Objective To investigate the effect of ademetionine assisted therapy on transferrin ( TRF) , myocardial enzyme spectrum and bilirubin in serum of patients with icterus neonatorum.Methods 86 cases of neonatal jaundice in the hospital were randomly divided into two groups.43 cases in control group were treated with conventional therapy, 43 cases in experimental group were treated by ademetionine.Serum transferrin, myocardial enzymes, serum total bilirubin levels and clinical effects were compared pre-treatment and post-weat ment.ResuIts Compared with control group, the TRF level of experimental group was higher (P<0.05), the level of Aspartate transaminase (AST), lactate dehydrogenase (LDH), creatine kinase (CK), and CK-MB in experimental group were lower (P<0.05), the total bile acid (TBA), total bilirubin (TBIL) and direct bilirubin (DBIL) in experimental group were lower (P<0.05).The total effective rate in experimental group was significantly higher than that in control group (χ2 =8.53, P<0.05).ConcIusion Ademetionine assisted therapy has a good clinical curative effect and could effectively reduce serum TRF, DBIL and myocardial enzyme spectrum level, which has important significance in the treatment of neonatal jaundice.

0.05) were seen among the cases given autohemotransfusion including hemodilution and the use of retrieved blood comparing with the allogeneic transfusion. Therefore, hemodilution and retrieving blood for retransfusion are safe and reliable ways to be adopted in orthopaedic surgery.

Objective:To study the bactericidal effect of ultrasonic technique combined with saline(SL),sodium hypochlorite(SH) or Koutai(KT) irrigation in root canal preparation. Methods:120 single-rooted teeth in 120 patients with chronic periapical periodontitis were divided into six groups randomly with 20 in each group.The tooth canal in group A, B and C were prepared with ultrasonic technique. SL, SH and KT were used to irrigate in the root canals group A,B and C respectively.Routine hand instruments were used in group A1, B1 and C1.SL, SH and KT were used as irrigations in group A1, B1 and C1 respectively.30 ?l of liquid sample was obtained from each root canal and examined for anaerobic bacteria colony counting before and after root canal preparation. Result:After root canal preparation the number of anaerobic bacteria colony was significantly reduced in all the 6 groups(P

Objective To study the inhibitory effect of recombinant adenoviruses carrying the mouse endostatin gene on the therapy of endometriosis in nude mice.Methods Animal endometriosis models were established by inoculating human endometrial fragments.Mice were randomly divided into three groups(10 each):PBS control group,Ad-lacZ group and Ad-mEndo therapy group when ectopic lesion diameter reached 5-10mm.The ectopic lesion size was measured every day.The expression of endostatin in ectopic lesion was detected by immunohistochemical stainining.The microvessel density(MVD) of ectopic lesion was traced by CD34 immunohistological labeling.Meanwhile,the contents of steroid hormone and histological changes in mice uterus and ovary were detected.Results The titer of the recombinant adenovirus was about 5.0?109pfu/ml.The endostatin gene was transferred into mice successfully and expressed effectively.The lesion volume was significantly less in Ad-mEndo group(34.3?11.2mm3) than that in Ad-lacZ group(93.3?10.7mm3) and PBS group(90.4?18.7mm3,P

Objective To investigate the latest situation in the period 2006-2008 of drug resistance tuberculosis among migratory population in Chaoyang district of Beijing.Methods All sputum culture-positive specimens from migratory population were collected.Drug susceptibility of Mycobacterium tuberculosis to M,H,R and E were tested by proportion method.Results Drug susceptibility of 371 isolates from 2006-2008 were tested.Total drug resistance wag 22.4%(83/371),the primary drug resistance and the acquired drug resistance were 17.5%(58/331)and 62.5%(25/40)respectively.The multi-drug resistance was 5.9%(22/371);the primary multi-drug resistance was 4.2%(14/331)and acquired multi-drug resistance wag 20.0%(8/40).The drug resistance and multi-drug resistance were higher in treated cases than that in new cases.and the difference wag statistically significant(χ~2 was 39.020 and 13.210,P<0.01).The drug resistance to 4 anti-tuberculosis drugs from high to low was M(16.7%).H(12.1%),R(11.3%),E(1.9%).The primary drug resistance rate were 13.3%(S),8.8%(H),8.2%(R),1.8%(E).The acquired drug resistance rate were 45.5%(S),40.0%(H),37.5%(R),2.5%(E).The drug resistance to S,H and R were higher in treated cases than that in new cases,and the difference was statistically significant(χ~2 was 23.549,29.810 and 27.754,P<0.01).Conclusion The drug resistance to mycobacterium tuberculosis among migratory population was relatively high in chaoyang district of Beijing,suggesting the necessity to strength the tuberculosis control program for migratory population.

