Objective To explore the situation of return-to-work (RTW) and its related factors in female injured works. Methods The da-ta of the basic information, counseling record and follow-up record after discharge were collected from social rehabilitation department in our center. The main influencing factors were analyzed by single factor analysis and two classification Logistic regression. Results 232 ob-jects were collected, among which, 149(64.2%) subjects had returned to work, and the median of absence time was 206.5 days. Single factor analysis indicated that their age, marriage, absence time and educational level were not correlated with the outcome of women injured work-ers' employment (P>0.05). However, place of social insurance, household register and injury severity correlated with the outcome (P<0.05). Conclusion Most of the female injured workers can return to work. The severity of injury, along with some system factors influence the RTW of them.

Objective: To investigate the inhibitory effect of nitidine chloride (NC) on human esophageal carcinoma cell line Eca109 and the molecular mechanism of its induction. Methods: CCK-8 method was used to detect the inhibition rate of human esophageal cancer Eca109 cells with different concentrations of NC and different intervention time. According to the result of CCK-8 method, the experiment was divided into four groups, and the concentrations of NC in each group were 0, 5, 10, and 15 μmol/L, respectively, and the drug action time was 48 h. The apoptotic rate was detected by flow cytometry with Annexin V-FITC/PI double staining. The mRNA expressions of Caspase-3, Caspase-9, and Noxa were detected by qRT-PCR. The expressions of cleaved Caspase-3 and Bcl-2, p53, and Noxa protein levels were detected by Western blotting. Results: NC had inhibitory effect on Eca109 cells in a certain range of time and dose-dependent manner. Flow cytometry showed that NC at 5 μmol/L mainly induced early apoptosis (P < 0.01); NCs at 10 and 15 μmol/L mainly induced late apoptosis (P < 0.01). qRT-PCR results showed that the mRNA expression of Caspase-3, Caspase-9 and Noxa was increased with the increase of NC concentration, of which 10 and 15 μmol/L group increased significantly. The results of Western blotting showed that the protein levels of cleaved Caspase-3, p53, and Noxa were both increased with the increase of NC concentration (P < 0.01), but the increase of Noxa was not significant in 5 μmol/L group (P < 0.01). The expression of Bcl-2 protein was decreased with the increase of NC concentration, and which was significantly higher in 10 and 15 μmol/L groups (P < 0.05, P < 0.01). Conclusion: The inhibitory effect of NC on human esophageal carcinoma Eca109 cells is mainly through apoptosis. The apoptosis of NC induced of Eca109 cells is associated with increased expression of p53 and Noxa, downregulation of Bcl-2, and activation of Caspase-3.

Objective To detect the in-vitro recovery of tetramethylpyrazine phosphate (TMPP) in microdialysis probe and to study its stability. Methods The recovery of TMPP was detected by concentration difference method (increment method and decrement method ). The effect of flow rates and concentrations on recovery was studied. Results The recovery detected by increment method was as the same as that detected by decrement method; TMPP recovery was independent of TMPP concentration in the external medium. TMPP recovery had good stability. Conclusion Microdialysis sampling can be used for the pharmacokinetic study of TMPP and decrement method (ie. retrodialysis) can be used for the determination of TMPP recovery.

Objective: When the region of interest is somewhere in the middle of the volumetric data, it will be occluded by other regions of the volumetric data and will be hard to see in the reconstruction of volume rendering. In order to provide doctor with comprehensive, visual and accurate diagnostic information, fast volume dipping of medical images on GPU is proposed in the paper. Methods: The rapid volume clipping is achieved by combining the dipping algorithm with the fast volume rendering algorithm based on GPU. The technique is based on fast volume tendering of medical images on GPU. It works as follows: The space information of the section plane is sent to the shader. Then compare the position of the volume data sets with the section plane to decide whether the data is clipped or not. Our algorithm is different from the algorithm that clipping based on depth stencil. We can see the result that reconstructed from the data sets that remained from any position as long as the section plane is defined. Results: According to the shape the users defined, our algorithm can decide which parts of the volume have to be clipped. Because of using the general graphics hardware acecleration, the proposed method achieves interactive display rate. Conclusions: The experiment shows that the algorithm is significantly fast and can meet the requirements of real-time interactive rendering on the premise of ensuring imaging quality and can be used in surgery simulation and so on.

