In this paper, the function of the iel gene from baculovirus Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV), belonging to group Ⅱ nucleopolyhedrovirus, was studied in mammalian cells.We amplified the SeMNPV ie1 gene and expressed it by fusing to the C terminal of enhanced GFP protein in HEK 293 cells. Confocal microscopy revealed that the IE1-GFP fusion protein was localized in the nucleus of the mammalian cells. The promoter sequences of AcMNPV gp64, SeMNPV F protein and Drosophila hsp70 were also analyzed, to further study the function of SeMNPV IE1. The results showed that, in the absence of the hr sequence, IE1 improved the expression of the F promoter but didn't influence the gp64 promoter significantly, but IE1 moderately stimulated the hsp70 promoter.

Objective To investigate the value of MR multiparameter imaging for the clinical classification of chronic pancreatitis. Methods 65 patients with confirmed chronic pancreatitis by follow-up and pathologic examinations ( 14 mild, 37 moderate and 14 severe according to MANNHEIM system) and 20healthy volunteers were included in this study. MR examination including routine T1WI, T2WI, MRCP and dynamic enhanced MRI. The data were measured and statistical analysis was applied in four groups. Two radiologists assessed pancreatic duct diameter, pancreatic size, pancreatic cyst, pancreatic stone and pancreatic signal intensity on MRCP, T1-weighted and T2-weighted images. Pancreatic signal intensity were also measured on dynamic enhanced MR. Results Mean values of pancreatic signal intensity ratio on T1WI (rT1)in the pancreas were significantly reduced in patients with moderate and severe CP compared with volunteers.There was significant difference among four groups ( normal, 0. 98 ±0.27; mild, 0. 84 ±0. 12; moderate,0.81 ±0. 16; severe, 0.75 ±0.24). Mean values of pancreatic signal intensity ratio on T2WI (rT2) in the pancreas were no difference among four groups ( normal, 1.28 ± 0.3; mild, 1. 46 ± 0. 44, moderate, 1.46 ±0.55; severe, 1.76 ± 0.72). Pancreatic duct diameters were significantly increased in mild, moderate and severe CP groups [ mild (5.3 ± 2.4) mm; moderate ( 6.5 ± 3.3 ) mm; severe ( 8.1 ± 4.1 ) mm ] compared with patients without CP[ (2.0 ±0.6) mm; P < 0.01 ]. Severe degree of Cambridge classification was graded as mild in 4 (29%), moderate in 33 (89%), severe in 13 (93%). Pancreatic calcification was graded as mild in 2 ( 14% ), moderate in 11 (30%), severe in 5 (36%). Pancreatic pseudocyst was graded as mild in 0, moderate in 6 ( 16% ), severe in 3 (21.43%). Pancreatic parenchymal atrophy was graded as mild in 4 (29%), moderate in 22 (59%), severe in 10 (71%). They did not vary among CP groups. Parenchymal/arterial phase enhanced ratio (P/A) in the pancreas were significantly increased in patients with mild,moderate and severe CP (mild, 1. 10 ±0.08; moderate, 1.37 ±0.15; severe, 1.48 ±0.53) compared with patients without CP (0.88 ± 0.08, P < 0. 05 ). Significant correlation was present between the severity level of CP and the change of rT1, severe degree of Cambridge classification, the pancreatic duct diameter and P/A (r=0. 34, 0.41, 0. 62, - 0. 43; P < 0. 01 ). ROC analysis showed the presence of pancreatic duct diameters more than 2.5mm, rT1 less than 0. 8 and P/A more than 0. 8 had a sensitivity and specificity of diagnosing chronic pancreatitis of 94% and 79%, 90% and 48%, 95% and 47% respectively. Combined with the three variables, the specificity of diagnosing chronic pancreatitis can be improved to 95%.Conclusions T1-weighted, MRCP and dynamic enhanced MRI imaging can accurately evaluate the clinical severity of chronic pancreatitis. MRCP had the highest sensitivity and specificity, followed by T1-weighted and dynamic enhanced MRI imaging.

