BACKGROUND:Dental pulp stem cel s are characterized by multi-lineage differentiation and proliferation abilities and are easy to obtain, so they are becoming an issue of concern in regenerative medicine. OBJECTIVE:To provide clues and direction for further study by analyzing progress of domestic and overseas research on dental pulp stem cel s, and summarizing their application in regenerative medicine. METHODS:The“dental pulp stem cel , regenerative medicine, tissue engineering”in Chinese and English served as the search terms to search articles related to dental pulp stem cel s and regenerative medicine, published from 2000 to 2015 in Medline, PubMed, CNKI, Wanfang and Cqvip databases. Total y 46 articles were selected for overview. RESULTS AND CONCLUSION:Dental pulp stem cel s, which hold the capacity of self-renewal and multi-lineage differentiation, are relatively easy to obtain, and exhibit a great potential in regenerative medicine. The research of dental pulp stem cel s in repairing bone defects has entered the clinical trial phase, but the research of cel differentiation into other tissues is stil in basic trial phase and needs further development.

Objective To evaluate efficacy of thymopentin combined with ceftriaxone on patients with early syphilis serum fixation.Methods A total of 108 patients with early syphilis serum fixation were chosen and divided into observation group and control group by the number of tables (54 cases in each group).The control group were underwent conventional sodium penicillin with benzathine with nursing treatment,the observation group were treated with ceftriaxone thymopentin combined with nursing treatment,changes in the relevant indicators before and after treat-ment were observed.Results After treatment,IL -2,IL -10 and other indicators in the observation group were improved compared to the control group[IL -2:the control group before treatment (20.8 ±4.9)μg/L,after treatment (42.7 ±7.3)μg/L;the observation group before treatment(19.8 ±5.1)μg/L,after treatment (54.6 ±8.3)μg/L;IL -10:the control group before treatment (76.3 ±16.1)μg/L,after treatment (51.3 ±5.4)μg/L;the observation group before treatment(78.3 ±4.1)μg/L,after treatment(23.8 ±5.1)μg/L],the differences of the two groups after treatment were statistically significant (t =3.923,8.832,all P <0.05);after treatment,the RPR negative rates of the observation group at 3,6,12 months after treatment(72.2%,85.2%,96.3%)was significantly higher than those of the control group(53.7%,57.4%,63.0%),the differences were statistically significant (χ2 =4.932,11.372, 22.842,all P <0.05);the efficacy of the observation group at 3,6,12 months after treatment(75.9%,79.6%, 75.9%)were significantly better than those of the control group (72.2%,64.8%,57.4%),the differences were statistically significant (χ2 =5.232,6.183,all P <0.05 ).Conclusion Thymopentin combined with ceftriaxone treatment of early syphilis can significantly improve its fixed immunological parameters,which coordinated with nursing measures can strengthen patient care awareness,then the efficacy will be better,so it is worthy of clinical further promotion.

Objective To control the quality of Gongyankang granule.Methods Paconiflorin in Gongyankang granule was determined by HPLC,using a Phenomenex Prodigy Thermouest(250×4.6 mm 5μm BDS C18).Mobile phase was consisted of CH_2OH-H_2O-H_3PO_4(30:70:0.1)with the UV detector set at 230 nm.Results The paeoniflorin showed linear correlation(r=0.9998)within the range of 0.01026～0.06156mg.The average recovery rate was 99.12%with RSD of 1.61%.Conclusion This method was simple,accurate and with good reproducibility,thus it can be used for quality control of Gongyankang Granule.

Childhood cancers are malignancies occured during childhood,which consist of blood-lymphatic system disorders and solid tumor,usually under the age of 15 years old.Among cancers of childhood,solid tumors account for the mainly proportion and most neoplasms are embryonic origin,which are distinctly different from the adult solid tumors.The pathogenesis of children with an oncologic disease,except from the 2 major elements heredity and environment,some experts consider that there are intense correlations between various carcinogenic environment exposure during pregnancy even progestation and childhood cancers.The placenta is a combination of embryo and the maternal tissues,while is a material exchanged organ between 2 parts.One part has a same origin with fetus biological and genetics,and the other provides comprehensive guarantee for fetus normal development in utero.The research of the placental disease of childhood cancers may have a breakthrough in the study of the etiology of pediatric oncology.

