Objective To understand the prevalence of major non-communicable chronic diseases among the adults in the airport district of Beijing.Methods With the same proportion and multi-stage stratified cluster sampling,the prevalent rates of primary existing chronic diseases were investigated in 650 residents aged over 18 years and lived in Beijing for more than 6 months.Each participant was invited to receive a set of standardized questionnaire,physical examinations and laboratory tests.Results The prevalent rates of hypertension,diabetes,dyslipidemia,acute myocardial infarction and the stroke were 26.0%,5.3%,39.9%,1.3% and 2.0%,respectively.The awareness rates for hypertension and diabetes were 54.4% and 3.6%,respectively.Conclusion The prevalent rates of chronic non-communicable diseases of the adults in this area were in higher levels.The relevant department should pay more attention to it.

BACKGROUND:In recent years, research on tendinopathy is becoming a hot topic in the sports medicine and rehabilitation, and the extracelular matrix plays a considerable role in the occurrence, development and healing of tendinopathy.
OBJECTIVE: To explore the relationship between tendinopathy and the extracelular matrix.
METHODS: CNKI, VIP, CBM, Wanfang, PubMed and Embase were retrieved for articles related to colagen, protein, glycoprotein and matrix remodeling published since 2000.
RESULTS AND CONCLUSION:After tendinopathy, there are obvious changes in colagen, proteoglycans, glycoprotein and related enzymes; and these changes also affect the reconstruction and repair of tendinopathy. Currently, most of researches focus on the tendon rather than the bone-tendon junction. This means that there are no in-depth studies on the correlation between many important proteins and tendinopathy, and the effects of exercise intervention.

Objective To study the chemical constituents from EtOAc extracts of Paeonia lactiflora. Methods Compounds were isolated by various chromatographic techniques and structures were elucidated on the basis of spectral analysis. Results Seventeen compounds were obtained and their structures were identified as l,2,6-benzenetriol-l-O-α-D-glucoside (1), paeoniflorin (2), 4-methylpaeoniflorin (3), albiflorin (4), paeonidanin (5), benzoylpaeoniflorin (6), 4-methylbenzoylpaeoniflorin (7), benzoylalbiflorin (8), paeonidanin A (9), galloylalbiflorin (10), debenzoylalbiflorin (11), 4',5-dihydroxyflavanone-7-O-β-D-glucoside (12), 5,7-dihydroxy flavanone-4'-O-β-D-glucoside (13), (+)-catechin (14), gallic acid (15), vanillic acid (16), and 1,2,3-benzenetriol (17). Conclusion Compound 1 is a new compound named paeoniphenoside. Compounds 12 and 13 are firstly obtained from genus Paeonia L., and compounds S and 9 are isolated from P. lactiflora for the first time.

T was genotyped by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP)in all the patients.Results The occurrence of CR in this population was 23.3%(70/300).There was CYP3A4 894C/T polymorphism in the study population.The frequencies of the three kinds of genotypes(CC,CT,TT)in CR group and non-CR(NCR)group were 45.7%,50.0%,4.3% and 63.5%,31.7%,4.8%,respectively.The frequency of TT genotype was significantly higher in NCR group than that in CR group(OR=2.06,95% CI:1.201-3.547,P=0.020).C allele carriers were more likely to develop clopidogrel resistance compared with that of T allele carriers(OR=1.59,95% CI:1.037-2.442,P=0.023).Conclusion CYP3A4 gene 894C/T polymorphism is associated with the risk of CR,and C allele carriers may be a possible genetic susceptibility factor for patients with CR.

Objective To investigate the clinical value of echocardiography combined with genetic testing in the fetal cardiac rhabdomyoma. Methods Thirty-three fetal cardiac rhabdomyoma cases diagnosed by fetal echocardiogram in Beijing Anzhen Hospital from Jan. 2011 to Oct. 2015 were enrolled in a retrospective analysis. The results of other examination and pregnancy outcomes of them were followed up, the genetic characteristics of cardiac rhabdomyoma were summarized on the basis of pathology and genetics examination results. Results The pregnancy outcomes:24 cases were terminated pregnancy, 4 cases were born and 5 cases were lost. The results of ultrasound, pathology and genetic examination were detailed in 8 cases. Pathological examination: the typical characteristics of cardiac rhabdomyoma were found in the 8 cases with cardiac rhabdomyoma. The tumor tissue was composed of irregular and swelling shape of cardiomyocytes, and the cytoplasm was vacuole like, which was characteristic of“spider like cells”through microscopic observation. The geneticdetection results: 7 cases had tuberous sclerosis complex (TSC) gene mutation, TSC gene abnormalities were not detected in 1 case. Among the 7 cases with TSC gene mutations, 6 cases were with TSC2 gene mutation and the other 1 case was with TSC1 gene mutation. The family gene was investigated in the 5 cases, which including 3 cases of TSC gene mutation in mother passed on to the fetus (1 case with family of three generations of genetic) and 2 cases of spontaneous TSC gene mutation in the fetus. Conclusions Prenatal echocardiography combined with genetic detection have important clinical significance, which not only can clear if cardiac rhabdomyomas were associated with TSC, but also can clear the TSC gene mutation source. So as to further guide the perinatal management.

