Objective To explore the effective perioperative nursing measures for congenital cleft lip and palate children.Methods 43 cases of congenital cleft lip and palate children treated in our hospital from January 2010 to December 2011 were given perioperative nursing measures.Results The wound healing time was 6～7 days,with an average time of 6.6 d.After operation,a survey was carried out to investigate the satisfaction degree of the parents,the results showed that postoperative satisfaction degree of the parents reached 100％.Conclusions Nursing intervention is of great significance on the efficacy of cleft lip and palate surgery,the implementation of comprehensive nursing interventions can obtain desirable clinical effect,and increase the satisfaction degree of children's parents.

Objective To establish the quality standard of Heibu ointment. Method The contents of Galla Halepensis were determined by TLC, and Gallic Acid was determined by HPLC. Results In TLC, the spots were very clear and specific to identify Heibu ointment. The linear range of Gallic Acid was 0.1～1.1 ?g. The quality standard of Heibu ointment was established. Conclusion The method can be used for quality control of Heibu ointment.

Objective To seek optimum extraction conditions of Radix Scutellaria in Qinbai ointment by studying on extraction process. Method The optimum extraction regard baicalin as the index. It was investigated by the orthogonal design and determined by HPLC. Result The effect of alcohol concentration has extremely remarkable difference (P

It is an important approach for CAD/CAM of prosthetic sockets to establish digital model of residual lower limbs from amputees. In this thesis, ultrasonic imaging technology is utilized to extract the internal and external contours of residual lower limbs for their 3D modeling , and an ultrasound measurement system is developed.

OBJECTIVE: To study the curative efficacy of Shuxuetong injection for vascular dementia(VD).METHODS: A total of 60 patients with VD were randomized to receive Shuxuetong injection(6 mL) added into normal saline(250 mL)q.d by drip infusion for 21 days(treatment group) or citicoline injection(250 mg) added into 250 mL normal saline q.d by drip infusion for 21 days(control group).In addition,both groups received basic treatment and nursing care,and the antihypertensive,hypoglycemic and hypolipidemic therapies were adopted based on the patients' severity of disease and complications.The efficacy was evaluated before and after treatment by using mini-mental state examination(MMSE),activities of daily living(ADL),transcranial Doppler(TCD) and P300 test.RESULTS: Compared to control group,the treatment group had significantly higher MMSE score and ADL score(P

Objective To explore the histopathological changes of the radial optic neurotomy (RON) in human eyes, and to establish the theoretical foundation for the effective RON. Methods Ten patients with unaffected eyeball or optic disc who had undergone ophthalmectomy (7 patients) or orbital exenteration (3 patients) because of intraocular or orbital tumor were gathered. A double-incision in pars plana was performed. One was inserted into illuminating fiber, and another was inserted into a standard microvitreoretinal (MVR) blade (unbent MVR blade in 4 patients and bent ones in 6; radial incision on nasal side of the optic disc in 4 patients and on both nasal and temporal side in 6). The histopathological examination was performed to observe the location and depth of the incision. Results Eleven incisions were found in 8 out of 10 patients, of which surgical spaces can be observed clearly. Three incisions were obliquely inserted into the optic nerves, 5 sieve plate lateral incisions had the surgical intervals connected with the subarachnoid spaces, and 3 incisions caused obvious damage of retinal ganglion cell axons due to the position close to the center. Nine incisions approached to the retrolaminal level, and the other 2 reached the laminal and prelaminal level respectively. Injured retina beside the optic disc in 1 incision was found. Central retinal vessels were not damaged. Conclusions An optimal incision may be in the edge of the lamina cribrosa, whose depth should be in the level of lamina cribrosa and retrolamina, with surgical intervals connected with the subarachnoid spaces.

