Objective This study was performed to investigate whether misoprostol (PGE1 analogue) is effective for the restoration of bone loss in female rats with osteoporosis induced by ovarectomy. Methods The model of osteoporosis was established by ovariectomy in rats, and then the rats were given different doses of misoprostol. Bone mineral density(BMD), serum BGP and urine HOP/Cr were measured. Results BMD (0 26?0 03)g/cm 2 and (0 28?0 02)g/cm 2 in the misoprostal treatment groups was significantly higher than that in the control group 〔(0 23?0 02)g/cm 2, P 0 05〕 Compared with the control group 〔(3 02?0 42)?g/L, P 0 05 〕. Conclusions Results of our study demonstrate that the bone loss caused by ovariectomy was restored by misoprostol administration. The effect of misoprostol mainly is due to its promotion of bone formation.

Human CD38, a type II glycoprotein, is widely expressed in kinds of tissues and cells. It has been shown to have a wide range of effects.The multifunctional cyclase function of CD38 effects on the regulation of osteoclasts activation, which maybe important in the therapy of osteoporosis.

At present, there are the pheuomena of asking sor maney fram patients, writing out unnecessary prescription,and getting medicaine without payment among physicians. It is mot accord with the humanitarianism for patients imim China As everyone knows, the medical moraiith of health adimin'strative cadre involve that whether of not the quality of health services correspond to need of patients so, in this paper, the author discusses that how to strengthen the medical morality for health adimin's trative cadres.

BACKGROUND: The diversity of purification procedures resulting in various purities of olfactory ensheathing cells (OECs) used for grafting is considered to be relevant in the effectiveness of OECs transplant. It is important to develop a well-defined method which produces OECs of great purity and is easy to unify for the future standardization of research involving OECs.OBJECTIVE: To establish a method being easy to unify for purifying OECs to acquire highly and uniformly enriched population of OECs for standardized studies on cell transplantation.DESIGN: Randomized and controlled experiment.SETTING: Department of Orthopaedics, Affiliated Zhongda Hospital of Southeast University School of Clinical Medicine;Central Laboratory of Southeast University School of Clinical Medicine; Experimental Animal Center of Southeast University School of Clinical Medicine.MATERIALS: This experiment was carried out in the Central Laboratory of Southeast University School of Clinical Medicine from February to August 2006. Twenty-eight adult female SD rats weighing 200-250 g were selected in this study. The main reagents were detailed as follows: DMEM/F-12 (GIBCO); 2.5 g/L trypsin (GIBCO); poly-L-lysine (SIGMA); bovine pituitary extract (BPE, SIGMA); fetal bovine serum (FBS, Sijiqing Biological Agent Co., Ltd., Hangzhou);rabbit anti-low-affinity nerve growth factor receptor (anti-P75, SIGMA); biotinylated goat anti-rabbit IgG (Boster Bioengineering Co., Ltd., Wuhan); methyl thiazolyl tetrazolium (MTT) kit (SIGMA).METHODS: Primary cultures of OECs were separated from adult SD rats olfactory bulbs. At day 8 in vitro, the primary cultures were divided randomly into 4 groups, namely differential adhesion method group, immunoadsorption method group,the modified method group,and control group.①The cell suspension in the modified method group was seeded into uncoated flasks and incubated at 37 ℃ in 0.05 volume fraction of CO2 for 1 hours. The supematants were seeded into flasks that had been prepared as follows. The bottoms of these flasks were moistened with anti-P7s (1 mg/L) and were made to dry at 37 ℃, and then they were washed one time with DMEM/F-12. The supernatants were incubated on the anti-p75-treated flasks for 45 minutes at 37 ℃, 0.05 volume fraction of CO2. For removing unbound cells, the flasks were washed five times with DMEM/F-12. The bound cells were detached from the flasks with a cell scraper, centrifuged,and resuspended in D/F-10S with 105 U/L penicillin/streptomycin and 20 mg/L BPE. The cell suspension in differential anchoring method group or immunoadsorption method group was purified as previously described by Nash or Ramo'n-Cueto respectively. Three groups of cell suspensions resulted from the above three methods were seeded respectively onto poly-L-lysine-coated 24-well cell culture chambers and incubated for 14 days at 37 ℃ in 0.05 volume fraction of CO2. Without purification, the cell suspension in control group was also resuspended in D/F-10S with 105 U/L penicillin/streptomycin and 20 mg/L BPE and seeded onto poly-L-lysine-coated 24-well cell culture chambers and incubated under the same culture condition as the other groups.②Purity comparisons for the four groups were made at 2, 5, 8, 10, 12 and 14 days after the end of their respective manipulation to evaluate the effectiveness of the modified method. At per time point in each of the four groups, fifteen visual fields of cultures were selected randomly to count the At the day of 14, viabilities of OECs in the four groups were assessed by MTT assays.MAIN OUTCOME MEASURES: OECs purities at per time point and viabilities of OECs at the day of 14 in each of the four groups after the end of their respective manipulation.RESULTS:① The purities of OECs in the modified method group at each time point were greater (P＜0.05-0.01) than counterparts in the other three groups. OECs purities decreased with culture prolongation in all groups, but the changes of purities over the whole period of observation in the modified method group were the least. The last purities of OECs yielded from the modified method were still extremely great (92.1±1.2)%, whereas the parallels in the others were no more than (85.2±2.2)%.② There was no significant difference in viabilities of OECs between the modified method group and any of the others at the day of 14 (P=0.895).CONCLUSION: The modified method for purifying OECs from the adult rat olfactory bulb is highly effective without extra impairment on the viability of OECs and will be beneficial to the future standardization of research involving OECs.

