Sub-health status in the occupational population showed high incidence. Thus, it is necessary to improve the occupational groups of sub-health status, improve the health level of occupational groups. Shanghai university of traditional Chinese medicine Shuguang hospital treatment center (hereinafter referred to as disease prevention center), based on traditional Chinese medicine disease theory, did a lot of practices on occupational sub-health groups with Chinese medicine prevention and health care. Since 2014, three-year commitment to the Shanghai action plan project, the hospital has constructed a sub-health platform. This article discussed the experience of construction of sub-health platform for the construction of sub-health management system with Chinese characteristics and provided reference for the construction of sub-health management model.

Objective To explore the environmental risk factors,periconceptional folate intake and methylenetetrahydrofolate reduetase(MTHFR) gene C667T polymorphism of pregnant women on congenital heart diseases(CHD) in offspring.Methods Retrospective case-control study was carried out to investigate periconceptional folate supplementation and environmental factors in 98 parents with CHD offsprings and 101 parents with normal offsprings.The mothers'MTHFR gene C667T mutation was also identified.The possible risk factors were analyzed by simple and multiple factors Logistic regression methods.Results Six factors were related to the occurrence of CHD in the offspring:education of gestation mother,no prenatal examination,under depressed or nervous condition during pregnancy and maternal exposures to harmful substance;periconceptional folate and compound vitamin supplementation were protection factors.There were significant difference between case and control group in folate supplement(P

Objective To observe two main subtypes of MAPK family-ERK and JNK activation in HSCs and investigate the effects of the serum of Radix Salviae Militiorrhizae treated animals on the activation of ERK and JNK in HSCs. Methods HSCs were cultured in vitro. After the model of hepatic fibrosis was replicated in SD rats, Radix Salviae Militiorrhizae decoction was given via gastrogavage to the rats of treatment groups to obtain serum containing the drug. The dose was 10 times of dose per kg per day for adults in 2 divided doses for 6 consecutive days, while the same volume of 0.9% NaCl was given to the rats of non-treatment groups. on the 7th day, the routine dose was orally given again; the blood sample was drawn from the vena cava after 2 hour; the serum was isolated and inactivated with water bath at 56℃; finally, the serum was filtered to eliminate bacteria. Just before using the serum, RPMI-1640 culture medium was added to prepare culture media of 10% drug-containing serum, which was incubated with the subcultured HSCs. The experiment was divided into the following groups: A: serum of extracted from normal rats; B: medicated serum of RSM extracted from normal rats; C: serum from CCl_4-induced liver fibrosis rats; D: medicated serum of RSM extracted from CCl_4-induced liver fibrosis rats.After 24h incubation with above every group serum which were added blindly to HSCs, P-ERK and P-JNK were detected by Western blot respectively. Results Both RSM Pharmacological serums decreased P-ERK and P-JNK in HSCs significantly compared with controls (P

With the development and clinical use of the molecular targeted drugs and individualized treatment,cancer research has been focused on gene targeting diagnosis and treatment.Especially for the epidermal growth factor receptor (EGFR) signaling pathway-related genes,DNA replication-related genes,spindle apparatus format-related genes,cell metabolism-related genes and other molecular targeted detection and treatment,the polymorphism of targeting genes/molecules determines the clinical efficiency of the therapies.

microRNAs ( miRNAs) are a family of short non-cod-ing RNAs that regulate the expression of target genes by binding to complementary regions. The miRNAs expression is readily al-tered by drugs, carcinogens, hormones, stress or diseases, and that might lead to changes in the drug metabolism, pharmacoki-netics or potency. Moreover, the evaluation of drug metabolic enzyme-related miRNAs would provide useful information for per-sonalized medicine. This review describes the current knowledge on the post-transcription regulation of drug metabolic enzymes by miRNAs.