Objective To evaluate the efficacy and safety of Liqifuwei oral liquid for prophylaxis of chemotherapy induced-constipation through a randomized controlled trial.Methods Cancer patients who met the inclusion criterias were randomly assigned to the treatment group and the control group by random number table.Liqifuwei oral liquid was prescribed 20 ml per tid from the first day of chemotherapy in the treatment group,the control group accepted the conventional treatment.Incidence and degree of chemotherapy inducedconstipation,degree and duration time of abdominal distension,anorexia and diarrhea were compared by criteria of NCI-CTC 3.0.Results A total of 100 patients were eligible,including 50 patients in the treatment group and 50 patients in the control group respectively.Incidence rate of chemotherapy induced-constipation was 48 ％ (24/50) and 98 ％ (49/50) (P ＜ 0.000 1),all patients were grade 1-3 constipation,no grade 4 and grade 5 constipation (P =0.036).Incidence rate of abdominal distension was 36 ％ (18/50) and 70 ％ (35/50)(P =0.001 3),abdominal distension degree (P =0.033),duration time of abdominal distension was (2.22±1.48)days and (3.43±1.60) days (P =0.01).Incidence rate of anorexia was 56 ％ (28/50) and 90 ％ (45/50)(P ＜ 0.000 1),there was no significance different of anorexia degree (P =0.818).One patient in treatment group underwent mild diarrhea.Conclusion The prophylaxis of Liqifuwei oral liquid for chemotherapy induced-constipation and abdominal distension is effective,which deserves large-sample studies and clinic application.

A special solid phase micro extraction ( SPME ) fibre was successfully attained by coating with a special adsorptive material on a porous stainless steel support. And the porous stainless steel support was prepared by electrochemical corrosion for the first time. The optimized erosion condition in this research was as follows: the current intensity of 30 mA, the stainless steel was etched in 2 mol/L hydrochloride solution for 1 min, then sonicated for 3 min; these procedures repeated 5 times. Meanwhile, the treated fiber coated 35 times in the sorbent solution with concentration of 5% ( m/V) in dichloromethane. The SPME fibre was applied to the analysis of gutter oil samples, and the performance of the SPME fibre for the headspace solid phase micro extraction of the exogenous impurities in gutter oil was specific, durable, stable and low-cost. The four detected trace level characteristic impurities in certain gutter oil were given as follows:42 . 7 mg/kg for acetic acid, 21. 6 mg/kg 3-butenenitrile, 71. 8 mg/kg carbon disulfide and 2. 8 mg/kg allylisothiocyanate.

Arapidscreeningmethodforthedeterminationofcarbamatepesticides(CBPs)residuesin vegetables by measuring acetylcholinesterase ( AChE ) inhibition rate using electrospray ionization mass spectrometry ( ESI-MS ) has been established. After pretreatment by QuEChERS method, sample solution reacts with AChE using acetylthiocholine as substrate. AChE inhibition rate was calculated by determination of the conversion of substrate to product ( thiocholine) using ESI-MS. The temperature, time and concentration conditions of enzymatic reactions have been optimized. The relationship between the concentration of 10 kinds of common CBPs and AChE inhibition rate was researched. Matrix effects of real vegetables were studied. The limit of detection ( LOD) , which was measured by 3 times of enzyme inhibition rate of pesticide-free vegetable samples, was 0. 01-0. 05 mg/kg. The results showed that the method was better than the current national standard method of china for rapid screening of pesticide residues and fully meet the requirements of maximum residue limits( MRL) for pesticides in food of national food safety standard. False positive results were avoided effectively due to its good ability of resistance matrix interference. The reliability was proved by analyzing vegetables with liquid chromatography-tandem mass spectrometry. The method is simple, rapid, sensitive, reliable, and can be used for the rapid, high-throughput screening of CBPs in vegetables.

