In recent years,gut microecology has attracted increasing attention because of its close relationship with the development and pro-gression of various liver diseases and associated complications.The impairment of liver function can disturb gut microecology,whereas the imbalance in gut microecology in turn promotes the aggravation of liver diseases.This paper reviews the relationship between gut microecolo-gy and various liver diseases,and discusses the role of modulating gut microecology in the treatment of liver diseases.A new idea is provided for the prevention and treatment of liver diseases based on the modulation of gut microecology.

Objective To investigate the contents of cytokeratin fragment 21-1 (CYFRA21-1),cancer antigen-153(CA153) and neuron-specific enolase (NSE)in patients wuth lung cancer. Methods 60 cases with lung cancer using single and combined determination serum CYFRA21-1, CA153 and NSE levels by radioimmunoassay (RIA). Results The contents of CYFRA21-1 ,CA125 and NSE in lung cancer were significantly higher than the control group(t = 2.811,2.845,2.901,2.795,2.759,2.811,2. 852,2.799,2. 816, all P ＜ 0. 01); The content of CYFRA21-1 in squamous cell carcinoma were higher than that small cell undifferentiated carcinoma(t = 2. 214, P ＜0. 05) ;The content of NSE in squamous cell carcinoma group were lower than small cell undifferentiated carcinoma (t = 2.312,P ＜ 0.05) ;The content of CA125 in adeno-carcinoma were higher than the higher squamous cell carcinoma scales cancer(t = 2. 177, P ＜ 0. 05); The positive rate of combined detection of CYFRA21-1 + CA153 + NSE (86. 4%)was significantly higher than the single test(x2 =3.786,P ＜0.05) ;The combined detection of sensitivity and negative predictive value was significantly higher than single detection (x2 = 3. 846,3. 877, all P ＜ 0. 05).Conclusion The combined determination could make up for missed diagnosis, and had some clinical value.

OBJECTIVE:To systematically review the efficacy of α-lipoic acid versus epalrestat in the treatment of diabetic pe-ripheral neuropathy(DPN),and provide evidence-based reference for clinic. METHODS:Retrieved from CJFD,CBM,VIP,Wan-fang Database and PubMed,randomized controlled trials(RCT)about α-lipoic acid versus epalrestat in the treatment of DPN were collected,and the study was screened by inclusion and exclusion criteria. Meta-analysis was performed by using Rev Man 5.3 soft-ware after data extraction and quality evaluation by Cochrane software. RESULTS:Totally 6 RCTs were enrolled,involving 408 pa-tients. Results of Meta-analysis showed,compared with epalrestat,there were no significant differences in the total effective rate [RR=0.98,95%Cl(0.84,1.15),P=0.81],motor nerve conduction velocity [median nerve:MD=1.02,95%Cl(-1.10,3.14),P=0.34;common peroneal nerve:MD=0.23,95%Cl(-1.11,1.58),P=0.73] and sensory nerve conduction velocity [median nerve:MD=1.10,95%Cl(-0.39,2.59),P=0.15;common peroneal nerve:MD=0.95,95%Cl(-1.47,3.36),P=0.44] in α-lipoic acid group. CONCLUSIONS:The efficacy of α-lipoic acid is similar to epalrestat in the treatment of DPN,as well as the motor nerve conduction velocity and sensory nerve conduction velocity of median nerve or common peroneal nerve.

SUMMARY Breastfeeding has been recognized as the most natural and nutritious way of feeding babies . Besides the nutritional , immunological and emotional benefits , breastfeeding promotes a healthy stomatog-nathic system .First of all, the nutrients and minerals in maternal milk are easy to be absorbed by the infants, which contributes to the mineralization of the teeth , and suppress the propagation of bacteria on the teeth.Though the jury is still out on whether breastfeeding can prevent Early Childhood Caries (ECC), it is definite that we should pay attention to feeding at night and the oral hygiene of the babies . Secondly , the method of feeding is closely bound up with the development of dentition and jaw .Breast-and bottle-feeding involve different orofacial muscles , which possibly have different effects on the harmo-nic growth of maxilla and dental arches .Meanwhile , breathing , swallowing and mastication should be developing in harmony , and differences exist in the learning of the coordinated movement between breast feeding and bottle feeding children .Bottle feeding had been proved to be closely related with the non-nutritive sucking habits which can cause malocclusion .At last, it should be pointed out that breast fee-ding should be the only feeding source in the first 6 months of life, then supplementary foods should be added .And prolonged bottle feeding should be avoided .We can see that breast feeding is definitely good for the infants , but the reality is not optimistic in our country .

