Objective To evaluate the efficacy and safety of ultrasound-guided percutaneous umbilical blood sampling. Methods Eight hundred and seventy-two pregnancies with proper indications underwent ultrasound-guided percutaneous umbilical sampling. The data of each procedure were prospectively collected and subsequently analyzed for successful rate and pregnancy outcomes. Results Of 872 ultrasound-guided percutaneous umbilical sampling, 62.7% were done successfully at the first attempt, 36.0% were done successfully by two to four attempts. 31.4% fetuses had mild procedure-related complications and 0.3% fetuses had severe complications. Conclusion We should improve the efficacy and safety of ultrasound-guided percutaneous umbilical sampling and make it a correct, safe and effective procedure for prenatal diagnosis.

BACKGROUND:Orthodontic treatment is a mechanical force for tooth to cause the remodeling of periodontal tissue, produced by the tooth movement. The main aspect of orthodontic periodontal tissue remodeling is the alveolar bone. Insulin-like growth factor is an important factor in the remodeling of periodontal tissue, which plays an important role in the growth, differentiation and growth of the cels. OBJECTIVE:To review the role of insulin-like growth factors in periodontal tissue remodeling. METHODS: A computer-based retrieval of PubMed, CNKI and Guizhou Province Digital Library Database was performed to search articles related to the role of insulin-like growth factors in periodontal tissue remodeling. The keywords were “insulin-like growth factor; periodontal tissue; remodeling” in English and Chinese, respectively. RESULTS AND CONCLUSION: Insulin like growth factor belongs to the insulin family, a kind of peptides, which can promote the migration, proliferation, differentiation, colagen and matrix synthesis and alkaline phosphatase activity of osteoblasts, fibroblasts and mesenchymal cels in the periodontal ligament. It also plays an important role in the repair of injury. During orthodontic treatment, the use of suitable orthodontic force combined with insulin-like growth factor can promote periodontal tissue remodeling, accelerate the orthodontic tooth movement and shorten the treatment time for patients.

Objective:To understand general hospital medical staff status quo and characteristics of blood -borne occupational exposure , analysis its ethics factor , to make the prevention countermeasures of blood -borne oc-cupational exposure to provide scientific basis .Methods:From January 2013 to December floor all the medical staff of blood -borne occupational exposure cases were retrospectively analyzed .Results:A total of 101 medical staff blood-borne occupational exposure , which is given priority to with the nurse , accounted for 60 .40%;More con-centrated in under 30 employees, accounted for 70.29%;Occupational exposure personnel distribution in the ma-jority with surgical department (42.57%), followed by the physician (36.63%);Wards (60.40%), the operat-ing room (15.84%) and therapy (11.88%) are the sites of occupational exposure often happen;Sharp injury in the composition of the ratio of 87.12%, in the first place, in which a proportion (23.76%), pull out the needle (18.81%) and blood (15.84%), surgical suture (14.85%) and transfusion injection (13.86%) as the sharp injury of frequent occurrence of occupational exposure;Exposure is given priority to with hepatitis b ( 61 .38%) . Conclusion:Medical staff blood-borne occupational exposure risk is high , the hospital infection control personnel must pay attention to occupational exposure of the whole education , strengthen the administration of the occupation-al exposure of ethics , reduce the risk of occupational exposure and injury .

