AIM To investigate the role of Trp3 in the Ca 2+ influx induced by ? 1B AR in HEK293 cells and the effect of tyrosine kinase on it. METHODS With lipofect AMINE2000 reagent, hTrp3 cDNA was transfected to HEK293 cells and ? 1B HEK293 cells respectively. The expression of Trp3 was examined by Western blot. With Fura 2/AM spectrophoto fluorometry, Ca 2+ influx was determined. RESULTS HTrp3 was expressed endogenously in HEK293 cells, and the expression increased in hTrp3 transfected cells. Compared with untransfected cells, transfection of hTrp3 cDNA increased Ca 2+ influx induced by ? 1B AR ( P 0 05). 5～30 ?mol?L -1 genistein inhibited Ca 2+ influx induced by ? 1B AR in hTrp3 cDNA transfected cells and the maximum inhibitory rate was (75 2?12 6)% . CONCLUSION Transfection of hTrp3 cDNA increased Ca 2+ influx induced by ? 1B AR in HEK293 cells. This process was regulated by tyrosine kinase.

Objective:To investigate the predictive value of maternal peripheral blood fetal DNA, creatine kinase (CK), and alpha-fetoprotein (AFP) in pregnant women with placenta previa complicated with adhesion or implantation.Methods:From April 2018 to April 2019, 72 patients with placenta previa confirmed by cesarean section in Chengde Central Hospital were retrospectively collected. Among them, 23 patients complicated with placental adhesion were enrolled in the placenta adhesion group, 19 patients complicated with placenta implantation were in the placenta implantation group, and 30 patients with simple placenta previa were in the simple placenta previa group. The amount of fetal DNA, CK and AFP in maternal peripheral blood were measured at 20 to 27 weeks of gestation. The general data of the three groups, the amount of fetal DNA in maternal peripheral blood, CK and AFP were compared. The value of the amount of fetal DNA, CK, and AFP in maternal peripheral blood for predivting placenta previa were analyzed. At the same time, the incidence of adverse pregnancy outcomes was counted, and patients were divided into adverse pregnancy outcomes group and good pregnancy outcomes group according to pregnancy outcomes. The fetal DNA amount, CK and AFP levels in the maternal peripheral blood of the two were compared, and the factors affecting the adverse pregnancy outcome of placenta previa were analyzed.Results:The levels of fetal DNA, CK and AFP in the maternal peripheral blood of the placenta implantation group were significantly higher than those of the placenta adhesion group and the simple placenta previa group: (1 018.96 ± 442.15) copies/ml vs. (659.27 ± 320.26) copies/ml and (390.64 ± 102.53) copies/ml , (103.54 ± 26.39) U/L vs. (88.30 ± 20.65) U/L and (62.78 ± 15.84) U/L, (319.65 ± 62.14) μg/L vs. (284.62 ± 55.96) and (232.64 ± 48.62) μg/L, and the difference was statistically significant ( P<0.01). The amount of fetal DNA in maternal peripheral blood was positively correlated with CK and AFP ( r = 0.899 and 0.769, P<0.01), and CK was positively correlated with AFP ( r = 0.782, P<0.01). The AUC of maternal peripheral blood fetal DNA in predicting placenta previa complicated with placenta adhesion was 0.842, and the sensitivity and specificity were 78.26% and 83.33% respectively. The levels of fetal DNA, CK and AFP in maternal peripheral blood of patients with adverse pregnancy outcomes were higher than those of patients with good pregnancy outcomes: (928.64 ± 257.73) copies/ml vs. (460.02 ± 188.95) copies/ml, (105.83 ± 26.88) U/L vs. (66.33 ± 20.39) U/L and (292.52 ± 58.39) μg/L vs. (259.29 ± 42.65) μg/L, and the difference was statistically significant ( P<0.05). Placenta adhesion, placenta implantation, postpartum hemorrhage, maternal peripheral blood fetal DNA, CK and AFP levels were influential factors for the adverse pregnancy outcome of placenta previa ( OR = 3.544, 4.183, 3.413, 3.222, 3.109 and 3.313, 95% CI 1.905 to 6.593, 2.401 to 7.286, 1.832 to 6.359, 1.729 to 6.005, 1.659 to 5.827 and 1.831 to 5.994, P<0.01). Conclusions:The amount of fetal DNA, CK and AFP in maternal peripheral blood have a certain predictive value in placenta previa complicated with placental adhesion or implantation, and are closely related to the pregnancy outcome of patients with placenta previa. Early detection of the above indicators will help clinically to formulate reasonable intervention measures and promote the improvement of pregnancy outcomes.