BACKGROUND:Individuals with different vertical facial types have different chin morphologies. For the individuals with different vertical facial types, what is the most beautiful chin morphology?
OBJECTIVE:To evaluate the effects of vertical facial types and chin morphology on facial profile attractiveness.
METHODS:Three beautiful females were selected, including one with high mandibular angle, one with average angle and one with low angle. Their facial profile photographs were taken. A series of new pictures were generated by the smal scale (2 mm per unit) modification of soft tissue chin in the sagittal direction and vertical direction. Raters consisting of 17 orthodontists and 35 laypersons were selected for aesthetic ratings, in order to evaluate the facial profile attractiveness of the individuals with different vertical facial types.
RESULTS AND CONCLUSION:There was no esthetic difference of vertical change in female with high angle;while slightly protrude chin was perceived to be more beautiful than retruded chin. For the female with average angle, lower chin was more attractive than higher chin;slightly protruded chin was beautiful than excessive protruded chin and retruded chin. For the individual with low angle, higher chin was preferred by raters;slightly protruded chin was more beautiful than excessive protruded chin and retruded chin.The results indicate that the effects of vertical facial types and chin morphology to facial profile attractiveness are different. In clinical orthodontic, vertical facial types should be taking into consideration to change the chin morphology of the patents, in order to obtain better profile attractiveness.

Objective To investigate the association of thyroid carcinoma and urine iodine level in Urumqi region,intent to provide the evidence of individual iodine supplement of this region.Methods In this case-control study,there were 100 patients with thyroid carcinoma and 300 patients with normal thyroid function as the control group in Xinjiang Tumor Hospital between February 2012 and December 2012 as the case group.All the cases were detected for urine iodine concentration by arsenic-cerium catalytic spectrophotometry.Results The mean level of urinary iodine in the case group (272.69 μg/L) was higher than that in the control group (161.40 μg/L) (P ＜ 0.05).The level of iodine was excessive (urinary iodine ＞ 300 μg/L) in 34 ％ (34/100) patients in the case group and 10 ％ (30/300) patients in control group (P ＜ 0.05).Conclusion The average urinary iodine content of the thyroid carcinoma in Urmuqi region are generally higher than that of the healthy control group,which indicates that the iodine nutritional status in patients with thyroid carcinoma are better than that in the general population.The relationship between thyroid carcinoma and excessive iodine intake should be necessary for further study.

This paper introduces the ways to conduct the fixed denture teaching in prosthodontics with the multivariate teaching and learning mode,including information technology and PBL teaching,simulation model system,knowledge integration and the increasing of clinical practice.

Objective To investigate the effect of rectal washout on prevention of local recurrence in patients with resectable rectal cancer.Methods Relevant studies published on PubMed,Embase database and Google between January 1980 and June 2013 were identified and reviewed.A meta-analysis was performed to compute the risk ratio (RR) and evaluate the effect of rectal washout on local recurrence in patients included in the reviewed studies.Results Eight studies were included in the meta-analysis,in which a total of 427 patients underwent rectal washout and 492 did not during rectal cancer resection.The patients had an overall local recurrence (LR) rate of 6.64 ％ (61/919).Rectal washout,no matter which type of washout solution was used,significantly reduced the risks of local recurrence (3.75 ％,16/427 vs 9.15 ％,45/492,RR =0.46,95 ％ CI =0.26-0.82,P =0.008).Patients with rectal washout showed a low LR rate,when compared with those in the three subgroups who underwent total mesorectal excision (TME) (5.18 ％,10/193 vs 5.32 ％,10/188).However,there was no statistically significant difference in the risk of LR among the three subgroups (RR =0.99,95 ％ CI =0.42-2.30,P =0.98; RR =0.44,95 ％ CI =0.18-1.04,P =0.06; RR =0.46,95 ％ CI =0.20-1.04,P =0.06,respectively).Conclusions The intra-operative rectal washout largely reduces local recurrence after anterior resection of rectal cancer.Rectal washout should be applied routinely during anterior resection.