BACKGROUND:Orthodontic tooth movement is based on the periodontal tissue remodeling. In the exogenous factors accelerating orthodontic tooth movement, Chinese herbal medicine has become a research hotspot because of its wide resources, low cost, easy to extract, mild effect, smal toxic, less side effects and drug resistance. OBJECTIVE: To summarize the role of Chinese herbal medicine in the periodontal tissue remodeling during orthodontic tooth movement. METHODS:A computer-based retrieval of CNKI, Wanfang and PubMed databases was performed for articles related to Chinese herbal medicine for improving orthodontic tooth movement published before 2014. The keywords were “Chinese herbal medicine, orthodontic tooth movement, periodontal tissue remodeling” in Chinese and English, respectively. RESULTS AND CONCLUSION:Erigeron breviscapus, Salvia, teasel, Drynaria, baicalin, evening primrose oil as Chinese herbs are most widely used in the promotion of periodontal tissue remodeling, characterized as wide resources, low cost, easy to extract, mild effect, low toxicity, less drug resistance. In the clinical orthodontic treatment, it is hoped to accelerate orthodontic tooth movement and shorten the treatment time. Therefore, under the appropriate corrective force, Chinese herbs can be used properly to improve periodontal tissue repair and remodeling, which can improve the microcirculation of periodontal tissue, increase the local blood flow, promote bone formation and repress bone resorption.

Endemic dental fluorosis has been reported in some regions of the world. China seemed to have high prevalence of endemic dental fluorosis, especially in southwest China. It is now most likely that excessive fluoride intake during enamel development play a key role in the pathogenesis of dental fluorosis. However, excessive intake of fluoride-induced cellular and molecular mechanisms of dental fluorosis are not entirely conclusive. Scholars at home and abroad have made a lot of research on pathogenesis of enamel fluorosis by using various experimental techniques. More recent studies mainly suggest that endoplasmic reticulum stress and calcium overload-associated apoptotic pathway may participate in fluoride excess-evoked pathogenesis of dental fluorosis. Furthermore, the functional changes of enamel matrix protein and protease activity may be involved in the pathological event. This paper summarized the recent research progress on this topic.

Objective To observe the change of serum ischemia modified albumin (IMA) in continuous ambulatory peritoneal dialysis (CAPD) patients.Methods Seventy-four end stage renal disease patients undergoing CAPD more than 3 months (CAPD group) were divided into 2 groups according to clinical symptoms of cardiovascular diseases:CAPD symptoms group (29 cases) and CAPD asymptomatic group (45 cases).Seventy healthy subjects were selected as control group.The serum IMA level and abnormal rate of electrocardiogram and heart color ultrasonic were examined and compared.Results The serum IMA level in CAPD group was significantly higher than that in control group [(92.33 ± 17.17) kU/L vs.(69.63 ± 9.24)kU/L,P＜ 0.01].The serum IMA level in CAPD symptoms group was significantly higher than that in CAPD asymptomatic group [(109.37 ± 21.34) kU/L vs.(85.31 ± 8.58) kU/L,P ＜ 0.05].The elevatory rate of serum IMA level and abnormal rate of electrocardiogram and heart color ultrasonic in CAPD symptoms group were significantly higher than those in C APD asymptomatic group [37.9％ (11/29) vs.15.6％(7/45),62.1％ (18/29) vs.22.2％ (10/45),P ＜0.05].Conclusions The serum IMA level in CAPD patients is elevatory.Serum IMA level is significantly higher in CAPD patients with cardiovascular disease clinical symptoms,it can be used for diagnosis of myocardial damage in CAPD patients.

Although the mechanism by which migratory trophoblasts reach the spiral arteries is currently obscure, yet the process has been noted to involve the attachment, adhesion and migration of trophoblasts on the blood vessel walls. To test this, micropipette and flow chamber were used to measure quantitatively the adhesion forces and migration of early gestation human trophoblast cells (TCs) cultured on the glass slides coated with type I rat collagen or cultured with human umbilical vein endothelial cells (HUVECs). The results showed that the interdiction of integrin beta1 interaction remarkably reduced the adhesion forces of TCs to type I rat collagen or endothelial cells, and remarkably resisted the displacement of TCs induced by shear stress. By contact between TCs and endothelial cells, the TCs' adhesion force and TCs' resistance to shear stress were significantly enhanced. The results indicated that the contacts of TCs with endothelial cells enhanced the adhesion forces of human TCs, and regulated the migration of human TCs by shear stress.
Adult ; Cell Adhesion ; drug effects ; Cell Movement ; drug effects ; Cells, Cultured ; Coculture Techniques ; Female ; Human Umbilical Vein Endothelial Cells ; cytology ; Humans ; Integrin beta1 ; physiology ; Trophoblasts ; cytology

OBJECTIVETo investigate the synergistic effect of transforming growth factor-beta1 (TGF-beta1) and platelet-derived growth factor-BB (PDGF-BB) on the expression of integrin beta3, in periodontal membrane of rat orthodontic tooth.

