Objective To investigate the association between serum glial fibrillary acidic protein (GFAP) and hypertensive intracerebral hemorrhage(HICH). Methods The level of serum GFAP was detected in 68 patients with HICH by enzyme linked immunosorbent assay(ELISA) and its relationship with bleeding volume,bleeding part,neural deficit score(NDS) and prognosis was analysed, also compared those with the normal control (NC) group. Results Compared with NC group, the levels of serum GFAP in HICH group were increased significantly in 1~14 d after onset (all P20 ng/ml had worse prognosis.Conclusions The level of serum GFAP in patients with HICH is increased significantly, and it is correlate with bleeding volume, condition and prognosis.

BACKGROUND:Stem cel s can be induced to differentiate into dopaminergic neurons in vivo and in vitro, which provides a theoretical basis for stem cel transplantation in the treatment of Parkinson’s disease
OBJECTIVE:To explore the feasibility and mechanism of intracerebral transplantation of umbilical cord blood mesenchymal stem cel s for treatment of Parkinson’s disease rats.
METHODS:Intracerebral injection of 6-hydroxydopamine was used to make Parkinson’s disease models in SD rats. Twenty-two model rats were randomized into cel transplantation group (n=12) and control group (n=10) and respectively injected intracerebral y with umbilical cord blood mesenchymal stem cel suspension and PBS. At 1-8 weeks after cel transplantation, intra-abdominal injection of apomorphine was performed every week to observe the rotation behaviors of rats;at the 2nd and 8th weeks, rat’s striatum and substantia nigra were taken for immunohistochemistry staining.
RESULTS AND CONCLUSION:The rotation behaviors were gradual y decreased with time in the cel transplantation, but had no changes in the control group. At 3-8 weeks after transplantation, there were significant differences in the rotation behaviors between the two groups (P<0.05). At 2 weeks after transplantation, tyrosine hydroxylase-positive cel s were found within and around the striatum of the cel transplantation group;but there were no exogenous cel s in the control group. At 8 weeks after transplantation, there were stil active cel s and tyrosine hydroxylase-positive cel s in the striatum of cel transplantation group, and there was no tyrosine hydroxylase expression in the striatum of the control group. These findings suggest that transplanted umbilical cord blood mesenchymal stem cel s can survive in the brain that are positive for tyrosine hydroxylase, which can improve the behavior abnormalities of Parkinson’s disease rats.

An ultra performance convergence chromatographic ( UPC2 ) method was established for the attribution analysis of the main peaks as well as the quantitative determination of echinacoside andβ-ecdyserone in Bushen Jiannao Grains. The samples were extracted with ethanol and separated on Waters ACQUITY UPC2TM BEH column (100 mm × 3. 00 mm, 1. 7 μm), with a gradient supercritical CO2-0. 05%phosphoric acid-methanol solvent system at 40 ℃. The flow rate was 0. 8 mL/min, the detection wavelength was set at 248 nm and the injection volume was 1 μL. Results showed that all the main peaks in the fingerprint were clearly attributed. The peak named 12 wasβ-ecdyserone with the content of 380μg/g and the peak named 15 was echinacoside with the content of 9. 562 mg/g. The method was simple, eco-friendly, accurate and reliable compared with HPLC and UPLC.

