Objective To prepare RGD conjugated paclitaxel and curcumin loaded liposome and evaluate their properties and effect on the treatment of ovarian carcinoma in vitro and in vivo . Method The liposomes were prepared by thin film hydration method. The particle size,Zeta potential and entrapment efficiency were evaluated. The efficiency of cellular uptake on A 2780 cells in vitrowas evaluated. Flowcytometry were used to observe the apoptosis morphous.A 2780 cells werexenografted in athymic mice to establish the animal model, which were used to evaluate the effect of anti-cancer. Results The particle diameter of the RGDLP-PTX/Cu was (94.8±11.2) nm with the Zeta potential of (-3±2.45) mV. The entrapment efficiency of PTX and Gen were 81.8%and 84.6%respectively. The result demonstrated that RGDLP uptaken by A 2780 were 3.6 times higher than that of LP. The apoptosis assay、the inhibition of the inhibition of tumor in vivoconfirmed strong inhibitory effect ofRGDLP-PTX/Cu. Conclusion RGDLP-PTX/Cu were easy to prepare and it is a potential delivery system for the treatment of ovarian carcinoma.

Adaptive response is a topic of general interest in medical research. Utilization of adaptive response has become a new treatment strategy for some diseases, the core of which is to stimulate the mechanisms of body internal protection, to improve the adaptive and anti-injury capability, and to maintain the homeostasis of the body. The existence of adaptive response indicates that the body internal protection can be induced and its key mechanism is the inducement of internal protective factors (internal protective proteins). In this article, some connections between adaptive response and the theory of traditional Chinese medicine (TCM) were discussed, which indicated that adaptive response is much beneficial for explaining some theories of TCM such as correspondence between human and nature, strengthening healthy qi to consolidate constitution, acupuncture theory, adaptogen herbs, and treating disease before its onset, etc. Thus the adaptive response is of great significance for explaining scientifically the connotative meaning of the theories of TCM and for promoting the integration of TCM and western medicine.

Objective To compare the effect of the McKenzie method and traditional manipulation in the treatment of patient with cervical spondylotic radiculopathy. Methods Seventy-two patients (42 women and 30 men with an average age of 42.60?13.73 years ) were randomly divided into 2 groups: an observation group and a control group. Both groups were given cervical traction, medium frequency electrotherapy and ultrasound therapy. In addition, the observation group was also treated with the McKenzie techniques, while the control group was treated by traditional manipulation. All the subjects were evaluated in terms of the clinical effect, VAS(Visual analog scale), McKenzie′s movement loss. The 1-year follow-up results were obtained. Results Both groups got a good therapeutic effect( the cure rate was 69% and 55.5%, respectively) and significant pain relief (P0.05). Range of motion of the cervical spine of the observation group was significantly improved after the treatment(P

Objective To observe the effects of fluoride on testicular cell cycle and cell apoptosis of male rats.Methods Thirty-two healthy male Wistar rats,weighting 150-180 g,were randomly divided into 4 groups by body weight using random number table,normal sodium (control),the low-dose,medium-dose and high-dose groups(100,200,300 mg· kg-1· d-1 NaF,respectively) by intragastric administration for 90 days,and bodyweight was observed daily.After the last intragastric administration,all rats were killed by cervical dislocation.The testicular cell cycle and cell apoptosis were measured by flow cytometry.Results After 30 days exposure,the difference of body weight between groups was statistically significant(F =3.884,P ＜ 0.05).The body weights in low-and medium-dose groups[(235.00 ± 14.56),(235.44 ± 24.99)g] were significantly increased than that of high-dose group [(206.00 ± 18.16)g,all P ＜ 0.05].There was no significant difference of body weight between the groups at 0,60 and 90 days(F =0.501,0.578,1.893,all P ＞ 0.05).Compared with the control group[(43.10 ± 3.62)％],the percentages of G0/G1 stage cells were significantly increased in all the NaF-treated groups [(57.60 ± 7.26) ％,(52.80 ± 3.20) ％,(73.13 ± 4.08) ％] and the percentages of S stage cells were significantly decreased in all the NaF-treated groups [(10.58 ± 2.58)％,(9.35 ± 0.35)％,(9.55 ± 0.50)％] compared to the control group[(19.23 ± 0.61)％,all P ＜ 0.05].On the other hand,the percentage of G2/M stage cells decreased significantly in high-dose group[(17.18 ± 2.21)％] compared with the control group[(36.34 ± 5.05)％,P ＜ 0.05].The testicular cell apoptosis ratios in all the NaF-treated groups were higher than that in the control group,but only in medium-and high-dose groups[(71.03 ± 2.30)％,(71.90 ± 2.16)％],the difference was statistically significant compared with the control group [(60.80 ± 2.34)％,all P ＜ 0.05].Conclusion Chronic fluorosis can change testicular cell cycle and cell apoptosis and damage the reproductive system.

