Objective To explore the influence of different testing methods on detecting value of to-tal cell volume. Methods 60 reusable dialyzers(low-flux dialyzer and high-flux dialyzer were 30 respec-tively) were enrolled. They were divided into the traditional testing group, the modified testing group and the automatic dialyzer reuse group with 20 dialyzers in each group according to different detecting methods, the results underwent analysis. Results The basic total cell volume of the traditional testing group, the modified testing group and the automatic dialyzer reuse group were (100.10±0.25) ml, (100.13±0.50) ml and (102.00±1.41) ml in 6LR dialyzer, while in 17R dialyzer, they were (113.67±1.30) ml, (118.67±1.30) ml and (119.93±1.77)ml respectively. The total cell volume of the dialyzer reuse in the traditional testing group, the modified testing group and the automatic dialyzer reuse group were (95.20±7.76) ml, (96.20± 7.22) ml and (102.80±4.26) ml in 6LR dialyzer, while in 17R dialyzer, they were (100.00±11.48) ml, (114.35±3.31 ) ml and (117.07±2.96) ml respectively. There was significant difference between the tradi-tional and the modified testing groups in high-flux dialyzers reuse. Conclusions The modified testing method can improve the accuracy of the testing of total cell volume in high-flux dialyzer reuse.

BACKGROUND: The investigation of culturing, passaging and establishing human limbal stem cells can strengthen the recognition of the stem cells and provide the enough cellular reserve for the basic and clinical research of limbal stem cell transplantation.OBJECTIVE: To explore a method of pessaging and establishing cell line of human limbal stem cells cultured in vitro.DESIGN: Randomized controlled observation.SETTING: Gannan Medical College.MATERIALS: The experiment was performed at Scientific Center of Gannan Medical College and the National Key Laboratory of Ophthalmology Hospital Affiliated to Sun Yat-Sen University from June 2003 to April 2004. Fresh human limbus corneae were isolated from two healthy donors. Procedures were performed according to the informed consent of the donors. Main reagents contained RPMI-1640 (Sigma R8755, containing L-glutamine) and 200 g/L fetal calf serum (FCS) (Gibco 16140-071). DMEM medium, chondroitin sulfatase and human epidermal growth factor (hEGF) were purchased from Sigma Co. USA; HEPES and DMSO were bought from Gibco, USA; 100% glycerinum was purchased from Yunjia Huangpu Pharmaceutical Product Limited Company, PRC; glutaraldehyde was bought from E.Merk, Germany; Alcohol, chlorhydric acid, acetone and methyl aldehyde were purchased from Beijing Chemical Agent Company, PRC; 0.25% parenzyme was bought from Shanghai Xinhua Pharmaceutical Factory, PRC.Above-mentioned reagents were analytical pure grade.METHODS: After digestion, human limbal tissues in limbal basilar part with an abundant pigment were cultured in the culture flask containing RPMI-1640 and 200 g/L FCS and in culture dish containing amniotic extracellular matrix (AECM) as the cultural supporter. Primary and passage cells were observed under light microscope and scanning electron microscope (SEM). The revival ratio of stem cell refrigeration of every generation was calculated by the trypanblau exclusion experiment.MAIN OUTCOME MEASURES: ① Observational results of limbal stem cells during the primary culture and serial subcultivation in vitro, and ② revival ratio of stem cell refrigeration.RESULTS: ①Findings of primary culture: Most limbal stem cells in the culture flask had the adherence and were arrayed uniformly sparsely to form monolayer and adhered to the bottom of culture flask under the inverted phase contrast microscope after 1-day culture. ② Findings of serial subcultivation: After human epidermal growth factor (hEGF) was added into the second passage, cells were scattered into the monolayer and adhered to grow quickly.Morphological variability of all the cells increased obviously when passage the 30th generation. The cellular volume was obviously increasing, and the round or irregular round cells gathered together. The 33rd generation human limbal stem cells still could vigorously differentiate, proliferate and grow in ACEM. ③ The revival ratio of stem cell refrigeration was 82.2%.CONCLUSION: The human limbal stem cell lines were preliminarily established by culturing and freezing the cells of 33 generations in vitro. The human limbal stem cell lines preferred to grow in the culture dish containing AECM as the cultural supporter.

