Objective To investigate the family care needs in patients with primary hepatocellular carcinoma after transcatheter hepatic arterial chemoembolization (TACE).Methods One hundred and twenty patients of hepatocellular carcinoma with non first time of TACE were selected from August 2013 to June 2014 by convenience sampling method.Using a self designed questionnaire form,investigated patients' families care after discharge needs,including psychological demand,nursing requirements and other requirements.The independent factors affecting families psychological needs were analyzed by multi-factor analysis of variance.Results Patients' families care needs in dimensions,psychological demand was the highest score for (3.12±0.81) scores.Care requirements and other requirements were (3.04±0.56),(2.18±0.36) scores,respectively.Each demand entries,understand pain processing requirements was the highest score for (3.78 ±1.02) scores,hospice and funeral support was the lowest for (1.27±0.38) scores.Education degree,and patient relationship were independent factors affecting patients' families psychological demand,P＜0.05.Conclusions TACE reduces quality of life of patients,the role of families in the care of patients is also important.Care needs of families after TACE,the psychological demand should be satisfied first,required at the same time attention to relevant nursing requirements and other requirements;while education from families and relationships with patients are independent factors affecting the psychological needs of patients' family members.

Objective:To investigation for common hepatic cartery lymph node metastasis of esophageal squamous cell carcinoma.Methods:From 2015 to 2017, 413 patients with esophageal squamous cell carcinoma who were dissected the common hepatic cartery lymp node were admitted in Cancer Hospital of Sichuan. The relationship between metastatic of rates of common hepatic cartery lymp node and the clinical data including sex, age, tumor site, diameter of tumor, invasion depth, differentiation degree, pathological stage, neurovascular invasion status were reviewed retrospectively.Results:A total of 10 649 lymph nodes were dissected.The lymph node metastatic rate was 45.27%(187/413), and the metastatic lymph node ratio was 7.96%(848/10 649). Lymph nodes adjacent to the middle esophagus, cervical esophagus, cardia of stomach had a higher metastatic rate, while the Lymph nodes adjacent to the hilar, supraclavicular, common hepatic cartery had a lower metastatic rate.A total of 775 lymph nodes were dissected in common hepatic cartery.The lymph node metastatic rate was 7.02%(29/413), and the metastatic lymph node ratio was 4.51%(35/775). To analysis the relationship between metastatic of rates of common hepatic cartery lymp node and the clinical data.We conclusion that the common hepatic artery lymph node metastatic rates of upper, middle and lower esophageal squamous cell carcinoma were 2.89%(2/69), 5.35%(12/224) and 12.50%(15/120), with significant difference.The common hepatic artery lymph node metastatic rates of patients with diameter of tumor under 3 cm, 3-5 cm and above 5 cm were 5.20%(9/173), 5.52%(9/163)and 14.28%(11/77), with significant difference.The common hepatic artery lymph node metastatic rates of sex, age, tumor site, diameter of tumor, invasion depth, differentiation degree, pathological stage, neurovascular invasion status, with no significant difference.Conclusion:The metastatic rates of common hepatic artery lymph node in esophageal squamous cell carcinoma is lower. For the upper thoracic esophageal cancer with tumor diameter under 5 cm, the dissection of common hepatic lymph node can be ommitted in surgery.

Objective To summarize our experience in management of infected femoral artery pseudoaneurysm caused by addictive drugs injection.Methods Clinical data of 45 cases of infeted femoral artery pseudoaneursysm were reviewed retrospectively.When infected femoral artery pseudoaneurysm was diagnosed,the femoral artery or external iliac artery was ligated and the pseudoaneurysm was resected in all of cases.Anticoagulants and antibiotics were used after operation.Results Lower limbs were salvaged in 44 patients.The ambulation function of affected limb was fine in the short-term.One limb was amputated because of lower limb deep venous thrombosis and gangrene.Thirty-four patients(75.6%) were followed up for 3-12 months,the results were satisfactory.Conclusions Femoral artery or external iliac artery ligation is an effective procedure for management of infected femoral artery pseudoaneurysm.