OBJECTIVE:How to explain and predict individual difference of artificial cochlea so as to evaluate rehabilitative effect of implant has been paid much attention by audiological scholars,clinical physicians,patients,and their relatives. This study was designed to analyze and evaluate rehabilitative status of patients accepting artificial cochlea implantation to investigate the relative influential factors of auditory rehabilitation. METHODS:A total of 28 patients were selected from Department of Otolaryngology Head and Neck,Affiliated Hospital of Qingdao Medical College from June 2002 to April 2008. They were accessed by age,time of rehabilitation training,usage of hearing aids,audiology and aural image before surgery,and NRT monitoring in cochlear implantation so as to observe auditory improvement. The clinical results were analyzed to detect the effect of various factors on auditory rehabilitation. RESULTS:Age,preoperative preparations and good audiology and aural image are important premises for a successful rehabilitation. Longer the time of using hearing aids and language rehabilitation are,and better results will get. NRT response during operation is also an important factor for good auditory rehabilitation. CONCLUSION:The influential factors of auditory rehabilitation after cochlear implant included the age,audition remains,usage of hearing aids,NRT response during operation,and the time after the cochlear implant operation.

Objective:To investigate the clinical efficacy and outcomes of two separate vertical wiring combined with tension band and Kirschner-wire plus cerclage wire in the treatment of displaced inferior pole fractures of the patella.Methods:From January 201 3 to January 201 5,1 5 consecutive patients (mean age 54.5 years)with inferior pole fractures of the patella were retrospectively included in this study.All the patients underwent open reduction and internal fixation by separate vertical wiring com-bined with tension band and Kirschner-wire plus cerclage wire through longitudinal incision,4.5 d (range:3.1 -5.9 d)after initial injury.A safety check for early knee range of motion was performed before wound closure.The complications including infection,nonunion,loss of fixation and any wire breakage or irritation from implant were recorded.Anteroposterior and lateral views of the knee joint ob-tained during the follow-up were used to assess bony union based on the time when the fracture line disap-peared.At the time of the final outpatient follow up,functional evaluation of the knee joint was conduc-ted by B?stman system.Results:The follow-up time was 1 3.1 months (range:1 2 -1 9 months)after surgery on average,immediate motion without immobilization in all the cases was allowed and there was no case of reduction loss of the fracture and wire breakage.There was no case of irritation from the im-plant.At the final follow-up,the average range of motion (ROM)arc was 1 26.7°(range:1 1 5°-1 40°),the average ROMlag versus contralateral healthy leg was 1 0.3°(range:0°-35°).The mean B?stman score at the last follow-up was 28.9 (range:27 -30),and graded excellent in most cases. Conclusion:Two separate vertical wiring is an easy and effective method to reduce the displaced inferior pole fracture of patella.Augmentation of separate vertical wiring with tension band and Kirschner-wire plus cerclage wire in these patients provides enough strength to protected the early exercise of the knee joint and uneventful healing.By this surgical treatment,excellent results in knee function can be expec-ted for cases of displaced inferior pole fractures of the patella.

