At present, the prevalence of heart failure (HF) is still high in China, and its mortality and readmission rate have no downward trend. Therefore, the mechanism of its occurrence and development needs to be explored. In recent years, it has been found that about 13%-15% of HF patients have subclinical hypothyroidism (SCH), which can lead to cardiac systolic and diastolic dysfunction, thus promoting the occurrence and development of HF. At present, the curative effect of patients with mild elevation of thyroid stimulating hormone (TSH) level in HF patients with SCH is not clear, and a large number of prospective studies are still needed. In addition, in the complications of HF, SCH is the only disease that can reverse cardiac function after treatment. Therefore, this paper summarizes the mechanism of SCH in the occurrence and development of HF, and provides the basis for the formulation of clinical HF treatment strategies and the evaluation of prognosis.

Objective To analyze the characteristic of twenty-item premature behavioral neurological assessment,and to assess the risk factors of neonatal behavioral neurological assessment(NBNA) in premature infants. Methods Total two hundred and seventy-four premature infants hospitalized in Guangzhou Children's Hospital were assessed with the NBNA after correcting age 40 weeks,premature infants were divided into two groups:normal group (n =221 ) and abnormal group (n =53) according to NBNA. The birth history, mother pregnancy situation, the disease during neonatal period and results in hospital were reviewed,and the risk factors of NBNA in premature infants were assessed. Results There were significant differences in gestational age, birth weight, head circumference, body height, Apgar score, resuscitation time, hospitalization time and total gastrointestinal nutrition time between the two groups( P ＜ 0. 05 ). The incidences of superfoetation,hyaline membrane disease, hemorrhagic and hypoxic brain injury, severe infection, hyperbilirubinemia, hypoglycemia and chronic lung disease were different between the two groups( P ＜ 0. 05 ). There were no differences in gender, antenatal hormone use, anaemia, hypotension and evoked potential abnormality (P ＞0. 05 ). Multivariate logistic regression analysis showed that the risk factors of NBNA score in premature infants would include low Apgar score, low birth weight, hyaline membrane disease, hemorrhagic and hypoxic brain injury, severe infection, hyperbilirubinemia, hypoglycemia and chronic lung disease. Conclusion Although the premature infants corrected age 40 weeks,but the capability and initiative muscular tension are still lower than term infant. Low Apgar score, low birth weight, hyaline membrane disease, hemorrhagic and hypoxic brain injury ,severe infection ,hyperbilirubinemia,hypoglycemia and chronic lung disease are important risk factors related to premature behavioral neurological assessment.

Objective This study was to explore the inhibitory effect of shRNA-VEGF - C on growth of human colon cancer cell line Lovo in vitro and vivo. Methods Recombinant VEGF-C short hairpin RNA (shRNA) plasmid was constructed and transfected into Lovo cells. The expression of VEGF-C was detected at mRNA and protein levels by real-time reverse transcription-polymerase chain reaction (RT-PCR). In vivo study, xenograft tumors were established by injecting LOVO cells into nude mice, then shR-NA-VEGF-C were injected into the tumors, the tumor volume and weight and the incidences of lymph node metastasis were detected. Immunohistochemical staining was used to detect the lymphatic microvessel density of colon cancer tissues. Results After transfection of shRNA-VEGF-C, the mRNA of VEGF-C in Lovo cells were down-regulated. Four weeks after injection, the tumor volume and tumor weight in VEGF-C-shR-NA group were significantly smaller than that in empty plasmid group and NS group [(324. 9 ± 64. 8 ) mm3 vs. (553.5±90. 1)mm3 and (570. 1±85.4)mm3; (3.01 ±0.55)g vs (4.65 ±0.65)g and (4.75 ±0. 75)g]. The incidences of lymph node metastasis (30. 1% ) were significantly inhibited compared with empty plasmid group (50. 2% ) and normal saline group (53. 1% ). In shRNA-VEGF-C group, and microlymphatic density (15.5 ± 6. 90) was also decreased compared with empty plasmid group (24. 18 ±6. 45 ), and normal saline group (29. 59 ± 8. 21 ) ( all P <0. 01 ). Conclusion shRNA-VEGF-C can inhibit the growth of LOVO cells in vitro and vivo. VEGF-C may inhibit the lymph node metastasis of colon cancer by suppressing lymphangiogenesis.

The clinical application of GlucoWatch used in 32 Chinese diabetic patients was evaluated to determine its value in following blood glucose trends and track patterns and to know the compliance of patient. The results confirmed the accuracy of GlucoWatch and showed that it could follow blood glucose trends and track patterns. The skin irritation was mild and most subjects thought that blood glucose monitoring by GlucoWatch could reduce the pain caused by traditional methods.

