The pathogenesis of cancer-related sarcopenia is complicated,involving all aspects of tumor and the body's metabolism,mainly related to accelerated proteolysis and insufficient protein synthesis.Cancerrelated sarcopenia can predict antineoplastic-induced dose-limiting toxicities,increase complications,and reduce survival rate.The interventions of cancer-related sarcopenia includes nutrition,exercise,hormone therapy and other interventions.A single treatment can not reverse clinical outcome.Various interventions are still in the investigation stage,in which more evidence-based studies are needed.

This article presents the experience ofPhysician-in-charge CHEN Ying-yi in treating maternal-fetal ABO incompatibility from four aspects:cause and pathogenesis ofthe disease, treatment thought, special prescription for the disease alone, and treatment experience.Mrs.CHEN Ying-yi considers the damp, heat and stasis pathogen as key factors ofthe disease, and the deficiency ofthe spleen and the kidney as the fundamental reasons.The principle oftreatment is eliminating pathogenic factors and supporting healthy energy, and treating the disease and preventing abortion.Her empirical prescription of‘lianhuang tang(decoction oflotus receptacle and prepared rhubarb )', modified in accordance with the symptoms, has obtained very good curative effect for the treatment ofthis disease.

purpose:This paper is to provide the gerneral principle and method of using the wedge filter. Materials and Methods:A concept of the irradiation angle and a equation to calculate it were introduced. The radiation angle and the given dose for each field were derived from this equation.The wedge angles of each field were got.Results: By means of the examples of laryngocarcinoma and carcinoma of maxillary sinus,the isodose graphs from the TPS using the radiation angles of the equations comfirmed this method.Conclusion: The way introduced is brief and raliable. It may help to optimize the treatment plan.

Objective To observe the effect of four-handed technique in light cured resin veneer in the treatment of dental fluorosis. Methods Eighty outpatients with dental fluorosis in our hospital from January 2011 to January 2014 were randomly divided into two groups: 40 in the study group were treated with four-handed technique and another 40 in the control group with one-doctor manual operation. The two groups were compared in terms of treatment duration and curative effect. Result The treatment duration in the study was significantly shorter than that of the control group and the curative effect was significantly better than that of the control group (all P<0.05). Conclusion As for the light cured resin veneer in the treatment of patients with dentalfluorosis in the process , the four-handed technique is better than one-doctor manual operation.

The etiology of ischemic stroke in young adults is extensive, and cardiogenic embolism and cervicocephalic arterial dissection are the most common. In recent years, the proportion of atherosclerotic ischemic stroke has increased because of the increased incidence and inadequate control for the traditional vascular risk factors in young adults. However, even after a detailed and comprehensive examination, there is stil about 1/3 unknown etiology.

Objective To compare CT perfusion changes of patients 1 month after living liver transplantation with healthy volunteers. Methods Twenty perioperative patients without complications and 25 healthy volunteers underwent dynamic multi-slice CT scan. On each targeted slice, liver was divided into 3 regions of interest (ROI) according to hepatic venous drainage, i.e. anterior segment area drained by middle hepatic veins (MHV) which was most likely congested, risk area drained by both MHV and RHV which was posterior to anterior segment, reference area drained by RHV. Perfusion parameters and relative perfusion difference were calculated.Results The relative perfusion differences of anterior segment and risk area in study group were as follows: BF 44.56±43.19 vs 25.41±27.49 ［ml/(min〖DK〗·100 g)］; BV 3.91±2.45 vs 2.15±1.86(ml/100 g); PVP 41.50±39.15 vs 22.66±23.82 ［ml/(min·100 g)］. Significant differences of RPDs of BF, BV and PVP were found in anterior segment (P<0.0001) and risk area (P＜0.01) compared with control group.Conclusion In perioperative period, there are hemodynamic differences in hepatic segments drained by RHV and MHV. BF, BV, PVP of anterior segment decreases obviously.

Objective To investigate oxidative damage effect of the serum of severe preeclamptic patients on human umbilical vein endothelial cells (HUVEC).Methods (1) HUVEC were randomly divided into 4 groups according to the following:blank group as control,normal group added 20％ normal sera of pregnant women,group PE added 20％ sera of severe preeclamptic patients,and group PE + Cat added 20％ sera of severe preeclamptic patients plus 3 x 103 U/ml catalase.After cultured for 24 hours,the injury morphology and APO2.7 expression of HUVEC were detected by transmission electron microscopy and flow cytometry respectively.(2) Under the real-time scanning by laser scanning confocal microscopy,HUVEC were randomly divided into 4 groups according to the following:control group added 100 μmol/L H2O2 as positive control,normal group,group PE,and group PE + Cat.HUVEC of each group was scanned for 120 seconds to determine levels of reactive oxidative species (ROS),calcium homeostasis,and mitochondria membrane potential.Results (1) Obvious injury morphology of HUVEC was observed in group PE,and it was obviously improved by catalase in group PE + Cat.Percentage of HUVEC expressed APO2.7 was (37.8 ± 1.1) ％ in group PE,which was significantly higher than (13.4 ± 1.1) ％ in blank group or (13.5 ± 1.5) ％ in normal group,but significantly lower than (19.2 ± 1.6) ％ in group PE + Cat (all P ＜ 0.01).(2) The fluorescence intensity curves of intracellular ROS and calcium showed slowly rising in group PE,but no obvious changes in normal group and PE + Cat.The values of ROS and calcium in group PE (12.0±1.3,4.1 ±0.7) were higher than those in normal group (1.1 ±0.4,0.6 ±0.4),but lower than those in group PE + Cat (1.5 ± 0.5,0.9 ± 0.5 ; all P ＜ 0.01).Conclusion The serum of severe preeclamptic patients caused oxidative damage on HUVEC by increasing intracellular ROS generation,calcium overload,and decreasing mitochondrial membrane potential.