Objective To evaluate the midterm efficacy of prosthetic disc nucleus ( PDN) replacement for the treatment of lumbar disc herniation. Methods Twenty cases of lumbar disc herniation( including one case of recurrent lumbar disc herniation) were treated with PDN. The twenty cases were followed-up 5.4 ～6.2 years( mean 5.7 years). Functional,and radiographic follow-up examinations,MRI and follow-up records of all patients were reviewed carefully. Results Clinical evaluation at the end of follow-up,there were 10 cases in excellent,6 cases in good,2 cases in fair and 2 cases in poor,and good rate was 80%. One patient accepted the revision operation to remove the PDN because of device migration. One patient accepted the fusion because of adjacent segment disease. The others experienced pain relief, and resumed their normal life and work. The average Oswestry score and VAS get better significantly. Conclusion The PDN was effective in treating patients with lumbar disc herniation. However, the medium term complications of device subsidence should be taken seriously.

[Objective]To evaluate whether transplanted marrow mesenchymal stem cells interfered in TGF-?1 can differentiate to nucleus pulposus cells and increase the amount of proteoglycan and collagenase Ⅱ content in intervertebral discs.[Method]We used an in vivo model to investigate the feasibility of marrow mesenchymal stem cells that cultured in vitro and interfered in TGF-?1 delivery,retention,and survival in the degeneratived disc space.In 2,4,6,8 weeks we used immunohistochemical staining to determine the change of collagenase Ⅱ content;spectrophotometry to determine the change of amount of proteoglycan with Phlorglucinol;the experiment date were analyzed by SPSS 11.5 soft ware.[Result]We found MSCs could maintain viability and proliferate within the rabbit inter vertebral disc.The amount of proteoglycan and collagen Type Ⅱ content of the intervertebral in matrix synthesis in the experiment group was increased in 8 weeks.We found no changes in the modle group.[Conclusion]Our data suggest that transplanted marrow mesenchymal stem cells in vivo can survive and increase proteoglycan and collagen Type Ⅱ amount interfered in TGF-?1 in some periods,which support its potential use as a treatment of intervertebral disc degeneration.