Objective To investigate the effects of magnetic iron-oxide (Fe3O4) nanoparticles with different skin materials on cytotoxicity against human cervix cancer HeLa cells.Methods The Fe3O4 nanoparticles were prepared by solvent-free dissolving method and then covered with poly(lactic-co-glycolic acid) (PLGA) and cholic acid (CA)-PLGA respectively.After the characterization,the uptake of nanoparticles by HeLa cells was observed by laser scanning confocal microscopy,and thiazolyl blue tetrazolium bromide (MTF) assay was used to assess the cytotoxcity of the Fe3O4-loaded nanoparticles against HeLa cells.Results The size of single Fe3O4 nanoparticle was about 7 nm.The Fe3O4-loaded PLGA and CA-PLGA nanoparticles were spherical in shape under transmission electron microscope,around 200 nm in diameter,and the theoretical drug-loading capacity was 10％.At the same Fe3O4 nanoparticles concentration of 25 μg/ml,the Fe3O4-loaded CA-PLGA nanoparticles showed less toxicity than Fe3O4-loaded PLGA nanoparticles.Conclusions Star-shaped copolymer CA-PLGA is promising in decreasing cytotoxicity of magnetic Fe3O4 nanoparticles and expanding its application in living organisms.

Objective A retrospective analysis of the situation of newborn disease screening in Longwan Wenzhou from 2002 to 2009 was conducted.Methods The restdts of newborn diaease screening of 46 202 neonatus in Longwan district of Wenzhou between 2002 and 2009 were analyzed.The neonatns were screened within 72 hours after birth for congenital hypothyroidism(CH),phenylketonuria(PKU)by measuring thyroid-simulating hormone (TSH)and phenylalanine concentration on dried blood spots on filter paper cards.Neonatus weYe recalled after screened positive and the diagnosis was made by testing serum concentration.Results A total of 46 202 newborns were screened from 2002 to 2009,and 23 cases was positive and the total positive rate was 0.05%,22 cases were confirmed positive for CH.with an incidenee of 1/2 100.1 case was confirmed positive for PKU,with an incidence of 1/46 202.All patients were followed-up by experts of newborn disease screening centre of Zhejiang Province.Conclusion The results of this study confirm that newborn discease sereaning was one of the effective methods of early diagnosis of congenital CH and PKU,which could effectively prevent development low of intelligence or physique,and other organs impairment.

Star-shaped block polymers have recently attracted considerable attention owing to their unique properties and applicability in nanomedicine development.Compare to linear polymer and hyper-branched polymers,star-shaped block polymers have more advantages due to the unique structure.Star-shaped block polymer has become a desirable molecular biomaterial for delivery therapeutics and diagnostic agent to the diseased cells.This paper reviews the research about star-shaped block polymers and nanomedicine development in using them as drug and gene delivery carriers.

Objective To understand the distribution and antimicrobial resistance of pathogens isolated from blood culture in a hospital in Sanya city.Methods Blood culture specimens and antimicrobial susceptibility testing results in this hospital from January 2013 to December 2015 were analyzed retrospectively.Results A total of 356 isolates of pathogenic bacteria were isolated from 3 195 blood culture specimens,the positive rate was 11.14％,including 215(60.39％)gram-negative bacterial strains,122(34.27％) gram positive bacterial strains,and 19(5.34％) fungi strains.The top 3 gram negative bacteria were Escherichia coli (n =90,25.28％),Klebsiella pneumoniae (n =60,16.85％),and Burkholderia pseudomallei (n =24,6.74％);the most common gram positive bacteria were Staphylococcus aureus (n =42,11.80 ％),coagulase-negative staphylococcus (n =38,10.67 ％),and Streptococcus spp.(n =33,9.27 ％).Resistance rates of Escherichia coli and Klebsiella pneumoniae to cefoperazone/sulbactam,amikacin,and carbapenems were all lower than 10.00％;resistance rates of Burkholderia pseudomallei to most antimicrobial agents were lower than 10.00％.There were no strains of main gram-positive bacteria that were found to be resistant to linezolid and vancomycin.Conclusion Gram-negative bacteria are the main pathogens causing bloodstream infection in this hospital in recent years,especially the isolation rate of Burkholderia pseudomallei is higher,which should arouse more attention in clinic.

Objective: This paper investigates Shenmai Injection’s effect on the cellular immunity of patients with acute exacerbated chronic obstructive pulmonary disease (AECOPD). Methods: 92 cases of AECOPD patients are randomly divided into two groups----control group and treatment group, each with 46 patients. Patients in the two groups are given the same treatment such as anti-inflammatory, astiasthmatic, eliminating phlegm, inhale oxygen. Based on conventional treatment, the treatment group dissolves 40mL of Shenmai Injection in 250mL of injection glucose (5%). Such solution is dripped in vein continuously for two courses of treatment, with ten drops for a course of treatment and once a drop. Indexes of CD+3, CD+4, CD+8of peripheral blood T lymphocyte subsets and ratio of CD+4/CD+8 are tested before two weeks and after two weeks of the treatment, in order to discuss the effect of Shenmai Injection on the cellular immunity of patients with acute exacerbation of chronic obstructive pulmonary disease. Results: After two weeks' treatment, the indexes of CD+3, CD+4 raise up, the indexes of CD+8 go down, and the ratio of CD4+/CD8+ increases. In contrast, there is a significant difference for the patients before the treatment; So is the same with Indexes of CD+3, CD4+, CD8+ and ratio of CD+4/CD+8 to the patients in the treatment group and those in the control group after two weeks. Conclusion: Shenmai Injection can improve the AECOPD patient’s cellular immunity.

