Objective: To study the chemical constituents in the roots of Aconitum bulleyanum Diels. Methods: The air-dried roots of A. bulleyanum were powdered and extracted by methanol with percolation. After removing the solvent under reduced pressure, the crude extract was dissolved in 1. 5％ HCl solution, and then basified to pH 9 by NaOH (5％) and extracted by ethyl acetate to ob-tain crude alkaloidal extract after ethyl acetate removal. The alkaloidal extract was isolated and purified by column chromatography, and their structures were identified based on spectral analysis ( 1 H-NMR, 13 C-NMR, MS) . Results:Totally 12 diterpenoid alkaloids were isolated from A. bulleyanum and characterized as foresaconitine (1), crassicauline A (2), chasmanine (3), talatisamine (4), 14-debenzoyl-franchetine (5), pengshenine A (6), crassicautine (7), yunaconitine (8), franchetine (9), liljestrandisine (10), transconitine B (11) and pseudoaconine (12). Conclusion:Compounds 3-7, 10-12 are isolated from this plant for the first time.

Fatty Liver ; Humans

Objective To study the effect of rosuvastatin(RSVT) on the vascular endothelial function(VEF) ,inflammatory fac‐tors(IF) and prognosis in patients with acute coronary syndrome (ACS) after percutaneous coronary intervention(PCI) .Methods Eighty cases of ACS patients with PCI in our hospital selected from July 2010 to July 2013 were randomly divided into observed group and control group ,40 cases in each group .The observation group were given interference treatment with RSVT while the control group received conventional treatment .The VEF ,IF and prognosis were compared between groups .Results The VWF at 4 weeks after PCI in observation group was lower than that in control group [(92 .6 ± 12 .3)% vs .(105 .4 ± 13 .6)% ,P<0 .05];The ET‐1 at 4 weeks after PCI in observation group was lower than that in control group[(55 .6 ± 5 .6)ng/L vs .(67 .8 ± 7 .4)ng/L ,P<0 .05] .The NO at 4 weeks after PCI in observation group was higher than that of control group[(78 .6 ± 9 .4)μmol/L vs .(63 .2 ± 9 .5)μmol/L ,P<0 .05] .The CRP at 4 weeks after PCI in observation group was lower than that in control group[(5 .4 ± 2 .2) mg/L vs .(10 .5 ± 4 .1)mg/L ,P<0 .05] .The incidence of CVE was 5 .00% (2/40) ,restenosis rate was 2 .50% (1/40) in observa‐tion group ,which was significantly lower than those of the control group [30 .00% (12/40) ,10 .00% (4/40)] ,the differences were statistically significant(P<0 .05) .Conclusion RSVT could effectively improve the VEF and reduce inflammation ,CVE and rest‐enosis rate in patients with ACS after PCI .

Objective: To construct a high effective eukaryotic expre ss ing vector containing mcpr1 gene. Methods: mcpr1 gene w as amplified by PCR from the plasmid T-easy/ mcpr1, then PCR product was in serted into eukaryotic expressing vector pcDNA3.1/V5-His B. The positive recomb inant was identified by PCR analysis, HindIII and BamHI restriction analysis and Sequence analysis. Results: A 400 bp DNA fragment was amplified from the recombinant. Sequence analysis and restriction digest demonstrated tha t the mcpr1 gene was successfully inserted into pcDNA3.1/V5-His B plasmid. Conclusion: The eukaryotic expressed vector pcDNA3.1/V5-His B/ mcpr1 has been successfully reconstructed.

Objective To observe the effect of Jiangzhi Yigan Chongji (JYC) on the nonalcoholic fatty liver tissue PPAR? and Trx mRNA expression,and explore the mechanism of treating fatty liver. Methods The model was made by feeding high-fat diet and the rats were divide into 3 groups:normal group,model group and treated group. Result Expression of liver tissue PPAR? and Trx mRNA in the model group were both decreased. JYC can increase their expression of liver tissue of model rats. Conclusion It is likely to be one of the important mechanisms for JYC in treating nonalcoholic fatty liver.

