Objective To evaluate the clinical value of fast detection of high sensitivity C-reaction protein(hs-CRP)in distinguis-hing between transudate and exudate.Methods 100 cases of patients were enrolled in the investigation.The levels of TP,LDH, GLU in serum and pleural effusion respectively,as well as the level of hs-CRP in the pleural effusion.Results According to Light standard,82 cases of patients were diagnosed as transudate(transudate group)and 18 cases of patients were diagnosed as exudates (exudates group).The sensitivities of Rivalta test,TP(pleural effusion)/TP(serum)and LDH(pleural effusion)/LDH(serum) were 83.14%,80.49% and 79.27%,respectively.Hs-CRP showed 84.15% sensitivity and 27.78% specificity at a cutoff value of 5 mg/L.TP(pleural effusion)/TP(serum)and LDH(pleural effusion)/LDH(serum)had the highest specificities,and both were 100.00%.The levels of LDH(pleural effusion)/LDH(serum)and hs-CRP in transudate group were significantly higher than those in exu-dates group(P<0.05).Combined detection of hs-CRP,TP(pleural effusion)/TP(serum)and LDH(pleural effusion)/LDH(serum)had the highest specificity (100.00%).Conclusion The fast detection of hs-CRP in pleural effusion is sensitive,combining with other tests can prove its specificity.The fast assay of hs-CRP has special clinic value in the distinguish between exudate and transudate.

Objective To study the neuroprotective effects of citicoline(CC) on the toxicity induced by 6-OHDA towards dopaminergic mesencephalic neurons in primary culture-Parkinson′s disease(PD) model in vitro and its mechanism. Methods Mesencephalic neurons in culture were prepared from embryonic 15-day Wistar rats.Cultures were treated for 6,8,10 d with various concentrations of CC(2,1,0.1,0.01 and 0.001 mmol?L~(-1)).At 11th day,the cultures were co-treated with the toxin 6-OHDA(50 ?mol?L~(-1)) for 0.5 h,the cells were collected.Seven groups were categorized as follows:CC(2,1, 0.1,0.01 and 0.001 mmol?L~(-1)) +6-OHDA,control and(6-OHDA) group.The cell viability was evaluated with MTT assay.Intracellular free Ca~(2+),[Ca~(2+)]i,was labeled by using the fluorescent dye Fluo3-AM and detected by flow cytometer.By measuring the intracellular Rhodamine(123 fluorescence) density with flow cytometer,mitochondrial membrane potential(MMP) was evaluated.(Results Cultures) were treated with 2,1 and 0.1 mmol?L~(-1) CC,the viability of cell was increased(P

Objective Explore the effect lactobacillus on allergic rhinitis (AR) rat serum Il-4 ,Il-5 ,IFN-γ concentration and the influence of immune markers .Methods SD rat 60 were randomly divided into 4 groups ,each group of 10 only .Lactic acid bacteria group every day with lactobacillus living bacterium preparations irrigation stomach 3 weeks ,establish rats LuanBai protein sensitiza-tion model ,positive drug control group irrigation stomach loratadine ;Another 20 rats used for passive cutaneous anaphylaxis (PCA) ,inspection in the rat model preparation and treatment .Determination test group rats serum IL-4 ,IL-5 ,IFN-γ,the content of Eos in nasal secretion was detection .Results Model group IL-4 ,IL-5 content was significantly higher than normal control group (P<0 .05) ,IFN-γlevels were low ,lower than the normal control group(P<0 .05) .Lactic acid bacteria group IL-4 ,IL-5 content were significantly lower than the model group(P<0 .05) ,IFN-γcontent increased ,higher than the model group(P<0 .05) .The in-dex of lactobacillus group with positive drug compared difference was statistically significant .Conclusion Lactic acid bacteria can cut AR rats serum IL-4 ,IL-5 level ,increase IFN-γlevel ,adjust Th1/Th2 lymphocyte subsets balance ,reduce nasal mucosa allergic inflammation .

Based on the ICF framework and functional vision theories, some elements of an evaluation system for visually impaired children were extracted, including body structure and function, activities and participation, and environmental factors.

