Objective To study the effect of follicle stimulating hormone(FSH)on the secretion of anti-mullerian hormone(AMH)in granulosa cells in patients with polycystic ovarian syndrome(PCOS).Methods From Aug.2008 to Dec.2009,33 patients with PCOS in Reproductive Medicine Research Center,Sixth Affiliated Hospital of Sun Yat-sen University were collected from 8-10 mm antral follicles and classified into the following three groups: FSH-unstimulated granulosa cells(unstimulated group,n =12),exogenous FSH-stimulated granulosa cells(exo-stimulated group,n =12)and internal FSH-stimulated granulosa cells(int-stimulated group,n =21).ELISA and real-time PCR were relatively used to measure protein and mRNA level of AMH.Luciferase reporting vector was constructed to detect the promoter activity of AMH.Results The levels of AMH secretion in PCOS granulosa cells were(11.4 ± 4.0)μg/L in unstimulated group,(7.9 ± 1.1)μg/L in exo-stimulated group and(5.6 ± 1.7)μg/L in int-stimulated group.Both the external and internal stimulation of FSH may suppress AMH secretion significantly(P ＜0.05).The mRNA level of AMH in PCOS granulosa cells were 2.5 ± 1.2 in unstimulated group,which were higher than 1.5 ± 0.5 in exo-stimulated group and 1.1 ± 0.7 in int-stimulated group significantly(P ＜0.05).The luciferase activity of AMH in PCOS granulosa cells were 11.5 ± 2.3 in unstimulated group,8.7 ± 2.4 in exo-stimulated group and 6.8 ± 2.4 in int-stimulated group.The luciferase activity of AMH in unstimulated group was significantly higher than those in exo-stimulated and int-stimulated groups(P ＜0.05).Conclusion FSH may inhibit the excessive secretion of AMH and stimulate follicle growth in PCOS granulosa cells by suppressing activity and expression of promoter.

Holter monitoring remains an useful diagnostic tool in the evaluation of syncope in children if synco-pe remains unexplained after the initial examination.But it should not be used as a screening tool.Holter monitoring is recommended in children who were suspected as cardiac syncope or who had an abnormal electrocardiogram.For chil-dren with frequent syncope,Holter monitoring is highly recommended.More information can be provided with extended Holter monitoring to 48 hours if possible.Heart rate variability is probably useful in predicting head -up tilt test diagno-sis of neurally mediated syncope.

With the globalization of higher education,the scale of foreign medical students in China has been increasing year by year.Clinical external teaching is an important connection between medical theoretical study and clinical practice.Digestive diseases are complicated and may need endoscopic therapy.Therefore,the external education for foreign students in gastroenterology has its distinctive characteristics.In recent years,in training foreign students,the gastroenterology department of our hospital has effectively improved the teaching effect by carrying out the teaching of endoscopy,establishing one plus one peer teaching,using multimedia assisted teaching of audio-visual materials,and paying more attention to improving the training teachers' English.

Surgical resection,radiotherapy and chemotherapy are the current mainstays of cancer treatments.During ovarian cystectomy,part of the normal ovarian cortex could be resected together with ovarian mass,which suppresses ovarian reserve postoperatively.This reduction in ovarian reserve is especially produced after resection of ovarian endometriomas.On the other hand,radiotherapy and chemotherapy may cause cell apoptosis,diminish blood supply in the ovaries and weaken the brake on follicular recruitment.Brought together,these mechanisms give rise to accelerated deprivation of ovarian follicles,and hence undermine fecundity of the affected individuals.Moreover,radiotherapy could result in alterations in structure and functions of uterus and other pelvic organs,which translate into increased incidence of obstetric complications later on.Currently antral follicular count and serum anti-Müllerian hormone are the most sensitive and accurate measurements of ovarian reserve.

