Adaptive response is a topic of general interest in medical research. Utilization of adaptive response has become a new treatment strategy for some diseases, the core of which is to stimulate the mechanisms of body internal protection, to improve the adaptive and anti-injury capability, and to maintain the homeostasis of the body. The existence of adaptive response indicates that the body internal protection can be induced and its key mechanism is the inducement of internal protective factors (internal protective proteins). In this article, some connections between adaptive response and the theory of traditional Chinese medicine (TCM) were discussed, which indicated that adaptive response is much beneficial for explaining some theories of TCM such as correspondence between human and nature, strengthening healthy qi to consolidate constitution, acupuncture theory, adaptogen herbs, and treating disease before its onset, etc. Thus the adaptive response is of great significance for explaining scientifically the connotative meaning of the theories of TCM and for promoting the integration of TCM and western medicine.

Objective To prepare RGD conjugated paclitaxel and curcumin loaded liposome and evaluate their properties and effect on the treatment of ovarian carcinoma in vitro and in vivo . Method The liposomes were prepared by thin film hydration method. The particle size,Zeta potential and entrapment efficiency were evaluated. The efficiency of cellular uptake on A 2780 cells in vitrowas evaluated. Flowcytometry were used to observe the apoptosis morphous.A 2780 cells werexenografted in athymic mice to establish the animal model, which were used to evaluate the effect of anti-cancer. Results The particle diameter of the RGDLP-PTX/Cu was (94.8±11.2) nm with the Zeta potential of (-3±2.45) mV. The entrapment efficiency of PTX and Gen were 81.8%and 84.6%respectively. The result demonstrated that RGDLP uptaken by A 2780 were 3.6 times higher than that of LP. The apoptosis assay、the inhibition of the inhibition of tumor in vivoconfirmed strong inhibitory effect ofRGDLP-PTX/Cu. Conclusion RGDLP-PTX/Cu were easy to prepare and it is a potential delivery system for the treatment of ovarian carcinoma.

Objective To compare the effect of the McKenzie method and traditional manipulation in the treatment of patient with cervical spondylotic radiculopathy. Methods Seventy-two patients (42 women and 30 men with an average age of 42.60?13.73 years ) were randomly divided into 2 groups: an observation group and a control group. Both groups were given cervical traction, medium frequency electrotherapy and ultrasound therapy. In addition, the observation group was also treated with the McKenzie techniques, while the control group was treated by traditional manipulation. All the subjects were evaluated in terms of the clinical effect, VAS(Visual analog scale), McKenzie′s movement loss. The 1-year follow-up results were obtained. Results Both groups got a good therapeutic effect( the cure rate was 69% and 55.5%, respectively) and significant pain relief (P0.05). Range of motion of the cervical spine of the observation group was significantly improved after the treatment(P

OBJECTIVE:How to explain and predict individual difference of artificial cochlea so as to evaluate rehabilitative effect of implant has been paid much attention by audiological scholars,clinical physicians,patients,and their relatives. This study was designed to analyze and evaluate rehabilitative status of patients accepting artificial cochlea implantation to investigate the relative influential factors of auditory rehabilitation. METHODS:A total of 28 patients were selected from Department of Otolaryngology Head and Neck,Affiliated Hospital of Qingdao Medical College from June 2002 to April 2008. They were accessed by age,time of rehabilitation training,usage of hearing aids,audiology and aural image before surgery,and NRT monitoring in cochlear implantation so as to observe auditory improvement. The clinical results were analyzed to detect the effect of various factors on auditory rehabilitation. RESULTS:Age,preoperative preparations and good audiology and aural image are important premises for a successful rehabilitation. Longer the time of using hearing aids and language rehabilitation are,and better results will get. NRT response during operation is also an important factor for good auditory rehabilitation. CONCLUSION:The influential factors of auditory rehabilitation after cochlear implant included the age,audition remains,usage of hearing aids,NRT response during operation,and the time after the cochlear implant operation.