Objective To investigate the risk factors of acute exacerbation of chronic obstructive pulmonary disease(AECOPD) combined with respiratory failure.Methods The clinical data of 120 patients with AECOPD combined with respiratory failure were retrospectively analyzed.The patients were divided into disease improved group (96 cases) and disease serious group (24 cases,including death) according to the treatment outcome.Risk factor and treatment strategies of the two groups were statistically analyzed.Results There was no significant difference in age,sex,disease course and arterial blood oxygen partial pressure(P ＞ 0.05).The body mass index,albumin,pH,successful cough and expectoration rate,nutritional support rate,and noninvasive mechanical ventilation rate in disease improved group was higher than that in disease serious group[(28.5 ± 9.8) kg/m2 vs.(23.3 ± 7.4) kg/m2,(21.4 ± 7.9) g/L vs.(19.3 ± 6.8) g/L,7.23 ± 0.20 vs.7.11 ± 0.17,79.2％ (76/96) vs.25.0％ (6/24),58.3％ (56/96) vs.33.3％ (8/24),81.2％ (78/96) vs.62.5％ (15/24)],arterial blood carbon dioxide partial pressure,mild consciousness obstacle rate,multiple drug-resistant infections rate,fungal infection rate,nasal feeding rate,hormone use time and the incidence of heart failure,pulmonary encephalopathy,hepatorenal function damage and upper gastrointestinal bleeding in disease improved group was lower than that in disease serious group [(10.08 ±1.71) kPa vs.(11.98 ± 2.03) kPa,13.5％ (13/96) vs.58.3％ (14/24),22.9％ (22/96) vs.41.7％ (10/24),0 vs.29.2％ (7/24),50.0％(48/96) vs.75.0％(18/24),(5.3 ± 1.3) d vs.(11.3 ± 3.8) d,8.3％(8/96) vs.25.0％(6/24),13.5％(13/96) vs.70.8％(17/24),28.1％(27/96) vs.41.7％(10/24),5.2％(5/96) vs.12.5％(3/24)],and there was significant difference (P ＜ 0.05).Conclusion Complicating disease,nutritional status,consciousness,capability of sputum exclusion,the degree of respiratory infection,treatment with noninv asive positive pressure ventilation,arterial gas analysis index are the correlated factors of prognosis in AECOPD combined with respiratory failure.

Objective To study the relationship between abnormal blood pressure distribution characteristics and the influencing factors for the prevention of hypertension and low blood pressure to provide reference. Methods Regarding the Guangzhou City adult group as the research object,we analyzed the distribution of the adults abnormal blood pressure in age, gender , urban and rural areas, as well as of occupations. Results The detection rate of blood pressure abnormalities was 15.9 % , which accounted for 12.4% of hypertension, 3.5% of hypotension. On the whole, the detection rate of hypertension was 18% in rural, higher than 9.8% in urban. The detection rate of hypotension in rural areas was 2.3% , 4.0% in urban. The detection rate of hypertension of men was high than that of women, and the detection rate of hypertension gradually increased by the age increased. At the same time, hypertension and hypotension was significantly affected with the factors of education, urban and rural areas, obesity and so on. Conclusion The detection rate of hypertension is high in rural areas than that in urban,and men than women, and the detection rate of low blood pressure is on the opposite. Both of them is significantly affected by the factors of education, urban and rural areas, obesity and so on.

The vectors play an important role in gene therapy by transporting the therapy genes into target tumor cells. At present there are mainly two kinds of gene vectors, including biological and non-biological vectors. Biological vectors, including virus, bacteria and stem cells, have good target activity and can efficiently delivery the genes into target tumor cells, but have potential genetic hazards.However, non-biological vectors, including liposomes and nanoparticles, can be simplely prepared with high genetic safety, but they can not transport the genes efficiently. Therefore, establishing safe and effective malignant glioma gene treatment system and finding gene vectors with good target activity become the research focus.

Recently, the incidence of both diabetes mellitus and diabetic foot has been increasing.Ischemia, neuropathy, and infection are major causes of diabetic foot ulcer. In addition to other conventional treatments, hyperbaric oxygen is an effective adjunctive therapy with less side-effects. Hyperbaric oxygen may play a role through different mechanisms in improving the prognosis of diabetic foot.