Objective:To evaluate the predictive performance of the individualized drug delivery decision-making system including Smart Dose, PharmVan and JPKD on predicting the Vancomycin plasma concentration and to analyze the related factors affecting the predictive performance.Methods:The clinical data of patients who were treated with Vancomycin and received therapeutic drug monitoring (TDM) admitted to the First Affiliated Hospital of Wenzhou Medical University from January 2018 to July 2020 were retrospectively collected. Smart Dose and PharmVan were used to predict the plasma concentration of Vancomycin of the initial regimen. Smart Dose, PharmVan and JPKD were used to predict the plasma concentration of Vancomycin of the adjustment regimen for patients whose initial steady-state trough concentration were not qualified. The relative predictive error (PE) between the measured plasma concentration and predicted plasma concentration was calculated and box plotted. Mann-Whitney U test was used to evaluate the difference of the absolute value of PE (APE) predicted by each software for Vancomycin plasma concentration. The TDM results were divided into accurate prediction group (APE < 30%) and the inaccurate prediction group (APE≥30%) according to the APE value. Patients and disease characteristics including gender, age, body weight complication, Vancomycin medication and TDM results were collected from electronic medical records. Univariate analysis and multivariate Logistic regression analysis were used to screen the related factors that influence the predictive performance of Smart Dose, PharmVan and JPKD; and receiver operating characteristic curve (ROC curve) was drawn to evaluate its predictive value. Results:A total of 185 patients were enrolled, and 258 plasma concentration of Vancomycin were collected, including 185 concentrations of initial regimen and 73 concentration of adjustment regimen. There was no significant difference in the APE of the initial regimen of plasma concentration between Smart Dose and PharmVan. No significant difference in the APE of the adjustment regimen of plasma concentration was found among Smart Dose, PharmVan and JPKD. The accuracy of Smart Dose in predicting the plasma concentration of the adjustment regimen was better than that of the initial regimen [22.94% (10.50%, 36.24%) vs. 29.33% (13.07%, 47.99%), P < 0.05]. The univariate analysis of factors affecting the performance of Smart Dose in predicting the concentration of initial regimen showed that the proportion of patients with hypertension in the accurate prediction group was significantly higher than that in the inaccurate prediction group [43.3% (42/97) vs. 27.3% (24/88), P < 0.05]. The univariate analysis of factors affecting the performance of Smart Dose in predicting the concentration of adjustment regimen showed that the proportion of patients with valvular heart disease in the accurate prediction group was significantly lower than that in the inaccurate prediction group [23.4% (11/47) vs. 46.2% (12/26), P < 0.05]. The univariate analysis of factors affecting the performance of JPKD in predicting the concentration of adjustment regimen showed that the body weight of patients in the accurate prediction group was significantly higher than that in the inaccurate prediction group (kg: 62.8±14.9 vs. 54.8±12.8, P < 0.05). Multivariate Logistic regression analysis indicated that hypertension was a beneficial factor for Smart Dose to predict the initial plasma concentration of Vancomycin [odds ratio ( OR) = 0.526, 95% confidence interval (95% CI) was 0.281-0.983, P = 0.044], and low body weight was an independent risk factor for the inaccurate prediction of JPKD for adjustment regimen ( OR = 1.042, 95% CI was 1.001-1.085 , P = 0.043). ROC curve analysis indicated that the area underROC curve (AUC) of the body weight for evaluating the accuracy of JPKD in predicting Vancomycin plasma concentration was 0.663, and 95% CI was 0.529-0.796 ( P = 0.023). When the body weight was less than 55.95 kg, the risk of inaccurate prediction of JPKD in predicting Vancomycin plasma concentration was increased, and the predictive sensitivityand specificity were 75% and 60% respectively. Conclusions:There is no significant difference in the predictive performance of Smart Dose, PharmVan or JPKD on Vancomycin plasma concentration. Smart Dose had a better predictive performance for the Vancomycin plasma concentration of adjustment regimen than initial regimen. Smart Dose had a better predictive performance when the patient was concomitant with hypertension. JPKD had a poor predictive performance for low-body weight patients. The predictive performance of JPKD was decreased when the body weight was lower than 55.95 kg.

