Objective To study the pathogenic molecular mechanism of H.pylori through analyzing its effect of on MMP-9 expression. Methods BGC-823 cells were cocultured with ATCC49503(CagA +)and HP 030811 (CagA -) H.pylori strains respectively, then the total cellelar RNA was extracted. The MMP-9 mRNA expression in BGC-823 cells, 9 cases of H.pylori-positive and 9 cases of H.pylori-negative gastric ulcer biopsy tissues was detected by RT-PCR. The MMP-9 protein expression on the paraffin-imbedded tissue sections of 17 cases of H.pylori-positive and 14 cases of H.pylori-negative gastric ulcer tissues was detected with immunohistochemical technique, and was quantified by image quantitative analysis. Results H.pylori induced MMP-9 expression in BGC-823, and the level of MMP-9 expression induced by CagA -postive H.pylori strain was higher than that by CagA -negative one. MMP-9 expression level was higher in the H.pylori-positive gastric ulcer tissues than that in the H.pylori-negative ones. Conclusion H.pylori-induced MMP-9 expression may play an important role in gastric ulcer formation and gastric carcinogenesis.

Objective:To analyze influence of vaginal hysteroscopy and laparoscopic surgery on tubal pregnancy patients’ surgical outcome and inflammatory stress response.Methods: Chosen 86 cases of tubal pregnancy patients between July 2012 to July 2013 in our hospital undergoing surgery as study object, according to a random number table divided into hysteroscopy group (43 cases) and laparoscopic group (43 cases), compared surgical outcomes, perioperative differences in the level of inflammation and stress level.Results: No significant difference between hysteroscopy group and laparoscopic group on surgical success rate and ipsilateral tubal patency statistically(x2=0.241,x2=0.193;P>0.05), postoperative intrauterine pregnancy rate was higher than laparoscopic group, again ectopic pregnancy rate was less than laparoscopic patients(x2=7.294,x2=8.283;P<0.05); hysteroscopic surgery group E, Cor, IL-2, IL-6, IL-8, CRP levels were lower than laparoscopic group(t=6.842,t=6.283,t=8.536,t=6.106,t=6.283,t=6.376;P<0.05),TSH levels was higher than laparoscopic group(t=6.823,P<0.05).Conclusion:Vaginal hysteroscopy and laparoscopy can be used for the treatment of tubal pregnancy, hysteroscopy has an advantage in improving patient again intrauterine pregnancy, reduce postoperative systemic stress and inflammation levels, and promote the recovery of patients after surgery respect.

Objective: To analyze the effect of single-hole thoracoscopic surgical treatment on elderly spontaneous pneumothorax patients’ postoperative assessment and serological markers and explore single-hole thoracoscopic surgery application. Methods:78 cases of elderly patients with spontaneous pneumothorax were retrospectively analyzed, and divided into single-hole thoracoscopy group with 35 patients and conventional thoracoscopic group with 43 patients. Then the surgery indicators, postoperative pain scores and perioperative serum target level differences were compared between the two groups. Results: The operative time, postoperative drainage time and total hospital stays of single-hole thoracoscopic surgery patients were shorter than that of conventional thoracoscopic group. The differences were statistically significant (t=7.183, t=5.294, t=6.938;P<0.05). The blood loss and postoperative drainage were less. The differences were statistically significant (t=6.883, t=8.735; P<0.05). The postoperative pain scores within one week was less than conventional thoracoscopic group. The differences were also statistically significant (t=6.882, t=5.724, t=5.284;P<0.05). The serological indicators, CRP, IL-1, TNF-α,α1-AT levels of patients in the single-hole thoracoscopy group were lower than the conventional group (t=8.293, t=7.274, t=9.284, t=7.183;P<0.05). Conclusion:VATS and conventional hole thoracoscopic surgery can be used for treatment of spontaneous pneumothorax in elderly, but single-hole thoracoscopy has more advantages in reducing surgical trauma and postoperative pain and optimize systemic inflammation associated factors.

Tachycardia induced cardiomyopathy is a kind of cardiomyopathy caused by sustained or recurrent tachycardia.Various types of tachyarrhythmias can cause TIC, and the main clinical manifestations of it are cardiac dilatation and cardiac dysfunction.TIC can occur at all ages and is a reversible and acquired cardiomyopathy with a generally benign prognosis.After early diagnosis and reasonable treatment to relive the tachycardia, the function of the heart can partially or completely recover.In clinical practice, pediatricians do not fully understand the disease, as a result, it is easily missed, misdiagnosed, then the proper diagnosis and treatment will be delayed.This article provides a systematic overview of the pathogenesis, clinical characteristics, diagnosis, differential diagnosis, treatment and prognosis of TIC in children, and aims to provide a basis for clinical pediatricians to recognize it early and treat it promptly.

The expression of transforming growth factor-beta1 (TGF-beta1) in placental tissue of pregnancy-induced hypertension (PIH) and the relationship between the level of expression of TGF-beta1 and the amount of vascular cell adhesion molecule-1 (VCAM-1) in serum was studied. Immunohistochemistry ABC was used to detect the expression and distribution of TGF-beta1 in placental tissues in 40 PIH women and 20 normal pregnancy women. High resolution pathological image analysis system was used to determine the quality of TGF-beta1. The VCAM-1 in serum was examined by enzyme linked immunoabsorbent assay (ELISA). The results showed that TGF-beta1 could be express in syncytiotrophoblast. The levels of TGF-beta1 expression in placental tissues of the patients with moderate and severe PIH were significantly higher (P < 0.05), while the serum VCAM-1 was significantly lower than in normal group (P < 0.01). There was a significant positive correlation between the expression of TGF-beta1 in placental tissues and the serum VCAM-1 (r = 0.969, P < 0.01). It was concluded that the level of TGF-beta1 expression in PIH was increased and was positively correlated with the amount of serum VCAM-1, indicating that they might be involved in the pathogenesis of PIH.
Hypertension, Pregnancy-Induced/*metabolism ; Placenta/metabolism ; Transforming Growth Factor beta/*metabolism ; Transforming Growth Factor beta1 ; Vascular Cell Adhesion Molecule-1/*blood

