At present, there are the pheuomena of asking sor maney fram patients, writing out unnecessary prescription,and getting medicaine without payment among physicians. It is mot accord with the humanitarianism for patients imim China As everyone knows, the medical moraiith of health adimin'strative cadre involve that whether of not the quality of health services correspond to need of patients so, in this paper, the author discusses that how to strengthen the medical morality for health adimin's trative cadres.

Objective This study was performed to investigate whether misoprostol (PGE1 analogue) is effective for the restoration of bone loss in female rats with osteoporosis induced by ovarectomy. Methods The model of osteoporosis was established by ovariectomy in rats, and then the rats were given different doses of misoprostol. Bone mineral density(BMD), serum BGP and urine HOP/Cr were measured. Results BMD (0 26?0 03)g/cm 2 and (0 28?0 02)g/cm 2 in the misoprostal treatment groups was significantly higher than that in the control group 〔(0 23?0 02)g/cm 2, P 0 05〕 Compared with the control group 〔(3 02?0 42)?g/L, P 0 05 〕. Conclusions Results of our study demonstrate that the bone loss caused by ovariectomy was restored by misoprostol administration. The effect of misoprostol mainly is due to its promotion of bone formation.

Human CD38, a type II glycoprotein, is widely expressed in kinds of tissues and cells. It has been shown to have a wide range of effects.The multifunctional cyclase function of CD38 effects on the regulation of osteoclasts activation, which maybe important in the therapy of osteoporosis.

In the span of the interactions between cells and biomaterials, cell adhesion is the first biological behavior which has important effects on the following biological behaviors including cell migration, proliferation, and differentiation. So how to improve cell adhesion of biomaterials has become an important subject of tissue engi- neering. One of the popular methods is the surface modification of biomaterials. This article reviews the development of the research on the surface modification of biomaterials for improving cell adhesion.

BACKGROUND: The diversity of purification procedures resulting in various purities of olfactory ensheathing cells (OECs) used for grafting is considered to be relevant in the effectiveness of OECs transplant. It is important to develop a well-defined method which produces OECs of great purity and is easy to unify for the future standardization of research involving OECs.OBJECTIVE: To establish a method being easy to unify for purifying OECs to acquire highly and uniformly enriched population of OECs for standardized studies on cell transplantation.DESIGN: Randomized and controlled experiment.SETTING: Department of Orthopaedics, Affiliated Zhongda Hospital of Southeast University School of Clinical Medicine;Central Laboratory of Southeast University School of Clinical Medicine; Experimental Animal Center of Southeast University School of Clinical Medicine.MATERIALS: This experiment was carried out in the Central Laboratory of Southeast University School of Clinical Medicine from February to August 2006. Twenty-eight adult female SD rats weighing 200-250 g were selected in this study. The main reagents were detailed as follows: DMEM/F-12 (GIBCO); 2.5 g/L trypsin (GIBCO); poly-L-lysine (SIGMA); bovine pituitary extract (BPE, SIGMA); fetal bovine serum (FBS, Sijiqing Biological Agent Co., Ltd., Hangzhou);rabbit anti-low-affinity nerve growth factor receptor (anti-P75, SIGMA); biotinylated goat anti-rabbit IgG (Boster Bioengineering Co., Ltd., Wuhan); methyl thiazolyl tetrazolium (MTT) kit (SIGMA).METHODS: Primary cultures of OECs were separated from adult SD rats olfactory bulbs. At day 8 in vitro, the primary cultures were divided randomly into 4 groups, namely differential adhesion method group, immunoadsorption method group,the modified method group,and control group.①The cell suspension in the modified method group was seeded into uncoated flasks and incubated at 37 ℃ in 0.05 volume fraction of CO2 for 1 hours. The supematants were seeded into flasks that had been prepared as follows. The bottoms of these flasks were moistened with anti-P7s (1 mg/L) and were made to dry at 37 ℃, and then they were washed one time with DMEM/F-12. The supernatants were incubated on the anti-p75-treated flasks for 45 minutes at 37 ℃, 0.05 volume fraction of CO2. For removing unbound cells, the flasks were washed five times with DMEM/F-12. The bound cells were detached from the flasks with a cell scraper, centrifuged,and resuspended in D/F-10S with 105 U/L penicillin/streptomycin and 20 mg/L BPE. The cell suspension in differential anchoring method group or immunoadsorption method group was purified as previously described by Nash or Ramo'n-Cueto respectively. Three groups of cell suspensions resulted from the above three methods were seeded respectively onto poly-L-lysine-coated 24-well cell culture chambers and incubated for 14 days at 37 ℃ in 0.05 volume fraction of CO2. Without purification, the cell suspension in control group was also resuspended in D/F-10S with 105 U/L penicillin/streptomycin and 20 mg/L BPE and seeded onto poly-L-lysine-coated 24-well cell culture chambers and incubated under the same culture condition as the other groups.②Purity comparisons for the four groups were made at 2, 5, 8, 10, 12 and 14 days after the end of their respective manipulation to evaluate the effectiveness of the modified method. At per time point in each of the four groups, fifteen visual fields of cultures were selected randomly to count the At the day of 14, viabilities of OECs in the four groups were assessed by MTT assays.MAIN OUTCOME MEASURES: OECs purities at per time point and viabilities of OECs at the day of 14 in each of the four groups after the end of their respective manipulation.RESULTS:① The purities of OECs in the modified method group at each time point were greater (P＜0.05-0.01) than counterparts in the other three groups. OECs purities decreased with culture prolongation in all groups, but the changes of purities over the whole period of observation in the modified method group were the least. The last purities of OECs yielded from the modified method were still extremely great (92.1±1.2)%, whereas the parallels in the others were no more than (85.2±2.2)%.② There was no significant difference in viabilities of OECs between the modified method group and any of the others at the day of 14 (P=0.895).CONCLUSION: The modified method for purifying OECs from the adult rat olfactory bulb is highly effective without extra impairment on the viability of OECs and will be beneficial to the future standardization of research involving OECs.

