Objective To observe the effects of fluoride on testicular cell cycle and cell apoptosis of male rats.Methods Thirty-two healthy male Wistar rats,weighting 150-180 g,were randomly divided into 4 groups by body weight using random number table,normal sodium (control),the low-dose,medium-dose and high-dose groups(100,200,300 mg· kg-1· d-1 NaF,respectively) by intragastric administration for 90 days,and bodyweight was observed daily.After the last intragastric administration,all rats were killed by cervical dislocation.The testicular cell cycle and cell apoptosis were measured by flow cytometry.Results After 30 days exposure,the difference of body weight between groups was statistically significant(F =3.884,P ＜ 0.05).The body weights in low-and medium-dose groups[(235.00 ± 14.56),(235.44 ± 24.99)g] were significantly increased than that of high-dose group [(206.00 ± 18.16)g,all P ＜ 0.05].There was no significant difference of body weight between the groups at 0,60 and 90 days(F =0.501,0.578,1.893,all P ＞ 0.05).Compared with the control group[(43.10 ± 3.62)％],the percentages of G0/G1 stage cells were significantly increased in all the NaF-treated groups [(57.60 ± 7.26) ％,(52.80 ± 3.20) ％,(73.13 ± 4.08) ％] and the percentages of S stage cells were significantly decreased in all the NaF-treated groups [(10.58 ± 2.58)％,(9.35 ± 0.35)％,(9.55 ± 0.50)％] compared to the control group[(19.23 ± 0.61)％,all P ＜ 0.05].On the other hand,the percentage of G2/M stage cells decreased significantly in high-dose group[(17.18 ± 2.21)％] compared with the control group[(36.34 ± 5.05)％,P ＜ 0.05].The testicular cell apoptosis ratios in all the NaF-treated groups were higher than that in the control group,but only in medium-and high-dose groups[(71.03 ± 2.30)％,(71.90 ± 2.16)％],the difference was statistically significant compared with the control group [(60.80 ± 2.34)％,all P ＜ 0.05].Conclusion Chronic fluorosis can change testicular cell cycle and cell apoptosis and damage the reproductive system.

B7-H3,a newly discovered co-stimulatory regulatory protein member of the B7 family.Its mRNA is ubiquitously expressed in a wide spectrum of tissues.As a co-stimulatory or co-inhibitory signal molecule which can regulate immune response,B7-H3 plays an important role in the immune system.Besides,B7-H3 can be also involved in cancer progression via non-immunological.Recently,the aberrant expression of B7-H3 has been described in various malignancies,and significantly correlated with poor prognosis and cancer progression.Therefore,B7-H3 is considered as an early diagnostic and prognostic marker and therapeutic target for tumors,but the specific molecular mechanisms of B7-H3 regulation are poorly understood.The immune and gene therapy of tumor by target B7-H3 has made some progress.

BACKGROUND:Transfusion of bone marrow mesenchymal stem cells may become a novel and effective biological therapy for inflammatory bowel disease in clinical practice. Nevertheless, the oncological safety of the treatment is worrisome, and is a key to determine whether mesenchymal stem cells can be widely used in treatment of inflammatory bowel disease, and deserves further investigation. OBJECTIVE:To evaluate the therapeutic effect of bone marrow mesenchymal stem celltransfusion against inflammatory bowel disease in mouse models, and to clarify the effects of mesenchymal stem cells on tumorigenesis of inflammatory bowel disease. METHODS:Mouse model of colitis was established using Balb/c (H-2d) mice exposed to dextran sulfate sodium. Syngeneic bone marrow mesenchymal stem cells were transfused into mouse model through caudal vein. The therapeutic effect of mesenchymal stem cells was compared and observed, and pathological remission of colitis was evaluated. Mouse model of colitis-driven colon carcinogenesis was established using Balb/c (H-2d) mice exposed to dextran sulfate sodium and azoxymethane. Tumor formation within the murine colon was compared and observed after transfusion of mesenchymal stem cells. RESULTS AND CONCLUSION:In models of dextran sulfate sodium-induced colitis, weight loss and fecal occult blood were lessened in the bone marrow mesenchymal stem cellgroup compared with the phosphate buffered saline group. Histological damage score of colitis was less in the bone marrow mesenchymal stem cellgroup:mucosal structure of distal colon was almost intact under microscope, and there was smal area of epithelial defects and cryptal defects. Inflammatory cellinfiltration, proliferation of capil ary and smal vessels could be observed in mucosa and submucosa. Homing and colonization of mesenchymal stem cells in submucosa of inflamed colon could also be observed by in vivo tracing. In the dextran sulfate sodium/azoxymethane model of colitis-driven colon carcinogenesis, the number of intestinal tumors and tumor load were obviously less in the bone marrow mesenchymal stem cellgroup than in the control group. Results indicated that transfusion of bone marrow mesenchymal stem cells can apparently improve colitis lesions of mice with inflammatory bowel disease and inhibit carcinogenesis of colitis, which may provide theoretical support for the biological safety of mesenchymal stem cells transplantation for inflammatory bowel disease.