Sterols are one of the active classes of compounds in Inonotus obliquus for their effective therapy of many diseases. In field environment, this fungus accumulates large amount of sterols. In cultured mycelia, however, this class of compounds is less accumulated. For analyzing the factors responsible for differing sterol composition, the field-grown and cultured mycelia were extracted with 80% ethanol at room temperature and total sterols were prepared using silicon gel column chromatography followed by identification using either GC-MS or spectroscopic methods. For culturing Inonotus obliquus, the seed culture was grown either in basic medium consisting of glucose (2%), yeast extract (0.5%), KH2PO4 (0.01%), MgSO4·7H2O (0.05%) and distilled water at pH 6.5, or the basic medium supplemented with serial concentrations of AgNO3. The results indicated that field-grown mycelia contained lanosterol and inotodiol (comprised 45.47% and 25.36% of the total sterols, respectively) and other 10 sterols (comprising the remaining 30.17%) including ergosterol biosynthetic intermediates such as 24-methylene dihydrolanosterol, 4,4-dimethylfecosterol, 4-methyl fecosterol, fecosterol and episterol. Column chromatography also led to the isolation of lanosterol, Inotodiol, trametenolic acid, foscoparianol B and a new triterpenoid foscoparianol D in field-grown mycelia. In comparison, the cultured mycelia only contained three sterols with ergosterol as the predominant one (82.20%). Lanosterol only accounted for 3.68%. Supplementing Ag+ into the culture at 0.28 μmol·L-1 greatly enhanced content of lanosterol (accounting for 56.81%) and decreased the content of ergosterol (18.5%) together with the presence of intermediates for ergosterol biosynthesis. These results suggested that the sterol composition in mycelia of the fungus can be diversified by supplementing substances inhibiting enzymatic process towards the synthesis of ergosterol. Harsh growth conditions in field environment (I.e. temperature variation, UV irradiation etc.) can delay the synthesis of ergosterol and hereby diversify the sterol composition in the mycelia of Inonotus obliquus.

Background and purpose:The membrane cytoskeletal crosslinker—Ezrin may be involved in several functions including cell adhesion,motility and cell survival.There is increasing evidence that it regulates tumor progression.However,the role of Ezrin in the carcinogenesis,progression and metastasis of primary sporadic colorectal carcinoma is still under investigation.This research is to study the expression,the localization and the clinical significance of Ezrin in human sporadic colorectal carcinoma(SCRC).Methods:Immunohistological EnVision staining was used to detect the expression of Ezrin in 132 cases of human sporadic colorectal carcinoma and 43 adjacent normal colorectal mucosa,and the different expressions of Ezrin among metastatic and non-metastatic SCRC,including 74 metastatic cases and 58 non-metastatic cases were also under investigation.Results:① The expression rate of Ezrin in SCRC was significantly higher than that in adjacent normal colorectal mucosa(79.5% Vs 11.6%,P

Objective To study the activities of the alcoholic extract from Fructus Polygoni Orientalis (EFPO) against antioxidation. Methods The peroxidation in homogenate of rat heart, liver and kidney was induced by ?OH generation system Fe2++ascorbic acid. Malondialdehyde (MDA) was determined by TBA colorimetric method. Superoxide (O-2) from zymosan-stimulated neutrophils of rat was measured by NBT reduction method. H2O2-caused hemolysis of erythrocytes was measured by spectrometry. Results MDA in homogenate of heart, liver and kidney of rat that induced by ?OH generation system Fe2++ascorbic acid was inhibited by 6.3, 12.5, 25, 50, 100, 200 ?g/L EFPO; IC50 of EFPO to MDA in heart, liver and kidney was 31.8, 32.5 and 40.2 ?g/L respectively and its dose-effect relation was of negative relativity and the relative coefficient was -0.886, -0.874 and -0.918 (all P

The expression of endothelial nitric oxide synthase traffic inducer (NOSTRIN) was examined in the umbilical vessels of the patients with pre-eclampsia (PE) to explore its possible role in the pathogenesis of PE. The NOSTRIN mRNA in umbilical tissues was determined by RT-PCR. The eNOS activity in umbilical vessels was spectrophotometrically detected. NO2-/NO3-, the stable metabolic end products of NO, was measured by using nitrate reductase. RT-PCR showed that the expression level of NOSTRIN was significantly higher in women with PE than in the normal group (P<0.01). The activity of eNOS was significantly decreased in PE group [(12.83+/-3.61) U/mg] than in normal group [(21.72+/-3.83) U/mg] (P<0.01). The level of NO2-/NO3- in PE patients (27.53+/-7.48) micromol/mg was significantly lower than that of normal group (54.27+/-9.53) micromol/mg (P<0.01). The significant negative correlation existed between the expression of NOSTRIN and the activity of eNOS in umbilical vessels of women with PE (r=-0.58, P<0.01). It was concluded that the level of NOSTRIN expression was increased in umbilical vessel of women with PE, indicating that it may be involved in the pathogenesis of PE.
Intracellular Signaling Peptides and Proteins/genetics ; Intracellular Signaling Peptides and Proteins/*metabolism ; Pre-Eclampsia/*enzymology ; Pre-Eclampsia/etiology ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; Umbilical Arteries/cytology ; Umbilical Arteries/*enzymology ; Umbilical Veins/cytology ; Umbilical Veins/*enzymology