1.Study on preparation and identification of monoclonal antibodies immunized with H5N1 influenza virus M1 protein
Ai-Jun CHEN ; Li-Hong YAO ; Jian-Qiang GUO ; Yi XU ; Xiaoy-U LIU ; Run-Ing JIA ; Zhi-Qing ZHANG
Chinese Journal of Experimental and Clinical Virology 2009;23(6):412-414
Objective To prepare monoclonal antibodies specific for M1 protein of H5N1 subtype human influenza virus,this work may provide new tool in rapid diagnosis and study of type A influenza virus.Methods BALB/c mice were immunized with purified recombinant H5N1(A/Anhui/1/2005)/M1 protein expressed in E.coli.Spleen cells of the immunized mice were fused with sp2/0 cells to produce hybridoma cell lines.ELISA was performed to identify the monoclonal antibody against M1 protein of H5N1.Immunofluorescence assay(IFA)were applied to identify the Bpecificity of these antibodies.Results Three hybridoma cell lines steadily secreting anti-H5N1/M1 McAb were obtained,and their cross reactivity was confirmed by cross-reaction test and IfA.Conclusion Monoclonal antibodies immunized with H5N1 subtype influenza virus M1 protein are cross-reactive,which can be used to detect difierent subtype of influenze virus type A.
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