Aim To establish an analytical method for determination of guanfu base I (GFI) concentration in plasma and investigate its pharmacokinetics in rats. Methods Rats were given a 20 mg?kg~-1 dose intravenously. Blood samples were collected at various times after iv administration. The plasma concentration of GFI was determined by LC-MS. Pharmacokinetic parameters were calculated by 3p97 program.Results The method was linear in the 0.05～20 mg?L~-1 concentration range (r=~0.999 4 ). The recovery of guanfu base I was more than 80%.The intraday and interday precision, expressed as the relative standard deviation (RSD), was generally good (

Drug-induced liver injury ( DILI) is a significant rea-son of acute liver failure and is the main cause of therapeutic drugs withdrawal from the market .Multiple mechanisms can cul-minate in DILI , but metabolism and genetics play distinct roles in this process .This review will cover papers we consider have addressed these mechanisms of DILI in commonly used medica-tions for adults , and discuss the hot issues .The aim is to gener-ate discussion about the potential clinical significance among these researchs and point out the key areas for further study of DILI.

ObjectiveTo summarize the clinical features and risk factors of neonatal clavicle fracture so as to decrease the incidence of this disease.MethodsA total of 85 cases of neonatal clavicle fracture admitted to the Gynecology and Obstetrics Hospital of Fudan University between January 1, 2013 and December 31, 2015 were enrolled in this study. The full-term neonates with natural delivery by the same midwives or doctors on the same day were selected as control group with 1∶1 matched. Clinical features and risk factors of neonatal clavicle fracture based on three factors: mother factors, labor factors and neonatal factors, were analyzed retrospectively. Two independent samplest-test, rank sum test andChi-square test were used for statistical analysis.ResultsThe incidence rate of neonatal clavicle fractures was 0.14% (85/59 991). There were 45 (53%) males and 40 (47%) females; 80 (94%) cases of vaginal delivery, and 5 (6%) cases of cesarean section. According to the relationship between the location of the fracture and the position of the fetus, the fracture occurred in the anterior shoulder in 65 cases (76%) and in the posterior shoulder in 20 cases (24%);and 18 cases (21%) were in the left and 67 cases (79%) in the right. Fifty-five cases (65%) were diagnosed by physical examination, and 30 cases (35%) by X-ray. Two patients (2%) were complicated with brachial plexus injury. Twenty cases (24%) were diagnosed on the day of birth, 32 cases (38%) on the second day after birth, 16 (19%) on the third day, and four (5%) on the 4-6 days. Thirteen (15%) cases were diagnosed in the outpatient department 42 days after birth. All the fractures were cured without special treatment. Compared with the control group, the birth weight of fracture group was significantly higher [(3 646±361) vs (3 447±409) g,t=2.339], and the ratio of the cases complicated with shoulder dystocia was also higher [15% (13/85) vs 2% (2/85),χ2=9.524] (bothP<0.05). The differences in the factors of maternal age, parity, times of pregnancy, gestational age, forceps delivery, time of the second stage of labor and fetal distress between the two groups were not statistically significant.ConclusionsNeonatal clavicle fracture is related to birth weight and shoulder dystocia. Not all fractures can be diagnosed through physical examination on the day of birth, therefore, daily routine physical examinations after birth are necessary. The prognosis of neonatal clavicle fracture is good.

Objective To review the complications after endovascular aneurysm repair (EVAR) of high-risk abdominal aortic aneurysms in our medical center.Methods Fifty eight elective high-risk EVAR cases from January 2008 to December 2013 were analyzed retrospectively.Results The technical success rate was 96.6％ (56 of 58 patients).The overall complication rate in perioperative period was 32.8％ (19/58).Eleven systemic complications (19.0％),16 primary endoleaks (27.6％),7 access of site complications(12.1％) were observed.The follow-up rate was 86.2％ (51/58).During the follow up period,the mortality was 7.8％ (4/51),the re-intervention rate was 7.8％ (4/51).The overall complication rate was 29.4％ (15/51),including systemic complications (2/51),secondary endoleak (9.8％) and graft-related complications (11.8％,6/51).Conclusions Endoleak,graft-related complications continue to be the main causes of re-intervention in high-risk aneurysm patients after EVAR.

