Objective To establish a stable and useful method for culturing human osteoclast-like cells in vitro,and investigate the effect of 1?,25-(OH)2D3,M-CSF and PGE2 on osteoclasts differentiation,proliferation and activation so as to lay the foundation for further study of the biological mechanism for tooth movement.Methods The HCMNC were isolated and cultured in 24-well plate with coverslips and human dentine slices.The experiment group was cultured with 1?,25-(OH)2D3,M-CSF and PGE2,respectively,while the control group was not.The liquid was changed every 3 days and the whole culture process lasted for 7 days.The phase contrast microscopy and TRAP staining were adopted to identify osteoclast-like cells.Results On the 3rd day the monocytes began to fuse and on the 7th day positive multinucleated cells could be seen with TRAP staining,but absorption pit was not formed on the dentin slices.The group with 1?,25-(OH)2D3 had the largest number of osteoclast-like cells.Conclusion After the monocytes in UCB are cultured by 1?,25-(OH)2D3,M-CSF,PGE2 induction,they can turn into TRAP(+) multinucleate osteoclast-like cells,the 1?,25-(OH)2D3 10-8mol/L being the most effective.

OBJECTIVE:To establish a method for determining the content of paeoniflorin in Xiaohe syrup. METHODS:HPLC method was adopted. The Waters SunFire C18 column was used with the mobile phase of acetonitrile-0.1% phosphoric acid(12∶88, V/V)at the flow rate of 1.2 ml/min;the detection wavelength was 230 nm with column temperature at 30℃. Sample size was 10μl . R E-SULTS:There was a good linear relationship between the amount of paeoniflorin and peak area in the range of 0.061 0-1 . 2 2 0 8 μg (r=0.999 9). The RSDs of precision,stability and reproducibility tests were all less than 2% and the average recovery was 98.77%(RSD=1.09%,n=6). CONCLUSIONS:The method is simple,rapid and accurate and can be used for the content deter-mination of paeoniflorin in Xiaohe Syrup.

ObjectiveTo explore the effects of nutritional intervention and individualized nursing on nutritional risk, undernutrition and quality of life (QOL) in end-stage renal disease (ESRD) patients with peritoneal dialysis. MethodsA total of 104 ESRD patients with peritoneal dialysis who met the inclusion criteria of the protocol were enrolled and randomized to receive nutritional intervention plus individualized nursing ( study group, n =52 analyzed) or self-diet plus routine nursing ( control group, n =50 analyzed) for 6 months. Nutritional risk, anthropometry, and QOL of the two groups were analyzed pre- and post-nutritional intervention. ResultsBaseline data were comparable in the two groups. Prevalences of nutritional risk and undernutrition in study group were significant lower than those in control group after the intervention ( nutritional risk: 32.6％ vs. 56.0％, P =0.028 ;undernutrition: 15.4％ vs. 34.0％, P =0.038). The decrease of grip strength in study group between pre- and post-study was significantly less than that in control group [( - 1.6 ± 0.9 ) kg vs. ( - 9.9 ± 1.4 ) kg, P =0.001], but there were no significant differences in other parameters related to anthropometry, including triceps skin-fold thickness, upper arm circumference, and arm muscle circumference ( all P ＞ 0.05 ). The QOL score significantly increased in study group after intervention but decreased in control group. The differences of renal disease and dialysis-related scores ( △KDTA: 2.5 ± 4.4 vs.- 7.9 ± 7.4, P =0.001 ) and general condition scores (△SF-36 : 3.4 ±4.1 vs.- 6.8 ± 6.3, P =0.001 ) before and after intervention were significantly different between two groups. ConclusionNutrition intervention and individualized nursing may help to improve the nutritional status and QOL in ESRD patients with peritoneal dialysis.

Objective To study the effect of continuously compressive pressure(CCP) and human periodontal ligament cells(HPDLCs) on the differentiation of osteoclast-like cells(OLC) induced from umbilical cord blood cells in vitro,and to investigate the role of continuously compressive pressure and human periodontal ligament cells in alveolar bone rebuilding during orthordontic tooth movement.Methods Mononucleared cells of umbilical cord blood(HCMNCs) were separated by density gradient centrifugation,HPDLCs were isolated from human periodontal ligament by explanting enzymatic digestion with trypsin and collagenase.We also established transwell co-culture system with HCMNCs in the lower layer and HPDLCs in the upper layer.Group A: HCMNCs and HPDLCs were co-cultured with 150 kPa CCP for 1.5 hours on the model.Group B: only HCMNCs were cultured with the same CCP as Group A.Groups A'and B' were the respective control group of Groups A and B with no CCP exerted.The cell appearance was observed under the phase contrast microscope,and its identification was performed by histochemical analysis of tartrater-resistant acid phosphatase(TRAP).The capacity of bone resorption of the cell was assessed by lacunae forming ability on bone slice.Results HCMNCs in Group A began to fuse on the 2nd day,More positive multinucleated cells could be seen with TRAP staining and cortical bone pit formation on the 3rd day.Only a few multinucleated cells formed in the other groups,with no cortical bone pit formation.Conclusion HCMNCs can fuse into multinucleated OLC under CCP with the induction of HPDLCs.

