Objective To investigate the diagnostic value of ultrasonic elastic strain rate and conventional ultrasound for thyroid nodules,and to guide the clinical treatment.Methods 72 cases,a total of 86 nodules,were treated with ultrasound examination for thyroid nodules and operation were given conventional and ultrasonic elastic strain rate echocardiography before operation, after operation they had a pathological examination for tubercle.The accuracy,specificity and sensitivity of ultrasonic elastic strain rate combined with ultrasound to check the benign and malignant thyroid nodules were analyzed.Results The accuracy of ultrasonic elastic strain rate combined with con-ventional ultrasound was 93.41%,which was significantly higher than 73.67%of the conventional ultrasound (χ2 =12.0,P<0.05) .Conclusion Ultrasound elasticity strain rate combined with conventional ultrasound can effectively differentiate benign and malignant thyroid nodules and improve the accuracy of diagnosis of malignant thyroid nodules, which is conducive to the treatment and prognosis.

Although osteoporosis is often considered asilent disease,it is highly prevalent.It will definately affect the health of elderly people and bring heavy burden to the society.Studies of osteoporosis will also draw great attention in the field of geriatrics.The diagnosis,treatment,and monitoring of osteoporosis have not yet been standardized.Multidisciplinary medical staff should work together along with the cooperation of the patients.

OBJECTIVE:To promote safe,effective,economical and proper medication.METHODS:Prescriptions in the outpatient and emergency departments of our hospital in2003were randomly selected;the irrational prescriptions were ana?lyzed with regard to drug descriptions,pharmacological knowledge and literatures.RESULTS:The irrational one amounted to4.92%of the total selected prescriptions;antibiotics drug abuses amounted to84.12%of the irrational ones.The irrational drug use problems were mainly seen in administration scheme,repeated dosage,menstruum,administration,drug combina?tion,compatibility and combined toxicity.CONCLUSIONS:Clinical pharmacist plays an important role in promoting rational drug use by checking prescriptions.

0.05);The expression of Survivin in hilar cholangiocarcinoma was closely statistically related with the expressions of PTEN and nm23.Conclusion:The expression of Survivin in hilar cholangiocarcinoma is significantly higher than that in tissues near carcinoma,suggesting its correlation with tumor;Survivin plays an important role in the oncogenesis and metastasis of hilar cholangiocarcinoma;Survivin is statistically related with PTEN and nm23 and detected together may helpful for diagnosis and prognosis of hilar cholangiocarcinoma.

AIM: To study factors influencing mannitol to induce blood brain barrier (BBB) opening. METHODS: By constant flow pump, mannitol with different concentration (20% and 25%) was infused into left internal carotid artery of adult Wistar rats via their left external carotid artery at different speed and time. Firstly, after mannitol with a concentration of 25% was transfused, the optimal open time of BBB was investigated using Evans blue as an indicator by injection at different time (0, 2, 4, 6, and 8 min). Then influences of concentration of mannitol, speed of infusion, and change of time on mannitol to induce BBB opening were studied. Finally, death rate was counted according to sex and body weight in order to observe the influence of sex and body weight on tolerance of rats. RESULTS: The open degree of BBB was optimal after 2 min when 3.6 ml mannitol with a concentration of 25% was infused in 30 s at a speed of 0.12 ml?s -1 . However, mannitol with a concentration of 20 % did not almost have any effect on opening of BBB. Slowing of infusion speed or shortening of infusion time of mannitol reduced opening degree of BBB. Body weight affected rat tolerance to mannitol, and the tolerance of the rat was higher in those with higher weight. CONCLUSION: The factors influencing mannitol to induce BBB opening are as follows: concentration, infusion speed, and keeping time of mannitol. And the tolerance to mannitol is not concerned with the sex of the rats, but the body weight of the rats.

