Acupuncture Points ; Acupuncture Therapy ; instrumentation ; Adult ; Aged ; Female ; Humans ; Male ; Middle Aged ; Popliteal Cyst ; therapy

Objective To observe the expression of αA-and αB-crystallin in retina after blue-light exposure.Methods Forty female Wistar rats were divided randomly into 4 groups:control group,and blue-light exposure for 6,12,and 24 hours groups,with 10 rats in each group.The rats in the control group were not intervened.The other three groups of rats were exposed to blue fluorescent lights for 6,12,and 24 hours respcetively.Then the rats were kept in darkness for 12 hours.The globes were enucleated after anaesthesia.The immunohistochemistry and Western blot were performed to detect the expression of αA-and αB-crystallin in retina.Results The absorbance value(A value)of retina αA-crystallin was 1.40573±0.70748 in the control group,and were 4.317 51±0.412 97,7.397 08±1.947 90,9.634 32±2.377 61,respectively in the other 3 groups;the difference among the groups was significant(F=24.569,P<0.001).The A value of retina αB-crystallin is 0.129 36±0.033 93 in the control group,and were 0.507 17±0.117 55,7.345 43±2.292 97,4.042 26±3.890 23,respectively in the other 3 groups;the difference among the groups was significant(F=40.102,P<0.001).The results of Western blot showed that the expression of αA-and αB-crystallin in groups with blue-light exposure was obviously higher than that in the control group.Conclusions Blue light may up-regulate the expression of αA-and αB-crystallin in rats' retina.

Objective To investigate the correlation between cilioretinal artery and central visual impairment in patients with central retinal artery occlusion (CRAO).Methods A total of 32 CRAO patients (32 eyes) were selected,and results of central vision and fundus fluorescein angiography (FFA) were analyzed retrospectively.Correlation between cilioretinal arteries and degree of central visual acuity impairment was analyzed.Results In the 32 CRAO eyes,11 eyes were found with cilioretinal? arteries.Eye examination results showed that there were 10 eyes with mild visual impairment,9 eyes with moderate visual impairment and 13 eyes with severe visual impairment in the total 32 eyes.There were significant difference in the degrees of central visual impairment between CRAO patients with or without cilioretinal artery (P ＜ 0.05),and there was a significant correlation between cilioretinal artery and degree of central visual acuity impairment.Conclusion 34.4％ CRAO patients have cilioretinal artery,and cilioretinal artery in CRAO patients is associated with the degree of central visual acuity impairment.

Objective To investigate the correlation between cilioretinal artery and central visual impairment in patients with central retinal artery occlusion (CRAO).Methods A total of 32 CRAO patients (32 eyes) were selected,and results of central vision and fundus fluorescein angiography (FFA) were analyzed retrospectively.Correlation between cilioretinal arteries and degree of central visual acuity impairment was analyzed.Results In the 32 CRAO eyes,11 eyes were found with cilioretinal? arteries.Eye examination results showed that there were 10 eyes with mild visual impairment,9 eyes with moderate visual impairment and 13 eyes with severe visual impairment in the total 32 eyes.There were significant difference in the degrees of central visual impairment between CRAO patients with or without cilioretinal artery (P ＜ 0.05),and there was a significant correlation between cilioretinal artery and degree of central visual acuity impairment.Conclusion 34.4％ CRAO patients have cilioretinal artery,and cilioretinal artery in CRAO patients is associated with the degree of central visual acuity impairment.

Chronic central serous chorioretinopathy (CSC) usually demonstrates frequent recurrence,diffuse leakage and persistent subretinal fluid,which cannot be absorbed,thus lead to photoreceptor damage and poor visual acuity.As glucocorticoids have been implicated in the pathogenesis of chronic CSC,various anti-glucocorticoids oral drugs were used in the clinic to promote retinal fluid absorption and reduce the central retinal thickness of the macula and improve the vision outcomes.In addition,the 5α-reductase-specific inhibitor finasteride,the P450-3A4 inducer rifampicin,circadian rhythmic regulator melatonin,and systemic anti-inflammatory drug methotrexate have also been put into clinical trials for chronic CSC,and achieved certain effects.However,most of the clinical studies on these oral drugs were case reports,but not multi-center randomized clinical trials.The long-term effects of these oral drugs need to be observed and studied further.

