OBJECTIVE:To establish Bayesian statistics data processing method for the evaluation of bioequiavailability.METHODS:The procedures of the posterior probability calculation were simplified by means of the Excel-programmed double crossover designed Bayesian statistics analytical table for bioequiavailability.RESULTS:This method can be used as variance analysis and data processing of Bayesian statistics evalustion.CONCLUSION:The results are accurate and the application is convenient in this method.

Objective To compare the curative effects between minimally invasive and standard direct approach of pri?mary total hip arthroplasty (THA) in elderly patients. Methods One hundred and eighteen geratic patients with hip disease were divided into minimally invasive group (n=61) and standard group (n=57). All the patients were followed up from 15 months to 3 years. The length of incision, surgical duration, intraoperative blood loss, postoperative drainage, number of blood transfusion, hospitalization time and the Harris hip scores were compared between two groups. Results The length of incision, intraoperative blood loss, postoperative drainage, number of blood transfusion and hospitalization time were signifi?cantly lower in minimally invasive group than those in standard group, but the Harris hip scores were significantly higher 3 months after the operation in minimally invasive group than those in standard group (P<0.05). First intention wound healing was found in all patients in two groups. The postoperative complications were significantly lower in minimally invasive group than those in standard group (3.28%vs 15.79%, P<0.05). Conclusion Compared with standard incision of primary total hip arthroplasty , minimally invasive direct lateral approach has its advantages, including small surgical trauma, less hospitaliza?tion time, fewer complications, and which can help restore joint function quickly, making the curative effect satisfied in elderly patients.

256 mg/L) are resistant to metronidazole by two tests. The level of transcripts of rdxA genes significantly reduced the total RNA in Helicobacter pylori grown with metronidazole compared to that without metronidazole. And the expression of the galE gene used as the control was unchanged. Conclusion The low expression of the rdxA gene may play a role in the development of metronidazole high resistant Helicobacter pylori isolates.

Objective To confirm the effect of lymphangiogenesis in immunological reaction on pterygium formation and development.Methods The clinical removal of pterygium material in quiescent phase,advanced phase and recurrent phase was selected.each phase with 12 cases,while 10 cases of normal bulbar conjunctiva were taken as control.5'-nueleotide enzyme-alkallne phosphat&se-double enzyme histochemistry was performed to compare the number of lymphangiogenesis in different tissues.Results Lymphangiogenesis in normal bulbar conjunctiva and quiescent phase pterygium tissues expressed little,but gray-stained lymphangiogenesis widely distributed in pterygium tissue of advanced phase and recurrent phase,and there were statistically significant differences compare those with the normal bulbar conjunctiva and quiescent phase pterygium(P=0.03 and 0.01).Conclusion Lymphangiogenesis may be involved in immunity process of pterygium pathogenesis.

With the example analysis method, this paper discussed the feasibility of flipped class to permeate the medical ethics education into medical English teaching. Practice has proved that consciously combining lan-guage teaching with the medical ethics education on various modern networks teaching platform enabled students to foster a correct view of medical ethics and to be qualified medical workers.

Objective To investigate the effects of magnetic iron-oxide (Fe3O4) nanoparticles with different skin materials on cytotoxicity against human cervix cancer HeLa cells.Methods The Fe3O4 nanoparticles were prepared by solvent-free dissolving method and then covered with poly(lactic-co-glycolic acid) (PLGA) and cholic acid (CA)-PLGA respectively.After the characterization,the uptake of nanoparticles by HeLa cells was observed by laser scanning confocal microscopy,and thiazolyl blue tetrazolium bromide (MTF) assay was used to assess the cytotoxcity of the Fe3O4-loaded nanoparticles against HeLa cells.Results The size of single Fe3O4 nanoparticle was about 7 nm.The Fe3O4-loaded PLGA and CA-PLGA nanoparticles were spherical in shape under transmission electron microscope,around 200 nm in diameter,and the theoretical drug-loading capacity was 10％.At the same Fe3O4 nanoparticles concentration of 25 μg/ml,the Fe3O4-loaded CA-PLGA nanoparticles showed less toxicity than Fe3O4-loaded PLGA nanoparticles.Conclusions Star-shaped copolymer CA-PLGA is promising in decreasing cytotoxicity of magnetic Fe3O4 nanoparticles and expanding its application in living organisms.