ObjectiveTo observe the lung injury of rats caused by PM2.5 induced imbalance of TH17/Treg immune system and the intervention effect of two different TCM treatments.Methods Wistar male rats were randomly divided into normal group, model group, TCM-treated group1 and TCM-treated group 2. PM2.5-induced lung injury model was established by airway instillation. Model group was given normal saline for gavage. TCM- treated group 1 and 2 were given Yupingfeng Powder and Guomin Decoction combined with Zhisou Powder for gavage. The pathological changes of bronchial and lung tissues, the contents of IL-8, IL-10, IL-17, NE, and MUC5AC in serum and BALF were compared, and the expressions of Foxp3 and IL-17 in lung tissue of each group were analyzed.Results Compared with normal group, the contents of IL-8, IL-17, NE and MUC5AC in serum and BALF of model group increased significantly, while IL-10 decreased significantly (P<0.05,P<0.01); the expression of IL-17 increased significantly and the expression of Foxp3 decreased significantly in lung tissue (P<0.01). Compared with model group, the contents of IL-8, IL-17, and NE decreased in TCM-treated group 1 and 2, while the content of BALF IL-10 increased significantly (P<0.05). The content of IL-10 in serum increased significantly in TCM-treated group 2 (P<0.05); the protein expression of IL-17 of lung issue decreased significantly, and the protein expression of Foxp3 increased significantly (P<0.01). The pathological changes were improved significantly.Conclusion PM2.5 can induce lung injury caused by the imbalance of TH17/Treg. Both two treatments can significantly improve the lung injury induced by PM2.5 and the imbalance of TH17/Tregs immune system.

Objective To establish a simple diabetic peripheral neuropathy (DPN) rat model with the high fat-fed in GK rats. Methods A total of 30 GK rats (7-8 weeks) were fed with high-fat diet to establish the DPN model. Thirty normal Wistar rats were fed with ordinary diet (control group). The blood-sugar value, body mass, water-intake and food-intake were monitored every week in two groups. The serum level of glycosylated hemoglobin, the right sciatic nerve conduction velocity were detected at 8, 12 and 16 weeks respectively. The left sciatic nerve was used for HE and TUNEL staining. Results The manifestations of polydipsia, polyphagia and growth retardation were gradually appeared in GK rats. After 12 and 16 weeks, the blood-sugar and glycosylated hemoglobin were significantly increased in GK rats compared with those of normal Wistar rats (P < 0.01). The sensory nerve conduction velocity decreased obviously (P < 0.01). And motor nerve conduction velocity showed a certain decline trend (12 week P < 0.05,16 week P > 0.05). The sciatic nerve pathological features and Schwann cell apoptosis suggested that the model of DPN was successfully established (apoptosis index, P <0.01). Conclusion GK rats fed by high-fat diet are the satisfactory models of the DPN in experimental research. And 12-week is a suitable and economical time for molding.

Objective To explore the characteristics of emotional identification and emotional intel-ligence of the adolescent methamphetamine addicts,and analyze the correlation between the emotional identi-fication and emotional intelligence.Methods 47 adolescent methamphetamine addicts ( experimental group) and 47 healthy volunteers ( control group) were tested by a self-designed questionnaire,Baron Emo-tional Quotient Inventory ( EQ-i) and experiments of emotional identification.Results The scores of correct responses of positive mood and negative mood of the adolescent methamphetamine addicts ( respectively 28.62±10.60,50.83±18.58) were lower than the healthy volunteers (respectively 32.91±7.30, t=-2.29, P<0.05;62.47±15.75, t=-3.28, P<0.01) .There were significant differences on the sense of responsibility ( re-spectively 33.28±3.79,36.47±4.31;t=-3.81, P<0.01) and impulse control ( respectively 24.81±6.03,30.11 ±5.65;t=-4.40, P<0.01) between the adolescent methamphetamine addicts and the healthy volunteers. While there was no difference on the total score of emotional intelligence between the two groups( t=-0.66, P>0.05) .In the dimension of emotional intelligence,the individual components,the general state of mind,the management of pressure,and the adaptive composition were related to the emotional identification( r 1=0.39, r 2=-0.33, r 3=-0.39, r 4=0.32) .Conclusion The adolescent methamphetamine addicts had some de-fects on the emotional identification and lack of responsibility and impulse control ability.The emotional in-telligence had influence on their emotional identification.