Castleman Disease ; complications ; Dendritic Cell Sarcoma, Follicular ; complications ; Humans

Objective To evaluate the clinical value of combined detection of serum CEA, CA125,CA153 and CA199 in lung cancer diagnostec. Methods The serum level of CEA,CA125,CA153 and CA199 in 58 patients with lung cancer,38 patients with benign lung diseases and 40 healthy persons were determinated by Chemiluminesent immunoassay. The data were analyzed to evaluate the diagnostic value of the combination use of four markers. Results The serum levels of CEA,CA125,CA153 and CA199 in lung cancer group were 5.6 (1.0-619. 0) μg/L,124. 5 (5.3 - 994. 8) U/ml,14.4(3. 1 - 800. 0) U/ml and 21.8(2.0 - 1200.0) U/ml respectively ;The serum levels of CEA, CA125, CA153 and CA199 in benign lung diseases were 1.9 (0. 6 -14.4) μg/L, 17. 7 ( 1.8 - 303.6 ) U/ml, 6. 8 ( 2. 4 - 20. 1 ) U/ml and 9. 3 ( 2. 0 - 82. 6 ) U/ml respectively. The serum levels of CEA, CA125, CA153 and CA 199 in healthy controls were 1.4 (0. 6 - 5.0 ) μg/L, 9.6 (4. 4 -36. 5 ) U/ml,6. 6 (3.8 - 19. 9) U/ml and 6. 4 (2.0 - 58. 1 ) U/ml respectively. The levels of markers in the lung cancer group were significantly higher than those in patients with benign lung disease and healthy controllers (Ps ＜ 0. 01 ). The sensitivity of combined measurement was 94. 8%, significantly higher than a single measurement ( CEA 48. 3% ,CA125 75.9% ,CA153 20.7% ,CA199 31.0% ,Ps ＜0. 01 ). Conclusion The combined detection of CEA, CA125, CA153 and CA199 can significantly improve detection sensitivity of lung cancer and might provide valuable laboratory proof for early diagnosis of lung cancer.

Objective To evaluate the advantage of uterine appendages lump operation by laparoscopy and the choose of the patient. Methods 215 cases of ovrarian tumor operation and fallpian tubes operation by laparoscopy,50 cases of similar period and similar operation indication were chosen by laparoscopy or by abdorminial operation apiece, blood loss, postoperative analgesic rate, the time of using antibiotics, hospitalization day were compared. Results Uterine appendages lump operation by laparoscopy were all operated successfully, compared with abdorminial operation, the laparoscopy group had less blood loss,less drug after operation,less pain, shortened hospitalization day.Conclusion As long as choosing the appropriate cases before the operation, uterine appendages lump operation by laparoseopy has the advantage of minimal trauma, recovery fastly,less postoperative complication and safety.

Objective To explore the role of serum levels of Klotho protein and fibroblast growth factor(FGF)-23 in patients with chronic kidney disease(CKD).Methods A total of 160 patients with CKD were recruited into CKD group in this study,160 healthy controls were selected in the control group from March 2014 to March 2016.Basic clinical data,blood biochemical index,serum level of Klotho protein and FGF-23 were analyzed and compared between the two groups.Results The hemoglobin,serum albumin,blood calcium,glomerular filtration rate,Klotho protein levels in the CKD group were lower than those in the control group,and the serum creatinine,blood urea,blood serum phosphorus and FGF-23 were higher than those in the control group(P<0.05).With the progress of CKD stages,hemoglobin,glomerular filtration rate,Klotho protein levels gradually decreased,serum creatinine,blood urea,blood serum phosphorus,FGF-23 level increased(P<0.05).The levels of FGF-23 were negatively related to hemoglobin,glomerular filtration rate,Klotho(r=-0.584,0.692,-0.724)and were positively correlated to serum creatinine,blood urea,blood serum phosphorus(r=0.814,0.703,0.527).The levels of Klotho protein were positively related to hemoglobin,glomerular filtration rate(r=0.612,0.685),and were negatively correlated to serum creatinine,blood serum phosphorus,blood urea,FGF-23(r=-0.720,-0.690,-0.519,0.724).Conclusion High concentrations of FGF-23 and Klotho protein with low concentration were not only related to calcium phosphate metabolic disorders of patients with CKD,and were also associated with the prognosis of patients with CKD,which might be early biomarkers and predictor in patients with CKD.

Objective To share the data of Fully Automatic Biochemistry Analyzer SINNOWA -B300 with Hospital Information System (HIS). Methods The main characters and functions of the Data Pipeline of PowerBuilder were analyzed,and designed application program to connect Access Database of analyzer and Oracle Database of HIS. Results The analyzer can input automatically into Oracle Database of HIS. Conclusion The use of PowerBuilder data pipeline object combined with programmer achieves real-time data sharing,the simple and reliable technology can provide a technical reference for the development of LIS of hospitals.

0.05).Patients with mucitis of the study group and the control group were 0 and 7.The difference in statistics analysis was significant(P