Objective To analyze the natural changes of maternal thyroid function among women with subclinical thyroid dysfunction and euthyroid women during pregnancy.Methods A total of 4 042 singleton pregnant women received routine antenatal care in the Obstetrics and Gynecology Hospital of Fudan University between April and November 2012 were enrolled.Thyroid-stimulatinghormones (TSH),freetriiodothyronine (FT3) and free thyroxine (FT4) of 7 136 samples from 4 042 singleton pregnant women were tested at 8-12+6,13-19+6,20-27+6,and 28-40 weeks of gestation and were used to establish the normal gestationalspecific reference values of thyroid function.Among 3 895 women having thyroid function tested at 8-19+6 weeks of gestation with negative thyroid antibodies,there were 93 cases of subclinical hyperthyroidism,91 cases of subclinical hypothyroidism (SCH),three cases of hyperthyroidism and 3 708 cases euthyroid.There were 1 118 women [1 607 euthyroid cases,17 cases of subclinical hypothyroidism (SCH) and 34 cases of subclinical hyperthyroidism] had thyroid function retested at 20-27+6 and 28-40 weeks of gestation,and without medicinal intervention.Analysis of variance and LSD test were used to analyze the changes of maternal thyroid function.Results (1) The reference ranges of TSH at 8-12+6,13-19+6,20 27+6 and 28-40 weeks of gestation [median (Pz5-P97.5)] were 1.32 (0.03-4.17),1.83 (0.19-4.94),2.27 (0.70-5.42) and 2.34 (0.63-5.52) mU/L respectively.(2) Without medicinal intervention,thyroid function became normal in 80％ (45/56) SCH women at 20-27+6 weeks,but 20％ (9/45) of them developed SCH again at 28-40 weeks.The thyroid function became normal in 75％ (70/93) women with subclinical hyperthyroidism at 20-27+6 weeks,but in 15％ (14/93) of them,thyroid function remained abnormal at 28-40 weeks.9.40％ (30/319) and 6.25％ (21/336) euthyroid women with TSH ≥ 3 mU/L at 8-19+6 weeks of gestation developed SCH at 20-27+6 weeks and 28-40 weeks,while 0.42％ (5/1 202) and 0.86％ (10/1 163) euthyroid women with TSH ＜3 mU/L had SCH.1.66％ (20/1 202) and 1.98％ (23/1 163) euthyroid women with TSH＜3 mU/L at 8-19+6 weeks of gestation developed subclinical hyperthyroidism at 20-27+6 weeks and 28-40 weeks of gestation.(3) In comparison between 8-19+6 weeks and 20-27+6 weeks of gestation,TSH levels increased by (0.47±0.03) mU/L in euthyroid women,and more significantly in subclinical hyperthyroidism women [(0.82±0.06) mU/L],but decreased by (1.67±0.25) mU/L in SCH women (LSD test,all P＜0.05).The FT3 levels decreased by (0.47±0.02) pmol/L in euthyroid women,and more significantly in subclinical hyperthyroidism and SCH groups [(1.02± 0.18) and (0.72±0.08) pmol/L,LSD test,all P＜0.05].FT4 decreased by (2.31 ±0.04) pmol/L in euthyroid women,and more significanly in subclinical hyperthyroidism women [(4.63± 0.62) pmol/L] (LSD test,P＜0.05),but the decrement in SCH group [(1.78±0.28) pmol/L] was similar to euthyroid women (LSD test,P＞0.05).There were no significant differences in changes of TSH,FT3 and FT4 at 20-27+6 weeks and 28-40 weeks among euthyroid women,SCH and subclinical hyperthyroidism groups (F=1.01,1.14 and 2.04,all P＞0.05).Conclusions Women with subclinical thyroid dysfunction with negative thyroid antibodies experience significantly different natural changes when compared with euthyroid women,especially before 28 weeks of gestation.