Objective To study and establish the extraction technology of hypoglycemic components of total flavonoids and polysaccharides from Gynura divaricata (L .) DC ..Methods Based on the singer factor test ,the influences of extracting times ,diameter of drug powder ,soaking time ,solid-liquid ratio ,ethanol concentration ,and extracting time on extraction ratio of total flavonoids were studied .The best extraction technology of total flavonoids from Gynura divaricata (L .) DC .was es-tablished through L9 (3)4 orthogonal tests .On the base of previous studies combine with L9 (3)4 orthogonal tests ,the best ex-traction technology of total polysaccharides from Gynura divaricata (L .) DC .was established .Results The optimized extrac-tion technology of total flavonoids was confirmed as follows :drug powder of less than 10 mesh diameter were soaking 0 .5 hour with 80% ethanol ,and solid-liquid ratio 1∶20 ,and extracting twice ,each time one hour .The optimized extraction technology of total polysaccharides was confirmed as follows :add 20 times water to the residue of ethanol ,extraction temperature 90℃ , and extracting twice ,each time one hour .The extraction rate of both total flavonoids and polysaccharides were more than 80% .Conclusion The extraction technology of hypoglycemic components of total flavonoids and polysaccharides from Gynura divaricata (L .) DC .established in this study is stable ,reproducible ,and lays a foundation for the further studies on hypogly-cemic components in Gynura divaricata (L .) DC .

OBJECTIVETo study the purification effect of macroporous resin combined with ZTC natural clarifying agent on Folium Gynurae Divaricatae extracts and to determine the ideal purification process.

METHODThe content of total flavonoids in Folium Gynurae Divaricatae was determined by ultraviolet spectrophotometry spectrophotometer. The reservation ratio and transferring ratio of total flavonoids were used as indicator to detect such impacting factors as the macroporous resin model, the pH value of ethanol-extraction liquid and elution agent as well as cleaning agents, the concentration of ethanol-extraction liquid, the flow speed for adsorption, the ratio of polyamide to crude drug, the ratio of diameter to height of polyamide column, the flow speed of cleaning agents, the alcohol content and flow speed of the elution agent.

RESULTThe optimized purification conditions of total flavonoids in Folium Gynurae Divaricatae were as follows: the macroporous resin model was HPD600, the pH value of ethanol-extraction liquid and elution agent as well as cleaning agents was adjusted to 6.0, the concentration of extraction liquid was 0.25 g x mL(-1), the flow speed for adsorption was 2 BV x h(-1), the ratio of crude drug and the resin was 4:1, the ratio of diameter to height of resin column was 1:10, the flow speed of cleaning agents was 5 BV x h(-1), the dosage of cleaning agents was 5 BV, the dosage of elution agent was 9 BV, with 70% alcohol as elution agent, and the flow speed of elution agent as 5 BV x h(-1). Under the purification condition, the content of total flavonoids increased from 2.47% to 24.2%.

CONCLUSIONMacroporous resin and ZTC natural clarifying agent used in combination can improve the internal quality of the product, shorten the production cycle, reduce use and cost of organic solvent, thus it is worth popularizing.

Asteraceae ; chemistry ; Flavonoids ; chemistry ; isolation & purification ; Resins, Synthetic ; chemistry ; Spectrophotometry, Ultraviolet

Objective To investigate the current staffing of nurses and need of hospital nursing human resources in China.Methods Data were collected from 181 secondary and tertiary hospitals and 9774 nurses in mainland China by questionnaires.Results The average doctor-nurse ratio was 1 ∶ 1.39 in tertiary hospitals and 1 ∶ 1.31 in secondary hospitals.The doctor-nurse ratios in 164 hospitals (97.6％) and the proportion of nurses in health care staff in 105 hospitals (61.8％) had not reached the standard set by the Ministry of Health of China.62.3％ nurses held secondary diploma for their initial nursing education.The constituent ratio of nurses held secondary diploma decreased,while the ratio of nurses held advanced diploma and bachelor degree increased in the last 5 years from 2003 to 2007.Sixty percent of newly employed nurses were contract nurses in 2003.The proportion increased to 78％ in 2007,and in some regions it accounted for more than 90％ of new nurses.The needs and constituent ratio of nurses with.secondary diploma and advanced diploma would decrease while nurses with bachelor degree and master degree would increase in the next 5 years (from 2009 to 2013).Conclusions The nursing shortage is still severe in China,and nursing staff mainly held secondary diploma for their initial nursing education.Most of new nurses were employed as conwact nurses.The need of hospitals for nurses has increased,especially for nurses with higher educational level such as bachelor degree and master degree,while the need for secondary diploma hold ers have decreased.The need for advanced diploma holders has increased in the last 5 years and would decrease in the next 5 years,but they would still be the majority of employed nurses.The authors suggested that the staffing of nursing manpower,the work environments and career development of contract nurses should be improved,and the initial nursing education should be upgraded to meet the needs of hospitals.