Objective To investigate the protective effects of N-acetyl-L-tryptophan (L-NAT) on intestinal damage after rat hepatic ischemia reperfusion.Methods Twenty-four healthy adult rats were divided into the sham operation group (Sham),ischemia reperfusion group (IR),ischemia reperfusion and N-acetyl-L-tryptophan group(IR+L-NAT).The hepatic ischemia reperfusion model was established by occluding the afferent vessels of the left and middle lobes.The morphological structures of the small intestine were observed by hematoxylin-eosin (HE) staining.The expressions of active caspase-3,Bax and Bcl-2 were detected by immunohistochemistry staining.Results (1) In the IR group,the structure of intestinal villis was destroyed,the intestinal mucosa showed congestion and exfoliation,the epithelial cells had degeneration and necrosis,and infiltration of inflammatory cells appeared;which could be alleviated by L-NAT.(2)The immunohistochemistry showed that compared with the Sham group,the expression of active caspase-3,Bcl-2 and Bax in the IR group was increased,after L-NAT intervention,the Bax and caspase-3 expression was decreased,while the Bcl-2 expression was further increased.Conclusion L-NAT could inhibit the apoptosis of small intestinal epithelial cells caused by liver ischemic reperfusion and attenuates intestinal epithelial damage.

Objective:To manufacture one kind of biphasic calcium phosphate (BCP) ceramic by hydroxyapatite (HA) and β-tricalciumphosphate (β-TCP), and to further investigate its compatibility and its efficacy of ectopic bone formation.Methods:BCP was prepared with the ratio of HA and β-TCP at 6/4 using precipitation and H 2O 2 foaming method and then sintered at 1 100 ℃ for 3 hours. The chemical composition of BCP was investigated by X-ray diffraction(XRD). BMSCs were isolated from the bone marrow of Sprague-Dawley (SD) rat and seeded on BCP. The adhesion and morphology of BMSCs on BCP was observed under scanning electron microscope(SEM) and special staining. Cell proliferation was quantified by cell counting kit-8(CCK8) assay. Alkaline phosphatase(ALP)activity of BMSCs was measured by ALP assay kit. For further confirmation, the intramuscularly ectopic implantation models of Beagle were used, general observation, histological, and histomorphometric analyses were conducted at 4, 8, 12 weeks after implantation. Results:BCPs were successfully manufactured. XRD analysis showed the specific diffraction peaks of HA and β-TCP. SEM showed that the surface of the BCP ceramics was widely distributed with macropores and connections, and the pore walls were rough, and the micropores were evenly distributed in the macropores. Phalloidin and DAPI staining showed that the BMSCs extended and adhered to the surface of the material, and the shape gradually changed from irregularity to uniform long spindle. CCK8 method showed that although the cell viability decreased on the first day after coculture, on the third, fourth, fifth and seventh days, the cell viability gradually increased. The assay of alkaline phosphatase activity indicated that BMSCs cultured on the BCP could secrete more alkaline phosphatase on day 1 and 7 compared with the control group. BCP implanted in the muscle could generate osteoid/bone tissue at 8 weeks and 12 weeks, the number of osteoid/bone filled pores were 0.77±0.11, the percentage of osteoid/bone tissue inside the pores were 0.71±0.14.Conclusions:The BCP had a good biocompatibility and favorable efficacy of ectopic osteoinduction.

Objective:To manufacture one kind of biphasic calcium phosphate (BCP) ceramic by hydroxyapatite (HA) and β-tricalciumphosphate (β-TCP), and to further investigate its compatibility and its efficacy of ectopic bone formation.Methods:BCP was prepared with the ratio of HA and β-TCP at 6/4 using precipitation and H 2O 2 foaming method and then sintered at 1 100 ℃ for 3 hours. The chemical composition of BCP was investigated by X-ray diffraction(XRD). BMSCs were isolated from the bone marrow of Sprague-Dawley (SD) rat and seeded on BCP. The adhesion and morphology of BMSCs on BCP was observed under scanning electron microscope(SEM) and special staining. Cell proliferation was quantified by cell counting kit-8(CCK8) assay. Alkaline phosphatase(ALP)activity of BMSCs was measured by ALP assay kit. For further confirmation, the intramuscularly ectopic implantation models of Beagle were used, general observation, histological, and histomorphometric analyses were conducted at 4, 8, 12 weeks after implantation. Results:BCPs were successfully manufactured. XRD analysis showed the specific diffraction peaks of HA and β-TCP. SEM showed that the surface of the BCP ceramics was widely distributed with macropores and connections, and the pore walls were rough, and the micropores were evenly distributed in the macropores. Phalloidin and DAPI staining showed that the BMSCs extended and adhered to the surface of the material, and the shape gradually changed from irregularity to uniform long spindle. CCK8 method showed that although the cell viability decreased on the first day after coculture, on the third, fourth, fifth and seventh days, the cell viability gradually increased. The assay of alkaline phosphatase activity indicated that BMSCs cultured on the BCP could secrete more alkaline phosphatase on day 1 and 7 compared with the control group. BCP implanted in the muscle could generate osteoid/bone tissue at 8 weeks and 12 weeks, the number of osteoid/bone filled pores were 0.77±0.11, the percentage of osteoid/bone tissue inside the pores were 0.71±0.14.Conclusions:The BCP had a good biocompatibility and favorable efficacy of ectopic osteoinduction.