Objective To investigate the efficacy and feasibility of preimplantation genetic diagnosis (PGD) with human leukocyte antigen (HLA) matching for beta-thalassemia. Methods A total of 43 referred beta-thalassemia couples, with at least on child in need of hematopoietic stem cell transplantation (HSCT), underwent PGD for HLA matching at the First Affiliated Hospital of Sun Yat-sen University from 2010 to 2015. PGD cycles of these couples were retrospectively analyzed, and 15 infants born from PGD-HLA were followed up. Results A total of 84 oocyte retrieval cycles were performed, providing 14±7 oocytes per cycle. Fifty nine embryos biopsied cycles were done, including 24 cleavage stage and 35 blastocyst stage biopsy cycles. In cleavage stage, 259 embryos were biopsied, 93.4% (242/259) of them with conclusive molecular diagnosis, and the percentage of unaffected embryos (normo-homozygote and heterozygote) was 71.4%(185/259). The percentage of HLA matched unaffected embryos was 9.3%(24/259). In blastocyst stage, 306 embryos were biopsied, while 93.8% (287/306) of them were conclusive, and the percentage of unaffected embryos was 70.6% (216/306). The percentage of HLA matched unaffected embryos in blastocyst stage biopsy was 14.4%(44/306), which was higher than in cleavage stage biopsy (P<0.05). Forty three female carriers underwent 48 embryo transfer cycles including 3 fresh and 45 frozen-thawed embryo transfer cycles. Three fresh embryo transfer cycles were done after cleavage stage biopsy, resulted in a birth of healthy twins born at 36 weeks′gestation. All the embryos were frozen after blastocyst biopsied. Totally, 54 frozen-thawed embryos that were transferred in 45 frozen-thawed embryo transfer cycles included 25 embryo from cleavage stage biopsy and 29 embryo from blastocyst stage biopsy, and 42 of them were HLA matched. Clinical pregnancy rate and implantation rate per cycle in frozen-thawed embryo transfer were 38%(17/45) and 37%(20/54) respectively. A total of 15 infants were born, 2 were from a fresh embryo transfer cycle, and 13 were from frozen-thawed embryo transfer cycles. Results of prenatal diagnosis from delivered cases were matched to that of PGD. Four sick children have been cured by HSCT from these HLA matched born siblings. Conclusion PGD with HLA matching is an established method for conceiving a child who may donate hematopoietic stem cells to save an ill sibling.

Parkinson's disease (PD) is a kind of common degenerative diseases of the nervous system. Levodopa replacement therapy is the most important clinical treatment method. However, long-term use can lead to the occurrence of dyskinesia and seriously affect the quality of life of patients. The current treatment methods for PD dyskinesia include drug therapy and non-drug therapy; most patients with dyskinesia can got improvement well by adjusting the dosage of drugs, changing the methods of administration, combination medication, or surgical treatments. Based on this, this article reviews the latest research on the treatment of PD dyskinesia to further help clinical colleagues effectively treat PD dyskinesia.

Objective To evaluate the effect of virtual reality(VR)on cognitive function and quality of life in patients with Parkin-son's disease. Methods A systematic search of CBM,CNKI,Wanfang data,VIP,Cochrane Library,Web of Science,Embase,and PubMed was carried out to identify randomized control trials(RCT)about the effect of VR technology on pa-tients with Parkinson's disease from inception to February 29th,2024.The control group received routine cogni-tive training,balance training or physical therapy,and the experimental group received VR technology.The quali-ty of articles was evaluated using the Cochrane Collaboration's 5.1.0 RCT risk assessment tool for bias.The meta-analysis was performed using Revman5.4.GRADE was used to evaluate the evidence quality of outcome indica-tors. Results A total of 13 literatures involving 426 patients were included.Allocation concealment and blind methods were not described in most literatures,and selective reporting of research results or other biases was unclear.VR tech-nology could improve the Motreal Cognitive Assessment(MoCA)score(MD=1.11,95%CI 0.31 to 1.90,P=0.006),Trail Making Test(TMT)-A score(MD=-6.25,95%CI-11.71 to-0.78,P=0.030)and depression scale score(SMD=-0.56,95%CI-0.95 to 0.18,P=0.004)of patients with Parkinson's disease;however,it did not improve TMT-B score(MD=-6.01,95%CI-28.16 to 16.14,P=0.590),Unified Parkinson's Disease Rat-ing Scale(UPDRS)-Part II score(MD=-2.11,95%CI-4.97 to 0.75,P=0.150)and Parkinson's Disease Ques-tionnaire(PDQ-39)score(MD=-0.92,95%CI-4.03 to 2.19,P=0.560).For quality of evidence,MoCA score,UPDRS-Part II score and PDQ-39 score were low,and depression score and TMT score were moderate. Conclusion VR technology can improve the cognitive function and depression of patients with Parkinson's disease;how-ever,no significant improvement is found in activities of daily living and quality of life.