The importance of software version in the medical device software supervision does not cause enough attention at present. First of all, the effect of software version in the medical device software supervision is discussed, and then the necessity of software version in the medical device software supervision is analyzed based on the discussion of the misunderstanding of software version. Finally the concrete suggestions on software version naming rules, software version supervision for the software in medical devices, and software version supervision scheme are proposed.
Equipment and Supplies ; Software

The mechanism of coronary artery thrombosis(CAT)caused by Kawasaki disease is not clear yet.Endothelial injury,hypercoagulability and hemodynamic change of coronary arteries are now considered as the main causes in recent studies.As The clinical symptom of acute thrombus caused by Kawasaki disease is un-conspicuous,cardiac ultrasound should be use actively and thrombolytic therapy should be performed within 12 hours after thrombus formation or the onset of acute myocardial infarction.Intervention or intravenous thrombol-ysis are both proved to be effective.Treatment with oral antitplatelet drugs,such as aspirin,combine with warfa-rin is suggested to relieve acute myocardial infarction in the chronic phase.The adverse reactions of antithrom-botic and anticoagulant drugs should be monitored though they are minor.

Objective: The FDA regulation requirements for medical device software are relatively mature and systematic, and the research on the FDA regulation thinking of medical device software helps to promote the regulation work of the medical device software in our country. Methods:Based on the comparison of the 1998 edition and 2005 edition FDA software submission guidance, the trend of the FDA regulation requirements for medical device software was analyzed, and then its regulation thinking was discussed according to other current effective software-related guidance. Results:FDA integrally improves the submission requirements for all medical device software, especially for the minor Level of Concern software, and simplifies the requirements for design specification, verification and validation, but strengthens the requirements for traceability analysis and revision history. Conclusion: The regulation work of the medical device software in our country needs to fully consider the particularity of the medical device software, strengthen the audit of software quality management system, implement the software traceability analysis, and determine the regulation requirements for software change.

Multiglycosidorum Triptergii are widely used in China. We treated 37potients who had suffered from child asthma for 12 weeks with this drug (1.0～1.5mg/kgday) and observed for about 8 months. The results showed that 33 of them were curedor relieved. The effective rate was 89.18%. In order to study its immune mechanism, the percentage of T subsets and T supperessive rate were measured for 27 patients.Theresults showed that CD3~3 and CD4~4 subpopulation had no change,but the percentage ofCD8~+ increased from 25.36?5.16% to 28.96?3.03%,the ratio of CD4~+/CD8~+ declined(P

Objective Explore the effect lactobacillus on allergic rhinitis (AR) rat serum Il-4 ,Il-5 ,IFN-γ concentration and the influence of immune markers .Methods SD rat 60 were randomly divided into 4 groups ,each group of 10 only .Lactic acid bacteria group every day with lactobacillus living bacterium preparations irrigation stomach 3 weeks ,establish rats LuanBai protein sensitiza-tion model ,positive drug control group irrigation stomach loratadine ;Another 20 rats used for passive cutaneous anaphylaxis (PCA) ,inspection in the rat model preparation and treatment .Determination test group rats serum IL-4 ,IL-5 ,IFN-γ,the content of Eos in nasal secretion was detection .Results Model group IL-4 ,IL-5 content was significantly higher than normal control group (P<0 .05) ,IFN-γlevels were low ,lower than the normal control group(P<0 .05) .Lactic acid bacteria group IL-4 ,IL-5 content were significantly lower than the model group(P<0 .05) ,IFN-γcontent increased ,higher than the model group(P<0 .05) .The in-dex of lactobacillus group with positive drug compared difference was statistically significant .Conclusion Lactic acid bacteria can cut AR rats serum IL-4 ,IL-5 level ,increase IFN-γlevel ,adjust Th1/Th2 lymphocyte subsets balance ,reduce nasal mucosa allergic inflammation .

Objective To study the relationship between X-ray features and pathologic infiltration of early gastric cancer.Methods 19 cases of early gastric cancer were analyzed, which were proved by pneumobarium double-contrast examination, operation and pathology. Results There were 9 cases of mucosa cancer and 10 cases of submucosa cancer in 19 patients of early gastric cancer certified by operation. The main X-ray features of mucosa cancer were: a lessened flexibility of local wall, indistinct outline, small trivialities became small nodes and small ditches became indistinct, they looked like pomegranate seeds. The main X-ray features of submucosa cancer were: the local wall was stiff, the density was high, there were small filling defects and small irregular niches, the rugae in the zone involved by the tumour became stiff, large even disappear, small trivialities and small ditches were destroyed. The X-ray findings of early gastric cancer had functional changes, abnormal contraction, more liquid in stomach.Conclusion Pneumobarium double-contrast examination is a voluable method for the diagnosis of early gastric cancer.