AIM: To study the dynamic expression of protease - activated receptors - 1 (PAR- 1 ) after intracerebral hemorrhage (ICH) and the influence of Nao Xue Kang Tablet (NXKT) on it's expression. METHODS: 72 Wistar rats were divided into normal group, ICH model groups (ICH, 6 h, 24 h, 3 d, 7 d), Nao Xue Kang groups (NXKT, 6 h, 24 h, 3 d,7 d).ICH models were produced with the induction of collagenase typeⅦ - S, except normal group. Immunohistochemical method was used to detect PAR- 1 protein and RT- PCR technique was used to detect PAR- 1 mRNA in brain tissues around the haematoma at different time points of different groups. RESULTS: PAR - 1 protein and mRNA were mildly positive in normal group. In ICH model groups, intensity of PAR - 1 expression started to increase at 6 h, and further increased at 24 h. PAR - 1 expression reached the peak at 3 d and began to descend. At 7 d the decent was obvious. At 6 h, 24 h, 3 d, and 7 d time points, the PAR-1 protein positive cell number and PAR- 1 mRNA absorbance ratio in ICH model and NXKT groups were significantly higher than those in normal group ( P ＜0.05 or P ＜ 0.01). The PAR- 1 protein positive cell number and PAR- 1 mRNA absorbance ratio in NXKT group were significantly lower than in ICH model group ( P ＜ 0.05 or P ＜ 0.01 ). CONCLUSION: After ICH,PAR - 1 is continuously activated because of the stimulation of thrombin. Action of thrombin after ICH may be mediated by PAR- 1; NXKT may inhibit the activation of PAR- 1, so the praxiology is improved. This may be one of the main mechanisms that NXKT could facilitate the recovery of nervous function.

OBJECTIVE:To prepare adriamycin-loaded PLGA-PLL-PEG nanoparticles,and study its antitumor activity. METH-ODS:PLGA-PLL-PEG with the polymerization of PLGA-PLL and activated polyethylene glycol was used as carrier for adriamy-cin,and adriamycin-loaded PLGA-PLL-PEG nanoparticles were prepared. The shape size,particle size distribution,adriamycin con-tent of nanoparticles were detected,drug loading and encapsulation efficiency were calculated. Cumulative release rate (Q) of nanoparticles and adriamycin within 144 h and its proliferation inhibition rate on breast cancer HeLa cells were compared,and half inhibitory rate (IC50) was calculated. RESULTS:Prepared adriamycin-loaded PLGA-PLL-PEG nanoparticles were regular circular with good dispersion and no adhesion. The average particle size was (136.7 ± 9.3) nm (n=5),average encapsulation efficiency was(76.67±8.63)%,average drug loading was(3.86±0.55)%(n=3). Q12 h of adriamycin had reached 100%;Q12 h of adriamy-cin-loaded PLGA-PLL-PEG nanoparticles was 52.9%,Q144 h was 81.2%. The inhibitory rate of adriamycin-loaded PLGA-PLL-PEG nanoparticles on HeLa cells increased slowly than adriamycin;IC50 were 1.844,0.345 μg/mL,respectively. CONCLUSIONS:Adri-amycin-loaded PLGA-PLL-PEG nanoparticles are prepared successfully,showing good sustained-release effect and more significant inhibitory effect than adriamycin.