Objective:To detect the concentration of various cytokines in aqueous humor of patients with diabetes retinopathy (DR) with Luminex liquid chip, and analyze the relationship between the cytokines and the occurrence and development of DR.Methods:A cross-sectional study was conducted.Sixty-three DR patients (97 eyes) treated with anti-vascular endothelial growth factor (VEGF) drugs in the First Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine from March 1, 2019 to December 31, 2019 were enrolled as DR group, including 38 nonproliferative DR (NPDR) eyes in NPDR group and 59 proliferative DR (PDR) eyes in PDR group, 39 eyes in photocoagulation group and 58 eyes in non-photocoagulation group.Twenty-seven patients (31 eyes) hospitalized for cataract surgery at the same time were collected as the control group.Aqueous humor was extracted during the operation, and Luminex liquid chip was used to detect the concentrations of vascular endothelial growth factor-A (VEGF-A), placental growth factor (PLGF), platelet-derived growth factor (PDGF)-AA, PDGF-BB, angiopoietin-like protein 4 (ANGPTL4), interleukin-6 (IL-6), IL-8, IL-1β, monocyte chemotactic protein-1 (MCP-1), intercellular cell adhesion molecule-1 (ICAM-1) and tumor necrosis factor-α (TNF-α) in aqueous humor.The concentrations of various cytokines of different groups were compared, and the correlation among various aqueous humor cytokines was analyzed by Spearman rank correlation analysis.This study adhered to the Declaration of Helsinki.The study protocol was approved by the Ethics Committee of First Affiliated Hospital of Guangzhou University of Chinese Medicine (No.Y[2019]230). Written informed consent was obtained from each patient.Results:The concentrations of VEGF-A, PLGF, PDGF-AA, ANGPTL4, IL-6, IL-8, MCP-1 and ICAM-1 in DR group were significantly higher and the concentration of IL-1β was significantly lower than those of control group ( Z=-4.747, -5.164, -3.373, -8.062, -4.535, -5.954, -5.098, -3.228, -5.954, all at P<0.01). The concentrations of VEGF-A, PLGF, PDGF-AA, ANGPTL4, IL-6, IL-8 and MCP-1 of the photocoagulation and non-photocoagulation groups were higher and the concentration of IL-1β was significantly lower than those in the control group (all at P<0.017). The concentration of ICAM-1 in the photocoagulation group was significantly higher than that in the control group ( P<0.017). The concentrations of PLGF, PDGF-AA and ANGPTL4 of PDR group were higher than those of NPDR group, and the differences were statistically significant ( Z=-2.291, -3.396, -2.276, all at P<0.05). VEGF-A was positively correlated with the other cytokines except ICAM-1 ( rs=0.237-0.540, all at P<0.05). ANGPTL4 was positively correlated with the other cytokines except IL-1β ( rs=0.361-0.733, all at P<0.01). Conclusions:The occurrence and development of DR are closely related to VEGF family, PDGF family, ANGPTL family and inflammatory factors.The concentrations of PLGF, PDGF-AA and ANGPTL4 are higher in PDR eyes.There are close and complex relationships among a variety of cytokines in the eyes of DR patients.

Objective:To systematically sort out the development trajectory of the research field of health industry,discuss the research hotspots and frontier topics in depth,and reveal the topics that should be focused on in the future research of the domestic health industry.Methods:Using CiteSpace Bibliometric Visualization Software,1 488 core journal articles in the field of health industry from 1994 to 2023 in CNKI Database were used as research samples to systematically analyze the development trend,research themes,hot issues and cutting-edge research of the health industry.Results:The number of papers published from 1994 to 2023 showed a steady growth trend on the whole.The author-institution partnership was relatively independent and loose,and there was no obvious network of collaboration.Meanwhile,the research hotspots in this field at the current stage mainly focused on the integrated development of"health industry+",health care industry,smart elderly care,health service industry chain,elderly care service system,and the background of healthy China.Conclusion:With the change of residents'cognition and demand for health,the focus of research has begun to transform from the traditional medical treatment model to the new business model of health industry of"managing health,preventing diseases,and focusing on residents'health".Based on the in-depth analysis of the existing research sample literature of the health industry,in order to effectively promote the high-quality and sustainable development of the health industry,the research field should focus on the business model of the health industry in the era of digital economy in the future,formulation and management of industry standards for the health industry,and empirical research at the micro level in the field of health industry research.

