OBJECTIVE:To establish the quality standard of Danqitong tablets.METHODS:Salvia miltiorrhiza and Ra-dix Astragali in the formulation were identified qualitatively by TLC,and the contents of total saponins and tanshinone-Ⅱ A in the tablets were determined by visible ultraviolet spectrophotometry and HPLC,respectively.RESULTS:The TLC spots of Salvia miltiorrhiza and Radix Astragali were clear and well-separated.The linear ranges of total saponins and tanshinone-Ⅱ A were 0.056～0.12 mg?mL-1(r=0.999 3)and 0.002 019～0.032 13 mg?mL-1(r=0.999 9),respectively,and the average recovery rates were 97.61%(RSD=2.10%)and 95.74%(RSD=1.93%),respectively.CONCLUSION:The established standard is applicable for the quality control of Danqitong tablets.

OBJECTIVE:To obtain an optimum formulation of clarithromycin dispersible tablets METHODS:The preparation process was set by way of screening formulation and a formula with substitution of LS-HPC for starch was adopted RESULTS:The results showed that the substitution of LS-HPC for starch was the most preferable formulation The hardness and the friability of the tablets were improved The external apperance of unfilled corner and torn edge of the unimproved tablets was resolved CONCLUSION:Modified formulation is favorable for improving the quality of the product

BACKGROUND:Subcutaneous fat of human body is a rich reservoir of adipose derived stromal stem cells(ADSCs).ADSCs can proliferate rapidly when being cultured in vitro,and has the capacity of multi-directional differentiation.ADSCs attracted much attention in research of tissue engineered seed cells.OBJECTIVE:To isolate and culture stromal vascular fraction(SVF)cells from rabbit subcutaneous fat in vitro,and to testify whether it has multiple differentiation capacity.METHODS:SVF cells were isolated in vitro from rabbits,and cultured under standard condition.Cellular surface antigens CD44,CD45 and CD29 of passage 3 SVF cells were examined using flow cytometry.Passage 3 SVF calls were induced to differentiate into osteoblasts,chondrocytes,and lipocytes.Oil red staining was used to examine lipocyte induction.Alkaline phosphatase(ALP)staining,alizarin red staining and von Kossa staining were used to examine osteoblast induction.Type Ⅱ collagen immunohistochemical staining and type Ⅱ collagen mRNA RT-PCR were used to examine chondrocyte differentiation.RESULTS AND CONCLUSION:Primary SVF cells were multi-angular or short spindle-shaped.Passage 3 SVF calls were long spindle-shaped.Flow cytometry showed CD44+,CD29+,CD45.Oil red staining exhibited positive reaction in lipocyte induction group.ALP staining,alizarin red staining and Von kossa staining demonstrated positive reactions in osteoblast induction group.Type Ⅱ Collagen immunohistochemical staining and alcian blue staining have suggested positive reactions at 14 days of chondrogenic induction group.RT-PCR of type Ⅱ collagen mRNA test showed that the product band had strong signal at 14 days of chondrogenic induction group compared with that before induction.Above mentioned results have indicated that SVF cells isolated from rabbit subcutaneous fat have identical surface makers of stem cells,and have the ability to differentiate into lipocytes,ostsoblasts and chondrocytes in vitro by induction,and it could be concluded that the SVF cells were ADSCs.

BACKGROUND: Small intestinal submucosa (SIS) has good compatibility with cells and tissues, and has good degradabUity. It is an ideal scaffold for tissue engineering. Inducing adipose derived mesenchymal stem cells (ADSCs) seeded on SIS can construct target tissues, which has the potential to be used in clinical treatment.OBJECTIVE: To prepare decellularized porcina SIS matrix, and testify its biocompatibility with rabbit ADSCs cultured in vitro. METHODS: SIS was processed by enzyme digestion-hypertonic saline decellularization, lyophilized at low temperature, and sterilized by gamma radiation. Paraffin sections were used to observe the effect of decellularization of SIS, and the surface structures of SIS were observed by scanning electron microscope (SEM). Rabbit ADSCs were isolated and cultured, and passage 3 ADSCs were seeded onto one side or both sides of SIS. After one weak of co-culture, the cell-scaffold composites were observed.RESULTS AND CONCLUSION: SIS was white and semi-transparent film. Paraffin sections showed no cells on SIS matrix; electron microscopy showed loose weave structure of serosal surface and dense packing structure of mucosal surface. After one week of co-cultivation, plenty of ADSCs were observed on the surface of SIS. In ADSCs seeded onto one side of SIS group, a large number of cells grew on the superior surface, and few even no cells were observed on inferior surface of SIS. When ADSCs were seeded onto both sides of SIS, cells adhered to SIS in paraffin sections. Results show that enzyme digestion-hypartonic saline decellulariation can decellularize SIS completely, and SIS can support ADSCs growth.

Translational medicine has been on the rise for 20 years as a new model of biomedical research,which plays a positive role in narrowing the gap between basic research and clinical medicine and in development of medical sciences.This paper traced back the milestones of translational medicine,and analyzed the hurdles for China to develop translational medicine,namely the organization system,management model and benefit sharing.On such basis,the authors recommended on the development and strategy,especially in clinical medical institutions in China.