Objective:To manufacture one kind of biphasic calcium phosphate (BCP) ceramic by hydroxyapatite (HA) and β-tricalciumphosphate (β-TCP), and to further investigate its compatibility and its efficacy of ectopic bone formation.Methods:BCP was prepared with the ratio of HA and β-TCP at 6/4 using precipitation and H 2O 2 foaming method and then sintered at 1 100 ℃ for 3 hours. The chemical composition of BCP was investigated by X-ray diffraction(XRD). BMSCs were isolated from the bone marrow of Sprague-Dawley (SD) rat and seeded on BCP. The adhesion and morphology of BMSCs on BCP was observed under scanning electron microscope(SEM) and special staining. Cell proliferation was quantified by cell counting kit-8(CCK8) assay. Alkaline phosphatase(ALP)activity of BMSCs was measured by ALP assay kit. For further confirmation, the intramuscularly ectopic implantation models of Beagle were used, general observation, histological, and histomorphometric analyses were conducted at 4, 8, 12 weeks after implantation. Results:BCPs were successfully manufactured. XRD analysis showed the specific diffraction peaks of HA and β-TCP. SEM showed that the surface of the BCP ceramics was widely distributed with macropores and connections, and the pore walls were rough, and the micropores were evenly distributed in the macropores. Phalloidin and DAPI staining showed that the BMSCs extended and adhered to the surface of the material, and the shape gradually changed from irregularity to uniform long spindle. CCK8 method showed that although the cell viability decreased on the first day after coculture, on the third, fourth, fifth and seventh days, the cell viability gradually increased. The assay of alkaline phosphatase activity indicated that BMSCs cultured on the BCP could secrete more alkaline phosphatase on day 1 and 7 compared with the control group. BCP implanted in the muscle could generate osteoid/bone tissue at 8 weeks and 12 weeks, the number of osteoid/bone filled pores were 0.77±0.11, the percentage of osteoid/bone tissue inside the pores were 0.71±0.14.Conclusions:The BCP had a good biocompatibility and favorable efficacy of ectopic osteoinduction.

Objective:To manufacture one kind of biphasic calcium phosphate (BCP) ceramic by hydroxyapatite (HA) and β-tricalciumphosphate (β-TCP), and to further investigate its compatibility and its efficacy of ectopic bone formation.Methods:BCP was prepared with the ratio of HA and β-TCP at 6/4 using precipitation and H 2O 2 foaming method and then sintered at 1 100 ℃ for 3 hours. The chemical composition of BCP was investigated by X-ray diffraction(XRD). BMSCs were isolated from the bone marrow of Sprague-Dawley (SD) rat and seeded on BCP. The adhesion and morphology of BMSCs on BCP was observed under scanning electron microscope(SEM) and special staining. Cell proliferation was quantified by cell counting kit-8(CCK8) assay. Alkaline phosphatase(ALP)activity of BMSCs was measured by ALP assay kit. For further confirmation, the intramuscularly ectopic implantation models of Beagle were used, general observation, histological, and histomorphometric analyses were conducted at 4, 8, 12 weeks after implantation. Results:BCPs were successfully manufactured. XRD analysis showed the specific diffraction peaks of HA and β-TCP. SEM showed that the surface of the BCP ceramics was widely distributed with macropores and connections, and the pore walls were rough, and the micropores were evenly distributed in the macropores. Phalloidin and DAPI staining showed that the BMSCs extended and adhered to the surface of the material, and the shape gradually changed from irregularity to uniform long spindle. CCK8 method showed that although the cell viability decreased on the first day after coculture, on the third, fourth, fifth and seventh days, the cell viability gradually increased. The assay of alkaline phosphatase activity indicated that BMSCs cultured on the BCP could secrete more alkaline phosphatase on day 1 and 7 compared with the control group. BCP implanted in the muscle could generate osteoid/bone tissue at 8 weeks and 12 weeks, the number of osteoid/bone filled pores were 0.77±0.11, the percentage of osteoid/bone tissue inside the pores were 0.71±0.14.Conclusions:The BCP had a good biocompatibility and favorable efficacy of ectopic osteoinduction.