Objective:To systematically sort out the development trajectory of the research field of health industry,discuss the research hotspots and frontier topics in depth,and reveal the topics that should be focused on in the future research of the domestic health industry.Methods:Using CiteSpace Bibliometric Visualization Software,1 488 core journal articles in the field of health industry from 1994 to 2023 in CNKI Database were used as research samples to systematically analyze the development trend,research themes,hot issues and cutting-edge research of the health industry.Results:The number of papers published from 1994 to 2023 showed a steady growth trend on the whole.The author-institution partnership was relatively independent and loose,and there was no obvious network of collaboration.Meanwhile,the research hotspots in this field at the current stage mainly focused on the integrated development of"health industry+",health care industry,smart elderly care,health service industry chain,elderly care service system,and the background of healthy China.Conclusion:With the change of residents'cognition and demand for health,the focus of research has begun to transform from the traditional medical treatment model to the new business model of health industry of"managing health,preventing diseases,and focusing on residents'health".Based on the in-depth analysis of the existing research sample literature of the health industry,in order to effectively promote the high-quality and sustainable development of the health industry,the research field should focus on the business model of the health industry in the era of digital economy in the future,formulation and management of industry standards for the health industry,and empirical research at the micro level in the field of health industry research.

Objective:To systematically sort out the development trajectory of the research field of health industry,discuss the research hotspots and frontier topics in depth,and reveal the topics that should be focused on in the future research of the domestic health industry.Methods:Using CiteSpace Bibliometric Visualization Software,1 488 core journal articles in the field of health industry from 1994 to 2023 in CNKI Database were used as research samples to systematically analyze the development trend,research themes,hot issues and cutting-edge research of the health industry.Results:The number of papers published from 1994 to 2023 showed a steady growth trend on the whole.The author-institution partnership was relatively independent and loose,and there was no obvious network of collaboration.Meanwhile,the research hotspots in this field at the current stage mainly focused on the integrated development of"health industry+",health care industry,smart elderly care,health service industry chain,elderly care service system,and the background of healthy China.Conclusion:With the change of residents'cognition and demand for health,the focus of research has begun to transform from the traditional medical treatment model to the new business model of health industry of"managing health,preventing diseases,and focusing on residents'health".Based on the in-depth analysis of the existing research sample literature of the health industry,in order to effectively promote the high-quality and sustainable development of the health industry,the research field should focus on the business model of the health industry in the era of digital economy in the future,formulation and management of industry standards for the health industry,and empirical research at the micro level in the field of health industry research.

Objective:To systematically sort out the development trajectory of the research field of health industry,discuss the research hotspots and frontier topics in depth,and reveal the topics that should be focused on in the future research of the domestic health industry.Methods:Using CiteSpace Bibliometric Visualization Software,1 488 core journal articles in the field of health industry from 1994 to 2023 in CNKI Database were used as research samples to systematically analyze the development trend,research themes,hot issues and cutting-edge research of the health industry.Results:The number of papers published from 1994 to 2023 showed a steady growth trend on the whole.The author-institution partnership was relatively independent and loose,and there was no obvious network of collaboration.Meanwhile,the research hotspots in this field at the current stage mainly focused on the integrated development of"health industry+",health care industry,smart elderly care,health service industry chain,elderly care service system,and the background of healthy China.Conclusion:With the change of residents'cognition and demand for health,the focus of research has begun to transform from the traditional medical treatment model to the new business model of health industry of"managing health,preventing diseases,and focusing on residents'health".Based on the in-depth analysis of the existing research sample literature of the health industry,in order to effectively promote the high-quality and sustainable development of the health industry,the research field should focus on the business model of the health industry in the era of digital economy in the future,formulation and management of industry standards for the health industry,and empirical research at the micro level in the field of health industry research.