AIM:To investigate the effect of donor bone marrow derived dentritic cell (DC) treated with B7 - 1, B7 - 2 antisense oligonucleotide on mouse heart allografe survival time and its mechanism. METHODS: There were 7 groups of C57BL/10J (B10) mouse bone marrow DCs which were treated by 400 nM antisense oligonucleotide target to B7 -1, B7 -2 mRNA (AS B7- 1/2), B7- 1 mismatch oligo control ,B7- 2 mismatch control(mASB7- 1/2), lipofeetamine only and non-treatment, respectively. Each group of DC were named as ASB7- 1 DC, ASB7- 2 DC, mASB7 - 1 DC, mAS B7 - 2DC, and Lipo DC, respectively. RESULTS: Flow cytometer results shown that AS B7- 1/2 can inhibit B7- 1 (CD80)and B7- 2 (CD86) molecule express on DC surface, while control groups have no effects. To observe their tolerogenicity in mouse cardiac allograft model, B10→C3H heterotopic heart transplantation were performed. Recepients were received 2 x 106 of DC injection 7 days before transplantation. Results showed that both AS B7 - 1 DC and AS B7 - 2 DC can prolong mouse cardiac allograft survival time to (18.6 + 0.89) days and (23.67 + 10.73) days, respectively, compared with IL - 4 DC [ (6.22 + 0.97) days ( P < 0.01 ) ]. Two mismatch control groups can slightly prolong while oligo DC has no effect. For understanding its mechanism, each group of DC was used as stimulator to stimulated C3H spleen T cell. Results suggested that AS B7 - 1DC and AS B7 - 2 DC had less allo - stimulate function, including MLR and generation CTL and IL - 2 production than IL - 4 DC but control groups have no effect. CONCLUSION: Donor bone marrow derived DC treated with AS B7 - 1 oligo and AS B7 - 2 oligo expressed lower level of CD80 and CD86, respectively. These cells can induce allogeneic T cells anergy in vitro and markedly prolong mouse heart allograft survival time in vivo.

Objective Using the evidence-based medicine to investigate the effect of cognitive behavior intervention on physical and mental health in patients with type 2 diabetes mellitus.Methods Controlled studies related to psychological intervention on depression and glyeosylated hemoglobin in patients with type 2 diabetes were retrieved from electronic databases such as CNKI,VIP,Wanfang database and Pubmed,etc.The quality of included studies was evaluated and then data were analyzed by using RevMan5 software.Results A total of 273 articles were retrieved and finally 6 were enrolled.Results of Meta analysis were listed as follows:Cognitive behavior intervention could reduce score of Self-rated Depression Scale (SDS) in patients with diabetes mellitus.Cognitive behavior intervention could effectively reduce glycosylated hemoglobin values in patients with diabetes mellitus.Conclusions Cognitive behavior intervention can improve physical and mental health,decrease glycosylated hemoglobin and improve depression status in patients with type 2 diabetes mellitus.However,all the trials included in this review are of low quality; larger scale RCTs of higher quality are needed to confirm this conclusion.

BACKGROUND: Studies have shown that isogeneic splenocyte transplantation can induce immune tolerance and prolong liver graft survival under the effect of immunosuppressant. It is reported that bone marrow mesenchymal stem cells (BMSC) transplantation can also prolong the liver graft survival.OBJECTIVE: To observe the antirejection effect on liver grafts by simultaneous transplantation of isogeneic BMSC and spleen tissue in liver transplant rats.METHODS: Recipient Lewis rats were randomly divided into 4 groups: in acute rejection group, DA rats as the donor and Lewis rats as the recipients for liver transplantation; in cyclosporine A (CsA) group: recipients received CsA after liver transplantation. In the BMSC group, recipient Lewis rats received simultaneous BMSC transfusion and liver transplantation. In the spleen tissue group, the recipient Lewis rats received DA rat liver, spleen tissue and isogeneic BMSC transplantation. The serum cytokine levels were determined, and liver pathological changes and survival were observed postoperatively. The level of chimerism in the spleen of Lewis rats were also observed.RESULTS AND CONCLUSION: The blood levels of alanine aminotransferase, aspartate aminotransferase and total bilirubin interleukin-2 (IL-2) and interferon gamma of spleen tissue group were significantly reduced compared with the other groups (P< 0.05), but IL-6 and IL-10 were increased (P< 0.05), and donor positive cells were increased at 30 days (P< 0.05). Liver pathology revealed a mild acute rejection in liver graft of CsA and BMSC groups, severe acute rejection in acute rejection group, but no rejection in spleen tissue transplantation group. Results indicate that isogeneic BMSCs transfusion combined donor spleen tissue transplantation can induce allograft tolerance in liver transplant rats.