METHODSAn orthodontic tooth movement model was established. Up to 32 experimental rats were randomly divided into four groups according to a random number table. The four groups were injected with 1% PBS, TGF-beta1 (5 ng), PDGF-BB (10 ng), and combined TGF-beta1 (5 ng) and PDGF-BB (10 ng) in the buccal submucosal, respectively. The volume injected in each group was 0.1 mL. The animals were then sacrificed on the 10th day. The left maxillary first molar and periodontal tissue were taken. Different expressions of integrin beta3 were detected in periodontal tissues through immunohistochemistry. Mean optical density (OD) values of the positive fields were examined. The data obtained were analyzed through ANOVA. The data followed normal distribution, and were compared via t-test.

RESULTSCompared with the control groups, the expression of integrin beta3 was higher in the experimental groupin tension sides (P < 0.01). Significant differences in tension sides between the single-injection groups and the combined group were observed (P < 0.01). Compared with the control groups, the expression of integrin beta3 was higher in the experimental group in compression sides (P < 0.05). In addition, there was no significant differences in compression sides between the single-injection groups and the combined group (P > 0.05).

CONCLUSIONIn terms of local regulatory factors, TGF-beta1 combined with PDGF-BB enhance the expression of integrin beta3 in the periodontal membrane and accelerate periodontal remodeling. The synergistic effect of the two growth factors is better than the single growth factor.

Objective To investigate cognitive status and influencing factors for hand hygiene(HH)among health care workers(HCWs),and provide basis for scientific management of HH.Methods In April 2013,HCWs in a general hospital were selected by randomly sampling method,questionnaires were used to survey the implementation of HH in recent one month and HCWs’cognition on knowledge about HH.Results A total of 750 HCWs were in-vestigated,652 available questionnaires were collected.The frequency of hand washing and hand disinfection per day among most HCWs were 10 - 19 times,accounting for 46.62% and 47.85% respectively;30.52% of HCWs washed their hands for ≥30 seconds each time,60.58% of HCWs dried hands with paper towel after washing hands,57.21 % of HCWs abided by six-step hand washing method.The overall correct rate of cognition on ten op-portunities that requiring HH in clinical practice was 68.68%.The main factors influencing the implementation of HH were as follows:skin irritation of hand sanitizer and hand disinfectant subjectively considered by HCWs (63.34%),inadequate hand washing facilities(41 .10%);high cost of hand sanitizer ,hand disinfectant,and dry paper towel (38.96%),et al.Conclusion In addition to intensifying education on HH,installing rational HH facili-ties and improving HH standard are key points in strengthening HH in general hospital.

Objective:To survey the expression of MMP-1,MMP-2 of human periodontal ligament cells(HPDLCs)treated by tea polyphenols(TP)and lipopolysaccharide(LPS).Methods:HPDLCs were in vitro cultured in vitro and treated by TP(200 μg/ml) and /or LPS(100 μg/ml)for 24,48 and 72 h respectively,the secretion of MMP-1 and MMP-2 were examined by ELISA,MMP-1 and MMP-2 mRNA expression was examined by real-time PCR.Results:The secression and mRNA expression of MMP-1 and MMP-2 of HPDLCs increased by LPS treatment and significantly inhibited by TP at the different times.Conclusion:TP can inhibit the col-lagen degradation of HPDLCs mediated by LPS.