The chnical education to neurosurgical residents in different phase should be applied according to their different characters. In basic phase, basic theory and interest activation are important. In specific phase, the comprehensiveness and depth of training should be focused on. In community practice phase, the moral and scientific education should be strengthen. To trainees who have higher degree, the correct attitude and practice of knowledge should be emphasized.

Integrin-linked kinase (ILK) is a widely expressed protein kinase that relate to cellular growth and differentiation. It is most abundant in the heart. Recently, many researches revealed that ILK is highly relevant to cardiac response to biomechanical stresses. Also, ILK plays important roles in regulation of the occurrence and development of cardiac hypertrophy, dilated cardiomyopathy, viral myocarditis and myocardial senescence via correlation to several classical signal transduction pathway. Meanwhile, ILK functions in protection after myocardial infarction. This article will try to summarize the effects and relevant mechanism of ILK in above-mentioned aspects, overall reveals the roles of ILK in heart and its potential clinical significance.

Objective To investigate the therapeutic effect of mycophenolate mofetil (MMF) on rats with experimental autoimmune encephalomyelitis (EAE).Methods The guinea pig spinal cord homogenate (GPSCH) and complete Freund's adjuvant were used as the antigen to induce the acute EAE model in Wistar rats which were verified according to clinical scoring.Totally 36 rats were randomly and equally divided into 3 groups,that is,normal control,EAE group and MMF treatment group.In 10 d after immunization,normal saline or MMF (30 mg?kg-1?d-1) were given intragastrically for 7 d to the corresponding groups.The rats were sacrificed on the 16th day and 30th day after immunization for pathological changes of the brain and spinal cord.Clinical manifestation was scored during the experiment.Results Compared with the EAE model group,MMF-treated rats had lower clinical scores and shorter disease process.Besides,the inflammatory infiltration,demyelination and axonal damage in the central nervous system were significantly improved in MMF treatment group.Conclusion MMF is an effective drug in treating EAE,and can relieve the clinical signs and ameliorate pathological lesions in the central nervous system of acute EAE rats.

ABSTRACT:The seasonal characteristics of Japanese encephalitis (JE) in Gansu Province in 1962-2010 were analyzed in order to provide reference for making strategies of disease control and prevention .The JE data is the Epidemic Data between 1962 and 2003 from Gansu CDC ,and National Notifiable Diseases Registry System (NNDRS ,2004-2010) .Concentration ra‐tio and circular distribution method were used in this study .Results showed that the M‐value of the seasonal distribution of JE in 1962-2010 was 0 .825 9 .The corresponding peak date of JE was the 30th August (95% CI:22 July to 7 October) .The Z value of Rayleigh test was 3 506 .092 5 (P<0 .01) .In conclusion ,the concentration and circular distribution method helps us to grasp the JE epidemic peak season more accurately .There were significant seasona1 characteristics for JE in Gansu Province and the epidemic peak was from late July to early October .

8 patients, 6 males and 2 females, aged 26-56 years, with delayed neurologic sequelae following CO intoxication, were treated. The lucid intervals before the appearance of neurological sequelae varied from 5 days to 3 months, and the course of disease lasted for 7 days to 2.3 years. The clinical manifestations were mainly dementia, mutism, urinary or fecal incontinence, and gait disturbance. Among the 8 patients, clinical cure was obtained in 5 and improved in 3 after intrathecal oxygen administration.

Objective To investigate the expressions and correlation of monocarboxylate transporter-1 (MCT1) and CD147 in human gliomas,and analyzee the relationship between their expressions and prognosis.Methods Immunohistochemistry SP methods were applied to detect the expressions of MCT1 and CD147 in 28 cases of low grade gliomas and 32 cases high grade gliomas.Their expression level and correlation were analyzed statistically.Prospective cohorts were set to acquire follow-up data.Kaplan-Meier curve and Cox multi-factor model were used to analyze patients’ prognosis.Results Both CD147 and MCT1 had strong expressions in human gliomas.The positive expression rate of MCT1 and CD147 (93.8% and 90.6%) and the strong positive expression rate (73.3% and 72.4%) in high grade gliomas were significantly enhanced compared with that of low grade gliomas (positive expression rate:46.4% and 50%,strong positive expression rate:30.8% and 35.7%,P

Objective To explore the different methods of decoloration for immunohistochemical HE slides,and their influence on tissue antigen expression.Methods Antigen retrieval solution (EDTA or citric acid solution) was used in immunohistochemistry to decolor HE slides,and was compared with the method of hydrochloric acid solution decoloration in normal temperature.Results Antigen heat retrieval decolors completely.Compared with other methods,it has shorter decoloration time (1 ～ 2min),more than 90％ of completely decoloration rate,the difference was significant(x2 =27.6,23.9,all P ＜ 0.01).Meanwhile,the method dose not damage slides,and the immunohistoehemical results are exact,without background coloring and unusual coloring,antigen expression rate and intensity are high.Conclusion Antigen heat retrieval decoloration not only simplifies the coloration process,but also protects tissue antigen activity better.It is the preferable method of decoloration and protecting antigen resource,which possesses some practical value in the pathology diagnosis.