Objective To investigate the injury and disruption of anterior sacroiliac ligament (ASIL) and sacrotuberous/sacrospinous ligament complex(STL/SSL),as well as the displacement of pubic symphysis (PS) and sacroiliac joint (SIJ),associated with anterior-posterior compression (APC) of Young-Burgess type Ⅱ.Methods Test models of APC of Young-Burgess type Ⅱ were created in 10 fresh human pelvic cadaveric specimens which were randomized into 2 equal groups (n =5).The fight hemipelvis in one group was fixed to a table (the limited group) while that in the other was not (the unlimited group).At the disruption point of ASIL during external rotation of the hemipelvis,displacement of PS,separation distance between the anterior parts of the sacroiliac joint,and injury and disruption of STL/SSL were recorded.When STL/SSL was gradually made to breakdown and fracture with continuous external rotation of the hemipelvis,bony changes and injuries to the posterior pelvic ligaments were observed and recorded.Results At the failure point of ASIL,the mean displacement of PS was 23.8 ± 2.8 mm and that of SIJ was 10.9 ± 4.4 mm,showing no significant difference between the limited and unlimited groups (P ＞ 0.05);the mean external rotation angle was 40.1°± 9.8° and the mean torsion was 646.7 ± 131.5 N,showing significant differences between the limited and unlimited groups (P ＜ 0.05).At the disruption point of ASIL,obvious injuries or disruption of STL/SSL were not observed in the unlimited group but observed in the limited group.With extreme external rotation,obvious disruption of STL/SSL was not found in the unlimited group but observed in the limited group.When ASIL and STL/SSL were all fractured,the mean displacement of PS was 41.8 mm and that of SIJ was 16.8 mm in the limited group.Conclusions Since APC injury can lead to 2 situations,limited or unlimited hemipelvis,external rotation injuries to the ligaments differ in the 2 different situations.When ASIL fails,the displacement of PS will fluctuate greatly.

A high throughput screening method based on QuEChERS purification and stable isotope dilution-liquid chromatography coupled to high resolution time-of-flight mass spectrometry was developed for the simultaneous rapid determination of 86 kinds of glucocorticoids (GCs) in cosmetics.The analytes were extracted by acetonitrile, and then the extracts were purified using an improved QuEChERS method.The chromatographic separation was performed on a novel multiple chromatographic retention mechanisms column of Poroshell 120 PFP (100 mm × 2.1 mm, 2.7 μm) with gradient elution using 0.2% (V/V) acetic acid and acetonitrile as mobile phase.The accurate mass database of parent ions and mass spectra library of fragment ions of 86 GCs were established under positive ionization mode with electrospray ionization source.Based on the method described above, the qualitative identifications of the 86 GCs were accomplished without the contrast of standard substances.The results demonstrated that the linear range of this method was from 2 μg/L to 200 μg/L with good correlation coefficients of R2>0.99.The average recoveries of the 86 GCs ranged from 66.2% to 112.8%, and the relative standard deviation (RSDs) was 4.6%-13.9% at three different spiked levels.The limit of detection (LOD) and quantification (LOQ) were 0.006-0.015 mg/kg and 0.02-0.05 mg/kg, respectively.The method is simple, efficient, reliable and accurate, and is suitable for high throughput screening of 86 GCs added illegally in cosmetics.