Objective To study the genetic toxicity of surfactants Tween-20?Tween-80?Span-20?BE-2. Methods To take root tip cells of Vicia faba as the subjects, treated with various concentrations of surfactants to study the effect of them on the micronucleus and chromosome aberration in root tip cells of Vicia faba. Results The frequencies of micronucleus cell induced by surfactants Tween-20?Tween-80?Span-20?BE-2 were (21.34?1.31)‰, (13.44?2.66)‰, (15.29?3.16)‰ and (7.53?2.34)‰ respectively, the frequencies of chromosome aberration induced by surfactants Tween-20?Tween-80?Span-20?BE-2 were (18.16?0.50)%, (11.05?1.18)%, (23.79?1.62)% and (22.58?0.61)%, significant differences were seen compared with the control. Conclusion The results of the present paper demonstrates that surfactants Tween-20?Tween-80?Span-20?BE-2 have the effect of mutagenic agent.

Objective To analyze and summarize the characteristics of Dyskeratosis congenita(DC)in Chinese children,so as to provide reference for early diagnosis and reasonable prevention and treatment of DC.Methods The data collected from 43 cases with DC in the domestic literature in recent 10 years,as well as the clinical data of 2 cases with DC treated at Tongji Hospital,Medical School,Tongji University,and a total of 45 cases with DC were analyzed and compared with those reported in the literatures of foreign countries.Results Based on the detailed data of 2 typical cases at Tongji Hospital,Medical School,Tongji University,and the data of 45 cases in China were summarized,so the major differences in the main characteristics of DC between Chinese Children and the foreigners were as follows:(1)Onset were earlier(mean age 4.5 years,median age of 3 years),but the diagnosis was delayed(mean age of 17.9 years,median age of 18 years).(2)The presence of skin pigmentation,nail lesions and mucosal leukoplakia,such as the proportion of the complete DC triad was higher(42/45 cases,93.3％).(3)There was an earlier onset of hematopoietic suppression(mean age 5.6 years).(4)Telomerase related gene mutation types were relatively minor,DKC1(7 cases)and TINF2(6 cases)were reported in recent years,and no other type of mutation was found.(5)Effective therapy of hematopoietic reconstitution was administered in 2 cases after allogeneic hematopoietic stem cell transplantation(allo-HSCT).The effective rates were about 70％(7/10 cases)in treating bone marrow failure with low dose androgen and low dose glucocorticoid.Conclusions DC is very common in infants in China,the clinical manifestations of triad are more typical,but the age of diagnosis was significantly delayed.Improving the understanding of DC and combination with the detection of related gene mutation may improve the early diagnosis rate and clinical efficacy with allo-HSCT or effective drug maintenance therapy,and also provide reference for propitious familial eugenics and prenatal examination.

Objective To investigate the risk factors of postpartum haemorrhage(PPH) in placenta previa. Methods A retrospective analysis of 136 cases with placenta previa from Jun.1996 to Dec. 2000 was performed. Results The incidence of placenta previa was 1.24%(136/10967) and the rate of postpartum haemorrhage was 22.06%(30/136). The associated factors of postpartum haemorrhage in placenta previa were history of multiple abortions (more than twice) , previous cesa rean section, elder age, type of placenta previa, no prenatal check, prenatal bleeding and other complications. PPH might happen when the high risk factor score was more than 4. Conclusions The relative factors of postpartum haemorrhage in placenta previa are abortions more than twice, previous cesarean section, older age, types of placenta previa, absence of prenatal check, antepartum hemorrhage and other complications. We can predict PPH by high risk factor score.

Objective To explore the environmental risk factors,periconceptional folate intake and methylenetetrahydrofolate reduetase(MTHFR) gene C667T polymorphism of pregnant women on congenital heart diseases(CHD) in offspring.Methods Retrospective case-control study was carried out to investigate periconceptional folate supplementation and environmental factors in 98 parents with CHD offsprings and 101 parents with normal offsprings.The mothers'MTHFR gene C667T mutation was also identified.The possible risk factors were analyzed by simple and multiple factors Logistic regression methods.Results Six factors were related to the occurrence of CHD in the offspring:education of gestation mother,no prenatal examination,under depressed or nervous condition during pregnancy and maternal exposures to harmful substance;periconceptional folate and compound vitamin supplementation were protection factors.There were significant difference between case and control group in folate supplement(P