AIM:To establish an effective and convenient method for applying HPLC fingerprints to quality control in the production of Shanzhuang Jiangzhi Tablet(Semen cassiae,Fructus crataegi,and Folium nelumbinis).METHODS:Diamonsil~(TM) C_(18)(150 mm×4.6 mm,5 μm)analytical column was used and eluted with a gradient program consisted of phase B(methanol)and phase D(1 % phosphoric acid)and detected at 254 nm;the column temperature was 30℃.The flow rate was 1.0 mL/min.RESULTS:Ten batches of sample tablets were tested and gained HPLC fingerprint of the tablet containing 17 common peaks.CONCLUSION:This validated method is available for quality evaluation and quality control in Shanzhuang Jiangzhi Tablet.

AIM:To establish a method for determining menthol, methyl salicylate,cinnamal dehyde and engenol simultaneously in Baicao Oil by GC. METHODS: A separation was performed on a large bore capillary column(30m?0.53mm) with PEG-20M as the stationary phase of GC.The method was carried out with FID as the detector without split stream sampling.A programmed temperature was employed.Benzyl alcohol was selected as the internal standard substance. RESULTS: An excellent seperation of menthol,methyl salicylate,cinnamal and engenol in Baicao Oil was gained.The good linear relationships of menthol (62.7?10~3-2.01?10~3 ?g/mL),methyl salicylate (119?10~3-3.81?10~3 ?g/mL),cinnamal dehyde (0.42-205 ?g/mL),engenol (19.9-636 ?g/mL) were observed.All correlation coefficients were greater than (0.999 2).The average recovery of menthol,methyl salicylate,cinnamal and engenol were between(98.6%)～100.3%,and RSD was less than 2.4%. CONCLUSION: The method is simple,accurate and suitable for quality control of Baicao Oil.

AIM:To establish an effective and convenient method for applying HPLC fingerprints to quality control in the production of Shanzhuang Jiangzhi Tablet(Semen cassiae,Fructus crataegi,and Folium nelumbinis).METHODS:DiamonsilTM C18(150 mm?4.6 mm,5 ?m) analytical column was used and eluted with a gradient program consisted of phase B(methanol) and phase D(1% phosphoric acid) and detected at 254 nm;the column temperature was 30 ℃.The flow rate was 1.0 mL/min.RESULTS:Ten batches of sample tablets were tested and gained HPLC fingerprint of the tablet containing 17 common peaks.CONCLUSION:This validated method is available for quality evaluation and quality control in Shanzhuang Jiangzhi Tablet.

Objective The purpose of this study was to determine the value of K-ras mutation in DNA extracted from the plasma as clinical indicator of colorectal cancer. Methods Point mutation at codon 12 of K-ras gene was assayed by polymerase chain reaction of sequence-specific primers in DNA extracted from the plasma and tumors from 32 patients with colorectal cancer. The mutation was further confirmed by dideoxy-mediated chain-termination method of DNA sequencing. Results Fourteen patients (44%) had a codon 12 K-ras mutation within their primary tumors and identical mutations were found in the plasma DNA of 13 patients (93%). Mutant DNA was not detected in the plasma specimens of 18 patients whose tumors tested negative for K-ras alterations or in 5 healthy control subjects. Conclusion Preliminary results suggest the detection of K-ras mutations in circulating DNA extracted from the plasma specimens may have some clinical uti- lity in the detection of colorectal cancer.

Objective To study changes of telomerase activity in transitional cell carcinoma after intravesical instillation of Serratia Marcescens . Methods TRAP-PCR and TRAP-sliver staining were used to detect telomerase activity in 20 patients with TCC after intravesical instillation of Serratia Marcescens . Results Positive rate of telomerase activity detection in TCC patients after intravesical instillation of Serratia Marcescens was 50%(10/20),significantly lowed than before the intravesical instillation (90%,18/20), P

The fibroblasts have long been considered as structural elements and playing a main role in wound healing. Recent studies indicated that fibroblasts are heterogenic cells and function as resident sentinel cells in inflammatory reaction. When activated by substances released during tissue injury or derived from infectious microorganisms or by proinflammatory cytokines, tissuespecific fibroblasts can transiently produce certain chemokines and initiate a cascade of inflammatory reaction. In addition fibroblasts can produce prostanoids that participate in inflammatory and immune responses. In this review, the new role of fibroblasts in regulation of inflammation and immune reactions and possible molecular mechanism will be discuss.