Objective To explore the clinical application value of blood routine examination in judgment of the activity of inflammatory bowel diseases.Methods 102 patients with inflammatory bowel disease were selected as the main research objects,60 healthy persons in the same period were selected as the control group,all of the subjects were received routine testing of blood,C-reactive protein and erythrocyte sedimentation rate,and the relationships between disease and blood routine indexes were compared.Results In UC group,the PLT,WBC RDW index levels of remission were higher than those of the control group,the differences were statistically significant(t =6.75,8.21,5.73,all P ＜0.05).In patients with UC,the HGB,HCTand MPV index levels were lower than those in the remission patients and the control group,the differences were statistically significant(t =8.12,11.03,6.72,9.35,10.23,8.71,all P ＜0.05).But the PLT index was higher than that of patients in remission,the RDW and WBC indexes were higher than those in the control group and the remission period,there were statistically significant differences (t =7.59,8.51,6.03,all P ＜ 0.05).The routine blood indexes of the relief patients of CD group had significant differences compared with the control group(t =6.17,7.29,9.01,10.35,8.27,7.69,8.01,9.91,allP ＜ 0.05),the HCT,MCV,HGB and MPV indexes were lower than those of the control group,the differences were statistically significant (t =9.01,10.35,8.27,9.9i,all P ＜ 0.05),the WBC,PLT,N and RDW indexes were higher than those of the control group,the differences were statistically significant (t =6.17,7.29,7.69,8.01,all P ＜ 0.05).In UC group,the HGB,HCT,MCV and MPV indexes were lower than those in the remission patients and the control group,there were statistically significant differences(t =8.19,6.97,7.53,9.02,all P ＜ 0.05).But the PLT,RDW,WBC and N indexes were higher than those in the remission patients and the control group,the differences were statistically significant (t =9.81,7.94,8.36,7.61,P ＜ 0.05).In the active patients of CD group,the WBC and N indexes were significantly higher than those of UC group,the differences were statistically significant (t =8.15,9.07,all P ＜ 0.05).In the remission patients of CD group,the PLT index was significandy higher than that in the remission patients of UC group,the difference was statistically significant (t =10.35,P ＜ 0.05).Conclusion Indexes of blood routine has obvious significance in activity of inflammatory bowel disease,and it is significant correlation with inflammation index,which can be used as one of the routine clinical method to determine the activity of inflammatory bowel diseases.

Objective To study the influence of sunscreens with different efficacy on delayed type hypersensitivity (DTH) and their immunoprotective effect in mice.Methods A cohort of mice were randomly divided into 5 groups with 10 mice in each group:group 1 as the positive control without irradiation,group 2 receiving solar-simulated radiation (SSR) only,group 3 receiving SSR and protected by sunscreen l with sun protection factor 15(SPF15)and persistent pigment darkening(PPD)12,group 4 receiving SSR and protected by sunscreen 2 with SPF 50 and PPD 28,and group 5 as the negative contml receiving SSR only.SSR was carried out on the back of mice with the UVA dose being 1.4 J/cm2 and UVB dose being 100 mJ/cm2 for 10 days.After a 5-day irradiation,the groups 1 to 4 were immunized by intraperitoneal injection with 100 μl(107 cells/ml) of Candida albicans suspension.On the 10th day both sides of the posterior foot pad were measured;then the foot pads were injected with additional 50 μl of the Candida albicans suspension.Twenty-four hours after the injection,the thickness of each foot pad was measured,and immunosuppression rate was calculated.Finally,the mice were sacrificed and skin samples were obtained from the back of these mice followed by the examination of CDla, CD80 and CD86 expression by Western blot.Resets The thickness of edema in foot pads was 0.41±0.38 mm,0.21±0.23 mm and 0.30 ± 0.25 mm in group 1,3 and 4,respectively,significantly higher than in group 5 and 2(0.04±0.03 mm,0.14±0.12 mm,respectively,all P<0.05),while no significant difference was observed between the group 3 and 4(P>0.05).Significant differences were observed in the immunosuppression rate between group 2,3 and 4(73.0%±11.3%,54.1%±6.4%,29.7%±7.5%,respectively,all P<0.01).Western blot revealed a significant increment in the expression of CDla protein in group 1 compared with group 2 as well as in the expression of CD86 protein in group 1 and group 3 compamd with group 2 and group 5(all P<0.05),but no statistical difference was observed between the other groups in the expression level of CDla,CD80 or CD86(P>0.05).Conclusions The exposure to sub-erythema dose of UV can induce DTH,and sunscreens have an immunoprotective effect in this process.Epidermal Langerhans cells are not essential for UV-induced immunosuppression.