ObjectiveTo investigate the distribution of types of human papillomavirus(HPV)in cervical cancer Xinjiang Uyghur women.MethodsFrom june 2008 to April 2010,patients in the gynecological departmen of people's Hospital of Xinjiang Uygur Autonomous Region,who was gidiagnosed cervical cancer and came from southern of Xinjiang.Cervical cells of these patients were collected,HPV genotyping DNA chip was used to detect HP-DNA of each collected sample.ResultsTotally 120 wommen were screened,the infecton rationof HPV was 95.8%(115/120).The positive rate of HPV-16 infection was 69.6%(80/115);other highrisk HPV infection rates were ranked from high to low as HPV-56,HPV-33,HPV-18,HPV-45,HPV-58,HPV-51,HPV-59,HPV-31,HPV-52,HPV-39,HPV-68,HPV-73,low-risk HPV infection rates were ranked from high to low as HPV-11,HPV-42,HPV-43,HPV-6.However,HPV-35,HPV-53,HPV-66,HPV83,HPV-MM4 and HPV-44 were not detected.Single infection of HPV16 or HPV56 was 53.9%(62/115)and 17.4(20/115)respectively,ultiple infection rate was 29.6%(34/115).ConclusionHPV-16 is the most common types in cervical cancer of Xinjiang Uyghur women.HPV56 maybe susceptible to the Xinjiang Uyghur women,reflecting the specificity of HPV infection in cervical cancer of Xinjiang Uygur women.It needs deep study to development a suitable HPV vaccines.

Using a micro-column packed with polyethylenimine aminated glycidyl methacrylatE-grafted-polytetrafluoroethylene fiber made by ourselves as the adsorption material, the adsorption behaviors of Pb and Cd under different conditions were studied by inductively coupled plasma optical emission spectrometry. The conditions for the preconcentration of Pb and Cd such as pH, flow-rate, time and eluent acidity were optimized. The results show that the PTFE-g-GMA-PEI Fiber has excellent adsorption property and high adsorption capacity for Pb2+ and Cd2+. The Enrichment factors for Pb2+ and Cd2+ were 30 and 80, respectively and the detection limits were 3.5 μg/L and 0.15 μg/L, respectively, the relative standard derivations were 1.5% and 0.6%(n=9)respectively. When the single ion concentration was 50 μg/L, the sampling frequency was 9 and 18 times/h, respectively. The method was applied to the simultaneous determination of trace lead and cadmium of several Chinese traditional medicine samples, the standard recovery of samples was 90%-108%.

Objective To evaluate the effect of delayed preconditioning with morphine on ischemic cerebral injury in mice and the role of classical protein kinase C (cPKC).Methods Forty male BALB/C mice,weighing 20-22 g,were randomly divided into 4 groups (n =10 each):sham operation group (group S),ischemic cerebral injury group (group ICI),morphine preconditioning group (group MP) and cPKC inhibitor Go6983 group (group G).Ischemia was induced by middle cerebral artery occlusion (MCAO).In S group,the middle cerebralartery was only exposed but not occluded.In MP group,morphine 10 mg/kg was injected intraperitoneally 24 h before MCAO.In G group,morphine 10 mg/kg was injected intraperitoneally 24 h before MCAO and 5 μl Go6983 (6nmol) was injected into the left lateral cerebral ventricle immediately before MCAO.The neurologic deficit was evaluated and scored according to neurological disability status scale in a blind nanner 6 h after MCAO.The animals were sacrificed and brains were immediately removed for measurement of the brain edema and infarct volume.Apoptotic rate was calculated.Results Compared with S group,the neurologic deficit scores,infarct volume,brain edema and apoptotic rate were significantly increased in ICI,MP and G groups (P ＜ 0.01).Compared with group ICI,the neurologic deficit scores,infarct volume,brain edema and apoptotic rate were significantly decreased in group MP (P ＜ 0.01),and no significant change was found in the parameters mentioned above in group G (P ＞ 0.05).Conclusion Delayed preconditioning with morphine can reduce ischemic cerebral injury in mice and activation of classical cPKC signaling pathway is involved in the mechanism.

Background and purpose:Studies have shown that DOC-2 could work as a potential tumor suppressor geue,and the role of DOC-2 in terms of the inhibition of cell growth and its mechanism remain unknown.Our paper is to investigate the effect and mechanism of DOC-2 expression on the tumorigenesis viability of ovarian cancer cell line HO-8910 from the aspects of clone efficiency,cell cycle and animal model test.Methods:Three cell lines were used including HO-8910,8910-P93(transfected with DOC-2 gene) and 8910-pcDNA3.1(transfected with the vector pcDNA3.1).Firstly,soft agar method was used to measure the clone efficiency.The cell cycle were analyzed by flow cytometer.The tumorigenesis viability was compared by athymic mouse test.Results:After being transfected with DOC-2 gene,the clone efficiency of 8910-P93 was markedly reduced.There was no difference between the 8910-pcDNA3.1 and HO-8910.G1 and G2 arrest were observed for 8910-P93.The athymic mouse test showed that the neoplasm derived from 8910-P93 was much smaller than that in the controls.Conclusions:DOC-2 could iniibit the tumorigenesis viability of human ovarian cancer line HO-8910.