Objective To investigate the effect of transforming growth factor ?1 on secretion function of rat islet allograft in vivo and in vitro.Methods Expressing vector of rat TGF-?1 was constructed and transfected to purify Wistar rat islets. Expression of TGF-?1 and rat insulin1 gene (Ins1) in experiment and control group was detected by RT-PCR; Streptozotocin 60mg/kg intra-abdominal injection prepared the SD rat diabetic model. Insulin of cultured islets at basal and stimulated phase in vitro was detected by RIA; Islet allotransplantation was performed under recipient SD rats' renal subcapsule. The level of blood glucose was recorded before and after transplantation. The glucose tolerance was also tested.Results Expression of both TGF-?1 and Ins1 were detected in experimental group, but only Ins1 was detected in control one; Experiment and control islets secreted 3 86?1 24ng/mL and 4 13?1 45ng/mL at basal phase, 8 43?1 59ng/mL and 8 95?1 74ng/mL at stimulating phase, respectively.No significant difference was observed between two groups, but it was in groups. Experimental group' mean normal glycemia value (26 8?2 9) days was significantly longer that control group (13 2?4 5 days). Control group's glucose tolerance curve was significantly above experiment one's.Conclusion TGF-?1 can prolong the survival of islet allograft while it does not impair graft's endocrine function.

0.05) while those between GCT Ⅰ and the collection of GCT Ⅱ and GCT Ⅲ were significant ( P 0.05).Conclusion:The results indicated that neither the p16 positive rate nor the p27 positive rate could be used independently for defining grade of malignancy and estimating prognosis of malignant bone tumors though p16 and p27 might play important roles in the genesis of bone tumors.

[Objective]To study the mechanism of posterior longitudinal ligament in cervical spine through measuring the collagen and Proteoglycan and Calcium changes.[Method]Fifteen specimens of cervical longitudinal ligament from cervical spondylotic myelopathy(CSM)and ten control specimens from corpses without cervical spondylosis were obtained.The content of the collagen was measured by Weossner method.Collagen type Ⅰ and Ⅱ were measured by Enzyme-Linked Immunosorbentassay(ELISA)method.Phloroglucinol spectrophotometer to determine the change of amount of Proteoglycan.The Calcium by Methyl-Thymes-Blue(MTB)Colorimetric Method.The specimens were treated hy Hematoxylin and Eosin(HE)stain method and by Masson stain method,the pathological changes of two groups were observed through microscopy.[Result]In CSM,as compared to the control groups,there showed a decrease in the contents of the total Collagen,Collagen type Ⅰ and Proteoglycan,and increase in the content of collagen type Ⅱ.The rate of type Ⅰ/Ⅱ in CSM was lower than that in control groups.An increase in the content of the Calcium.All of which have statistic significance(P

Objective To evaluate the clinical significance of detection of serum procalcitonin(PCT),high sensitivity-C-reactive protein(hs-CRP)and leukocyte in acute exacerbation of chronic obstructive pulmonary disease(AECOPD)patients.Methods 45 patients with AECOPD and 50 patients(AECOPD group)with chronic obstructive pulmonary disease(COPD)in slationary phase (COPD group)were selected,and their serum levels of PCT,hs-CRP and leukocyte were tested.Results Before treatment,the con-centrations of PCT and hs-CRP were (3.32±1.81)ng/mL and (22.36±5.03)mg/L in AECOPD group respectively.They were significantly higher than those in COPD group with statistical differences(P<0.01).The positive predictive value of PCT and hs-CRP was 68.9% and 60.0% in AECOPD group respectively,which were significantly higher than those in COPD group(P<0.01).After antibiotic treatment,the leukocyte,PCT,and hs-CRP levels in AECOPD group were significantly lower than those be-fore treatment(P< 0.01).Conclusion In AECOPD patients,PCT and hs-CRP are good diagnostic indexes.

Objective To investigate the regulatory effects of PTEN-PI3K/AKT pathway on the apoptosis of gastric cancer MKN28 cells and the possible mechanisms.Methods The specific sequence of PTEN was transfected to MKN28 cells by eukaryotic expression vector (transfection group),and then vacant vector (negative control group) and PBS (blank control group) were transfected to the MKN28 cells,respectively.The effects of PTEN-PI3K/AKT pathway on the apoptosis of MKN28 cells and the expressions of PI3K,AKT,Caspase-3 and Caspase-9 were investigated.The growth curve and apoptosis of the MKN28 cells were detected by MTT assay and TUNEL staining,respectively,and the protein expression was detected by the Western blot.MKN28 cells which did not transfected by the PTEN were processed by inhibitor of PI3K (LY294002) (treated group),and MKN28 cells in the control group were processed by PBS.The expressions of apoptosis protein and apoptosis-related protein were detected after inhibition of PI3 K.All data were analyzed using the t test.Results The model of over-expression of PTEN was obtained and transfected into MKN28 cells.The survival of MKN28 cells in the transfection group was significantly inhibited in a time-dependant manner ( r =0.938,P ＜ 0.05 ).The mean apoptotic rate of the transfection rate was 27.86％ ± 4.78％,which was significantly higher than 0.01％ ± 0.01％ of the negative control group ( t =9.527,P ＜ 0.05 ).The protein expression of PI3K in the transfection group was 0.25 ± 0.03,which was significantly lower than 0.93 ± 0.16 of the blank control group and 0.96 ± 0.15 of the negative control group (t =7.235,8.883,P ＜ 0.05 ).The protein expression of P-AKT in the transfection group was 0.21 ± 0.03,which was significantly lower than 0.93 ± 0.13 of the blank control group and 0.91 ± 0.12 of the negative control group (t =9.347,9.802,P ＜ 0.05 ).The expressions of Caspase-3 and Caspase-9 of the transfection group were 0.86 ± 0.11 and 0.87 ± 0.12,which were significantly higher than 0.16 ± 0.03 and 0.18 ± 0.04 of the negative control group,and 0.15 ± 0.02 and 0.16 ± 0.03 of the blank control group ( t =10.634,10.999,9.448,9.942,P ＜0.05).The apoptotic rate of the MKN28 cells of the treated group was 28.60％ ± 4.50％,which was significantly higher than 0.12％ ± 0.06％ of the control group (t =10.961,P ＜ 0.05 ).The protein expression of PI3K and P-AKT of the treated group were 0.18 ± 0.02 and 0.11 ± 0.01,which were significantly lower than 0.93 ± 0.14 and 0.90 ± 0.12 of the control group (t =9.186,11.363,P ＜ 0.05 ).The protein expression of PTEN of the treated group was 1.15 ± 0.15,which was significantly higher than 0.21 ± 0.08 of the control group (t =9.577,P ＜ 0.05 ).The relative expressions of Caspase-3 and Caspase-9 of the treated group were 0.86 ± 0.12 and 0.88 ± 0.11,which were significantly higher than 0.25 ± 0.02 and 0.21 ± 0.03 of the control group (t =8.685,10.178,P ＜ 0.05).Conclusions Over-expression of PTEN may enhance the apoptosis of gastric cancer cells through inhibition of PI3K/AKT pathway.Inhibition of PI3K can enhance the expression of PTEN.PI3K and PTEN regulate the apoptosis of gastric cancer cells.