Objective To study the effect of Src tyrosine kinase inhibition on subcutaneously transplanted tumor of human lung adenocarcinoma in mice and its mechanism. Methods For the subcutaneously transplanted tumor model, A549 cells or PC-9 cells were inoculated into SCID mice by subcutaneous injection. Immunohistochemistry was used to show the effect of Src tyrosine kinase inhibition on proliferation index (Ki-67 staining) and microvessel density (CD31 staining) of subcutaneously transplanted tumor of human lung adenocarcinoma in mice. Results Subcutaneously transplanted tumor of PC-9 cells was sensitive to src tyrosine kinase inhibitor. There was significant difference between treatment group and control group (P <0.01). There was significant difference between the two treatment group too (P <0.01). Stopping treatment for 1 week, the inhibition rate of tumor growth were 33.19 % and 84.79 % in 10 mg·kg-1·d-1 and 50 mg·kg-1·d-1 treatment group, respectively. The same treatment was less effective to subcutaneous tumors produced by A549 cells. Treatment with 50 mg·kg-1·d-1 Src tyrosine kinase inhibitor significantly reduced the proliferation index of subcutaneously transplanted tumor produced by PC-9 cells (P<0.01) and tended to reduce the proliferation index of subcutaneously transplanted tumor produced by A549 cells (P >0.05). Treatment with 50 mg·kg-1·d-1 Src tyrosine kinase inhibitor significantly reduced micro vascular density in both PC-9 and A549 induced subcutaneous tumors (P <0.05). Conclusion Inhibition of Src tyrosine kinase could suppress the progression of subcutaneously transplanted tumor, not only by the inhibition of cell proliferation of lung adenocarcinoma cells directly, but also by the inhibition of angiogenesis indirectly.

Objective To investigate the biocompatibility of bone marrow stromal cells(BMSCs) of mice in vitro with silk fibroin materials and to explore a novel scaffold material to fabricate tissue-engineered nerve with introduction of BMSCs.Methods BMSCs were typically isolated from other cells by adherence to plastic.The mice-derived bone marrow stromal cells were cultured on the substrate of silk fibroin fibers and the cell attachment and growth during culture was observed by using light and electron microscopy.Mice-derived BMSCs were also cultured in the silk fibroin extract fluid.The cell ultrastructure was observed by transmission electron microscopy.MTT test was used to detect cell viability in different media for 12,24,48,72 hours and 7 days respectively(the test was repeated 12 times for each group).Flow cytometry was employed to detect BMSCs cell cycle and phenotypes(the test was repeated 3 times).Results BMSCs cells were tightly attached to the silk fibroin fibers and grew along the silk fibroin fibers;some of them enwrapped the silk fibroin fibers and they exhibited either a spherical or spindle shape.The results of transmission electron microscopy,MTT test and flow cytometry analysis showed that there was no significant difference between BMSCs cultured in the silk fibroin extract fluid and those in plain IMDM medium in their morphology,cell viability,proliferation and phenotypes.Conclusion These data indicate that silk fibroin has good biocompatibility with BMSCs and is also beneficial to the survival of BMSCs without exerting any significant cytotoxic effects on their phenotype;thus it's a potential scaffold material to fabricate tissue-engineered nerve with introduction of BMSCs.

OBJECTIVE:To comprehensively understand autopharmacotherapy.METHODS:The benefits and risk factors of autopharmacotherapy were analysed and the countermearsure against its risk were put forward.RESULTS&CONCLU?SION:OTC medication is safe and effective in acurate use of route dosage,but in autopharmacotherapy,there are risks as a result of influence of various factors.The safety of OTC medicines should be the matters of common concern of patients,physicians and pharmacists,and a safety mornitoring system for OTC should be established.

Gap junction has been considered allowing direct transfer of small metabolites between cells to maintain homeostasis in multicellular organisms. Deficient gap junction involved in genesis and development of many central neurological disorder, and may be a potential target for the treatment of them.

Objective To observe the effects of swimming in cold water on the functioning and structure of the peripheral nerves of diabetic rats,and to compare the effects of seawater and fresh water. Methods Forty SD rats weighing ( 250 ± 20) g were randomly divided into a normal control group (A),a diabetic model group ( B ),a seawater swimming group (C) and a fresh water swimming group (D) with 10 rats in each group.The swimming training was carried on 5 times a week for 8 weeks.At the end of the 4th and 8th week of training,caudal nerve conduction velocity (CNCV) was measured.The nerve structure of the caudal nerves was observed at the end of the 8th week. Results By the 4th week,CNCV had slowed significantly in group B compared with group A,but not in groups C and group D.Compared with group B,CNCV had increased significantly in group C.There was no significant difference in CNCV between groups C and D.At the 8th week,compared with group A,CNCV had slowed in groups B and C.Compared with group B,CNCV was significantly faster in groups C and D.However,there was no significant difference between group C and group D with regard to CNCV.At the end of the 8th week demyelination was observed in the caudal nerves under a light microscope and an electron microscope in groups B,C and D,but the demyelination was milder in groups D and C. Conclusion Swimming in cold water can prevent or delay diabetic neuropathy in diabetic rats.There was no significant difference between seawater and fresh water swimming in terms of its effect on CNCV.