Objective To evaluate the methods and results of revision surgery for posterior lumbar cage interbody fusion (cage-PLIF) with postoperative complications, and to analyse the surgical techniques for prevention of these complications. Methods From October 1996 to December 2002, 21 patients with postoperative complications of cage-PLIF underwent reoperations. There were 11 males and 10 females with an average of 43.4 years. The interval between primary and revision surgery ranged from 6 days to 1.5 years with an average of 0.6 year. 16 patients suffering from lumbar disc herniation were treated with the discecto-my and single uninstrumented cage fusion, 5 patients of lumbar spondylolisthesis were treated with cage-PLIF and pedicle screw instrumentation. The complications included cage displacement backward in 20 patients, forward in 1,and cage subsidence in 9 as well. 15 patients complained of low back pain wors-ening or leg radicular pain, of which 4 had intermittent claudication and 10 had leg numbness or weakness during rehabilitation. Revision surgery included re-implantation of the cage filled with iliac crest bone chips in 11 patients, iliac bone autograft after removal of original cages in 7 and decompression of involved nerve root witbout removal of migrated cage because of technical difficulty. Pedicle screw fixations were used in 12 and the intertransverse fusion both with autograft and allograft was added in 7. Results The mean follow-up was 14.2 months (ranged, 7 to 36 months). The cages presented slight retro-displacement in 4 patients shortly after reoperation, without involvement into spinal canal during the subsequent follow-up. Bony fusion occurred in 13 patients, and the pseudarthrosis in 3 patients without further migration of cages. The clinical symptoms relieved in 5 patients, improved in 9, no any change in 6, and worsened in 1. However, low back pain remained in 8 patients, and dysuria in 1 patiant at the last follow-up. Conclusion The results of revi-sion surgery are not satisfactory according to this study, the surgical treatments should be performed as soon as possible if conservative treatments is ineffective. The correct surgical indication and proper technical are the key of prevention of the postoperative complications.

Objective To design a new type of posterior lumbar interbody fusion cage made of NiTi shape-memory alloy and to evaluate its histological and biomechanical behavior through animal study. Methods This study contained three steps of experiments which was posterior lumbar interbody fusion using different fusion materials and devices. At the first step, 12 sheep lumbar functional spinal units were randomly divided into 4 groups with 3 specimens in each group. There was one group with autogenous iliac crest bone dowel(IG), one group with interfix lumbar cage(cage), one group with NiTi-TFC(NT) and one group served as control group. Nondestructive biomechanical tests were carried out for each group. At the second step, 15 sheep were randomly divided into three groups with 5 specimens in each group. There was one group with cage and autogenous iliac crest graft, one group with NT and iliac crest and one group served as control group. All the animals were undergone imaging study regularly to evaluate the change of the intervertebral space heights and the process of fusion. At the third step, all animal from the second step were sacrificed 6 months later for histological analysis of the operated segments. All the data were statistically analyzed using SPSS 7.0. Results The strength and axial stiffness of the autogenous iliac crest bone dowel graft group and the control group were significantly different from the NT and cage group(P

[Objective]To evaluate whether transplanted marrow mesenchymal stem cells interfered in TGF-?1 can differentiate to nucleus pulposus cells and increase the amount of proteoglycan and collagenase Ⅱ content in intervertebral discs.[Method]We used an in vivo model to investigate the feasibility of marrow mesenchymal stem cells that cultured in vitro and interfered in TGF-?1 delivery,retention,and survival in the degeneratived disc space.In 2,4,6,8 weeks we used immunohistochemical staining to determine the change of collagenase Ⅱ content;spectrophotometry to determine the change of amount of proteoglycan with Phlorglucinol;the experiment date were analyzed by SPSS 11.5 soft ware.[Result]We found MSCs could maintain viability and proliferate within the rabbit inter vertebral disc.The amount of proteoglycan and collagen Type Ⅱ content of the intervertebral in matrix synthesis in the experiment group was increased in 8 weeks.We found no changes in the modle group.[Conclusion]Our data suggest that transplanted marrow mesenchymal stem cells in vivo can survive and increase proteoglycan and collagen Type Ⅱ amount interfered in TGF-?1 in some periods,which support its potential use as a treatment of intervertebral disc degeneration.

[Summary] Lumbar disc herniation is rare in juveniles , which makes it more difficult to diagnose and treat .The prevalence , causes and risk factors , pathological changes , clinical characteristics , main treatment methods , and curative effects of lumbar disc herniation in juveniles were summarized in this review , for benefiting clinical diagnosis and treatment .

Objective To evaluate the midterm efficacy of prosthetic disc nucleus ( PDN) replacement for the treatment of lumbar disc herniation. Methods Twenty cases of lumbar disc herniation( including one case of recurrent lumbar disc herniation) were treated with PDN. The twenty cases were followed-up 5.4 ～6.2 years( mean 5.7 years). Functional,and radiographic follow-up examinations,MRI and follow-up records of all patients were reviewed carefully. Results Clinical evaluation at the end of follow-up,there were 10 cases in excellent,6 cases in good,2 cases in fair and 2 cases in poor,and good rate was 80%. One patient accepted the revision operation to remove the PDN because of device migration. One patient accepted the fusion because of adjacent segment disease. The others experienced pain relief, and resumed their normal life and work. The average Oswestry score and VAS get better significantly. Conclusion The PDN was effective in treating patients with lumbar disc herniation. However, the medium term complications of device subsidence should be taken seriously.