Objective To explore the effect of health education pathway on hospitalized children with unintentional injury and their caregivers. Methods From January to December 2015, a total of 100 children with unintentional injuries and their caregivers admitted to ICU in a class Ⅲ grade A hospital were selected as the research objects. They were divided into the observation group and the control group according to the random number table, with 50 cases in each group. Patients and their caregivers in the control group received routine health education, while patients and their caregivers in the observation group were given the pathway of health education based on the control group. The health education knowledge, hospitalization expenses, hospitalization days, rehospitalization rate and nursing satisfaction of children and caregivers in the two groups before and after intervention were compared. Results The degree of mastery of health education knowledge of children and the caregivers was 98% ,98% in the observation group and 86% ,80% in the control group, and the differences were statistically significant (χ2=4.89,9.55;P< 0.05). The hospitalization expenses, hospitalization days and rehospitalization rate of the observation group were lower than those of the control group, the differences were statistically significant (t=2.56,2.67,9.82; P< 0.05). The nursing satisfaction of the patients and the caregivers of the observation group was 94%, which was higher than 76% of the control group, the difference was statistically significant (χ2=15.78,P<0.05). Conclusions The pathway of health education can reduce the hospitalization expenses, rehospitalization rate and improve the nursing satisfaction of hospitalized children with unintentional injury, worthwhile for clinical use.

OBJECTIVETo confirm that the severity of inflammation can promote the colitis-associated colorectal cancer(CAC) and explore the function of STAT3 signal pathway in CAC.

METHODSMutagenic agent azoxymethane(AOM) and pro-inflammatory agent dextran sodium sulfate salt (DSS) were used to develop a mouse model of CAC. By changing the concentration of DSS (0, 1% and 2% respectively), the mouse model with different extent of severity of inflammation was developed and the risk of carcinogenesis among these groups was compared. The expression of STAT3 signal pathway was detected by immunohistochemistry staining.

RESULTSIn the evaluation of inflammatory severity, disease activity index, histopathological inflammation scores and the expression of pro-inflammation chemokines such as TNF-α, IL-6 and IL-12 in the higher inflammatory response group were higher than that in the lower inflammatory response group. The incidence of colorectal tumor was 100%(12/12) in the higher inflammatory response group and the incidence of colorectal tumor was 58.3%(7/12) in the lower inflammatory response group, and the difference between these two group was statistically significant (P<0.05). The multiplicity(number of tumors/colon) was 12.5±0.5 in the higher inflammatory response group and the multiplicity was 6.6±1.0 in the lower inflammatory response group, and the difference between these two groups was statistically significant (P<0.001). The tumor load(sum of tumor diameters per mouse) in the higher inflammatory response group was 44.2±2.4 mm and that in the lower inflammatory response group was only 18.7±2.7 mm, and the difference between these two groups was statistically significant (P<0.0001). Moreover, the expression of p-STAT3 (Tyr705) was higher in colitis tissue of the higher inflammatory response group than that of the lower inflammatory response group.

CONCLUSIONSInflammation can promote the colitis-associated CAC. And the activation of STAT3 signal pathway may promote the development of CAC.

Animals ; Azoxymethane ; Colitis ; complications ; Colonic Neoplasms ; Colorectal Neoplasms ; etiology ; pathology ; Dextran Sulfate ; Disease Models, Animal ; Immunohistochemistry ; Inflammation ; Interleukin-6 ; Mice ; Mice, Inbred C57BL ; STAT3 Transcription Factor ; Signal Transduction ; Tumor Necrosis Factor-alpha

Objective To explore the effects of continuous nursing on the quality of life and disease control in children with bronchial asthma .Methods 77 participants were randomly divided into the intervention group (n=39) and the control group (n=38).Based on the conventional nursing mode , the intervention group received additional continuous nursing after discharging from the hospital .The related nursing knowledge of the participants and their parents , children′s quality of life and their asthma control level were evaluated according to the continuing nursing assessment scale ( CTM ) , childhood asthma control test ( C.ACT ) and pediatric asthma quality of life questionnaire ( PAQLQ ) before the intervention and after six months of discharge . Results After six months of discharge , the scores of the nursing knowledge , quality of life and asthma control level of the participants in the intervention group significantly improved compared to the scores for the control group (P<0.01).The average scores for the nursing knowledge of the patients and their parents increased from (51.92 ±3.3) to (71.10 ±2.026) after six months of discharge (t=4.731,P<0.001).The scores for each dimension of the patients′quality of life in the intervention group were (6.48 ±0.49),(6.13 ±0.68),(6.23 ± 0.52), which were higher than the scores of (6.07 ±0.78),(5.77 ±0.67),(5.93 ±0.60) in the control group (t=2.816,2.352,2.320;P<0.05).The asthma control rate was 94.9% for the intervention group, which was higher than the rate of 68 .4%for the control group and the total rates of the asthma control also had significant differences between two groups (χ2 =9.052,P<0.05).Conclusions The continuous nursing mode is an effective method of discharge management that can significantly improve patients ′knowledge and awareness of asthma, as well as their quality of life and asthma control level .