Objective To investigate the effect of transcutaneous electric acupoint stimulation (TEAS)of Hegu-Neiguan-Houxi-Zhigou for preventing postoperative nausea and vomiting (PONV) in patients undergoing thoracoscopic lobectomy.Methods Sixty-four ASA physical status Ⅰ-Ⅲ pa-tients,aged 18-75 years,scheduled for elective thoracoscopic lobectomy were enrolled.Patients were randomly allocated into two groups (n =32):TEAS group (group E)and sham TEAS group (group C).TEAS at Hegu,Neiguan,Houxi and Zhigou was applied to patients before,during and after sur-gery in group E.The frequency was 2/100 Hz and the intensity was 2 times the threshold of sensation before and after surgery,and 30 mA during surgery.While sham TEAS with the intensity of sensory threshold was given to patients before and after surgery in group C.Patients in both groups received sufentanil,propofol and rocuronium for induction of anesthesia.Target controlled infusion of propofol and remifentanil was administered for maintenance of anesthesia.Patient-controlled intravenous anal-gesia (PCIA)was applied in both groups.No antiemetic was given to patients before and during sur-gery.Frequency of nausea or vomiting and any use of antiemetic for remediation were recorded after surgery.The postoperative Visual Analogue Scale (VAS)score and analgesic dosage were also docu-mented.Results Compared to group C,the incidence of nausea immediately after surgery,6 h after surgery,24 h after surgery and 48 h after surgery were significantly lower in group E.The incidence of vomiting 24 h after surgery was significantly lower in group E.The VAS score 6h and 24 h after surgery were significantly lower in group E.The analgesic dosage 48 h after surgery were reduced in group E.There were no significant differences regarding the use of antiemetic for remediation between the two groups.Conclusion TEAS at Hegu-Neiguan-Houxi-Zhigou is effective for preventing PONV in patients undergoing thoracoscopic lobectomy.

Objective To analyze the projects funded by National Natural Science Foundation of China(NSFC) in the field of liver diseases during 2010 to 2014.Methods The projects in the field of liver disease were statistically analyzed regarding the subordinate branches and distribution of the applicants and registered institutions.Results 1.There were 624.4 projects and 321,839,200 yuans annual funded in the field of liver diseases; 2.The percentage of the key/major projects was lower than the average level; 3.Nearly half of the projects funded focus on the liver cancer; 4.19.03％ of registered institution got 80％ of funded projects.Conclusions With the continuous funding aid from NSFC,the fundamental research in this field has progressed significantly,especially in immunology.However,the overall research capacity still need to be further improved and the research direction need to be connected more closely to the clinical requirement to meet the translational medicine demand.