8 mg/L). The rdxA gene fragment (886 bp) of H. pylori was amplified by PCR and then sequenced. Finally polymorphism analysis was performed in the rdxA gene sequence from those strains resistant or susceptible to Mtz and standard strains H.pylori 26695. Results A total of 47 H.pylori strains were isolated from 51 patients positive for rapid urease test. Mtz resistance rate was 34% by E-test method and 31.9% by breakpoint susceptibility testing. Among them 15 strains were resistant to Mtz by the two tests. The rdxA genes of both 15 strains resistant to Mtz and 8 strains susceptible to Mtz were sequenced. Compared with that of strain H.pylori 26695, the sequence of rdxA gene nucleotide in 15 H.pyloristrains resistant to Mtz showed similarity ranging from 90% to 95% and being 92.5% on average, and the sequence of rdxA gene nucleotide in susceptible strains showed similarity ranging from 94% to 95% and being 94.5% on average. In most resistant strains, the rdxA genes occurred point mutation, single nucleotide insertion or deletion, however, the insertion or deletion of large nucleotide fragment were rare, and mutational site were variable. Conclusions The rdxA gene mutation in H.pylori strains resistant to Mtz may result from point mutations in several bases or insertional mutations or deletional mutations in one base, without insertion or deletion of large neuclectide fragment, and mutational position being variable.

Objective To explore the effect of lidocaine combined with ropivacaine in penile dorsal nerve block for pediatric circumcision.Methods 130 children with pediatric circumcision surgery were divided into observation group and control group by the digital meter method,65 cases in each group.The control group was taken inhaled sevoflurane composite static note ketamine surgery,the observation group was given ropivacaine combined with intravenous infusion of ketamine for penile dorsal nerve block anesthesia.The anesthesia effects of the two groups were recorded.Results In the observation group,10 min after surgery,the rhythm of the heart was (98.47±10.88)times/min,central arterial pressure was (65.13±7.78)mmHg.In the control group,10min after surgery,the rhythm of the heart was (121.78±15.23)times/min,central arterial pressure was (85.27±12.31)mmHg,the differences between the two groups were statistically significant(t=10.040,11.150,all P＜0.05).In the observation group,the used ketamine,waking up time,pain score,incidence rate of pain and irritability were (41.38±11.28)mg,(5.54 ±1.03)min,(2.11±1.13)points,7.69％,respectively,which in the control group were (71.47±16.82)mg,(13.67±2.57)min,(3.89±1.79)points,26.15％,respectively,the differences between the two groups were statistically significant(t=11.978,23.673,6.779,χ2=7.878,all P＜0.05).Conclusion The anesthesia effect of lidocaine combined with ropivacaine in penile dorsal nerve block for pediatric circumcision is stable,safe and reliable,it is worthy of popularization and application in clinic.

Aim Toinvestigatetheinducementeffect of isatin on apoptosis of breast cancer cell line MCF-7 , andexploreitsdetailedmechanism.Methods MCF-7 cell lines were exposed to isatin at different concentra-tions(0,50,100,200 μmol·L-1 )for 48 h.Apop-totic features were demonstrated by nuclei staining with Hoechst 33258.Bcl-2,Bax and p53 mRNA were ana-lyzed by RT-PCR.Caspase-9 activation and mitochon-drial depolarization were assayed by flow cytometry. Bcl-2,Bax,p53 and cytochrome c proteins were ana-lyzedbyWesternblot.Results Isatininducesapopto-sis of MCF-7 cells.Furthermore,Bcl-2 expression was decreased and the ratio of Bcl-2 to Bax was significant-ly decreased by isatin.While,p53 expression relative-ly decreased.The mitochondrial transmembrane poten-tial was markedly reduced and the release of cyto-chrome c into the cytosol was increased after treatment with isatin.Simultaneously,caspase-9 was activated. Conclusions Isatinsignificantlyinducedtheapopto-sis of MCF-7 cells in vitro.These results strongly sug-gest that the p53 dependent mitochondrial pathway is involved in apoptosis.

OBJECTIVE:To estimate the cost-effectiveness of4therapeutic regi mens in the treatment of candidal vaginitis.METHODS:182out-patients with candidal vaginitis in the hospital where the authors worked were randomly di?vided into4groups:baofukang suppository group(A),miconazole suppository+miconazole cream group(B),povidone Iodine ointment group(C),and luohuazizhu suppository group(D),medical economy was applied to analyze the cost-effectiveness.RESULTS:The costs in4groups were56.2yuan,69.4yuan,75.3yuan,and53.9yuan respectively;The effective rates were93.3%,91.3%,91.3,and80.0%respectively;The ratios of cost to effectiveness were0.60,0.76,0.82,and0.67re?spectively;Compared with D,the added cost-effectiveness ratio of A,B,C are0.17,1.37and1.89respectively.CONCLU?SION:Baofukang suppository is a preferable drug to treat candidal vaginitis.