Amyloidosis ; Gastrointestinal Hemorrhage ; Humans ; Immunoglobulin Light-chain Amyloidosis

AIM: To investigate the role of Smad7 in the Smad2 expression induced by transforming growth factor-?_1(TGF-?_1) in rat peritoneal mesothelial cells(PMCs).METHODS: Rat PMCs were cultured at different doses of TGF-?_1 (0,1.25,2.5,10 ?g/L) for different time(0,5,15,30,60,120 min).PCDNA3-Smad7 was then transfected into cultured rat PMCs by lipofectamine,and the cells were stimulated like the above.Endogenous Smad2 and Smad7 expression was evaluated by RT-PCR and Western blotting.RESULTS: TGF-?_1 induced increase in Smad2 mRNA and protein expression at 5 min,peaked at 30 min,and declined to baseline levels at 120 min, which was in a time-dependent manner.TGF-?_1 also induced Smad7 mRNA expression at 5 min,and then declined,down to the lowest at 30 min,but at 60 min it increased again.Smad2,Smad7 mRNA and protein expression induced by TGF-?_1 were also dose-dependent.After transfection,overexpressions of Smad7 mRNA and protein in rat PMCs were observed,which did not decline with time.The expression of Smad2 mRNA significantly decreased by 33%,56%,67%,71%,63% and 57%(P

Objective The research aimed to identify symptom clusters of head and neck cancer (HNC) patients.Methods The M.D.Anderson Symptom Inventory-Head & Neck (MDASI-H&N) was applied to measure symptoms of 247 HNC inpatients.Factor analysis was applied to identify symptom clusters.The Spearman correlation analysis was used to find the relationship of symptoms within symptom clusters.Results Five clusters were identified and the Cronbach's α coefficients of symptom clusters were 0.904,0.928,0.898,0.815 and 0.662 respectively.Symptoms within clusters were all significantly interrelated to each other (r=0.393-0.856,P＜0.01).Conclusions Symptoms of HNC patients tend to occur not in isolation,but in symptom clusters.Effective management can not only eliminate or alleviate the symptom burden of HNC patients,but also be cost-effective.

Objective To formulate a scientific and reasonable evaluation model of post competency ,and to provide references for selection ,training and evaluation of orthodontic assistant specialist in china .Methods Items of competency for orthodontic as‐sistant specialist were established basing on semi structured interviewing and literature research ,and then Delphi technique was used to consult 22 orthodontic experts .The collected data was analyzed by SPSS 19 .0 .Results The positive coefficient for two round specialist survey was 95 .45% after two round specialist survey ,the authority coefficient was 0 .865 ,as well as the values of significant test for coordinate coefficient opinions were P<0 .01 .The evaluation competency model for orthodontic assistant special‐ist was consisted of entry requirements and evaluation system including 4primary indicators ,15secondary indicators .Conclusion The evaluation competency model is reliable and valid ,which can provide reference for selection ,training and evaluation of ortho‐dontic assistant specialist .

Objective To explore the role of type Ⅶ collagen (COL7A1) gene in the pathogenesis of pretibial dominant dystrophic epidermolysis bullosa (DDEB-Pt).Methods Peripheral blood samples were obtained from a sporadic Chinese patient of Han nationality with DDEB-Pt,his parents and 100 healthy human controls.A modified salting-out method was used to extract genomic DNA from the blood samples,and PCR was performed to amplify 118 exons of the COL7A1 gene followed by DNA sequencing.Results A G→A mutation was identified at position 6109 (G6109A) in exon 78 of the COL7A1 gene in this patient,which caused a change from GCT to ACT at codon 2037 in the triple helix region,and resulted in the substitution of glycine (Gly) by arginine (Arg) (p.Gly2037Arg).Conclusion A novel glycine substitution mutation was identified in the COL7A1 gene in the patient with DDEB-Pt,which may be a pathogenic mutation.