AIM:To establish an effective and convenient method for applying HPLC fingerprints to quality control in the production of Shanzhuang Jiangzhi Tablet(Semen cassiae,Fructus crataegi,and Folium nelumbinis).METHODS:Diamonsil~(TM) C_(18)(150 mm×4.6 mm,5 μm)analytical column was used and eluted with a gradient program consisted of phase B(methanol)and phase D(1 % phosphoric acid)and detected at 254 nm;the column temperature was 30℃.The flow rate was 1.0 mL/min.RESULTS:Ten batches of sample tablets were tested and gained HPLC fingerprint of the tablet containing 17 common peaks.CONCLUSION:This validated method is available for quality evaluation and quality control in Shanzhuang Jiangzhi Tablet.

AIM:To optimize the technical parameters indica-daisy dropping pill(Flos chrysanthemi indici) through controlling the influencing factors. METHODS:To take weight variation of pills,comprehensive quality and disintegration time limited as index,the above factors were observed by orthogonal test. RESULTS:Good technological parameters of indica-daisy dropping pill were as follows PEG6000∶PEG400=54∶6 as matrix,dimethicone as refrigerant.Temperature of drug fluids of 70 ℃,internal and external diameter of dropper within 6.2 mm and 9.0 mm,proportion of drug and matrix(1∶3),30 dropping per minute.The weight variation of pills was small,type quality was good and disintegration time limited was short.The water absorbability was smaller than that of granules. CONCLUSION:The finished products are of good quality and appropriate for mass production.

AIM:To establish an effective and convenient method for applying HPLC fingerprints to quality control in the production of Shanzhuang Jiangzhi Tablet(Semen cassiae,Fructus crataegi,and Folium nelumbinis).METHODS:DiamonsilTM C18(150 mm?4.6 mm,5 ?m) analytical column was used and eluted with a gradient program consisted of phase B(methanol) and phase D(1% phosphoric acid) and detected at 254 nm;the column temperature was 30 ℃.The flow rate was 1.0 mL/min.RESULTS:Ten batches of sample tablets were tested and gained HPLC fingerprint of the tablet containing 17 common peaks.CONCLUSION:This validated method is available for quality evaluation and quality control in Shanzhuang Jiangzhi Tablet.

Objective To evaluate the impact of hydrogen sulfide signal system during the process of rat bone marrow-derived mesenchymal cells osteogenesis under tensile stress. Methods After the H2S signal system of cell rooms regulated by tool drugs (Group A with propargylglycine;Goup B with PBS;Group C with GYY3147), 4 000 μ strain tensile stress were applied on rat BMMCs by four-point bending apparatus for 60 minutes. Four hours later, the H2S signal system and cystathionine-γ-lyase were detected. Meanwhile, the change of the alkaline phosphate, osteocalcin, procollagen typeⅠN-terminal propeptide, and runt-related transcription factor 2 mRNA level were also examined to evaluate the osteogenic ability. Results With the increase of H2S expression, the osteogenic capacity gradually increased, while the osteogenic capacity was compromised after the endogenous cystathionine-γ-lyase was inhibited. Conclusion The H2S signal system plays an important role during BMMCs osteogensis under tensile stress. The up-regulation expression of H2S may promote osteogenesis during distraction osteogenesis.