Objective To observe therapeutic effects of early scab-cutting combined with axis-like abdo-men skin flap implanting in patients with wrist deep electrical burns.Methods Retrospective study was employed to analyze 70 patients received axis -like abdomen skin flap implanting for wrist deep electrical burns.All the cases were separated with early scab-cutting group(35 cases)and delayed scab-cutting group(35 cases),according to types of skin flap implanting.The therapeutic effect for the two groups were evaluated with rate of infection,duration of healing,living rate of skin flap,grade of skin burning,and rate of amputate.Results In the early scab -cutting group,the rate of infection was 11%,the duration of healing was(24.0 ±6.0)days,the rate of amputate was 3%,the living rate of skin flap was 97.0%,the wound healing of class A was 94.3%.In the delayed scab-cutting group,the data were respectively 31%,(35.0 ±7.4)days,17.0%,31% and 28.6%.Compared with the data of the two groups,the difference had statistical significance(the chi-square value or T value were respectively 4.151,6.831,3. 968,31.895,3.968.All the P values was less than 0.05).Conclusion Early scab-cutting combined with varied sorts of skin flap implanting shown great benefits for treatment of wounded and facilitate rehabilitation in function of wrist,which suggest decrease rate of complications and minimized hospitalized duration.

Objective To study the value of medical thoracoscopy in the diagnosis of cryptogenic pleural effusions.Methods Medical thoracoscopy was performed in 106 patients with cryptogenic pleural effusion.Pleural biopsies were routinely performed.Results Pathologic diagnosis was obtained in 102 patients,with pleural metastasis of lung cancer in 28 cases,malignant pleural mesothelioma in 9 cases,malignant lymphoma in l case,pleural metastasis of other cancer in 3 cases,pleural tuberculosis in 40 cases,inflammation in 16 cases and sarcoidosis in 1 case.No abnormality was seen by medical thoracoscopy in 4 patients.Conclusions Medical thoracoscopy is a safe and well-tolerated procedure with high diagnostic accuracy to cryptogenic pleural effusion,and ideal pleural organ can be obtained under direct vision.The positive rate of diagnosis of pleural effusions is improved significantly by medical thoracoscopy.

Objective To analyze the distribution of reproductive tract infection(RTI)pathogens and the drug sensitivity of My-coplasma in infertile women.Methods Experimental examinations of the pathogens related to RTI were performed in 260 cases of infertile women(test group)and compared with 260 cases of pregnant women(control group).Results In test group,the positive rate of RTI pathogens was 61.2%.The top 3 pathogens were Mycoplasma (47.7%),Candida (30.0%)and bacterial vaginosis (BV)pathogens(16.7%).There were significant differences of BV pathogens,Mycoplasma,and pH value between test group and control group(P <0.05).And the differences of other pathogens and the cleanliness were not significant between test group and control group(P >0.05).The drug sensitivity rates of Ureaplasma urealyticum to doxycycline and minocin were above 90%,and which to quinolone was less than 40%.Conclusion BV pathogens and Mycoplasma infection is one of the important factors which could affect women infertility.It is necessary to strengthen the monitoring and rational use of antimicrobial agents.

Objective To observe the clinical effect of the anterolateral thigh flap in repairing defect caused by resection of malignant melanoma in the heel region.Methods 8 patients with heel malignant melanoma were trea-ted by surgery.The malignant melanoma was further diagnosed by histopathological examination.Their heel defects caused by large resection of malignant melanoma were treated by anterolateral thigh flap.Results All skin flaps sur-vived and all the patients could walk normally 1 month after operation.The recovery of the function and sense was well 6 months after operation.Compared with the another well heel,the flaps had normal texture,color and appearance of heel.8 patients had complete follow -up,ranged form 6 months to 3 years.All cases survived with neither local recur-rence nor distant metastasis.Conclusion The anterolateral thigh flap can achieve good clinical effect in treating heel defect caused by the resection of malignant melamoma.And especially apply in large skin and tissue defect of heel area.The flap can weight,wear -resisting and reduce overheating and over cold damage.The range of resection can not be restricted by local skin flap.The clinical effect is satisfied.