Objective:To explore the calvarial critical size defect (CSD)in rats with type 2 diabetes mellitus(T2DM).Methods:T2DM model of SD rats(weighted 300-320 g)was induced by high fat and high sugar diet and low dose intraperitoneal streptozotocin (STZ)injection.The rats with T2DMand the normal controls were divided into 4 groups(n=3)respectively.Defects with the diame-ter(mm)of 2,3,4 and 5 were made on the central calvaria of each rat.General observation,X-ray examination and histological study were performed 8 weeks postoperatively.Results:In the T2DM group,only the defects of 2 mm diameter were healed completely,X-ray resistance and new bone formation were observed;the defects of 3,4 and 5 mm diameter were unhealed,X-ray transmission was observed and newly formed bone was insufficient.In the control group,the defects of 2,3 and 4 mm diameter were healed completely, X-ray resistance and new bone formation were observed;the defects of 5 mm diameter were unhealed,X-ray transmission was ob-served,newly formed bone was insufficient.Conclusion:The calvarial CSD of T2DM rat model can be defined as the defect with the diameter of 3 mm.

Objective To study the effects of binary cardiac rehabilitation (CR) composed of hospital-based and home-based CR in patients after acute myocardial infarction (AMI) evaluated by six-minute walking distance (6MWD). Methods A total of 38 post-MI patients were divided into two groups, namely conventional CR group (n=20) and binary CR group (n=18). In the first seven days, CR training was carried out in all 38 patients in our hospital, and then 20 patients were kept at CR training in the hospital (hospital-based CR group), whereas the remaining 18 patients (binary CR group) got tele-monitored walking training at home for eight weeks (binary model). Before discharge from hospital based CR or after 8 weeks CR at home, all patients received Cardiopulmonary Exercise Testing and Six-minute Walk Test, and the data of patients' body mass index (BMI),thyroid function, serum lipid metabolism and echocardiography were collected before and after CR. Results After CR, BMI and low density lipoprotein (LDL) decrease in both groups compared with those before CR[hospital-based group: BMI, (25.99±3.36)kg/m2 vs. (23.04±3.72) kg/m2,P＜0.05; LDL, (3.40±1.38) mg/dl vs. (2.04±0.73)mg/ dl,P＜0.01; binary group: BMI, (24.84±2.70) kg/m2 vs. (22.88±2.56) kg/m2,P＜0.05; LDL, (3.40±1.01) mg/dl vs. (92.11±0.37) mg/dl,P＜0.01]. After 8 weeks CR at home or hospital based CR until discharge, the anaerobic threshold (AT), maximum rate of oxygen consumption (VO2max),metabolic equivalent (MET) and 6MWD improved significantly in both groups compared with those at 7 days after CR in hospital [Hospital-based group: AT, (12.37±1.53) mL/(kg. min) vs. (14.77±1.57) mL/(kg. min); VO2max (17.87± 1.66 mL/(kg. min) vs. (20.73±2.14) mL/(kg. min); MET (5.02±0.36) vs. (6.09±0.53); 6MWD (500±53.36) m vs. (582.5±57.6) m,P＜0.01; Binary group: AT, (12.56±1.11) mL/(kg·min) vs. (14.30±1.23) mL/(kg. min); V02max, (17.28±1.38) mL/(kg. min) vs. (20.02±1.37) mL/(kg. min); MET, (5.07±0.47)vs (5.94±0.46); 6MWD,(511.4±50.96) m vs. (590.3±56.1)m,P＜0.01]. There was no significant difference in CR effects observed between two groups (P＞0.05). Conclusions In post-MI patients,a binary model of CR training improved physical capacity and was a similarly effective form of CR as a entirely hospital-based approach. A home-based tele-monitored program facilitated patients' adherence to CR. The 6 minute walk experiment is economical and good evaluation on the CR effect of binary cardiac rehabilitation.