Objective: To explore the regulative role of extracellular regulated protein kinase-5 (ERK5)/Bcl-2 interacting mediator of cell death (Bim) pathway in hypothermal stimulation induced neonatal rat’s cardiomyocytes (CMs) damage and apoptosis.
Methods: CMs were cultured for hypothermal stimulation and the speciifc siRNA was used to down-regulate the ERK5 or Bim in CMs. The cell apoptosis was detected by lfow cytometry, protein expression was examined by Western blot analysis, the intracellular Ca2+, reactive oxygen species (ROS) and mitochondrial membrane potential (ΔΨm) were evaluated by lfuorescent labeling and lfow cytometry.
Results: In hypothermal stimulated CMs, ERK5 siRNA could promote Bim protein expression, but Bim siRNA could not inlfuence ERK5, while attenuated p-ERK5 expression. ERK5 siRNA induced higher apoptosis rate, while Bim siRNA could decrease such effect. ERK5 siRNA increased the intracellular Ca2+overloading, ROS activation andΔΨm damage, while Bim siRNA played the role to against those effects in hypothermal stimulated CMs.
Conclusion: Our study revealed that ERK5/Bim pathway played the important regulative roll in hypothermal stimulation induced neonatal rat’s CMs damage and apoptosis.

Objective To explore the treatment of chronic infective wounds .Methods A retrospective analysis of clinical data in 225 patients were admitted from 2000 to 2010 .Results (1) They were mainly traumatic ulcers ,pressure ulcers ,postoperative ul-cers ,diabetes ulcers ,vascular ulcers in the group ,accounted for 80 .4% (181/225) .(2) Bacterial culture positive rate was 87 .1%(196/225) ,a total of 46 kinds with 342 pathogens were cultured ,gram-positive bacteria 40 .6% (139/342);gram-negative bacteria 57 .6% (197/342);Fungi 1 .8% (6/342) .The main pathogens were S .aureus(52) ,E .coli(43) ,P .aeruginosa(44) ,Klebsiella .SPP (27) ,which were highly resistant to penicillin ,erythromycin ,ampicillin ,gentamicin ,cotrimoxazole and the multidrug resistance rate was 37 .1% (127/342) .Chronic wounds and multidrug resistant bacteria showed rapidly increasing trend from 2007 .(3) 201 pa-tients with topical antibiotic treatment ,208 patients(49 patiens underwent re-operation)underwent operations to close wounds ;213 patiens were recovery ,12 patiens had to leave hospital because economic burden .Conclusion Chronic infective wounds were affect-ed by many factors .emphasizing on debriding ,reasonablechoice ,circulative ,alternate use of antibiotics and wound bed preparation , appling surgery to close wounds in early stage could effectively control wound infection and promote wound healing .

Objective To investigate the effect of gemcitabine on the cell cycle and apoptosis of the human pancreatic cancer cell line MiaPaCa-2 with Beclinl gene silencing.Methods siRNA targeting at Beclinl gene was constructed,then it was inserted into an expression vector and transfected into MiaPaCa-2 cells.The Beclinl mRNA and protein expression were detected by RT-PCR and Western blot.Gemcitabine was used to treat MiaPaCa-2 with Beclinl gene silencing,then the cell cycle and apoptosis were detected by flow cytometry.Results The MiaPaCa-2 cells with Beclinl gene silencing were successfully constructed,and the expression of Beclin1 mRNA was decreased from 1.0 in control group to 0.295,and number of cells in S and G2 phase was decreased,but number of cells in G1 phase was increased,and there was no change in apoptosis.After gemcitabine treatment,number of cells in S phase was further decreased,but number of cells in G1,G2 phase was increased,and apoptosis was inhibited.Conclusions Beclinl gene silencing can change the cell cycle of pancreatic cancer cells MiaPaCa-2,and influence the effects of gemcitabine on cell cycle and apoptosis.