Objective To observe the effects of fluoride on testicular cell cycle and cell apoptosis of male rats.Methods Thirty-two healthy male Wistar rats,weighting 150-180 g,were randomly divided into 4 groups by body weight using random number table,normal sodium (control),the low-dose,medium-dose and high-dose groups(100,200,300 mg· kg-1· d-1 NaF,respectively) by intragastric administration for 90 days,and bodyweight was observed daily.After the last intragastric administration,all rats were killed by cervical dislocation.The testicular cell cycle and cell apoptosis were measured by flow cytometry.Results After 30 days exposure,the difference of body weight between groups was statistically significant(F =3.884,P ＜ 0.05).The body weights in low-and medium-dose groups[(235.00 ± 14.56),(235.44 ± 24.99)g] were significantly increased than that of high-dose group [(206.00 ± 18.16)g,all P ＜ 0.05].There was no significant difference of body weight between the groups at 0,60 and 90 days(F =0.501,0.578,1.893,all P ＞ 0.05).Compared with the control group[(43.10 ± 3.62)％],the percentages of G0/G1 stage cells were significantly increased in all the NaF-treated groups [(57.60 ± 7.26) ％,(52.80 ± 3.20) ％,(73.13 ± 4.08) ％] and the percentages of S stage cells were significantly decreased in all the NaF-treated groups [(10.58 ± 2.58)％,(9.35 ± 0.35)％,(9.55 ± 0.50)％] compared to the control group[(19.23 ± 0.61)％,all P ＜ 0.05].On the other hand,the percentage of G2/M stage cells decreased significantly in high-dose group[(17.18 ± 2.21)％] compared with the control group[(36.34 ± 5.05)％,P ＜ 0.05].The testicular cell apoptosis ratios in all the NaF-treated groups were higher than that in the control group,but only in medium-and high-dose groups[(71.03 ± 2.30)％,(71.90 ± 2.16)％],the difference was statistically significant compared with the control group [(60.80 ± 2.34)％,all P ＜ 0.05].Conclusion Chronic fluorosis can change testicular cell cycle and cell apoptosis and damage the reproductive system.

AIM To study the effects of parathyroid hormone(PTH) on intracellular calcium concentration([Ca 2+ ] i), hypertrophy and apoptosis in rat cardiomyocytes. METHODS Neonatal rat cardiomyocyte hypertrophic response was assayed by measuring cell surface area and protein content. [Ca 2+ ] i was measured by confocal microscope using Fluo 3/AM as fluorescent indicator.Apoptosis was determined by flow cytometer and electron microscope. RESULTS After 7 d exposure to PTH 1～34 of 0 01 and 0 1 ?mol?L -1 ,[Ca 2+ ] i,surface area and protein content,and apoptosis rate(APO) of cardiomyocytes were significantly increased,especially in high concentration groups. While application of both nifedipine and PTH 1～34 significantly decreased lose indicators, they were still markedly higher than normal. CONCLUSIONS PTH 1～34 dose dependently induces hypertrophy and apoptosis of cardiomyocyte,and this is attributed able to augmented entry of calcium into cell via voltage dependent Ca 2+ channel.

Objective To investigate the expression ofpodocalyxin(PCX) in patients with diabetic nephropathy (DN) and its clinical significance.Methods A total of 68 patients with DN were enrolled as DN group.Another 20 healthy person were selected as control group.The patients with DN were divided into three sub-groups:normal albuminuria group(＜ 30 mg/g,group A),microalbuminuria group(≥30 mg/g but ＜ 300 mg/g,group B) and macroalbuminuria group (≥ 300 mg/g,group C).The level of urinary PCX was measured with enzyme-linked immunosorbent assay.Results The level of urinary PCX,serum creatinine and blood urea nitrogen in DN group was higher than that in control group [(27.44 + 6.87) μ g/L vs.(3.52 +1.13) μ g/L,(154.82 ± 11.54) μ mol/L vs.(82.56 ± 5.71) μ mol/L,(8.79 ± 0.58) mmol/L vs.(4.23 ± 0.34)mmol/L,P ＜0.01].The level of urinary PCX in group A,B,C was (18.04 ±4.12),(23.58 ±5.43),(32.18 ± 6.05) μ g/L,and there was significant difference among three groups (P ＜ 0.05).The level of serum creatinine and blood urea nitrogen was (138.54 + 8.66),(142.16 ± 11.74),(165.13 ± 12.85) μ mol/L and (8.48 ± 0.43),(8.56 ± 0.61),(10.04 + 1.17) mmol/L in group A,B,C,there was no significant difference between group A and group B(P＞ 0.05),but there was significant difference between group A and group C,or between group B and group C (P ＜ 0.05).Conclusion The high-level expression of urinary PCX is found in DN patients,which maybe play a key role in the early diagnosis and assessment of the progression of DN.

Recently, the incidence of both diabetes mellitus and diabetic foot has been increasing.Ischemia, neuropathy, and infection are major causes of diabetic foot ulcer. In addition to other conventional treatments, hyperbaric oxygen is an effective adjunctive therapy with less side-effects. Hyperbaric oxygen may play a role through different mechanisms in improving the prognosis of diabetic foot.