Objective To study the mechanism of hypoxia inducing factor-1α(HIF-1α)pathway in establishment of hypoxia inducing low endometrial receptivity.Methods RL95-2 cell lines.the ideal model of study ER,were cultured in hypoxia condition induced by CoCl2,and the expression of mRNA and protein of HIF-1α and tumor necrosis factor like weak inducer of apoptosis(TWEAK)were measured by reverse transcription-PCR and western blot. The apoptosis rate was analyzed by flow eytometry.Then the mechanism confirmed by comparing the two factors in endometrium and the ultra-appearance of inflammatory reaction and apoptosis between recurrent spontaneous abortion women and control women.Results (1)On difierent time point(0,12,24,48 hour),mRNA expression of HIF-1α were 0.272±0.010,0.354±0.020,0.591±0.020.0.890±0.020,while the expression of TWEAK were 0.104±0.010,0.510±0.020,1.021±0. 020, 1. 237 +0. 040, respectively, the expression level between 12, 24, 48 and 0 hour all showed significant differences (P＜0. 05 ). (2) Protein expression of HIF-1α were 0. 853 +0. 010, 0. 931 ±0. 030,1. 124±0.010, 1.317±0.0 20 respectively, while was 0.042±0.010, 0.091 ±0.010, 0. 131±0.020,0. 205 ±0. 030 in TWEAK expression, the different level were statistically significant ( P＜0. 05 ). ( 3 )With longer culture under hypoxia, the cell apoptosis rate increased obviously. The apoptosis rate of each time point were ( 3.2±1.4 ) %, ( 16. 2 ±3.2 ) %, ( 26. 3±3.5 ) %, ( 31.8±3.5 )%, the differences between 12, 24, 48 and 0 hour had significance (P ＜0. 05). (4) The positive rate of HIF-1α stained in epithelium cells and stroma cells of test group were 32. 3%, 8.4% and 16. 7%, 7. 3% in control group. The positive rate of TWEAK were 28. 3%, 3.9% in recurrent spontaneous abortion group and 11.6%, 2. 7% in control group ( P ＜0. 05 ). The ultra-appearance of inflammatory cell infiltrated and apoptosis were obvious in test group. Conclusions Cell inflammation reaction and apoptosis induced by HIF-1α pathway may participate the mechanism of hypoxia inducing low endometrial receptivity. HIF-1α might become a novel target for improving poor endometrial receptivity.

Objective To conduct the diagnostic treatment in the children under 5 years old with suspected asthmatic disease and to evaluate its safety .Methods The children outpatients with suspected asthmatic diseas were selected and divided into the experi‐mental group and the control group according to odd days and even days .The conventiona therapy of anti‐infection ,relieving cough , reducing sputum and supporting treatment were administered in both groups .The experimental group was added tulobuterol tape . The cough relieve on daytime and nighttime ,initial effective time ,cough disappearance time and occurrence rate of adverse events at 24 ,72 h and on 5 d after treatment were compared between the two groups .Results The total effective rate of cough sysmptom re‐lief in the experimental group at 3 time points and nighttime was higher than that in the control group with statistical difference be‐tween the two groups(P < 0 .05) .The initial effective time had no statistically significant difference in daytime between the two groups(P> 0 .05) ,but which in daytime showed the statistically significant difference (P < 0 .05) ,especially the cough disappear‐ance time at nighttime in the experimental group was significantly shortened than that in the control group( P < 0 .05) .The oc‐curence rate of adverse events had no statistical difference between the two groups(P > 0 .05) .Conclusion Tulobuterol tape by transdermal delivery as one of diagnostic treatment measures for children under 5 years old with suspected asthmatic disease is defi‐nitely effective ,safe and convenient in application with the high compliance for children patients and acceptability by the family members ,obviously shortens the course of treatment ,which is worthy of being promoted .

Objective To investigate the effect of FCGR3A polymorphisms on NK cell function. Methods Peripheral blood samples from can?cer patients were collected and FCGR3A polymorphisms were confirmed by PCR. In vitro proliferation rates,ADCC activity,and expression of NK cell activating receptors were compared under trastumab stimulation. Results This study showed that the wild?type FCGR3A exhibited a higher affinity to trastumab along with better NK cell proliferation and ADCC activity than the mutant type. Compared to the patients with wild?type FC?GR3A,the proliferation rates of NK cells in patients with the mutant type were reduced by approximately 8?fold. In addition,the expression of NK cell activating receptors in patients with wild?type FCGR3A was higher than in patients with the mutant type. Conclusion Mutations in FC?GR3Areduce NK cell function,causing a poor reaction to monoclonal antibody.