Objective To construct the eukaryotic expression vector with the mutant of hypoxia inducible factor 1α (HIF-1α) that could over express nonhypoxic dependent HIF-1α and investigate its expression in eukaryotic cells PC12. Methods The coding sequence of HIF-1α was amplified from total RNA from the injured spinal cord of rats by means of RT-PCR. Then, overlapping extension PCR was employed to obtain one complete sequence encoding HIF-1α mutant without oxygen-sensitive degradation domain (ODD). By using gene recombination technique, the gene HIF-1α△ODD was inserted into eukaryotic expression vector _pEGFPC1 that contained a reporter gene of enhanced green fluorescent protein. The recombinant plasmid was successfully constructed and transfected into the neural cell line PC12. The expression of HIF-1α△ODD was analyzed by fluorescent observation and Western blot. Results A 2kb gene fragment encoding H1F-1α△ODD was successfully obtained by overlapping extension PCR. On this basis, the recombinant plasmid _pEGFPC1-HIF-1 α△ODD was constructed and testified with restriction enzyme digestion and DNA sequencing. After this recombinant plasmid was transfected into the neural cell line PCI2, both fluorescent observation and Western blot demonstrated that HIF-1α△ODD was over expressed under nonhypoxia conditions. Conclusion The eukaryotic expression vector _pEGFPC1-HIF-1α△ODD is successfully constructed and HIF-1α△ODD over expresses in transfected PCI2 cell lines.

Osteoporosis, which is characterized by reduced bone mineral density (BMD) and an increased risk of fragility fractures, is the result of a complex interaction between environmental factors and genetic variants that confer susceptibility. Fracture and other complications caused by osteoporosis have serious impact on the life quality and life span of patients. Although previous linkage and candidate gene studies have provided few replicated loci for osteoporosis, genome-wide approaches and next generation sequencing have produced clear and reproducible findings. To date, 25 genome-wide studies for osteoporosis and related traits have been conducted, identifying 76 genes and loci. In this review, we will update the genetic study of osteoporosis and provide some perspective views.

It was studied that effects of Hg 2+ ,Cu 2+ ,Cd 2+ ,Cr(Ⅵ) and Pb 2+ on 96h average growth rate,conductivity of osmotic luquor and total protein and bacteriochlorophyll content of our Rhodopseudomonas palustris The results showed that the toxicity sequence of these heavy metal ions from high to low is Hg 2+ , Cu 2+ , Cd 2+ ,Cr(Ⅵ)and Pb 2+ according to the results of effects of 5 heavy metal ions on 96h average growth rate of this strain;forcing of Hg 2+ , Cd 2+ ,Cr(Ⅵ) and Pb 2+ lead to increasing of conductivity of osmotic luquor ,and this effect has dose response,but that of Cu 2+ doesn't ;forcing of 5 heavy metal ions could result in reducing of bacteriochlorophyll content,and this effect also has dose response But forcing of 5 heavy metal ions hasn't marked effect on total protein content

Objective To evaluate the efficacy of medial femoral tubed skin flap combined with penile elongation for repairing long-segment penile defects.Methods From June 2009 to January 2015,13 cases of long-segment penile defects were treated by medial femoral tubed skin flap with pe nile elongation.Results All cases were followed up for 0.5 to 5.0 years.All flaps survived and all patients had the minor scar in donor areas,with good appearance and feeling of penis.The length of pe nis in flaccid state was 6.3±1.6 cm.The length of penis in erection state was 8.5±1.2 cm;The circumference of penis was 5.3±1.9 cm.1.0 cm × 0.7 cm of necrosis at distal parts occurred in one case early after operation,and healed after dressing changes.Two cases had tumid appearance which influenced penile appearance and sexual intercourse.It was improved by local liposuction.No feeling and e rectile dysfunction developed during the following-up.Except 5 cases who did not married,the other married patients and their partners were satisfied or quite satisfied with sexual intercourse activity.Conclusions Medial femoral tubed skin flap combined with penile elongation is a good method for the treatment of long-segment penile defects.The prosthetic penis could have suitable length,good ap pearance,good feeling and erectile function.