Objective To observe the application of modified perfusion device pre-flushing method in double plasma molecular adsorption system (DPMAS) treatment and nursing care effect on patients with liver failure. Methods A retrospective analysis was conducted; 56 patients with liver failure who were consistent with the enrolled standard and admitted to the Surgical Intensive Care Unit (SICU) of Beijing Chaoyang Hospital fromJune 2014 to December 2016 were the objects of the study and their clinical data were collected. Ten patients involving the results of 17 case times from June 2014 to April 2015 were selected as the control group by using the traditional method of pre-flushing, and 46 patients involving the results of 68 case times from May 2015 to December 2016 were chosen as the observation group by using the modified perfusion device pre-flushing method. Both groups adopted effective nursing care cooperation: such as closely observe the changes of symptoms and signs of patients during the peri-treatment period, strengthen psychological care, maintain pipeline properly, and carry out the preventive management of anticoagulation and potential complications. The changes of symptoms and signs in the patients of two groups were observed, the DPMAS pre-flushing time and single time effective treatment time of the two groups were compared, before and 3 days after DPMAS treatment, the changes of serum total bilirubin (TBil), total bile acid (TBA) and its clearance rate, the changes of electrolytes, liver and kidney functions, blood routine and blood coagulation function were observed and compared between the two groups to evaluate and analyze the therapeutic effect of DPMAS.Results In the two groups, there were 56 patients involving 85 case times of DPMAS treatment all successfully completed, and the patients' symptoms and signs were improved significantly. The pre-flushing time of the observation group was obviously shorter than that in the control group (minutes: 29.5±13.1 vs. 38.9±14.7), and the single effective treatment time was obviously longer than that in the control group (minutes: 6.7±1.1 vs. 3.4±0.9,P < 0.05). After treatment, the TBil, TBA, alanine aminotransferase (ALT), aspartate aminotransferase (AST) and albumin (ALB) in two groups were decreased significantly compared with those before treatment, the prothrombin time (PT) was markedly prolonged compared with that before treatment (allP < 0.05), and the degrees of improvement in the observation group were more obvious than those of the control group (butP > 0.05), and urea nitrogen (BUN), creatinine (Cr), hemoglobin (Hb), platelet count (PLT), Na+ and K+ had no significant changes compared with those before treatment (allP > 0.05). The TBil clearance rate [(42.5±15.5)% vs. (32.9±13)%] and TBA clearance rate [(27±8.9)% vs. (17.1±5.8)%] in the observation group were significantly higher than those in the control group (allP < 0.05). There were no adverse events such as electrolyte disturbance, errhysis or bleeding found in the two groups during the treatment. In the study, there were 8 case times with self feeling of skin itching, 8 case times of skin rash, 6 case times of nausea and vomiting, 6 case times of chest tightness, 5 case times of blood pressure dropping phenomena and 4 case times of fever symptoms, and after the symptomatic treatments and nursing intervention, all the above symptoms were relieved or disappeared.Conclusion The modified perfusion device pre-flushing method can effectively elevate the pre-flushing effect and therapeutic effect, it is simple, time-saving, can reduce the economic burden of the patients, thus it is worthy to be used widely in clinic, during the therapeutic process, reasonable and effective nursing measures are practiced, that is the key to guarantee the successful treatment of patients.

Objective To explore the effect of Paclitaxel-Chitosan Sustain film on growth, apoptosis and cell cycle of biliary fibroblasts cells. Methods Human biliary fibroblasts cells were cultured and treated with PTX-CSF and naked PTX,separately, untreated cells as blank control. The experiment was divided into five groups:untreated group, simple PTX-treated group (250nM) and low, medium and high chitosan sustained-release film PTX-treated group (100 nM, 250 nM, 500 nM). The proliferations of cells were determined by MTT assay. The apoptosis and cell cycle of cells were detected by FCM. Results The proliferation of biliary fibroblasts cells was inhibited by PTX-CSF with time-dependent and dose-dependent, and the inhibiting effect was more obvious than naked PTX treatment as the time went on. Meanwhile, PTX-CSF could inhibit the magration of bile duct fibroblasts induced by TGF-β1,and had longer effect than naked PTX. After 72 h, the apoptosis rate of cells treated with PTX-CSF was significantly higher than cells treated with naked PTX or untreated cells(P<0.05), the difference between naked PTX or untreated cells was not significant. Compared with untreated cells, the proportion of G 2/M in cells treated with PTX or PTX-CSF were significantly increased, and the former was sinificantly higher than the latter(P<0.05). Conclusion Compared with naked PTX, PTX-CSF has strong cytotoxic effects and obviously sustained-release effect. The effective concentration can be maintain for a long time by PTX-CSF, and it could be as the novel drug delivery system to continuously inhibit proliferation of bile duct fibroblasts.