0.05). - 40 mV activated threshold potential and - 30 mV peak potential for control group respectively droppedto - 60 mV and - 40 mV after irradiating 7 min. The half-activation voltage and the slope factor of the activationcurves of Na+ channel were also changed by the laser's exposure. The former changed from (- 42.091 ?1.537) mVto (54.971 ?1.846) mV (n= 8, P

Purpose: A kind of sine wave magnetic field generator with the extremely low frequency (ELF) is developed, the frequency and intensity of the magnetic field can be adjusted, and has been easily used in Bio-experimental study. Methods: At first ,we design a DC power supply which provide DC voltage to other chips,then the signal generated by single chip ma-chine system and the power amplifier combined by discrete components, it provides current 0 mA～136 mA.The current coil is designed by experiment's required,the size or turns is changed, the magnetic field intensity is changed too. Results: The fre-quency of the device output is 0 kHz-25 kHz with the intensity in the range of 0 mT～10 mT within which the continuous lin-earity is adjustable. Power sources are less than 30 W ,which can work for long.This device performs reliably and the measured values in accord with the theoretical values and has been used in the experiment of magnetic biological effect. Conclusions: The experiment results indicate that there exist the effects of ELF sine wave magnetic field upon potassium current of neurons, and that such bioeffects vary with the intensity and frequency of the field.Also,there may exist window effect areas.

Objective To investigate the situation of family intimacy and adaptability, and social avoidance and distress in severe burn patients, and explore the correlation between them, so as to provide basis for intervention. Methods From January, 2013 to June, 2015, 289 patients with severe burn were surveyed with self-designed general condition questionnaire, Family Adaptability and Cohesion Evaluation Scale (FACES Ⅱ) and Social Avoidance and Distress Scale (SADS), and analyed with Pearson correlation analysis. Results The real family intimacy and adaptability were lower in the severe burn patients than in national norm (t>3.830, P<0.01), and the ideal family intimacy, dis-satisfied intimacy level, ideal adaptability and dissatisfied adaptability level were higher in the severe burn patients than in national norm (t>3.857, P<0.01). The SADS score was higher in the severe burn patients than in national norm. The real intimacy, real adaptability and the to-tal score of FACES II negatively correlated with the scores of SADS (P<0.05), while the levels of dissatisfied intimacy and adaptability posi-tively correlated (P<0.05). Conclusion The social avoidance and distress exist in severe burn patients, which may associated with the family intimacy and adaptability, that some interventions may target to.

Objective: We studied the differences in intelligence,self-concept and behavior problem between girls with true precocious puberty and the normal girls.Methods: 35 girls with true precocious puberty aged 8 to 10 years was put into the research group;another 35 girls was put into the normal group(n=35).Both the research group and normal group completed the Piers-Harris Children's Self-concept Scale(PHCSS) and Raven's Standard Progressive Matrices in order to evaluate their levels of self-concept and intelligence respectively.Their parents completed a Child Behavior Check-list(CBCL) and a questionnaire on background status.Results: ①Children in the research group had significantly higher intellectual level than those in the control group.②The PHCSS yielded significantly lower scores of total self-concept,behavior and somatic appearance in the research group than those of the control group.③Higher scores of internal behavior problems,external behavior problems,depression,poor social communication,somatic complaints,delinquent behavior and aggressive behavior at CBCL showed in the research group than those of the control group.Conclusion: ①The level of intelligence of the children in research group was higher than that of children in the control group.②Girls with true precocious puberty showed more behavior problems and lower level of self-concept than those in the control group.

Objective To explore the application value of PBL combined with medical simulation training in pediatric clinical practice for long-schooling program medical students.Methods Totally 41 long-schooling program medical students in pediatric department were taken as study group while 40 medical students as control group.PBL combined with medical simulation training was used in study group while traditional method was used in control group.Teaching effect was evaluated using score analysis,PBL learning performance rating scale and student questionnaire.Results Scores of theory test and clinical skill exam after course completion in study group were significantly higher than those of control group (P ＜ 0.01).PBL learning performance rating scale and student questionnaire showed that the student's comprehensive ability has significantly improved.Conclusions Application of PBL and medical simulation training in the pediatric clinical practice for long-schooling program medical students can enhance students' problem analyzing and summarizing abilities,problem-solving ability and practical operation ability.It can effectively cultivate team collaboration and doctor-patient communication skills as well as improve the quality of teaching.

Objective To observe the effect of Wnt/β-catenin pathway on proliferation of human umbilical vein endothelial cell (HUVEC) using the glycogen synthase kinase 3β (GSK-3β)-targeting RNAi recombinant adenovirus vector. Methods Homologous recombination and cloning techniques were used to construct RNAi recombinant adenoviral expressive vectors specific to GSK-3β. Then, the adenovirus plasmids was transfected into HEK 293A cells to produce adenovirus and amplify the adenoviral stock. Plaque forming assay was used to titer the adenoviral stock. The GSK-3β and β-catenin protein expressions were detected by Western blot and immunohistochemistry. The proliferation of HUVEC was detected with MTr assay. Results The RNAi adenovirus vectors specific to GSK-3β were successfully produced with high titer. The expression of GSK-3β protein in HUVEC could be down-regulated efficiently by the RNAi adenovirus, along with increased β-catenin protein expression. The proliferation of HUVEC was significantly increased (P < 0.05 or P < 0.01) after infected with GSK-3β RNAi recombinant adenovirus for 3, 5, 7 days. Conclusion RNAi adenovirus is an important tool that can inhibit the expression of GSK-3β efficiently, along with increased β-catenin protein expression. Up-regulating of the Wnt/β-catenin pathway might play an important role in the proliferation of HUVEC.