AIM: To study the dynamic expression of protease-activated receptors-1 (PAR-1) after intracerebral hemorrhage (ICH) and the influence of Nao Xue Kang Tablet (NXKT) on it's expression. METHODS: 72 Wistar rats were divided into normal group, ICH model groups (ICH, 6 h, 24 h, 3 d, 7 d), Nao Xue Kang groups (NXKT, 6 h, 24 h, 3 d,7 d). ICH models were produced with the induction of collagenase typeⅦ-S, except normal group. Immunohistochemical method was used to detect PAR-1 protein and RT-PCR technique was used to detect PAR-1 mRNA in brain tissues around the haematoma at different time points of different groups. RESULTS: PAR-1 protein and mRNA were mildly positive in normal group. In ICH model groups, intensity of PAR-1 expression started to increase at 6 h, and further increased at 24 h. PAR-1 expression reached the peak at 3 d and began to descend. At 7 d the decent was obvious. At 6 h, 24 h, 3 d, and 7 d time points, the PAR-1 protein positive cell number and PAR-1 mRNA absorbance ratio in ICH model and NXKT groups were significantly higher than those in normal group (P

Objective To investigate the effects of exercise on behavior and serum cortisol levels in depression caused by chronic,unpredictable,mild stress(CUMS).Methods Forty-eight male Sprague-Dawley rats were randomly divided into 3 groups:A,B and C.Each group was then divided into 3 subgroups:a blank control subgroup,a model subgroup,and an intervention subgroup.CUMS such as unpredictable forced swimming in cold water,electric stimulation of the animals'feet and shaking of the cage was used to establish a model of depression.Swimming exercise was administered as a therapeutic intervention.In group A it was administered while the animals were under stress,while in groups B and C,exercise was administered before and after the depression model had been established.Open-field test and fluid consumption data were recorded during the whole procedure.At the end of the experiment,radioimmunity assays were used to measure the levels of serum cortisol.Results Rats exposed to CUMS showed reduced water consumption;a preference for sucrose solutions;and decreased squares crossed,less rearing and increased bowel movement in open-field test.All of these changes were mitigated by the exercise performed while the animals were under stress,but not by exercise before or after the depression model had been established.Rats exposed to CUMS showed significant increases in serum cortisol,which was mitigated by exercise prior to and during stress.Conclusion Different exercise routines have different effects on behavior and serum cortisol levels in depressive rats under chronic unpredictable mild stress.Exercise has cumulative effects in decreasing the serum level of cortisol,but has only immediate effects on the behavior of depressive rats.

Objective: TO study the change of substance P(SP) level in Plasma and its relationship with ankylosing spondylitis(AS).Method: Radioimmunoassay. Results: The author observed that the level of SP in Plasma of AS is singificantly higher than that of healthy controls. SP level in AS patients during active stage is higher than in those during inactive stage. Conclusion: Increased SP may play an importantrole in the pathogenesis Of AS.

Objective To investigate the effect of Tanreqing injection on pulmonary function, blood gas index and clinical symptoms in patients with chronic obstructive pulmonary disease.Methods 90 cases of chronic obstructive pulmonary disease patients from January 2010 to December 2013 in Xinjiang Tuha Petroleum Hospital were selected as the research object.They were randomly divided into the observation group and the control group, 45 cases in each group.The control group received routine western medicine, on the basis of this, the patients in the observation group were treated with Tanreqing injection.Before treatment and 15 days after treatment, compared the two groups of patients with pulmonary function , blood gas index and the improvement of clinical symptoms.Results Before treatment, there were no significant differences between the two groups of patients with FEV1 , FVC, FEV1/FVC and other pulmonary function index, PaO2 , PaCO2 , SaO2 and other blood gas index and the scores of clinical symptoms.15 days after treatment, the two groups of patients with FEV1 , FVC, FEV1/FVC and other indicators of pulmonary function significantly increased, while its PaO2 , SaO2 levels also increased significantly, the level of PaCO2 in the two groups was decreased, and the clinical symptom scores were significantly decreased.However, the improvement rates of the observation group were significantly higher than that of the control group, the differences between the groups were statistically significant (P<0.05).Conclusion On the basis of conventional western medicine treatment, add Tanreqing injection to treat chronic obstructive pulmonary disease, can significantly improve the pulmonary function and blood gas index level, the clinical symptoms have been effectively relieved.