Objective To study comparatively reduction and prevention of surgery-induced peritoneal adhesion by methylene blue and hyaluronate sodium. Methods 46 patients with acute diffuse peritonitis or adhesion intestinal ileus were divided at random into 3 groups: methylene blue ( MB) group, hyaluronate sodium (HS) group and control ( C ) group. The alteration of postoperation recovery,serum MDA, TNF? and erythrocytes SOD in patients were investigated. Results The recuperation in group MB was better than in group C (P

Objective To investigate the relationship between the parameter of P100 elicited by pattern stimuli, spatial frequency and visual acuity in the posttraumatic eyes. Methods 81 eyes sustained blunt injury in outpatients were enrolled from West China Hospital, Sichuan University. The control group was composed of 199 eyes without disease or trauma. Different P100 wave including amplitude and latency were recorded in each eye stimulated by reversal pattern with 5 - sized checkerboards. The data were processed by SPSS to describe the relationship between the visual acuity and the parameter of P100 wave. A linear and regression analysis was done in the statistic procedure. Results There was a high correlation between spatial frequency ( X) and the log visual acuity ( Y). Regression equations were deduced for evaluating the visual acuity. There was a correlation between the latency of P100 wave (X) and the log visual acuity (Y) in all the examined eyes. Conclusion The established regression equations can be used to evaluate the visual acuity as an accurate and objective method. There is a promising future of its application in the forensic clinical authentication of visual function.

Objective:To study the distribution of the literature and the trend of international research of proliferative vitreoretinopathy (PVR) in 10 years from 2009 to 2018.Methods:Using Web of Science database of the American Institute of Scientific Information as the data source to collect literature on proliferative vitreoretinopathy published from 2009 to 2018 and a statistical analysis on the distribution of the publication time, nation and funding agency was made to figure out the institution, periodical, author with highest citations respectively among the collected literature.Meanwhile, the bibliometric analysis software Citespace and VOSviewer were employed to carry out the distribution analysis and cluster analysis of keywords as well as the co-citation cluster analysis.Results:A total of 905 articles related to PVR were included, mainly in English, most of which were written by American authors, and the number of literature published by Chinese authors was ranked the second.Among all the article funding institutions, the National Natural Science Foundation of China ranked NO.1 with the most funded articles.Citations increased year by year since 2009 and increased obviously since 2013.The journal with the highest citations was Investigative Ophthalmology & Visual Science, and the research institution with the highest citations was Harvard University.The statistical analysis of the citation quantity also showed that the top five authors with the highest citations were all from Massachusetts Eye and Ear, Department of Ophthalmology, Harvard Medical School.The results of cluster analysis showed that there were 41 high-frequency subject terms, and they were mainly grouped in 4 categories, which were etiology, risk factors, molecular mechanism, treatment and management of PVR.Keywords network visualization and overlay visualization both showed that etiology and risk factors were the focus of current research, while epithelial mesenchymal transition, transforming growth factor-β and fibrosis were new research fields in recent years.The results of citation cluster analysis showed there were 12 co-citation clusters. Conclusions:The literature of PVR shows an upward trend, and the researches focus on the molecular mechanism, prevention, and neuroprotection of PVR, which may be the future PVR research direction.

Objective: To examine the effect of conjugated linoleic acid (CLA) on lipid metabolism and genes expression of fatty acid transport protein(FATP)and acyl-CoA synthase(ACS) in white adipose tissue of rats. Method: Male Wistar rats were randomly divided into control group, high-fat group and high fat +CLA group (0.75,1.50,3.00 g/100g bw). By reverse transcription polymerse chain reaction (RT-PCR) technique the expression levels of FATP, ACS and peroxisome proliferator-activated receptor-?(PPAR?) mRNA were detected. Results: Serum FFA,insulin and glucose levels of rats fed with high fat diet were higher than those of control group. Supplement of CLA can decrease serum FFA, insulin and glucose levels of obese rats, and increase expression of FATP,ACS and PPAR?mRNA in adipose tissue of obese rats. Conclusion: CLA can improve insulin resistance of obese rat and increase expression of FATP,ACS mRNA, possibly acting through activating PPAR?.

Human embryonic stem cell (hES cells) lines can be derived from the inner cell mass (ICM) of preimplantation blastocysts.hES cells are commonly defined as undifferentiated pluripotent cells that can proliferate and have the capacity of both self-renewal and differentiation into one or more types of specialized cells.hES cells remain undifferentiated when culture on feeder layers,such as murine embryonic fibroblasts (MEFs).It is believed that various factors secreted from feeder layers are necessary to prevent hES cell from differentiation.In this review,we will summarize the advantages and disadvantages of various types of feeder cells by which a reference for future research will be provided.

Objective To analyze the latency of posture evoked response of normal lower limb muscle in different stimulations and explore its influencing factors. Methods The normal lower limb was induced to produce postural evoked response by the dynamic posturography through two kinds of perturbations, the supporting surface rotation stimulation (Toes-up and Toes-down) and the horizontal perturbation stimulation (Forward and Backward). The latencies of tibialis anterior muscle and gastrocnemius muscle were recorded by surface electromyography acquisition system. The differences of the left and right limb, gen-der and height on the latency of postural evoked response were analyzed. Results (1) Under the Toes-up and Backward perturbation, the latency of tibialis anterior muscle was longer than gastrocnemius muscle;under the Toes-down and Forward perturbation, the latency of gastrocnemius muscle was longer than tib-ialis anterior muscle. (2) The latencies of left limb and right limb had no significant difference. (3) The la-tency in male was longer than that in female. (4) The latency gradually increased with the increase of height. Conclusion In the postural evoked response, different perturbations, gender and height have sig-nificant impacts on the latency of posture evoked response of lower limb muscle. However, the effect of height and gender should be not considered referring to the same individual.