A simple, rapid and accurate spectrofluorometric method has been developed for determination of vitamin E in crop seeds. Several experiment conditions were discussed in detail. Linear analytical curve was obtained in concentration of 0-55ug of vitamin E in 10 ml solution. The precisions were 0.5-2.9% relative SD. The recoveries were 86-104%.

AIM:To establish the endoplasmic reticulum stress ( ERS) cell model in vascular smooth muscle cells ( VSMCs) of Sprague-Dawley (SD) rats.METHODS:Under sterile condition, the coronary arteries were isolated from SD rats .The primary VSMCs were cultured by tissue-sticking method , and observed the basic morphological characteristics under optical microscope .The marker proteins of VSMCs including α-smooth muscle actin (α-SMA) and smooth muscle myosin heavy chain ( SM-MHC) were identified by immuno-fluorescence technique .VSMCs were treated with thapsigargin (0.5, 1 and 2 μmol/L) for 24 h, and the expression levels of binding immunoglobulin protein (BiP) and C/EBP homologus protein (CHOP), the marker molecules of ERS, were detected using Western blotting.RESULTS:VSMCs climbed out from coronary artery tissues after about six days , and the cells had a nice state and formed the VSMC-like typical peak valley.The results of immunofluorescence technique show that the marker proteins of VSMCs ,α-SMA and SM-MHC were expressed significantly .The results of Western blotting show that the protein expression levels of BiP and CHOP were increased by thapsigargin in a dose-dependent manner .CONCLUSION:VSMCs can be successfully cultured by tissue-sticking method and built the ERS model induced by thapsigargin .

Objective To construct pEGFP-C2-AQP4-M23 plasmid and to detect its expression in CTX-TNA2 cells in order to study the effect of AQP4 on astrocytes in hypoglycemia. Methods AQP4 gene obtained from SD rats by RT-PCR was cloned into pEGFP-C2 plasmid. The recombinant plasmid was transfected into CTX-TNA2 cells, then treated with low glucose. The expression and effect of AQP4 on CTX-TNA2 cells were examed with confocol. Results PCR and enzyme digestion showed AQP4 size was right; confocol demonstrated that AQP4 was expressed on the membrane of CTX-TNA2 cells, and the surfacial area of CTX-TNA2 cells with AQP4was larger than those without AQP4 in hypoglycemia. Conclusion pEGFP-C2-AQP4-M23 plasmid was successfully constructed and expressed in the CTX-TNA2 cell membrane and AQP4 plays an important role in cell edema in hypoglycemia.

Objective:To investigate the safety and efficacy of interleukin-6 antisense oligonucleotide liposomes on the experimental animals for prevention of after cataract. Methods:Thirty healthy New-Zealand rabbits(30 left eyes) received extracapsular lens extraction,and were randomly divided into 3 groups: anterior chamber injection of 0.1ml of interleukin-6 antisense oligonucleotide liposomes(Group A),blank liposomes(Group B) after operation respectively;and control group(Group C).All left eyes were examined by slit-lamp microscope and ophthalmoscope regularly.The aqueous humor IL-6 concentrations were measured 1 day before operation,and 1 day,3 days,7 days,14 days and 30 days after operation through enzyme-linked immunoabsorbent assay.Three months later,photographs of the posterior lens capsules were taken through digital slit-lamp microscope and the histopathological changes of posterior lens capsules were observed by light microscope. Results:①Three months after the operation,the number of eyes developing posterior capsule opacification in Group A,B and C were 7,10 and 10 respectively,with significant difference(P=0.037).② The average value of Group A was lower than Group B and C,with statistically significant difference(P

Vena cava filters (VCF) are extensively used in the prevention of pulmonary embolism, whose performances are closely linked to their structures. As cardiovascular interventional devices,VCFs have to be micro-invasive and have minimized adverse effects. This paper reviewed recent development of vena cava filters and discusses the relationship between their performances and structures.

Vehicle driver fatigue is an important factor in traffic accidents.This paper introduced three kinds of detecting techniques,namely driver's physical signals and individual characteristics,vehicle parameters,and five detecting devices.But there still are limitations of the mentioned detecting techniques and devices in the paper.The problems and developing trend are briefly analyzed.

Objective To explore the contents and modes of community nursing training and improve community care quality.Methods The training course and assessment standard were worked out according to practical work in community health service centers,including basic nursing skills,community first aid skills,hospital infection control skills,preventive health care skills and community document management skills.Toally 58 nurses from 11 community health service centers were trained for 36 hours from January to June,2016 and tested before and after training to assess the effect of training.Result After training,the scores on all skills were significantly higher than those before training (all P＜0.01).Conclusion Regular and practical training and management are the guarantee for the improvement of the community care quality.

Objective To investigate the level of anxiety and emotional intelligence in general nurses of community health service centers in Shenzhen city, and provide theoretical basis for the intervention of anxiety in general nurses by analyzing the relationship between anxiety and emotional intelligence . Methods The self-rating anxiety scale ( SAS ) and emotions intelligence scale ( EIS ) were employed for the survey among 92 general nurses from 20 community health service centers in Shenzhen city . Results The SAS scores was 40.18 ± 7.26, significantly higher than the national norm. The rate of anxiety was 54.35%. Thirty-nine contracted anxiety at a low level, accounting for 42.39%, 10 did at a moderate level, accounting for 10.87%, and 1 did at a higher level, accounting for 1.09%. The EIS score was 3.49 ± 0.47. The highest dimension score was management of emotions for others and the lowest score was emotional perception. The dimension of management of emotions for others was positively related to social anxiety (P<0.05), and the dimension of use of emotions negatively related to social anxiety (P<0.05). Conclusions General nurses of community health service centers in Shenzhen suffer from anxiety at a high level and their emotional intelligence is at a medium level. Both factors predict the level of social anxiety in general nurses of community health service centers . Improving the emotional intelligence can be an approach to reduce the level of anxiety.

Objective To discuss the medical treatment responsibility caused by unpredictable harm.Method In medical treatment,the court usually applies the "Equitable Responsibility" principle to settle the medical conflicts caused by unpredictable harm,this poses certain limitations.Result The fact that hospitals being liable for compensation or reparation ignores the mutual risk-sharing responsibility of hospitals and patients.Conclusion To establish the system of medical accident insurance is one of the best measure to solve the medical conflicts caused by unpredictable harm.

Objective To observe the changes in NO content and NOS activity of serum and the testis in Wistar rats with type I diabetes mellitus. Methods 63 rats were randomly divided into control group(C) and diabetes group(D). Diabetes was induced by intraperitoneal injection of Alloxan. Rats with diabetes were observed for 1 week(D 1), 2 weeks(D 2), 3 weeks(D 3), 4 weeks(D 4), 5 weeks(D 5), 6 weeks(D 6), 7 weeks(D 7) and 8 weeks(D 8), respectively, and they were sacrificed at the end of each observation period. Serum was obtained and testis homogenates were prepared, and the levels of NO content and NOS activity of them were determined respectively. Results Compared diabetes group with control group, NO contents and NOS activity of serum from diabetic rats were higher, then NOS activity began to decline to approach the control level. In the testis homogenates, NO content dropped slightly after injection of Alloxan, while NOS activity showed no significant change. Thereafter, both NO content and NOS activity of testis homogenates were increased. Conclusion There are changes in NO content and NOS activity of serum and testis of rats after diabetes are induced with Alloxan.