Objective To investigate the role of human leukocyte antigen-G ( HLA-G ) on the invasion and the molecular mechanism involved in this cellular progress in HTR-8/SVneo cell line. Methods There were three groups: groups of transfection, negative control and blank control, which corresponding to treatment by HLA-G specific siRNA, negative siRNA and only lipofectamine 2000 using lipofection technology in HTR-8/SVneo cell line. The efficiency of down-regulated of HLA-G was detected by reverse transcription-polymerase chain reaction and western blot analysis in mRNA and protein level,respectively. Changes of p38 mitogen-activated protein kinases (p-p38MAPK)/p38MAPK protein levels and the cell invasion were respectively detected by western blot analysis and transwell test. Results ( 1 ) The mRNA levels of HLA-G transfection group, negative control group and blank control group were 0. 26 ±0. 08, 0. 71 ±0. 11, 0. 79 ±0. 07, respectively. There was significant difference between transfection group and negative control group ( P ＜ 0. 01 ), while there was no significant difference between negative control group and blank control group ( P ＞ 0. 05 ). The efficiencies of down-regulated of HLA-G were ( 69. 8 ±6. 3)%, ( 14. 9 ± 2. 2 )%, 0 in transfection group, negative control group and blank control group respectively in mRNA level. (2)In protein levels, HLA-G were 0. 20 ±0. 15, 0. 75 ±0. 12, 0. 76 ±0. 21 in transfection group, negative control group and blank control group, respectively. There was significant difference between transfection group and negative control group ( P ＜ 0. 01 ), whereas there was no significant difference between negative control group and blank control group ( P ＞ 0. 05 ). The efficiencies of down-regulated of HLA-G were (81. 1 ± 14.4)%, ( 18.0 ± 7.7)%, 0 in transfection group, negative control group and blank control group respectively. ( 3 ) The invasive number of transfection group, negative control group and blank control group were 57 ± 38,364 ± 79 and 260 ± 84, respectively, with a significant difference between transfection group and negative control group (P ＜ 0. 01 ). There was no significant difference between negative control group and blank control group ( P ＞ 0. 05 ). ( 4 ) The p-p38MAPK/p38MAPK values of the HLA-G transfection group, negative control group and blank control group were 0. 74 ±0.04, 0. 47 ± 0. 09 and 0. 36 ± 0. 21, respectively. HLA-G transfection group was significantly different compared with the other two groups( P ＜0. 01 ). (5)Without or with SB203580, the p-p38MAPK/ p38MAPK values of the HLA-G transfection group were 0. 89 ± 0. 09 and 0. 16 ± 0. 04, the values of negative control group and blank control group were 0.76 ±0.08, 0. 14 ±0.03 and 0.51 ±0.05, 0.03 ±0.01, respectively. There was significant difference between without SB203580 and with SB203580 ( P ＜ 0. 01 ). (6)Without or with SB203580, the invasive number of transfection group were 51 ± 13 and 90 ± 21 ,respectively,which was significantly different ( P ＜ 0. 01 ). The invasive number of negative control group and blank control group were 290 ± 52, 298 ± 33 and 290 ± 73, 264 ± 64, respeczively, which was no significant difference between without SB203580 and with SB203580 (P ＞ 0. 05 ). Conclusions HLA-G gene may regulate invasion of trophoblast-derived cell line HTR-8/SVneo via p38MAPK signaling pathway. The lower expression of HLA-G in trophoblast cells may lead to the occurrence of pathologic pregnancy.

OBJECTIVE: To study the effect of microRNA-205 (miRNA-205) on proliferation of laryngeal carcinoma cell line Hep-2. METHOD: The expressions of miRNA-205 in 27 cases laryngeal carcinoma tissues and adjacent normal tissues were detected by Real-time quantitative PCR, the expression of PTEN protein was detected by Western blot. The expressions of PTEN were detected by Western blot after miRNA-205 inhibitor or miRNA-205 mimics was transfected into Hep-2 cells and Hep-2 cells proliferation was measured by CCK-8 kit. RESULT: The expression level of miRNA-205 was significantly higher in laryngeal carcinoma tissues than in adjacent normal tissues (P < 0.01), and the expression of PTEN protein was lower in laryngeal carcinoma tissues than in adjacent normal tissues (P < 0.01). The proliferation rate of Hep-2 cells was decreased significantly and the expression of PTEN protein in Hep-2 cells was increased significantly after miRNA-205 inhibitor was transfected into (P < 0.01), and the proliferation rate of Hep-2 cells was increased significantly and the expression of PTEN protein in Hep-2 cells was decreased significantly after miRNA-205 mimics was transfected into (P < 0.01). CONCLUSION miRNA-205 might promote the proliferation of Hep-2 cells by regulating the expression of PTEN.
Cell Line, Tumor ; Cell Proliferation ; Humans ; Laryngeal Neoplasms ; metabolism ; pathology ; MicroRNAs ; antagonists & inhibitors ; metabolism ; PTEN Phosphohydrolase ; metabolism ; Transfection