Objective To study the expression of DNA methyltransferase 1 (DNMT1) in human cervical carcinoma tissue and cells, analyze the relationship between the expression level of DNMT1 and the clinicopathologic features of cervical carcinoma, and explore the role DNMT1 plays in tumorigenesis of cervical carcinoma. Methods The expression levels of DNMT1 protein in 3 cervical cancer cell lines, 89 samples of cervial cancer tissue, 20 samples of cervical intraepithelial neoplasia (CIN) and 20 samples of normal cervix were determined by immunohistochemistry method. The expression levels of DNMT1 mRNA in all the cell lines and 79 paraffin sections of cervix tissue were determined by RT-PCR. Results The DNMT1 protein was expressed in all the three cervix cancer cell lines. Positive expression was detected in 71.9% of cervix cancer samples, 35.0% of CIN samples and 10.0% of normal cervix samples, with significant differences among the three groups (P

Objective To investigate the elationship between the expression of Cathepsin D and Laminin receptors with the invasion and metastasis of gastric cancers.Methods Cathepsin D and Laminin receptors immunoreactivities were evaluated in 96 patients with gastric cancer using streptavaclin pemxidase method.Results Over expression of Cathepsin D in gastric cancer tissue was significantly related with the depth of invasion,lymphnode metastasis and grade of differentiation (P<0.01,P<0.01 and P<0.05, respectively).Moreover,the high expression of Lamninin receptors in gastric cancer had significant correlations with the invasion depth and grade of differentiation(P<0.05 and P<0.01);The positive expression of Laminin receptors were not significantly related with lymph node metastasis(P>0.05).By statistics analysis Cathepsin D had a significant positive correlation with Laminin receptors (P<0.05). Conclusion Cathepsin D and Laminin Receptors play all important role in the invasion and metastasis of gastric Cancers.

Objective To investigate the expressions and significance of cyclinD1 in oral squamous cell carcinoma (OSCC). Methods The published studies were searched in the Cochrane Library,Embase,PubMed,CNKI,VIP,Wanfang and CBM databas-es,the quality of the included studies was assessed.Revman5.0.1 software was adopted to test for heterogeneity of the selected re-search literature and analyze the correlation of cyclinD1 and OSCC by calculating OR and 95%CI .Results A total of 31 case-con-trol studies were included in the study,the case group (OSCC group)was 1 604 cases and the control group was 864 cases (inclu-ding normal oral mucosa tissue 452 cases and precancerous lesions 412 cases).The Meta analysis of results showed that the expres-sion of CyclinD1 in oral squamous cell carcinoma was significantly higher than normal tissue[OR (95%CI )at 11.92 (6.71 -21.18)].CyclinD1 was related to differentiated degree,lymph node metastasis and clinical stage of OSCC positively(P <0.05),but was not related to age and gender (P >0.05).Conclusion According to the current relevant research,the over expression of cy-clinD1 is closely related to occurrence and development of OSCC,which might be valuable for the early diagnosis,treatment,and prognosis of OSCC.

Studies have shown that microRNA plays a role of oncogene or tumor suppressor gene in the carcinogenesis and progression of tumor.However, the role of microRNA-143 (miR-143) in esophageal squamous cell carcinoma (ESCC) needs further study.Aims: To investigate the expression and clinical significance of miR-143 in ESCC.Methods: Sixty-three ESCC tissues and corresponding para-cancerous tissues, 40 esophageal intraepithelial neoplasia (IEN) tissues and corresponding normal tissues from Jan.2013 to Dec.2013 at the First Affiliated Hospital with Nanjing Medical University were enrolled.The expression of miR-143 was examined by real-time quantitative PCR, and its correlations with clinicopathological features were analyzed.Results: Compared with controls, expression of miR-143 was down-regulated in ESCC and IEN (P<0.05).Expression of miR-143 was correlated with pathological type (P<0.001), but not with gender and age (P>0.05).Expression of miR-143 was correlated with pathological staging, lymph node metastasis and TNM staging (P<0.05), but not correlated with tumor infiltration depth in ESCC patients (P>0.05).Conclusions: Expression of miR-143 is down-regulated in ESCC and IEN tissues, which may be closely related to the development and progression of ESCC, and has the potential to be used as a new target for diagnosis of ESCC.