Objective To compare the impact on the ovarian reserve function after cisplatin intraperitoneal or intravenous chemotherapy in rats model. Methods Thirty 8-weeks old female Sprague Dawley rats were randomly assigned to control group (group A, n=10), intraperitoneal chemotherapy group (group B, n=10) and intravenous chemotherapy group (group C, n=10). Cisplatin was diluted by normal saline (NS) into 4 mg/ml. On the first day of chemotherapy, 0.2 ml cisplatin dilution was injected into the abdomen of rats in group B, isodose cisplatin was injected into vein and 1.8 ml NS was injected into abdomen of rats in group C, 2.0 ml NS was injected into abdomen of rats in group A for control. Feed the three groups rats and test the anti-Mullerian hormone (AMH) in serum on day 0 (just before injection), day 10 and day 20 by ELISA, count the numble of follicle in bilateral ovaries on day 20. Results (1) The levels of serum AMH in the three groups before and after chemotherapy were compared: ① comparison between groups: On day 10 and day 20 , the AMH level in group B [(64.5 ± 2.9), (68.6 ± 3.4) ng/L] and group C [(76.1±4.9), (91.3±3.9) ng/L] was significantly lower than that in group A [(120.1±5.3), (121.7±4.6) ng/L;P<0.01], AMH level in group B was significantly also lower than that in group C (P=0.000). ② Comparison within groups:the AMH level on day 0 was significantly lower than that on day 10 and day 20 in group A (P<0.01), but there was no significant difference between day 10 and day 20 (P=0.427). The AMH level on day 0 was significantly higher than those on day 10 and day 20 in group B (P<0.01) and group C (P<0.01). There was no difference in AMH level between day 10 and day 20 (P=0.124) in group B, but the level was significant lower on day 10 than that on day 20 in group C (P=0.011). (2)Comparison of the number of follicles in ovaries of three groups 20 days after chemotherapy:the follicles number in group A(35±13)was greater than that in group B (16 ± 9,P=0.003) and similar with group C (31 ± 16,P=0.474) on day 20. The follicles number in group B was significantly less than that in group C (P=0.018). Conclusions In the present study, both intravenous and intraperitoneal chemotherapy have impacts on ovarian reserve function and the latter might be more serious. The level of AMH will rise again over time after chemotherapy and rats undergo intravenous chemotherapy would recover faster. The results suggest that the ovarian reserve function need more time to recover after intraperitoneal chemotherapy. For patients with ovarian cancer who want to preserve fertility function, intravenous chemotherapy might be more appropriate.

BACKGROUND: Hydrogen sulfide signaling has been proved to promote distraction osteogenesis; however,the underlying mechanism remains unclear.OBJECTIVE: To evaluate the relationship between hydrogen sulfide and hypoxia-inducible factor-1α (HIF-1α)during the osteogenesis of rat bone marrow mesenchymal cells under tensile stress.METHODS: 2000 μ strain was loaded on the in vitro cultured rat bone marrow mesenchymal cells by a four-point bending apparatus, and hydrogen sulfide donor or HIF-1α inhibitor was adopted in the tensile unit. Subsequently, the levels of osteogenic markers were detected.RESULTS AND CONCLUSION: Exogenous hydrogen sulfide signaling could promote the osteogenesis of rat bone marrow mesenchymal cells under tensile stress. However, this promotion was obviously eliminated when the endogenous HIF-1α expression was inhibited.These results show that hydrogen sulfide signaling system promotes the osteogenesis of rat bone marrow mesenchymal cells under tensile stress probably through HIF-1α.

BACKGROUND:Long course of treatment and complications limit the extensive application of distraction osteogenesis, which cannot meet the clinical requirements. OBJECTIVE:To investigate the effect of general administration of eucommia alcohol extract on new bone regeneration based on the rabbit model of mandibular distraction osteogenesis. METHODS:Twenty-four New Zealand white rabbits were randomly divided into experimental group and control group. Unilateral mandibular distraction osteogenesis model was established by 1 mm/12 h distraction protocol. During the distraction period, the rabbits in the experimental group were intragastricaly administered with eucommia alcohol extract and the control animals received the same amount of physiological saline, respectively. Six weeks later, the animals were sacrificed for osteogenic testing. RESULTS AND CONCLUSION: New bone formation was observed in the distracted gap in both groups. However, the amount, mineralization and biomechanical strength of new regenerated bone in the experimental group were obviously greater than those in the control group by histological observation, dual energy X-ray absorptiometry, micro-CT and biomechanical test. General administration of eucommia alcohol extract can markedly promote distraction osteogensis in rabbit mandibular osteodistraction.