Objective To investigate the clinical significance of prealbumin (PA),enzymes,C -reactive protein (CRP)detection in severe hand -foot -mouth disease (HFMD)children with myocardial injury.Methods 183 children with HFMD were selected as the observation group,in which 54 children were severe,129 cases were common.100 healthy children during the same period were selected as the control group.PA,α-acid dehydrogenase (α-HBDH),creatine kinase (CK),creatine kinase (CK -MB),CRP levels were detected in the serum of all sub-jects and compared.Results The serum level of PA in the observation group was (194.37 ±42.86)mg/L,which was significantly lower than (267.15 ±52.04)mg/L of the control group (t =5.942,P <0.05).The serum levels ofα-HBDH,CK,CK -MB and CRP in the observation group were (261.58 ±61.40)U /L,(124.80 ±67.51)U /L, (38.79 ±16.26)U /L and (18.06 ±5.27)mg/L,which were significantly higher than those of the control group [(112.93 ±28.56)U /L,(103.94 ±60.86)U /L,(15.17 ±9.34)U /L and (4.82 ±0.95)mg/L,t =8.356,5.087, 9.446 and 10.755,all P <0.05].The serum PA level of the severe HFMD group was (144.92 ±40.36)mg/L, which was significantly lower than (230.89 ±46.27)mg/L of the ordinary HFMD group (t =7.035,P <0.05).The serum levels of α-HBDH,CK,CK -MB and CRP in the severe HFMD group were (301.58 ±71.40)U /L, (145.31 ±67.84)U /L,(43.66 ±18.95)U /L and (21.86 ±6.55)mg/L,which were significantly higher than those in the ordinary HFMD group [(162.93 ±28.56)U /L,(116.05 ±61.37)U /L,(24.75 ±12.04)U /L and (7.25 ± 3.82)mg/L,t =9.440,6.204,11.038 and 13.118,all P <0.05].The serum PA level of the severe HFMD with myocardial injury group was (131.02 ±36.54)mg/L,which was significantly lower than (149.73 ±42.81)mg/L of the severe HFMD group (t =7.801,P <0.05).The serum levels of α-HBDH,CK,CK -MB and CRP in the severe HFMD with myocardial injury group were (327.84 ±74.95)U /L,(152.92 ±68.75)U /L,(45.17 ±20.68)U /L and (23.07 ±7.46 )mg/L respectively,which were significantly higher than those in the severe HFMD group [(286.30 ±70.16)U /L,(134.80 ±66.31)U /L,(41.08 ±17.23)U /L and (20.95 ±6.20)mg/L,t =4.394, 5.938,4.077 and 4.165,all P <0.05].Conclusion Prealbumin,enzymes,CRP detection is favor of judgement the severity of HFMD children,and conducive to the early diagnosis of myocardial injury in children.

Objective To study the apoptosis of human colorectal cancer HT-29 cells induced by Aralia elata Seem leaf total saponin (ETS) and its effects on the expression of relevant proteins. Methods MTT assay was used to detect the proliferation of human colorectal cancer HT-29 cells cultivated with different concentrations (6.25, 12.5, 25, 50, 100, 200 mg/kg) of ETS. Hoechst33258 staining and laser confocal imaging were used to detect the apoptotic cells. Morphological changes were observed. The expressions of Bcl-2 and Bax were detected by immuno-histochemistry. Results ETS could induce apoptosis of HT-29 cells and apoptosis was in a dose-dependent manner in a certain range. ETS could decrease the expression of Bcl-2 and increase the expression of Bax in HT-29 cells (P<0.05, P<0.01), showing a significant dose-effect relationship. Conclusion ETS can induce the apoptosis of HT-29 cells, and the mechanism may be related to reducing the expression of Bcl-2 and increasing the expression of Bax.