Objective To explore the effect of rational emotion therapy on negative emotional effects such as anxiety and depression in night nurses .Methods 36 night shift nurses were selected as the research subjects . According to the different means of intervention ,the nurses were divided into two groups ,18 cases in each group .The control group was not given psychological guidance , the observation group was given reasonable mood therapy .The negative emotions such as anxiety and depression were recorded in the nurses .Results After intervention,the anxiety self-rating scale,depression self-rating scale of the observation group were (48.05 ±2.54) points and (44.65 ± 2.03)points,respectively,which of the control group were (54.02 ±4.93)points and (48.77 ±4.26)points,respec-tively,the differences were statistically significant (t=4.567,3.704,all P<0.05).After intervention,the depersona-lization score,emotional exhaustion ,personal achievement ,total occupational burnout feeling of the observation group were (10.05 ±1.32)points,(14.43 ±2.13)points,(20.09 ±2.23)points and (44.65 ±3.76)points,respectively, which of the control group were (20.03 ±3.27)points,(34.65 ±5.19)points,(31.83 ±3.87)points,(75.54 ± 8.71)points,respectively,the differences between the two groups were statistically significant (t=12.007,15.291, 11.151,15.291,all P <0.05).The solve problems score,remorse score,help score,fantasy score,retreat score, rationalization score in the observation group were (0.92 ±0.14)points,(0.76 ±0.18)points,(0.88 ±0.19)points, (0.67 ±0.13)points,(0.54 ±0.19)points,(0.85 ±0.21)points,which were higher than those in the control group , the differences between the two groups were statistically significant ( t =9.300,11.392,7.499,15.944,9.104, 15.944,all P <0.05).Conclusion Adopting rational emotion therapy can effectively reduce the psychological resistance of night nurses , relieve psychological pressure , enhance the ability to cope with stress , and improve the well-being of nurses .

Nowadays,the one hospital with multiple districts has become a common model for the development of large public hospitals at present.Through the analysis of the common problems in the development of one hospital with multiple districts,the homogeneous management of one hospital with multiple districts has practical problems,it reflected in difficulties in space management,difficulties,difficulties in staff management,difficulties in system management,difficulties in collaborative development of medical service,difficulties in assessment,difficulties in cost management,difficulties in culture integrations.The case of the scientific management of one hospital with multiple districts in a tertiary public hospitals of provincial state-owned is analyzed,the experience are summarized in terms of organizational structure level,development orientation level,personnel level,performance pay level,cost control level,basic facilities level,culture level,to expected to provide references for promoting the construction of one hospital with multiple districts in public hospitals.

Nowadays,the one hospital with multiple districts has become a common model for the development of large public hospitals at present.Through the analysis of the common problems in the development of one hospital with multiple districts,the homogeneous management of one hospital with multiple districts has practical problems,it reflected in difficulties in space management,difficulties,difficulties in staff management,difficulties in system management,difficulties in collaborative development of medical service,difficulties in assessment,difficulties in cost management,difficulties in culture integrations.The case of the scientific management of one hospital with multiple districts in a tertiary public hospitals of provincial state-owned is analyzed,the experience are summarized in terms of organizational structure level,development orientation level,personnel level,performance pay level,cost control level,basic facilities level,culture level,to expected to provide references for promoting the construction of one hospital with multiple districts in public hospitals.

Nowadays,the one hospital with multiple districts has become a common model for the development of large public hospitals at present.Through the analysis of the common problems in the development of one hospital with multiple districts,the homogeneous management of one hospital with multiple districts has practical problems,it reflected in difficulties in space management,difficulties,difficulties in staff management,difficulties in system management,difficulties in collaborative development of medical service,difficulties in assessment,difficulties in cost management,difficulties in culture integrations.The case of the scientific management of one hospital with multiple districts in a tertiary public hospitals of provincial state-owned is analyzed,the experience are summarized in terms of organizational structure level,development orientation level,personnel level,performance pay level,cost control level,basic facilities level,culture level,to expected to provide references for promoting the construction of one hospital with multiple districts in public hospitals.