Objective To observe two main subtypes of MAPK family-ERK and JNK activation in HSCs and investigate the effects of the serum of Radix Salviae Militiorrhizae treated animals on the activation of ERK and JNK in HSCs. Methods HSCs were cultured in vitro. After the model of hepatic fibrosis was replicated in SD rats, Radix Salviae Militiorrhizae decoction was given via gastrogavage to the rats of treatment groups to obtain serum containing the drug. The dose was 10 times of dose per kg per day for adults in 2 divided doses for 6 consecutive days, while the same volume of 0.9% NaCl was given to the rats of non-treatment groups. on the 7th day, the routine dose was orally given again; the blood sample was drawn from the vena cava after 2 hour; the serum was isolated and inactivated with water bath at 56℃; finally, the serum was filtered to eliminate bacteria. Just before using the serum, RPMI-1640 culture medium was added to prepare culture media of 10% drug-containing serum, which was incubated with the subcultured HSCs. The experiment was divided into the following groups: A: serum of extracted from normal rats; B: medicated serum of RSM extracted from normal rats; C: serum from CCl_4-induced liver fibrosis rats; D: medicated serum of RSM extracted from CCl_4-induced liver fibrosis rats.After 24h incubation with above every group serum which were added blindly to HSCs, P-ERK and P-JNK were detected by Western blot respectively. Results Both RSM Pharmacological serums decreased P-ERK and P-JNK in HSCs significantly compared with controls (P

Objective To know the drinking water quality in the rural areas in Shanxi province in order to enhance the management of drinking water and provide the scientific basis for water improvement in the rural areas. Methods 11 counties were selected based on the geographical condition in Shanxi province and 10 investigation sites were selected in each county based on the population proportion of different drinking-water types. 14 indicators were determined for every water sample. Results In the rural areas, most drinking water sources were ground water and the type of water supply was mainly non-central water supply. The indexes that exceeded the related standard limit were fluoride,arsenic and bacteria indicators. The hygienic state of central water supply was much better(?2=4.13, P

Objective To analyze the differences of time-effect and pathologic feature in the course of development of liver fibrosis between the two kinds of hepatic fibrotic animal models, which were made by CCl 4 and pig serum (PS) methods.Methods Rat models of liver fibrosis were made by using CCl 4 and PS ten week methods.At 6,10,14 and 20 weeks of model making respectively,pathological features of liver fibrosis were observed and analyzed by Masson staining and computerized image analyzing system.Results In CCl 4-induced model,fibrotic pseudolobuli developed at 6 weeks during model making, became typical at 10 weeks, and got stable thereafter.Ballooning degeneration of liver cells was outstanding,and paralleled with model making course.In PS-induced model, fibrosis occurred at 10 weeks during model making, and still developed though the model making was stopped,but ballooning degeneration of liver cells was not presented.Conclusions There was some different in time-effect relation and pathologic feature of liver fibrosis between the two methods of model making. This indicated that different mechanisms were involved in the pathogenesis of liver fibrosis. The two methods of model making should be combined to study of liver fibrosis.The prophylactic and treatment by anti-fibrotic drugs should last no less than 14 weeks.

Objective To investigate the timing for delivery in twin pregnancies to improve the perinatal outcome. Methods A multicentered and retrospective study was conducted on 655 women with twin pregnancies in 6 hospitals of Shanghai from Jan 1993 to Oct 2003. The average birthweight(BW) for both twins in different gestation, the incidence of small for gestational age (SGA) and severe neonatal asphyxia were analyzed. Results For the smaller babies in all twins, the 50 percentile of the birth weight was far behind the singletons throughout the pregnancy. But for the larger babies, it was similar to the singletons if delivered before 33 weeks, lighter than the singletons if after 33 weeks and remarkable difference was shown beyond 39 weeks. The largest BW of the larger babies was 3073 g presented at 39 weeks, while 2670 g for the smaller babies at 40 weeks after which the average BW was decreased. It reached 2555 g and 2303 g at 41 weeks for the larger and smaller babies, respectively (P