Objective To investigate the diagnostic value of transbronchial needle aspiration (TBNA) and transbronchial biopsy(TBB) in central lung cancer.Methods Retrospectively analyzed the diagnostic data of 150 patients with central lung cancer who underwent TBNA followed by TBB in our hospital from January 2011 to October 2012.Results Among 150 patients with central lung cancer,33 cases had intracavity mass.Thirtythree cases (100％) were confirmed by TBB and 32 cases (97.0％) confirmed by TBNA.There was no statistically significant difference between the diagnosis of TBB and TBNA(P =0.90).There were 26 cases with simple stenosis,among which 1 case(3.9％) confirmed by TBB and 26 cases(100％) confirmed by TBNA.And there was statistically significant difference was observed between the diagnosis by TBB and TBNA (P =0.01) Sixteen cases belonged to the lumen external pressure type with 2 cases(12.5％) were confirmed by TBB and 16 cases(100％) confirmed by TBNA.And there was statistically significant difference was observed between the diagnosis by TBB and TBNA (P =0.02).Forty-seven cases had luminal stenosis combined with mucosal diffuse lesions with 25 cases(53.2％) confirmed by TBB and 40 cases(85.1％) confirmed by TBNA.And there was statistical difference was observed between the diagnosis of TBB and TBNA(P =0.04).Twenty-eight cases had mixed type central lung cancer with 27 cases(96.4％) confirmed by TBB and 28 cases(100％) confirmed by TBNA.No statistically significant difference was observed between the diagnosis of TBB and TBNA (P =0.87).Conclusion According to endoscopic performance of central lung cancer,accurate and reasonable choice of biopsy method can minimize inspection costs on the basis of maintaining positive rate of diagnosis in order to achieve optimal titer.

Objective To investigate the appropriate time of replacing drainage bags after trans-cervical resection of adhesion(TCRA) surgery.Methods Totally 156 patients underwent TCRA were randomly divided into three groups:Group A,the drainage bags were replaced daily;Group B,the drainage bags were replaced every 3 days;Group C,the drainage bags were never replaced.The drainage bags were removed on the 6th day after TCRA for all groups.Bacterial culture results from the balloon surface of drainage bag,outer cervical orifice,and the connector of drainage bag for 3 groups were observed.The positive rates of bacterial cultures were analyzed statistically.Results The bacterial culture results from the drainage bag connector of 3 groups indicated that the positive rate of Group C was significantly lower than that of other two groups(P＜0.05).The bacterial culture from the drainage bag balloon surface also indicated the same result,but the result did not showed statistically significant difference(P＞0.05).There was no statistically significant difference in the culture result firom outer cervical orifice and the volume of drainage fluid within three groups(P＞0.05).Conclusion It is indicated that the patient may enjoy a lower rate of bacterial infection without replacing any drainage bags connected to balloon drainage tubes placed after TCRA within 6 days.We suggest that the intrauterine drainage hags may be kept without replacement until direct removing the drainage tube on day 6 after TCRA.

Objective Mannitol-poly(D,L-lactide-co-glycolide) (M-PLGA),a star-shaped biodegradable polymer,was synthesized with the intent in this research,to provide novel nanoformulation for cervical cancer chemotherapy.Methods The novel star-shaped copolymer was prepared by ring-opening polymerization,and characterized by NMR.The docetaxel-loaded M-PLGA nanoparticles,prepared by modified nano-precipitation method,were observed to be near-spherical shape with narrow size distribution.Results The CLSM results showed the uptake level of M-PLGA NPs was higher than PLGA NPs in Hela cells.A significantly higher level of cytotoxicity was achieved by docetaxel-loaded M-PLGA NPs than that of commercial Taxotere and docetaxel-loaded PLGA NPs,indicating that the star-shaped biodegradable polymer M-PLGA could be superior to the linear polymer PLGA as a drug carrier.The study on drug loading and encapsulation efficiency also proved that star-shaped M-PLGA could carry higher level of drug than linear polymer,therefore could be more efficient for cancer treatment.Conclusions In conclusion,the star-shaped M-PLGA copolymer may be used as a potential and promising drug-loaded biomaterial applied in developing novel nanoformulations for cervical cancer therapy.