[Objective]To evaluate short term clinic results of prosthetic disc nucleus(PDN)replacement for the treatment of lumbar disc herniation.[Method]Twenty cases of lumbar disc herniation(including one case of recurrent lumbar disc herniation)were treated with PDN from June 2003 to November 2003,including 13 males and 7 females with average age of 40.5 years.All cases were implanted with a single PDN,in which 7 cases with PR725,8 cases with PW725,and 5 cases with PDN-SOLO-7 respectively.[Result]After surgery,one patient accepted the revision operation to remove the PDN because of device migration,the others experienced pain relief.The nineteen cases were followed-up for 23～29 months(mean 26.4 months).Compared with preoperative height of intervertebral disc,it gained increase of 17.2%(P

Objective To investigate clinical features and anterior surgical results of cervical myelopathy in patients more than 70 years of age. Methods Twenty aged patients with cervical myelopathy who underwent anterior decompression with bone graft surgery were reviewed. Neurological function was assessed using a scoring system proposed by the Japanese Orthopaedic Association (JOA score). The clinical results and complications were compared with the control group which included 31 patients with multisegmental cervical myelopathy and less than 69 years old and underwent the same operation. Results The preoperative mean JOA score was 9.3 (ranged from 3 to 14) and the JOA score at latest follow-up averaged 13.4 (ranged from 8 to 17) in the aged patient group. 68 percent patients had achieved an excellent or good result and the recovery rate was 58%. 71 percent patients had achieved an excellent or good result and the recovery rate was 67% in the control group. No statistical difference was found in the excellent and good result rate or the recovery rate of JOA score between the aged group and the control group (?字2=0.04, P=0.85;t=1.12, P=0.138). The incidence of surgical related complications in the aged group was 35% (7 cases), which was considerably higher than that in the control group (3 cases, 10%) but without statistical difference (?字2=3.47, P=0.06). In the preoperative flexion-extension stress lateral radiographs, the incidence of cervical instability was higher in the control group (8 cases, 26%) than that in the aged group(1 case, 5%). Conclusion 1) Multilevel lesion induced by overcompensation for cervical instability are the probable cause of myelopathy in aged patients. 2) Anterior decompression is beneficial to improve the neurological function and life style in the aged patients with cervical myelopathy, but associated with a high incidence of surgical complications.

[Objective]To study the mechanism of posterior longitudinal ligament in cervical spine through measuring the collagen and Proteoglycan and Calcium changes.[Method]Fifteen specimens of cervical longitudinal ligament from cervical spondylotic myelopathy(CSM)and ten control specimens from corpses without cervical spondylosis were obtained.The content of the collagen was measured by Weossner method.Collagen type Ⅰ and Ⅱ were measured by Enzyme-Linked Immunosorbentassay(ELISA)method.Phloroglucinol spectrophotometer to determine the change of amount of Proteoglycan.The Calcium by Methyl-Thymes-Blue(MTB)Colorimetric Method.The specimens were treated hy Hematoxylin and Eosin(HE)stain method and by Masson stain method,the pathological changes of two groups were observed through microscopy.[Result]In CSM,as compared to the control groups,there showed a decrease in the contents of the total Collagen,Collagen type Ⅰ and Proteoglycan,and increase in the content of collagen type Ⅱ.The rate of type Ⅰ/Ⅱ in CSM was lower than that in control groups.An increase in the content of the Calcium.All of which have statistic significance(P

[Objeetive] To develop the recombinant human bone morphogenetic protein-2(rhBMP-2)loaded amorphous calcium phosphate(ACP)delayed release nano-sized material,investigate its cytotoxicity of cell,and provide a reference for the experiment of composite material in vivo.[Method]The rhBMP-2/ACP delayed release nano-sized material were prepared by chemical wet method and cultured on rabbit bone marrow-derived mesenchymal stem cells(BMSCs)in vitro.Then the adhesion,proliferation,growth and functional expression of BMSCs were measured.[Result]Cytotoxicity test demonstrated that rhBMP-2/ACP delayed release nano-sized material had not affect the percentage of cell's proliferation with material-extracted liquid cultured with BMSCs and the cytotoxicity was graded zero.The adhesion,proliferation,configuration of the cells on the surface of this material were identical to the control group.[Conclusion]It was suggested that rhBMP-2/ACP delayed release nano-sized material might have good cellular biocompatibility,no cytotoxicity and not effected the normal functional expression of BMSCs in vitro.