,inflammatory medi-um level,fatality rate and prognosis of the two groups were compared. Results There was no significant change in terms of temperature after operation in the two groups. Pulse and breathing in the observation group were lower than that of the control group with a significant difference (P<0. 05). APACHEⅡ and ALT of the observation group were lower than that of the control group 24 h after the operation,and the differ-ence is of statistical significance(P<0. 05). Conclusion It would get a satisfactory effect to use high filtration rate CRRT in severe acute pancreatitis patients since it can obtain better treatment effect with more stable vital signs, lower inflammatory medium level and lower fatality rate.

ObjectiveTo examine the quality of Chinese herbal pieces Traditional Chinese Herbal Medicine (Chinese Herbal Pieces) in Guasu Province during 2010 to 2012.MethodsBy using the examination method according to selective examination requirements, 15 varieties of 865 batches of Chinese herbal medicine from 14 regions in Gansu Province during 2010 to 2012 were examined. Evaluation was conducted from the unqualified items and sample quality.Results Among 15 varieties of 865 batches of Chinese herbal medicine from Tibetan Autonomous Prefecture of Gannan in Gansu Province, 240 batches were unqualified, including 99 batches of fake, 104 batches of adulteration, the total fraction defective was 30.1%.ConclusionThe problems in quality of Traditional Chinese Herbal Medicine (Chinese Herbal Pieces) in Gansu Province are serious. Therefore, TCM market inspection and management should be reinforced and the quality of Chinese herbal medicine should be improved as soon as possible.

Extended-field and subtotal nodal radiation therapy (RT),developed in the 1960 s,was first reliably curative treatment for early-stage Hodgkin lymphoma (HL). However, the large volume of normal tissue irradiated resulted in significant delayed toxicity, including cardiac disease and second cancers (SC).The 30-year cumulative incidence of heart disease among adult survivors receiving 40-45 Gy of extended-field or mantle RT is approximately 30 ％; the incidence of SC is similar. How to improve disease control while reducing the toxicity of treatment has been a major objective of HL trials. Contemporary involved-field RT (IFRT) and involved-node RT (INRT) reduces irradiated volumes and produces significant reductions in normal tissue dose, and response-adapted therapy may be a means of identifying those patients most likely to benefit from treatment reduction or intensification, and enhanced screening will facilitate early intervention to reduce the clinical burden of late effects. There is a increasing interest in elucidating the genetic correlation of treatment toxicity.

OBJECTIVE:To prepare zolmitriptan-diclofenac microemulsion,and conduct quality evaluation and in vitro trans-dermal study. METHODS:Using solubility and microemulsion area in pseudo-ternary phase diagram as indexes,the types of oil phase and mixed emulsifier ratio of zolmitriptan-diclofenac microemulsion were screened;the microemulsion quality was inspected using particle size,Zeta potential,appearance and stability. HPLC was used to measure the contents of zolmitriptan and diclofenac. Transdermal diffusion test instrument was used,2 g microemulsion was smeared in cuticle of extracouporeal rats'skin,and cumula-tive transdermal rate in 24 h was determined. RESULTS:The microemulsion formulation was as follow as 10% oil phase(octanoic acid triglyceride),25% mixture emulgator [polysorbate 80-brij 97 (1:1)],8.3% propylene glycol and 25 mg zolmitriptan,1.25 mg diclofenac,and water adding to 100 mL. The average particle size of prepared microemulsion was(28.2±2.5)nm,Zeta poten-tial was(-3.25±0.33)mV,the appearance was rounding;the microemulsion showed no stratification or flocculation at room tem-perature after placed for 1 month. Contents of zolmitriptan and diclofenac were 0.248 mg/mL,12.46 mg/mL(n=3);24 h cumula-tive transdermal rates were 80%,75%. CONCLUSIONS:Zolmitriptan-diclofenac microemulsion is prepared,and its in vitro trans-dermal ability is good.

Objective To explore the mental state of family members of neurosis patients, and to observe the effect of psychological intervention on these people. Methods The families of neurosis patients were randomly divided into experimental group (psychological intervention) and control group (no psychological intervention). Group or individual psychological consultations were performed in experimental group, and the intervention effect was evaluated with self-rating anxiety scale (SAS) scores method and was compared between experimental group and control group. Results The SAS score was 38.84?5.34 before psychological intervention and 29.43?2.99 after psychological intervention, respectively, and it had significant differences between before and after psychological intervention treatment, but no significant difference in control group. The effect of psychological intervention was correlated with age, education condition, occupation and habitation. Conclusion Mental problems exists in the family members of neurosis inpatients, and the psychological intervention can improve their psychological obstacle and ease anxiety, which benefits not only to the relatives' health but also to the neurosis patients' therapy and restoration.