The standardized training for pediatric residents is a necessary route to promote their clinical ability and make them grow up.With the aim to improve the residents' basic theory,clinical skills and medical quality,we set up training plans according to the requirement of standardized training in shanghai,earnestly implement the related training in the process of training content,method,time node and stage appraisal system,formulating personalized training plan and implementing one to one teaching.Besides,we attach importance to the cultivation of scientific research and teaching ability of residents and set up a perfect evaluation system.Residents are required to obtain a certificate of training through the unified comprehensive examination before graduation.Here,the problems existing in the process of pediatric resident standardization training and preliminary countermeasures are also discussed.

Purpose To observe the change of renal parenchyma elasticity in patients with chronic glomerulonephritis (CGN), and to explore the value of acoustic radiation force impulse (ARFI) in the assessment of renal histological damages in CGN. Materials and Methods 123 patients with CGN and 27 healthy volunteers were enrolled, CGN patients were divided into three groups according to renal histologic scores: mildly, moderately, and severely impaired. Shear wave velocities (SWV) of the renal parenchyma were measured and compared in different groups, the correlation between the SWV measurements and renal fibrosis Katafuchi scores, serum creatinine (Scr) and estimated glomerular filtration rate (e-GFR) was accessed, and receiver operating characteristic (ROC) curves analyses were also performed to assess the value of ARFI for the diagnosis of pathology impairment degree in CGN. Results There were statistically significant differences in SWV measurements between each CGN patient group and the control group (F=16.592, P<0.01); the mean SWV in patients with severe kidney impairment was significant lower than that of mildly impaired, moderately impaired, and the control groups (P<0.001). SWV measurements correlated significantly with renal fibrosis Katafuchi scores (r=-0.481, P<0.01), Scr (r=-0.441, P<0.01), and e-GFR (r=0.546, P<0.01); ROC analyses indicated that the sensitivity was 63.4%, 71.4%, 93.8%, specificity was 77.8%, 71.3%, 79.9%, and the area under the curve was 0.730, 0.738 and 0.870, when using the optimal cut-off value of 2.65 m/s for the diagnosis of mildly impaired kidneys, 2.50 m/s for moderately impaired kidneys, and 2.34 m/s for severely impaired kidneys, respectively. Conclusion ARFI is expected to become an effective tool for non-invasive evaluating of renal histological fibrosis in CGN patients.

ObjectiveTo study the characteristics in negative energy balance in different-aged patients with metabolic syndrome (MS) and come out with nursing countermeasures.Methods Twenty-four hour dietary data reviewing and the international physical activity questionnaire were used to investigate energy intake and consumption among 384 MS patients.The fat content,fat percentage and lean body mass were measured by the human body composition analyzer.Body mass index (BMI),waist/height,fat percentage,lean body mass,energy intake and energy consumption were compared between different ages and sexes.Results In the group aged over 51 years,the waist/height of female MS patients was significantly smaller than their male counterparts (P<0.05).For all groups of different ages,the percentage of fat in the female patients was significantly larger,their lean body mass was less(P<0.01), their intake of energy was significantly lower than that of the males (P<0.05).The intake of energy among the male patients at the group aged 21~30was significantly larger than the males of other age groups and so it was with the energy intake of the males aged 21-70 years as compared to the males aged 71~80 years (P<0.05).Conclusion Education on disease knowledge should be strengthened among healthy people so as to realize the negative energy balance based on the rate of waist/height,body composition,energy intake and energy consumption,which is of great significance for the prevention and control of metabolic syndromes.

Objective To explore the value of 18F-FDG PET/CT in predicting tumor recurrence for hepatocellular carcinoma (HCC) after liver transplantation (LT).Method We respectively analyzed 52 patients with HCC who underwent the 18F-FDG PET/CT examination before LT.In terms of tumor recurrence,all patients were divided into recurrence group and non-recurrence group.According to the degree of 18F-FDG uptake,all patients were divided into PET(-) group and PET (+) group.The SUVmax of primary tumor/the SUVmax of normal-liver background (T/B) was calculated by 18F FDG PET/CT.All patients were then divided into T/B≤1.15 group and T/B＞1.15 group.Result During the follow-up period,25 out of 52 patients (48.1％) developed posttransplant HCC recurrence and 27 (51.9％) had no recurrence.T/B of patients with recurrence (2.51 ± 0.95) was significantly higher than that of patients with non-recurrence (1.37 ± 0.46),t =4.12,P＜0.001.The disease-free survival rate of 0.5 year,1 year,2 years and 3 years after LT in PET(-) group and PET(+) group was 100.0％,92.3％,92.3％ and 92.3％,and 89.7％,59.0％,43.6％ and 35.7％ respectively.Log-rank test revealed that disease-free survival rate in PET(-) group was significantly higher than that in PET(+) group,x2 =17.8,P=0.003.The disease-free survival rate of 0.5 year,1 year,2 years and 3 years after LT in T/B≤1.15 group and T/B＞1.15 group was 100.0％,92.3％,92.3％ and 92.3％,and 89.5％,59.7％,42.1％ and 33.7％ respectively.Log-rank test showed that disease-free survival rate of T/B≤1.15 group was significantly higher than that of T/B＞1.15 group,x2 =10.24,P =0.001.Conclusion 18F-FDG PET/CT can predict HCC recurrence after LT.PET(-)and T/B≤1.15 of the 18F-FDG PET/CT in patients with HCC after LT were associated with a good prognosis,and PET(+) and T/B＞1.15 of the 18F-FDG PET/CT in patients with HCC after LT with a poor prognosis.